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COELENTERAZINE
10122. 331.4. Yellow solid soluble in MeOH and EtOH. There are over a dozen of coelenterazine analogs that have been synthesized, many of which are now commercially available from Biotium. These coelenterazine analogs have different properties in terms of emission wavelength, cell membrane permeability and quantum efficiency.LYSOVIEW™ DYES
LysoView™ dyes label lysosomes in live cells, and are available with blue, green, visible red, and far-red fluorescence. No-wash, fluorescent staining of lysosomes in live cells. Non-toxic for real-time live-cell imaging. Lower background than LyosoTracker® dyes. Dye options for super-resolution imaging. Blue, green, red, orfar-red
EVERBRITE™ MOUNTING MEDIUM Product Description. Biotium’s EverBrite™ mounting medium is a revolutionary antifade mounting medium optimally formulated for preserving fluorescence of our CF® dyes and other fluorochromes for your immunofluorescence (IF) imaging applications. Antifade mounting medium optimally formulated to prevent photobleaching of fluorophores. NUCSPOT® LIVE CELL NUCLEAR STAINS NucSpot® Live 488 Ex/Em: 500/515 nm. NucSpot® Live 650 Ex/Em: 650/675 nm. NucSpot® Live 488 stains nuclei with green fluorescence for the FITC channel, while NucSpot® Live 650 has far-red fluorescence for the Cy®5 channel. NucSpot® Live 650 dye also is compatible with super-resolution imaging by SIM or STED, and has beenvalidated in
VIAFLUOR® SE CELL PROLIFERATION KITS ViaFluor® SE Cell Proliferation Kits use amine-reactive dyes to covalently label cells throughout the cell cytoplasm and intracellular compartments for fixable fluorescent staining. Cell proliferation dyes are commonly used to monitor cell division by flow cytometry. The dyes also can be used to stably label cells to image cell morphology, or GELRED® NUCLEIC ACID GEL STAIN GelRed® is an ultra sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. Compatible with downstream gel purification, restriction digest, sequencing, and cloning. GLOMELT™ THERMAL SHIFT PROTEIN STABILITY KIT GloMelt™ Thermal Shift Protein Stability Kit. Kit for fluorescence-based measurement of protein unfolding or thermal stability in thermal shift assay, also called Protein Thermal Shift™, differential scanning fluorimetry, or Thermofluor assay. Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugatesCF®350 DYE
CF®350 Dye. CF®350 is a blue fluorescent dye. Learn More. 300 400 500 600 700 800 900 Wavelength (nm) CF350. Technical Summary. Abs/Em Maxima: 347/448 nm. Extinction coefficient: 18,000. Molecular weight: ~ 496. Excitation source: UV.DILINOLEYL DII
Dilinoleyl DiI
(1,1′-dilinoleyl-3,3,3′,3′-tetramethylindocarbocynanine perchlorate), also known as FAST DiI™, has identical absorption and emission spectra as DiI™ (# 60010) . Due to its unsaturated hydrocarbon chains, dilinoleyl DiI has a much faster lateral diffusion rate in cell membranes than DiI . This property makes dilinoleyl BIOTIUM - GLOWING PRODUCTS FOR SCIENCEPRODUCTSTECHNOLOGIESSPECIAL OFFERSABOUT USSPECTRA VIEWERNUCLEIC ACID GEL STAINS Features. Kit sizes for labeling ≤5 -100 ug antibody. Maxi size for labeling 1 mg of IgG also available. Choice of 29 CF® Dye colors, biotin, FITC, or haptens. Less than 30 seconds of hands-on time. 30 minutes total reaction time. No purification required, 100% antibody recovery. Compatible with BSA, gelatin, ascites. Learn More.COELENTERAZINE
10122. 331.4. Yellow solid soluble in MeOH and EtOH. There are over a dozen of coelenterazine analogs that have been synthesized, many of which are now commercially available from Biotium. These coelenterazine analogs have different properties in terms of emission wavelength, cell membrane permeability and quantum efficiency.LYSOVIEW™ DYES
LysoView™ dyes label lysosomes in live cells, and are available with blue, green, visible red, and far-red fluorescence. No-wash, fluorescent staining of lysosomes in live cells. Non-toxic for real-time live-cell imaging. Lower background than LyosoTracker® dyes. Dye options for super-resolution imaging. Blue, green, red, orfar-red
EVERBRITE™ MOUNTING MEDIUM Product Description. Biotium’s EverBrite™ mounting medium is a revolutionary antifade mounting medium optimally formulated for preserving fluorescence of our CF® dyes and other fluorochromes for your immunofluorescence (IF) imaging applications. Antifade mounting medium optimally formulated to prevent photobleaching of fluorophores. NUCSPOT® LIVE CELL NUCLEAR STAINS NucSpot® Live 488 Ex/Em: 500/515 nm. NucSpot® Live 650 Ex/Em: 650/675 nm. NucSpot® Live 488 stains nuclei with green fluorescence for the FITC channel, while NucSpot® Live 650 has far-red fluorescence for the Cy®5 channel. NucSpot® Live 650 dye also is compatible with super-resolution imaging by SIM or STED, and has beenvalidated in
VIAFLUOR® SE CELL PROLIFERATION KITS ViaFluor® SE Cell Proliferation Kits use amine-reactive dyes to covalently label cells throughout the cell cytoplasm and intracellular compartments for fixable fluorescent staining. Cell proliferation dyes are commonly used to monitor cell division by flow cytometry. The dyes also can be used to stably label cells to image cell morphology, or GELRED® NUCLEIC ACID GEL STAIN GelRed® is an ultra sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. Compatible with downstream gel purification, restriction digest, sequencing, and cloning. GLOMELT™ THERMAL SHIFT PROTEIN STABILITY KIT GloMelt™ Thermal Shift Protein Stability Kit. Kit for fluorescence-based measurement of protein unfolding or thermal stability in thermal shift assay, also called Protein Thermal Shift™, differential scanning fluorimetry, or Thermofluor assay. Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugatesCF®350 DYE
CF®350 Dye. CF®350 is a blue fluorescent dye. Learn More. 300 400 500 600 700 800 900 Wavelength (nm) CF350. Technical Summary. Abs/Em Maxima: 347/448 nm. Extinction coefficient: 18,000. Molecular weight: ~ 496. Excitation source: UV.DILINOLEYL DII
Dilinoleyl DiI
(1,1′-dilinoleyl-3,3,3′,3′-tetramethylindocarbocynanine perchlorate), also known as FAST DiI™, has identical absorption and emission spectra as DiI™ (# 60010) . Due to its unsaturated hydrocarbon chains, dilinoleyl DiI has a much faster lateral diffusion rate in cell membranes than DiI . This property makes dilinoleyl FLUORESCENT PROTEINS, NUCLEOTIDES & OTHER CONJUGATES CF®555-ddCTP. From: $375 Sizes: 25 nmol Catalog #: 40031. CF®555-ddCTP. An orange-red fluorescent 3′-end chain terminator in Sanger sequencing. Select Size 25 nmol. Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarilyunavailable.
NUCSPOT® LIVE CELL NUCLEAR STAINS NucSpot® Live 488 Ex/Em: 500/515 nm. NucSpot® Live 650 Ex/Em: 650/675 nm. NucSpot® Live 488 stains nuclei with green fluorescence for the FITC channel, while NucSpot® Live 650 has far-red fluorescence for the Cy®5 channel. NucSpot® Live 650 dye also is compatible with super-resolution imaging by SIM or STED, and has beenvalidated in
VIAFLUOR® CELL PROLIFERATION KITS ViaFluor® 405, a high-performance cell proliferation dye. Developed by Biotium, ViaFluor® 405 is an excellent choice for a cell proliferation dye. Unlike some other dyes, it is non-toxic to cells (Fig. 6), and does not interfere with cellular processes such as cell cycle progression or T cell activation. The signal is bright andstable
EVERBRITE™ MOUNTING MEDIUM BACK. Near-IR CF® Dye Annexin V Conjugates; Near-IR CytoplasmicMembrane Stains
GLOMELT™ THERMAL SHIFT PROTEIN STABILITY KIT GloMelt™ Thermal Shift Protein Stability Kit. Kit for fluorescence-based measurement of protein unfolding or thermal stability in thermal shift assay, also called Protein Thermal Shift™, differential scanning fluorimetry, or Thermofluor assay. Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates RESAZURIN CELL VIABILITY ASSAY KIT (ALAMARBLUE™) Resazurin Cell Viability Assay offers a simple, rapid, reliable, sensitive, safe and cost-effective measurement of cell viability. This assay has excellent performance compared to other resazurin-based cell proliferation kits such as alamarBlue®, PrestoBlue®, or CellTiter-Blue®. Single-step, homogeneous, high-throughput cellquantitation
EVERBRITE™ HARDSET MOUNTING MEDIUM WITH NUCSPOT® NUCLEAR Unique wetset or hardset antifade mounting media with the novel far-red nuclear counterstain NucSpot® 640. LIVE-OR-DYE NUCFIX™ RED STAINING KIT Live-or-Dye NucFix™ Red is a reactive, cell membrane impermeant dye that specifically stains the nuclei of dead cells. The dye is able to enter into dead cells that have compromised membrane integrity and covalently label the cell nucleus, allowing for clear differentiation of live and dead cells by either microscopy or flow cytometry.CELLULAR STAINS
2 • www.biotium.com Overview of Organelle & Cytoskeleton Stains Cytoskeleton • Live cell microtubule stains (p. 10) • Phalloidin conjugates for fixed cells (p. WHAT DOES VAPING VITAMIN E ACETATE DO TO YOUR LUNGS? Vitamin E acetate and your lungs. One of the proposed mechanisms for EVALI associated lung damage is the potential interaction of vitamin E with pulmonary surfactant (PS) in your lungs. PS is a phospholipid layer that lines the tiny air sacs in your lungs. These air sacs, known as alveoli, allow your bloodstream to exchange carbon dioxidewith
BIOTIUM - GLOWING PRODUCTS FOR SCIENCEPRODUCTSTECHNOLOGIESSPECIAL OFFERSABOUT USSPECTRA VIEWERNUCLEIC ACID GEL STAINS Features. Kit sizes for labeling ≤5 -100 ug antibody. Maxi size for labeling 1 mg of IgG also available. Choice of 29 CF® Dye colors, biotin, FITC, or haptens. Less than 30 seconds of hands-on time. 30 minutes total reaction time. No purification required, 100% antibody recovery. Compatible with BSA, gelatin, ascites. Learn More.CF®405S DYE
Highly water soluble and pH-insensitive. CF®405S Dye. Improved brightness & photostability for the 405 nm laser line. Learn More. 300 400 500 600 700 800 900 Wavelength (nm) CF405S. Technical Summary. Abs/Em Maxima: 404/431 nm. Extinction coefficient: 33,000. Molecularweight: ~ 1,169.
COELENTERAZINE
10122. 331.4. Yellow solid soluble in MeOH and EtOH. There are over a dozen of coelenterazine analogs that have been synthesized, many of which are now commercially available from Biotium. These coelenterazine analogs have different properties in terms of emission wavelength, cell membrane permeability and quantum efficiency.LYSOVIEW™ DYES
LysoView™ dyes label lysosomes in live cells, and are available with blue, green, visible red, and far-red fluorescence. No-wash, fluorescent staining of lysosomes in live cells. Non-toxic for real-time live-cell imaging. Lower background than LyosoTracker® dyes. Dye options for super-resolution imaging. Blue, green, red, orfar-red
GLOMELT™ THERMAL SHIFT PROTEIN STABILITY KIT GloMelt™ Thermal Shift Protein Stability Kit. Kit for fluorescence-based measurement of protein unfolding or thermal stability in thermal shift assay, also called Protein Thermal Shift™, differential scanning fluorimetry, or Thermofluor assay. Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates EVERBRITE™ MOUNTING MEDIUM Product Description. Biotium’s EverBrite™ mounting medium is a revolutionary antifade mounting medium optimally formulated for preserving fluorescence of our CF® dyes and other fluorochromes for your immunofluorescence (IF) imaging applications. Antifade mounting medium optimally formulated to prevent photobleaching of fluorophores. FURAPTRA (MAG-FURA-2), TETRASODIUM SALT Product Description. Mag-Fura-2 is a UV-excitable fluorescent indicator for magnesium with a K d of 1.9 mM. Similar to Fura-2, the excitation wavelength of Mag-Fura-2 undergoes a blue shift from 369 nm to 330 nm. Mag-Fura-2 also responds to Ca 2+ but with a significantly higher K d than Fura-2 for Ca 2+.GELRED® | BIOTIUM
PAGE GelRed® is a non-toxic, non-mutagenic red DNA gel stain specifically formulated to stain DNA in polyacrylamide gels. PAGE GelRed® can be imaged using a 254 nm UV transilluminator with an ethidium bromide filter. Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC,PerCP, HRP, or
PMAXX™ DYE, 20 MM IN H2O PMAxx™ dye is a DNA modifier used for viability PCR, invented by scientists at Biotium. PMAxx™ is a new and improved version of our popular viability dye PMA (propidium monoazide). Like PMA, PMAxx™ is a photo-reactive dye that binds to DNA with high affinity. Upon photolysis with visible light, PMAxx™ dye becomes covalentlyattached to DNA.
PROTOCOL: STAINING CELLS WITH HOECHST OR DAPI NUCLEAR Live cell staining by medium exchange. Add the dye to complete culture medium. Use Hoechst dyes at 1 ug/mL or DAPI at 10 ug/mL. Note: DAPI or Hoechst can be combined with other fluorescent probes. Remove culture medium from the cells and replace with medium containing dye. Incubate cells at room temperature or 37°C for 5-15 minutes, then image. BIOTIUM - GLOWING PRODUCTS FOR SCIENCEPRODUCTSTECHNOLOGIESSPECIAL OFFERSABOUT USSPECTRA VIEWERNUCLEIC ACID GEL STAINS Features. Kit sizes for labeling ≤5 -100 ug antibody. Maxi size for labeling 1 mg of IgG also available. Choice of 29 CF® Dye colors, biotin, FITC, or haptens. Less than 30 seconds of hands-on time. 30 minutes total reaction time. No purification required, 100% antibody recovery. Compatible with BSA, gelatin, ascites. Learn More.CF®405S DYE
Highly water soluble and pH-insensitive. CF®405S Dye. Improved brightness & photostability for the 405 nm laser line. Learn More. 300 400 500 600 700 800 900 Wavelength (nm) CF405S. Technical Summary. Abs/Em Maxima: 404/431 nm. Extinction coefficient: 33,000. Molecularweight: ~ 1,169.
COELENTERAZINE
10122. 331.4. Yellow solid soluble in MeOH and EtOH. There are over a dozen of coelenterazine analogs that have been synthesized, many of which are now commercially available from Biotium. These coelenterazine analogs have different properties in terms of emission wavelength, cell membrane permeability and quantum efficiency.LYSOVIEW™ DYES
LysoView™ dyes label lysosomes in live cells, and are available with blue, green, visible red, and far-red fluorescence. No-wash, fluorescent staining of lysosomes in live cells. Non-toxic for real-time live-cell imaging. Lower background than LyosoTracker® dyes. Dye options for super-resolution imaging. Blue, green, red, orfar-red
GLOMELT™ THERMAL SHIFT PROTEIN STABILITY KIT GloMelt™ Thermal Shift Protein Stability Kit. Kit for fluorescence-based measurement of protein unfolding or thermal stability in thermal shift assay, also called Protein Thermal Shift™, differential scanning fluorimetry, or Thermofluor assay. Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates EVERBRITE™ MOUNTING MEDIUM Product Description. Biotium’s EverBrite™ mounting medium is a revolutionary antifade mounting medium optimally formulated for preserving fluorescence of our CF® dyes and other fluorochromes for your immunofluorescence (IF) imaging applications. Antifade mounting medium optimally formulated to prevent photobleaching of fluorophores. FURAPTRA (MAG-FURA-2), TETRASODIUM SALT Product Description. Mag-Fura-2 is a UV-excitable fluorescent indicator for magnesium with a K d of 1.9 mM. Similar to Fura-2, the excitation wavelength of Mag-Fura-2 undergoes a blue shift from 369 nm to 330 nm. Mag-Fura-2 also responds to Ca 2+ but with a significantly higher K d than Fura-2 for Ca 2+.GELRED® | BIOTIUM
PAGE GelRed® is a non-toxic, non-mutagenic red DNA gel stain specifically formulated to stain DNA in polyacrylamide gels. PAGE GelRed® can be imaged using a 254 nm UV transilluminator with an ethidium bromide filter. Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC,PerCP, HRP, or
PMAXX™ DYE, 20 MM IN H2O PMAxx™ dye is a DNA modifier used for viability PCR, invented by scientists at Biotium. PMAxx™ is a new and improved version of our popular viability dye PMA (propidium monoazide). Like PMA, PMAxx™ is a photo-reactive dye that binds to DNA with high affinity. Upon photolysis with visible light, PMAxx™ dye becomes covalentlyattached to DNA.
PROTOCOL: STAINING CELLS WITH HOECHST OR DAPI NUCLEAR Live cell staining by medium exchange. Add the dye to complete culture medium. Use Hoechst dyes at 1 ug/mL or DAPI at 10 ug/mL. Note: DAPI or Hoechst can be combined with other fluorescent probes. Remove culture medium from the cells and replace with medium containing dye. Incubate cells at room temperature or 37°C for 5-15 minutes, then image. FLUORESCENT PROTEINS, NUCLEOTIDES & OTHER CONJUGATES CF®555-ddCTP. From: $375 Sizes: 25 nmol Catalog #: 40031. CF®555-ddCTP. An orange-red fluorescent 3′-end chain terminator in Sanger sequencing. Select Size 25 nmol. Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarilyunavailable.
LYSOVIEW™ DYES
LysoView™ dyes label lysosomes in live cells, and are available with blue, green, visible red, and far-red fluorescence. No-wash, fluorescent staining of lysosomes in live cells. Non-toxic for real-time live-cell imaging. Lower background than LyosoTracker® dyes. Dye options for super-resolution imaging. Blue, green, red, orfar-red
GELRED® | BIOTIUM
PAGE GelRed® is a non-toxic, non-mutagenic red DNA gel stain specifically formulated to stain DNA in polyacrylamide gels. PAGE GelRed® can be imaged using a 254 nm UV transilluminator with an ethidium bromide filter. Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC,PerCP, HRP, or
MIX-N-STAIN™ ANTIBODY LABELING KITS Mix-n-Stain™ Antibody Labeling Kits use revolutionary technology that dramatically simplifies the process of antibody labeling. The labeling is covalent and stable, and is available in many label options: choice of 29 CF® dyes, biotin, FITC, fluorescent protein tags (ie, R-PE, APC, PerCP, APC-CF®750T), enzymes (ie, GoX, AP, HRP), Digoxigenin, or dinitrophenol (DNP), as well as Mix-n-Stain VIAFLUOR® CELL PROLIFERATION KITS ViaFluor® 405, a high-performance cell proliferation dye. Developed by Biotium, ViaFluor® 405 is an excellent choice for a cell proliferation dye. Unlike some other dyes, it is non-toxic to cells (Fig. 6), and does not interfere with cellular processes such as cell cycle progression or T cell activation. The signal is bright andstable
EVERBRITE™ MOUNTING MEDIUM Unlike VECTASHIELD® wetset, EverBrite™ does not quench the fluorescence of cyanine-based dyes. Methanol-fixed HeLa cells in a 96-well coversglass bottom dish were stained with mouse anti-tubulin antibody followed by Cy®5 goat anti-mouse secondary antibody, then placed in the indicated mounting medium and imaged by confocal microscopy using the same laser and gain settings. VIAFLUOR® SE CELL PROLIFERATION KITS ViaFluor® SE Cell Proliferation Kits use amine-reactive dyes to covalently label cells throughout the cell cytoplasm and intracellular compartments for fixable fluorescent staining. Cell proliferation dyes are commonly used to monitor cell division by flow cytometry. The dyes also can be used to stably label cells to image cell morphology, or PMAXX™ DYE, 20 MM IN H2O PMAxx™ dye is a DNA modifier used for viability PCR, invented by scientists at Biotium. PMAxx™ is a new and improved version of our popular viability dye PMA (propidium monoazide). Like PMA, PMAxx™ is a photo-reactive dye that binds to DNA with high affinity. Upon photolysis with visible light, PMAxx™ dye becomes covalentlyattached to DNA.
GELRED® NUCLEIC ACID GEL STAIN GelRed® is an ultra sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. Compatible with downstream gel purification, restriction digest, sequencing, and cloning. OXAZOLE GOLD (SYBR® GOLD), 10,000X IN DMSO Oxazole Gold, also known as SYBR® Gold, is an ultra sensitive nucleic acid gel stain with ex/em of 300, 495/537 nm (with DNA). BIOTIUM - GLOWING PRODUCTS FOR SCIENCEPRODUCTSTECHNOLOGIESSPECIAL OFFERSABOUT USSPECTRA VIEWERNUCLEIC ACID GEL STAINS Features. Kit sizes for labeling ≤5 -100 ug antibody. Maxi size for labeling 1 mg of IgG also available. Choice of 29 CF® Dye colors, biotin, FITC, or haptens. Less than 30 seconds of hands-on time. 30 minutes total reaction time. No purification required, 100% antibody recovery. Compatible with BSA, gelatin, ascites. Learn More. CONTACT US | BIOTIUM Biotium Corporate Headquarters. Biotium, Inc. 46117 Landing Parkway Fremont, CA 94538. Phone: 510-265-1027 Customer & Order Support Representatives Available: Monday – Friday, 7:00am – 4:00pmLYSOVIEW™ DYES
LysoView™ dyes label lysosomes in live cells, and are available with blue, green, visible red, and far-red fluorescence. No-wash, fluorescent staining of lysosomes in live cells. Non-toxic for real-time live-cell imaging. Lower background than LyosoTracker® dyes. Dye options for super-resolution imaging. Blue, green, red, orfar-red
CF®555 DYE
A bright and photostable orange-red dye. Learn More. 300 400 500 600 700 800 900 Wavelength (nm) CF555. Technical Summary. Abs/Em Maxima: 555/565 nm. Extinction coefficient: 150,000. Molecular weight: ~ 901. Excitation laser line: 532 nm or 568 nm. Direct replacement for: AlexaFluor®.
FURAPTRA (MAG-FURA-2), TETRASODIUM SALT Product Description. Mag-Fura-2 is a UV-excitable fluorescent indicator for magnesium with a K d of 1.9 mM. Similar to Fura-2, the excitation wavelength of Mag-Fura-2 undergoes a blue shift from 369 nm to 330 nm. Mag-Fura-2 also responds to Ca 2+ but with a significantly higher K d than Fura-2 for Ca 2+. FAST PROBE MASTER MIX Fast Probe Master Mix is designed for fluorescent probe-based PCR applications. It is compatible with probes such as a TaqMan®, BHQ®, and Molecular Beacon. However for most PCR applications we recommend the Forget-Me-Not™ Universal Probe Master Mix, for superior activity. 2X probe-based qPCR master mix for fast cycling protocols. GLOMELT™ THERMAL SHIFT PROTEIN STABILITY KIT GloMelt™ Thermal Shift Protein Stability Kit. Kit for fluorescence-based measurement of protein unfolding or thermal stability in thermal shift assay, also called Protein Thermal Shift™, differential scanning fluorimetry, or Thermofluor assay. Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates VIAFLUOR® SE CELL PROLIFERATION KITS ViaFluor® SE Cell Proliferation Kits use amine-reactive dyes to covalently label cells throughout the cell cytoplasm and intracellular compartments for fixable fluorescent staining. Cell proliferation dyes are commonly used to monitor cell division by flow cytometry. The dyes also can be used to stably label cells to image cell morphology, or TYRAMIDES AND TYRAMIDE SIGNAL AMPLIFICATION KITS Horseradish peroxidase (HRP) catalyzes the covalent reaction of tyramide to tyrosine residues. In the tyramide signal amplification technique, immunostaining is performed with HRP-conjugated antibodies or streptavidin. Subsequent reaction with fluorescent tyramide causes large numbers of fluorescent dye molecules to be deposited in the vicinity of the antibody, generating dramatically stronger WHAT DOES VAPING VITAMIN E ACETATE DO TO YOUR LUNGS? Vitamin E acetate and your lungs. One of the proposed mechanisms for EVALI associated lung damage is the potential interaction of vitamin E with pulmonary surfactant (PS) in your lungs. PS is a phospholipid layer that lines the tiny air sacs in your lungs. These air sacs, known as alveoli, allow your bloodstream to exchange carbon dioxidewith
BIOTIUM - GLOWING PRODUCTS FOR SCIENCEPRODUCTSTECHNOLOGIESSPECIAL OFFERSABOUT USSPECTRA VIEWERNUCLEIC ACID GEL STAINS Features. Kit sizes for labeling ≤5 -100 ug antibody. Maxi size for labeling 1 mg of IgG also available. Choice of 29 CF® Dye colors, biotin, FITC, or haptens. Less than 30 seconds of hands-on time. 30 minutes total reaction time. No purification required, 100% antibody recovery. Compatible with BSA, gelatin, ascites. Learn More. CONTACT US | BIOTIUM Biotium Corporate Headquarters. Biotium, Inc. 46117 Landing Parkway Fremont, CA 94538. Phone: 510-265-1027 Customer & Order Support Representatives Available: Monday – Friday, 7:00am – 4:00pmLYSOVIEW™ DYES
LysoView™ dyes label lysosomes in live cells, and are available with blue, green, visible red, and far-red fluorescence. No-wash, fluorescent staining of lysosomes in live cells. Non-toxic for real-time live-cell imaging. Lower background than LyosoTracker® dyes. Dye options for super-resolution imaging. Blue, green, red, orfar-red
CF®555 DYE
A bright and photostable orange-red dye. Learn More. 300 400 500 600 700 800 900 Wavelength (nm) CF555. Technical Summary. Abs/Em Maxima: 555/565 nm. Extinction coefficient: 150,000. Molecular weight: ~ 901. Excitation laser line: 532 nm or 568 nm. Direct replacement for: AlexaFluor®.
FURAPTRA (MAG-FURA-2), TETRASODIUM SALT Product Description. Mag-Fura-2 is a UV-excitable fluorescent indicator for magnesium with a K d of 1.9 mM. Similar to Fura-2, the excitation wavelength of Mag-Fura-2 undergoes a blue shift from 369 nm to 330 nm. Mag-Fura-2 also responds to Ca 2+ but with a significantly higher K d than Fura-2 for Ca 2+. FAST PROBE MASTER MIX Fast Probe Master Mix is designed for fluorescent probe-based PCR applications. It is compatible with probes such as a TaqMan®, BHQ®, and Molecular Beacon. However for most PCR applications we recommend the Forget-Me-Not™ Universal Probe Master Mix, for superior activity. 2X probe-based qPCR master mix for fast cycling protocols. GLOMELT™ THERMAL SHIFT PROTEIN STABILITY KIT GloMelt™ Thermal Shift Protein Stability Kit. Kit for fluorescence-based measurement of protein unfolding or thermal stability in thermal shift assay, also called Protein Thermal Shift™, differential scanning fluorimetry, or Thermofluor assay. Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates VIAFLUOR® SE CELL PROLIFERATION KITS ViaFluor® SE Cell Proliferation Kits use amine-reactive dyes to covalently label cells throughout the cell cytoplasm and intracellular compartments for fixable fluorescent staining. Cell proliferation dyes are commonly used to monitor cell division by flow cytometry. The dyes also can be used to stably label cells to image cell morphology, or TYRAMIDES AND TYRAMIDE SIGNAL AMPLIFICATION KITS Horseradish peroxidase (HRP) catalyzes the covalent reaction of tyramide to tyrosine residues. In the tyramide signal amplification technique, immunostaining is performed with HRP-conjugated antibodies or streptavidin. Subsequent reaction with fluorescent tyramide causes large numbers of fluorescent dye molecules to be deposited in the vicinity of the antibody, generating dramatically stronger WHAT DOES VAPING VITAMIN E ACETATE DO TO YOUR LUNGS? Vitamin E acetate and your lungs. One of the proposed mechanisms for EVALI associated lung damage is the potential interaction of vitamin E with pulmonary surfactant (PS) in your lungs. PS is a phospholipid layer that lines the tiny air sacs in your lungs. These air sacs, known as alveoli, allow your bloodstream to exchange carbon dioxidewith
VIAFLUOR® CELL PROLIFERATION KITS ViaFluor® 405, a high-performance cell proliferation dye. Developed by Biotium, ViaFluor® 405 is an excellent choice for a cell proliferation dye. Unlike some other dyes, it is non-toxic to cells (Fig. 6), and does not interfere with cellular processes such as cell cycle progression or T cell activation. The signal is bright andstable
FAST PROBE MASTER MIX Fast Probe Master Mix is designed for fluorescent probe-based PCR applications. It is compatible with probes such as a TaqMan®, BHQ®, and Molecular Beacon. However for most PCR applications we recommend the Forget-Me-Not™ Universal Probe Master Mix, for superior activity. 2X probe-based qPCR master mix for fast cycling protocols. NUCSPOT® LIVE CELL NUCLEAR STAINS NucSpot® Live 488 Ex/Em: 500/515 nm. NucSpot® Live 650 Ex/Em: 650/675 nm. NucSpot® Live 488 stains nuclei with green fluorescence for the FITC channel, while NucSpot® Live 650 has far-red fluorescence for the Cy®5 channel. NucSpot® Live 650 dye also is compatible with super-resolution imaging by SIM or STED, and has beenvalidated in
AUTHORIZED DISTRIBUTORS Authorized Distributors. Biotium only sells directly to US-based customers, but we have a large network of international distributors who take care of our international order requests. Please choose your region on the map below and contact your local distributors to receive a price quote to purchase our products. If you are unable to locate a DCTP CF® DYE CONJUGATES CF® Dye dCTP can be used to synthesize labeled DNA probes for in situ hybridization, microarray, or blotting applications. Synthesize exceptionally bright and photostable fluorescent DNA probes. Detect by fluorescence microscopy, flow cytometry, or in gel. Compatible with fixed cells or tissue sections. Choice of 9 CF® dye colors from green TYRAMIDES AND TYRAMIDE SIGNAL AMPLIFICATION KITS Horseradish peroxidase (HRP) catalyzes the covalent reaction of tyramide to tyrosine residues. In the tyramide signal amplification technique, immunostaining is performed with HRP-conjugated antibodies or streptavidin. Subsequent reaction with fluorescent tyramide causes large numbers of fluorescent dye molecules to be deposited in the vicinity of the antibody, generating dramatically stronger GLOMELT™ THERMAL SHIFT PROTEIN STABILITY KIT GloMelt™ Thermal Shift Protein Stability Kit. Kit for fluorescence-based measurement of protein unfolding or thermal stability in thermal shift assay, also called Protein Thermal Shift™, differential scanning fluorimetry, or Thermofluor assay. Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates VIAFLUOR® SE CELL PROLIFERATION KITS ViaFluor® SE Cell Proliferation Kits use amine-reactive dyes to covalently label cells throughout the cell cytoplasm and intracellular compartments for fixable fluorescent staining. Cell proliferation dyes are commonly used to monitor cell division by flow cytometry. The dyes also can be used to stably label cells to image cell morphology, or VERSABLOT™ TOTAL PROTEIN NORMALIZATION KITS VersaBlot™ Total Protein Normalization Kits are designed for rapid and sensitive protein detection on SDS-PAGE gels and western blot (WB) membranes. The proteins are covalently labeled with near-infrared CF® Dyes so that after electrophoresis, the bands can be immediately visualized by fluorescence detection without gel staining.CELLULAR STAINS
2 • www.biotium.com Overview of Organelle & Cytoskeleton Stains Cytoskeleton • Live cell microtubule stains (p. 10) • Phalloidin conjugates for fixed cells (p. BIOTIUM - GLOWING PRODUCTS FOR SCIENCEPRODUCTSTECHNOLOGIESSPECIAL OFFERSABOUT USSPECTRA VIEWERNUCLEIC ACID GEL STAINS Features. Kit sizes for labeling ≤5 -100 ug antibody. Maxi size for labeling 1 mg of IgG also available. Choice of 29 CF® Dye colors, biotin, FITC, or haptens. Less than 30 seconds of hands-on time. 30 minutes total reaction time. No purification required, 100% antibody recovery. Compatible with BSA, gelatin, ascites. Learn More. CONTACT US | BIOTIUM Biotium Corporate Headquarters. Biotium, Inc. 46117 Landing Parkway Fremont, CA 94538. Phone: 510-265-1027 Customer & Order Support Representatives Available: Monday – Friday, 7:00am – 4:00pmLYSOVIEW™ DYES
LysoView™ dyes label lysosomes in live cells, and are available with blue, green, visible red, and far-red fluorescence. No-wash, fluorescent staining of lysosomes in live cells. Non-toxic for real-time live-cell imaging. Lower background than LyosoTracker® dyes. Dye options for super-resolution imaging. Blue, green, red, orfar-red
CF®555 DYE
A bright and photostable orange-red dye. Learn More. 300 400 500 600 700 800 900 Wavelength (nm) CF555. Technical Summary. Abs/Em Maxima: 555/565 nm. Extinction coefficient: 150,000. Molecular weight: ~ 901. Excitation laser line: 532 nm or 568 nm. Direct replacement for: AlexaFluor®.
FURAPTRA (MAG-FURA-2), TETRASODIUM SALT Product Description. Mag-Fura-2 is a UV-excitable fluorescent indicator for magnesium with a K d of 1.9 mM. Similar to Fura-2, the excitation wavelength of Mag-Fura-2 undergoes a blue shift from 369 nm to 330 nm. Mag-Fura-2 also responds to Ca 2+ but with a significantly higher K d than Fura-2 for Ca 2+. FAST PROBE MASTER MIX Fast Probe Master Mix is designed for fluorescent probe-based PCR applications. It is compatible with probes such as a TaqMan®, BHQ®, and Molecular Beacon. However for most PCR applications we recommend the Forget-Me-Not™ Universal Probe Master Mix, for superior activity. 2X probe-based qPCR master mix for fast cycling protocols. GLOMELT™ THERMAL SHIFT PROTEIN STABILITY KIT GloMelt™ Thermal Shift Protein Stability Kit. Kit for fluorescence-based measurement of protein unfolding or thermal stability in thermal shift assay, also called Protein Thermal Shift™, differential scanning fluorimetry, or Thermofluor assay. Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates VIAFLUOR® SE CELL PROLIFERATION KITS ViaFluor® SE Cell Proliferation Kits use amine-reactive dyes to covalently label cells throughout the cell cytoplasm and intracellular compartments for fixable fluorescent staining. Cell proliferation dyes are commonly used to monitor cell division by flow cytometry. The dyes also can be used to stably label cells to image cell morphology, or TYRAMIDES AND TYRAMIDE SIGNAL AMPLIFICATION KITS Horseradish peroxidase (HRP) catalyzes the covalent reaction of tyramide to tyrosine residues. In the tyramide signal amplification technique, immunostaining is performed with HRP-conjugated antibodies or streptavidin. Subsequent reaction with fluorescent tyramide causes large numbers of fluorescent dye molecules to be deposited in the vicinity of the antibody, generating dramatically stronger WHAT DOES VAPING VITAMIN E ACETATE DO TO YOUR LUNGS? Vitamin E acetate and your lungs. One of the proposed mechanisms for EVALI associated lung damage is the potential interaction of vitamin E with pulmonary surfactant (PS) in your lungs. PS is a phospholipid layer that lines the tiny air sacs in your lungs. These air sacs, known as alveoli, allow your bloodstream to exchange carbon dioxidewith
BIOTIUM - GLOWING PRODUCTS FOR SCIENCEPRODUCTSTECHNOLOGIESSPECIAL OFFERSABOUT USSPECTRA VIEWERNUCLEIC ACID GEL STAINS Features. Kit sizes for labeling ≤5 -100 ug antibody. Maxi size for labeling 1 mg of IgG also available. Choice of 29 CF® Dye colors, biotin, FITC, or haptens. Less than 30 seconds of hands-on time. 30 minutes total reaction time. No purification required, 100% antibody recovery. Compatible with BSA, gelatin, ascites. Learn More. CONTACT US | BIOTIUM Biotium Corporate Headquarters. Biotium, Inc. 46117 Landing Parkway Fremont, CA 94538. Phone: 510-265-1027 Customer & Order Support Representatives Available: Monday – Friday, 7:00am – 4:00pmLYSOVIEW™ DYES
LysoView™ dyes label lysosomes in live cells, and are available with blue, green, visible red, and far-red fluorescence. No-wash, fluorescent staining of lysosomes in live cells. Non-toxic for real-time live-cell imaging. Lower background than LyosoTracker® dyes. Dye options for super-resolution imaging. Blue, green, red, orfar-red
CF®555 DYE
A bright and photostable orange-red dye. Learn More. 300 400 500 600 700 800 900 Wavelength (nm) CF555. Technical Summary. Abs/Em Maxima: 555/565 nm. Extinction coefficient: 150,000. Molecular weight: ~ 901. Excitation laser line: 532 nm or 568 nm. Direct replacement for: AlexaFluor®.
FURAPTRA (MAG-FURA-2), TETRASODIUM SALT Product Description. Mag-Fura-2 is a UV-excitable fluorescent indicator for magnesium with a K d of 1.9 mM. Similar to Fura-2, the excitation wavelength of Mag-Fura-2 undergoes a blue shift from 369 nm to 330 nm. Mag-Fura-2 also responds to Ca 2+ but with a significantly higher K d than Fura-2 for Ca 2+. FAST PROBE MASTER MIX Fast Probe Master Mix is designed for fluorescent probe-based PCR applications. It is compatible with probes such as a TaqMan®, BHQ®, and Molecular Beacon. However for most PCR applications we recommend the Forget-Me-Not™ Universal Probe Master Mix, for superior activity. 2X probe-based qPCR master mix for fast cycling protocols. GLOMELT™ THERMAL SHIFT PROTEIN STABILITY KIT GloMelt™ Thermal Shift Protein Stability Kit. Kit for fluorescence-based measurement of protein unfolding or thermal stability in thermal shift assay, also called Protein Thermal Shift™, differential scanning fluorimetry, or Thermofluor assay. Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates VIAFLUOR® SE CELL PROLIFERATION KITS ViaFluor® SE Cell Proliferation Kits use amine-reactive dyes to covalently label cells throughout the cell cytoplasm and intracellular compartments for fixable fluorescent staining. Cell proliferation dyes are commonly used to monitor cell division by flow cytometry. The dyes also can be used to stably label cells to image cell morphology, or TYRAMIDES AND TYRAMIDE SIGNAL AMPLIFICATION KITS Horseradish peroxidase (HRP) catalyzes the covalent reaction of tyramide to tyrosine residues. In the tyramide signal amplification technique, immunostaining is performed with HRP-conjugated antibodies or streptavidin. Subsequent reaction with fluorescent tyramide causes large numbers of fluorescent dye molecules to be deposited in the vicinity of the antibody, generating dramatically stronger WHAT DOES VAPING VITAMIN E ACETATE DO TO YOUR LUNGS? Vitamin E acetate and your lungs. One of the proposed mechanisms for EVALI associated lung damage is the potential interaction of vitamin E with pulmonary surfactant (PS) in your lungs. PS is a phospholipid layer that lines the tiny air sacs in your lungs. These air sacs, known as alveoli, allow your bloodstream to exchange carbon dioxidewith
VIAFLUOR® CELL PROLIFERATION KITS ViaFluor® 405, a high-performance cell proliferation dye. Developed by Biotium, ViaFluor® 405 is an excellent choice for a cell proliferation dye. Unlike some other dyes, it is non-toxic to cells (Fig. 6), and does not interfere with cellular processes such as cell cycle progression or T cell activation. The signal is bright andstable
FAST PROBE MASTER MIX Fast Probe Master Mix is designed for fluorescent probe-based PCR applications. It is compatible with probes such as a TaqMan®, BHQ®, and Molecular Beacon. However for most PCR applications we recommend the Forget-Me-Not™ Universal Probe Master Mix, for superior activity. 2X probe-based qPCR master mix for fast cycling protocols. NUCSPOT® LIVE CELL NUCLEAR STAINS NucSpot® Live 488 Ex/Em: 500/515 nm. NucSpot® Live 650 Ex/Em: 650/675 nm. NucSpot® Live 488 stains nuclei with green fluorescence for the FITC channel, while NucSpot® Live 650 has far-red fluorescence for the Cy®5 channel. NucSpot® Live 650 dye also is compatible with super-resolution imaging by SIM or STED, and has beenvalidated in
AUTHORIZED DISTRIBUTORS Authorized Distributors. Biotium only sells directly to US-based customers, but we have a large network of international distributors who take care of our international order requests. Please choose your region on the map below and contact your local distributors to receive a price quote to purchase our products. If you are unable to locate a DCTP CF® DYE CONJUGATES CF® Dye dCTP can be used to synthesize labeled DNA probes for in situ hybridization, microarray, or blotting applications. Synthesize exceptionally bright and photostable fluorescent DNA probes. Detect by fluorescence microscopy, flow cytometry, or in gel. Compatible with fixed cells or tissue sections. Choice of 9 CF® dye colors from green TYRAMIDES AND TYRAMIDE SIGNAL AMPLIFICATION KITS Horseradish peroxidase (HRP) catalyzes the covalent reaction of tyramide to tyrosine residues. In the tyramide signal amplification technique, immunostaining is performed with HRP-conjugated antibodies or streptavidin. Subsequent reaction with fluorescent tyramide causes large numbers of fluorescent dye molecules to be deposited in the vicinity of the antibody, generating dramatically stronger GLOMELT™ THERMAL SHIFT PROTEIN STABILITY KIT GloMelt™ Thermal Shift Protein Stability Kit. Kit for fluorescence-based measurement of protein unfolding or thermal stability in thermal shift assay, also called Protein Thermal Shift™, differential scanning fluorimetry, or Thermofluor assay. Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates VIAFLUOR® SE CELL PROLIFERATION KITS ViaFluor® SE Cell Proliferation Kits use amine-reactive dyes to covalently label cells throughout the cell cytoplasm and intracellular compartments for fixable fluorescent staining. Cell proliferation dyes are commonly used to monitor cell division by flow cytometry. The dyes also can be used to stably label cells to image cell morphology, or VERSABLOT™ TOTAL PROTEIN NORMALIZATION KITS VersaBlot™ Total Protein Normalization Kits are designed for rapid and sensitive protein detection on SDS-PAGE gels and western blot (WB) membranes. The proteins are covalently labeled with near-infrared CF® Dyes so that after electrophoresis, the bands can be immediately visualized by fluorescence detection without gel staining.CELLULAR STAINS
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ANTIFIX™ ANTIGEN RETRIEVAL BUFFER A highly optimized heat-induced epitope retrieval (HIER) reagent with exceptional performance for a wide variety of targets in FFPE tissue. AntiFix™ is non-toxic, non-flammable, and may be applied at neutralpH.
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EXPLORE NEAR-IR CF® DYES Industry-leading performance over other long wavelength dyes. 20% OFF ALL LIVE-OR-DYE™ KITS For a limited time receive 20% off our Live-or-Dye™ Fixable Viability Staining Kits. Innovative dyes for science. Expert solutions for scientists.PRODUCT CATALOG
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TECHNOLOGY HIGHLIGHT: MIX-N-STAIN™ CF® DYE ANTIBODY LABELING KITSImmunofluorescence
staining of rat testis with mouse anti-tubulin and CF®488A goat anti-mouse (min x rat) (microtubules, green), CF®555 Mix-n-Stain™ labeled mouse anti-ZO1 (tight junctions, red) and CF®640R phalloidin (actin filaments, cyan). BRIGHT AND STABLE CONJUGATES IN 30 MINUTES Mix-n-Stain™ CF® Dye Antibody Labeling Kits allow you to label ≤5 ug up to 100 ug of your antibody in just 30 minutes, with minimal hands-on time and no purification.FEATURES
* Kit sizes for labeling ≤5 -100 ug antibody * Maxi size for labeling 1 mg of IgG also available * Choice of 29 CF® Dye colors, biotin, FITC, or haptens * Less than 30 seconds of hands-on time * 30 minutes total reaction time * No purification required, 100% antibody recovery * Compatible with BSA, gelatin, ascitesLearn More
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