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ANTI HUMAN J CHAIN ANTIBODY, CLONE MC19-9 Mouse anti Human J chain antibody, clone Mc19-9 reacts with human J chain as tested by ELISA using purified human Ig fragments as the solid phase target antigen. Mouse anti Human J chain antibody, clone Mc19-9 does not detect J chain in intact IgM in ELISA assays due tosteric hindrance.
LEUCOPERM (ACCESSORY REAGENT) Create mode – the default mode when you create a requisition and PunchOut to Bio-Rad. You can create and edit multiple shopping carts Edit mode – allows you to edit or modify an existing requisition (prior to submitting). You will be able to modify only the cart that you have PunchedOut to, and won't have access to any other carts ANTI RITUXIMAB ANTIBODY, CLONE MB2A4 Rat Anti-Rituximab Antibody, clone MB2A4, is an anti-idiotypic antibody that recognizes the monoclonal antibody drug rituximab. The antibody can be used to measure the levels of rituximab and biosimilar products in bioanalytical assays. ANTI HUMAN CD68 ANTIBODY, CLONE KP1 Figure A. FITC conjugated Mouse anti Human CD14 and Alexa Fluor 647 conjugated Mouse IgG1 isotype control ().Figure B. FITC conjugated Mouse anti Human CD14 and Alexa Fluor 647 conjugated Mouse anti Human CD68 (MCA5709A647).All experiments performed on Leucoperm fixed and permeabilized human peripheral blood gated on single cell mononuclearcells.
ANTI DOG MHC CLASS II MONOMORPHIC ANTIBODY, CLONE YKIX334 Rat anti Dog MHC Class II Monomorphic antibody, clone YKIX334.2 recognizes a monomorphic epitope on canine MHC Class II and was classified at the First Canine Leucocyte Antigen Workshop (Cobbold et al. 1994).The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections.In dogs, this is referred to as the dog leukocyte antigen (DLA) region. ANTI PIG CD203A ANTIBODY, CLONE PM18-7 Mouse anti Pig CD203a, clone PM18-7 recognizes porcine CD203a, originally clustered as SWC9 at the Second International Swine CD Workshop (Dominguez et al. 1998) and later identified as the porcine homologue of human ecto-nucleotidepyrophosphatase / phosphodiesterase 1 or ENPP1 (Petersen et al. 2007). Mouse anti Pig CD203a was originally reported to immunoprecipitate two bands, one of ~;205 RABBIT ANTI HUMAN SMAD3 ANTIBODY Rabbit anti Human SMAD3 antibody recognizes Mothers against decapentaplegic homolog 3 (SMAD3), a 50 kDa transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase, SMAD3 functions in cell proliferation, differentiation and development. Upon stimulation, SMAD3 oligomerizes with SMAD4 and translocates into the nucleus where it binds to DNA and transcription factors. ANTI PIG SLA CLASS II DR ANTIBODY, CLONE 2E9/13 Mouse anti Pig SLA Class II DR antibody, clone 2E9/13 recognizes SLA DR molecules which are expressed on all B cells, antigen presenting cells and on certain subsets of resting and activated T cells. Mouse anti Pig SLA Class II DR antibody, clone 289/13 reacts with lymphocytes from all outbred and miniature pigs so far tested, suggesting that it recognizes a monomorphic determinant of porcine SPYTAG & SPYCATCHER PRODUCTS MATERIAL SAFETY DATASHEETS Bio-Rad provides Material Safety Datasheets (MSDS) that comply with the latest regulations from the European Union*. Material Safety Datasheets (MSDS) for Bio-Rad products can be found by refering to the Health and Safety Information section of the specific product-related datasheets found on our website. Please contact antibody_tech_uk@bio-rad.com if you have difficulty finding the MSDSfor a
ANTI HUMAN J CHAIN ANTIBODY, CLONE MC19-9 Mouse anti Human J chain antibody, clone Mc19-9 reacts with human J chain as tested by ELISA using purified human Ig fragments as the solid phase target antigen. Mouse anti Human J chain antibody, clone Mc19-9 does not detect J chain in intact IgM in ELISA assays due tosteric hindrance.
LEUCOPERM (ACCESSORY REAGENT) Create mode – the default mode when you create a requisition and PunchOut to Bio-Rad. You can create and edit multiple shopping carts Edit mode – allows you to edit or modify an existing requisition (prior to submitting). You will be able to modify only the cart that you have PunchedOut to, and won't have access to any other carts ANTI RITUXIMAB ANTIBODY, CLONE MB2A4 Rat Anti-Rituximab Antibody, clone MB2A4, is an anti-idiotypic antibody that recognizes the monoclonal antibody drug rituximab. The antibody can be used to measure the levels of rituximab and biosimilar products in bioanalytical assays. ANTI HUMAN CD68 ANTIBODY, CLONE KP1 Figure A. FITC conjugated Mouse anti Human CD14 and Alexa Fluor 647 conjugated Mouse IgG1 isotype control ().Figure B. FITC conjugated Mouse anti Human CD14 and Alexa Fluor 647 conjugated Mouse anti Human CD68 (MCA5709A647).All experiments performed on Leucoperm fixed and permeabilized human peripheral blood gated on single cell mononuclearcells.
ANTI DOG MHC CLASS II MONOMORPHIC ANTIBODY, CLONE YKIX334 Rat anti Dog MHC Class II Monomorphic antibody, clone YKIX334.2 recognizes a monomorphic epitope on canine MHC Class II and was classified at the First Canine Leucocyte Antigen Workshop (Cobbold et al. 1994).The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections.In dogs, this is referred to as the dog leukocyte antigen (DLA) region. ANTI PIG CD203A ANTIBODY, CLONE PM18-7 Mouse anti Pig CD203a, clone PM18-7 recognizes porcine CD203a, originally clustered as SWC9 at the Second International Swine CD Workshop (Dominguez et al. 1998) and later identified as the porcine homologue of human ecto-nucleotidepyrophosphatase / phosphodiesterase 1 or ENPP1 (Petersen et al. 2007). Mouse anti Pig CD203a was originally reported to immunoprecipitate two bands, one of ~;205 RABBIT ANTI HUMAN SMAD3 ANTIBODY Rabbit anti Human SMAD3 antibody recognizes Mothers against decapentaplegic homolog 3 (SMAD3), a 50 kDa transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase, SMAD3 functions in cell proliferation, differentiation and development. Upon stimulation, SMAD3 oligomerizes with SMAD4 and translocates into the nucleus where it binds to DNA and transcription factors. ANTI PIG SLA CLASS II DR ANTIBODY, CLONE 2E9/13 Mouse anti Pig SLA Class II DR antibody, clone 2E9/13 recognizes SLA DR molecules which are expressed on all B cells, antigen presenting cells and on certain subsets of resting and activated T cells. Mouse anti Pig SLA Class II DR antibody, clone 289/13 reacts with lymphocytes from all outbred and miniature pigs so far tested, suggesting that it recognizes a monomorphic determinant of porcine ANTIBODY CONJUGATION KITS Bio-Rad offers two complimentary easy-to-use antibody conjugation kits, in a wide range of labels suitable for use in most applications. Readilink fluorophores have been optimized for flow cytometry whereas Lynx kits, in addition to common fluorophores include enzymes and biotin conjugations. CGAS-STING SIGNALING PATHWAY Overview of the cGAS-STING signaling pathway. The abnormal appearance of DNA in the cytoplasm triggers the activation of cGAS-cGAMP-STING signal. Induced production of type I interferons activates innate and adaptive immune responses. Antibodies including the KO validated antibodies to study the cGAS-STING signaling are presented. ANTIBODY WEBINARS AND VIDEOS Create mode – the default mode when you create a requisition and PunchOut to Bio-Rad. You can create and edit multiple shopping carts Edit mode – allows you to edit or modify an existing requisition (prior to submitting). You will be able to modify only the cart that you have PunchedOut to, and won't have access to any other carts MATERIAL SAFETY DATASHEETS Bio-Rad provides Material Safety Datasheets (MSDS) that comply with the latest regulations from the European Union*. Material Safety Datasheets (MSDS) for Bio-Rad products can be found by refering to the Health and Safety Information section of the specific product-related datasheets found on our website. Please contact antibody_tech_uk@bio-rad.com if you have difficulty finding the MSDSfor a
ALAMARBLUE PROTOCOLS Create mode – the default mode when you create a requisition and PunchOut to Bio-Rad. You can create and edit multiple shopping carts Edit mode – allows you to edit or modify an existing requisition (prior to submitting). You will be able to modify only the cart that you have PunchedOut to, and won't have access to any other carts ANTI PIG IGM ANTIBODY Published customer image: HRP conjugated Goat anti Pig IgM antibody (AAI48P) used to evaluate IgM levels in porcine serum samples by ELISA.Image caption: Experiment 1: Isotype profile of antibodies against S. suis serotype 7 in vaccinated sows and their piglets. Blood samples were collected 7 weeks and 1 week before farrowing from 25 vaccinated sows to analyze the immune response. ANTI MOUSE DECTIN-1 ANTIBODY, CLONE 2A11 Rat anti Mouse Dectin-1 antibody, clone 2A11 recognizes murine beta-glucan receptor, also known as Dectin-1.Dectin-1 is predominantly expressed by cells of the monocyte/macrophage and neutrophil lineages, but also at lower levels by dendritic cells and a subpopulation of T cells. As a major leucocyte receptor for beta-glucan this molecule may have a key role in the immunomodulatory effects of THE BUZZ AROUND STING AGONISTS This blog gives an overview of the history of the STimulator of Interferon Genes (STING) pathway and highlights why pharmaceutical companies are making a beeline to develop STING agonists. ANTI HUMAN CD3 ANTIBODY, CLONE CD3-12 Rat anti Human CD3, clone CD3-12 raised against a peptide representing an invariant cytoplasmic sequence within the CD3ε chain recognizes human CD3ε. CD3 is a multimeric protein complex composed of four distinct polypeptide chains (ε, γ, δ, ζ) that assemble and ANTI HUMAN CD4 ANTIBODY, CLONE RPA-T4 Mouse anti human CD4 antibody, clone RPA-T4 recognizes human CD4, a 55 kDa cell surface glycoprotein that is primarily expressed on a subpopulation of T lymphocytes, on peripheral blood monocytes and on tissue macrophages. Epitope mapping studies have shown that antibodies, produced by clone RPA-T4, recognize an epitope within domain 1 of the extracellular region of the CD4 molecule.BIO-RAD ANTIBODIES
Immunology. Our immunology portfolio featuring highly cited antibodies, kits, reagents, standards and controls for studying innate and adaptive immune responses. Antigen Density for Human and Murine Surface Markers. When building multicolor flow cytometry panels, the importance of antigen density is always highlighted. SPYTAG & SPYCATCHER PRODUCTS A MORE SUSTAINABLE SOLUTION FOR PRODUCT DATASHEETS A more sustainable solution for product datasheets. Watch the tutorial. From March 15, 2021, Bio-Rad no longer supplies printed datasheets with orders for products available from bio-rad-antibodies.com*. Each year, we send out tens of thousands of sheets of paper, many of which are destined to become waste, and so to reduce our impact on the CONTROLS FOR FLOW CYTOMETRY THE HISTORY BEHIND THE DISCOVERY OF RAPAMYCIN MATERIAL SAFETY DATASHEETS Material Safety Datasheets (MSDS) for Bio-Rad products can be found by refering to the Health and Safety Information section of the specific product-related datasheets found on our website. Please contact antibody_tech_uk@bio-rad.com if you have difficulty finding the MSDS for a particular product.WHAT IS ALAMARBLUE?
Overview. alamarBlue is a cell viability assay reagent which contains the cell permeable, non-toxic, and weakly fluorescent blue indicator dye called resazurin. This is a trusted and established reagent which has been available since 1993. alamarBlue is a useful non-toxic alternative to the commonly used MTT cell viability assay (3- (4,5 STARBRIGHT DYES FOR FLOW CYTOMETRY Fig. 2. StarBright Violet 515 Dye compared to other flow cytometry fluorophores. A, mouse peripheral blood was stained with CD4 PE or CD4 StarBright Violet 515 Dye (MCA2691SBV515). B, human peripheral blood was stained with CD4 conjugated to Amethyst Orange (), Brilliant Violet 510, and StarBright Violet 515 (MCA1267SBV515) Dye.The stain index for each is shown for all three dyes. ALAMARBLUE PROTOCOLS alamarBlue has been extensively referenced and used in a wide range of research areas. Below is a list of protocols including quality control, experimental optimization, general methods, examples, and calculations for using alamarBlue. Detailed protocols for use of alamarBlue: QC test for alamarBlue. Method to determine LD50 usingalamarBlue.
MACROPHAGES & THEIR MARKERSBIO-RAD ANTIBODIES
Immunology. Our immunology portfolio featuring highly cited antibodies, kits, reagents, standards and controls for studying innate and adaptive immune responses. Antigen Density for Human and Murine Surface Markers. When building multicolor flow cytometry panels, the importance of antigen density is always highlighted. SPYTAG & SPYCATCHER PRODUCTS A MORE SUSTAINABLE SOLUTION FOR PRODUCT DATASHEETS A more sustainable solution for product datasheets. Watch the tutorial. From March 15, 2021, Bio-Rad no longer supplies printed datasheets with orders for products available from bio-rad-antibodies.com*. Each year, we send out tens of thousands of sheets of paper, many of which are destined to become waste, and so to reduce our impact on the CONTROLS FOR FLOW CYTOMETRY THE HISTORY BEHIND THE DISCOVERY OF RAPAMYCIN MATERIAL SAFETY DATASHEETS Material Safety Datasheets (MSDS) for Bio-Rad products can be found by refering to the Health and Safety Information section of the specific product-related datasheets found on our website. Please contact antibody_tech_uk@bio-rad.com if you have difficulty finding the MSDS for a particular product.WHAT IS ALAMARBLUE?
Overview. alamarBlue is a cell viability assay reagent which contains the cell permeable, non-toxic, and weakly fluorescent blue indicator dye called resazurin. This is a trusted and established reagent which has been available since 1993. alamarBlue is a useful non-toxic alternative to the commonly used MTT cell viability assay (3- (4,5 STARBRIGHT DYES FOR FLOW CYTOMETRY Fig. 2. StarBright Violet 515 Dye compared to other flow cytometry fluorophores. A, mouse peripheral blood was stained with CD4 PE or CD4 StarBright Violet 515 Dye (MCA2691SBV515). B, human peripheral blood was stained with CD4 conjugated to Amethyst Orange (), Brilliant Violet 510, and StarBright Violet 515 (MCA1267SBV515) Dye.The stain index for each is shown for all three dyes. ALAMARBLUE PROTOCOLS alamarBlue has been extensively referenced and used in a wide range of research areas. Below is a list of protocols including quality control, experimental optimization, general methods, examples, and calculations for using alamarBlue. Detailed protocols for use of alamarBlue: QC test for alamarBlue. Method to determine LD50 usingalamarBlue.
MACROPHAGES & THEIR MARKERS ANTIBODY CONJUGATION KITS Human PBL stained with CD3 and CD20 conjugated to RPE and DyLight488. Bio-Rad offers two complimentary easy-to-use antibody conjugation kits, in a wide range of labels suitable for use in most applications. Readilink fluorophores have been optimized for flow cytometry whereas Lynx kits, in addition to common fluorophores include enzymes andINFLAMMATION
Accordingly, NF-κB also plays a role in the adaptive immune system, regulating the activation, differentiation, and function of inflammatory T cells (Liu et al. 2017). Bio-Rad offers a range of antibodies to various subunits of NF-κB, for use in applications such as ELISA, imaging, and western blotting.TYPES OF ELISA
High sensitivity - 2-5 times more sensitive than direct or indirect ELISA. Antibody optimization can be difficult - cross-reactivity may occur between the capture and detection antibodies. Needs a standardized ELISA kit or tested antibody pair. High specificity - two antibodies are involved in capture and detection.OPTICS & DETECTION
Lasers are the most commonly used light sources in flow cytometry. Lasers produce a single wavelength of light (a laser line) at a specific frequency. They are available at different wavelengths ranging from ultraviolet to far red and have a variable range of power levels (photon output/time typically specified in MACROPHAGES & THEIR MARKERS Macrophage Markers There are a large number of commonly used macrophage markers such as CD14, CD16, CD64, CD68, CD71 and CCR5; the exact marker to be used will be dependent upon the subset of macrophage and the conditions of their local environment.There are very few unique macrophage markers and often a number of markers will be required to identify your cell type. IMMUNOPHENOTYPING OF B CELLS BY FLOW CYTOMETRY B cell subsets can be difficult to identify due to variability and low level expression of markers and the rarity of some populations. This guide to immunophenotyping of human B cells takes you through some of the common markers and gating strategies used to identify B cells by flow cytometry, with examples of data acquired on the ZE5™ Cell ANTI RITUXIMAB ANTIBODY, CLONE MB2A4 Spectraviewer. Rat Anti-Rituximab Antibody, clone MB2A4, is an anti-idiotypic antibody that recognizes the monoclonal antibody drug rituximab. The antibody can be used to measure the levels of rituximab and biosimilar products in bioanalytical assays. Rat Anti-Rituximab Antibody also recognizes ocrelizumab, a second-generation humanizedanti
DENDRITIC CELLS & THEIR MARKERS Create mode – the default mode when you create a requisition and PunchOut to Bio-Rad. You can create and edit multiple shopping carts Edit mode – allows you to edit or modify an existing requisition (prior to submitting). You will be able to modify only the cart that you have PunchedOut to, and won't have access to any other carts MOUSE POSTERS, GUIDES & ONLINE DATABASE Designed for quick and easy identification of target mouse CD and non-CD markers, alongside marker specific information and a listing of the antibodies available to these markers. Simplifying one step in the process of antibody selection. Markers identified for mouse myeloid cells, lymphocytes and stem cells. ANTI CD20 ANTIBODY, CLONE RM272 Rabbit anti CD20 antibody recognizes B-lymphocyte antigen CD20, also known as Bp35 and MS4A1. CD20 is a non-glycosylated protein expressed on the surface of B lymphocytes and SPYTAG & SPYCATCHER PRODUCTS MATERIAL SAFETY DATASHEETS Material Safety Datasheets (MSDS) for Bio-Rad products can be found by refering to the Health and Safety Information section of the specific product-related datasheets found on our website. Please contact antibody_tech_uk@bio-rad.com if you have difficulty finding the MSDS for a particular product. THE HISTORY BEHIND THE DISCOVERY OF RAPAMYCIN ANTI RITUXIMAB ANTIBODY, CLONE MB2A4 Spectraviewer. Rat Anti-Rituximab Antibody, clone MB2A4, is an anti-idiotypic antibody that recognizes the monoclonal antibody drug rituximab. The antibody can be used to measure the levels of rituximab and biosimilar products in bioanalytical assays. Rat Anti-Rituximab Antibody also recognizes ocrelizumab, a second-generation humanizedanti
ANTI HUMAN CD3 ANTIBODY, CLONE CD3-12 Rat anti Human CD3, clone CD3-12 raised against a peptide representing an invariant cytoplasmic sequence within the CD3ε chain recognizes human CD3ε. CD3 is a multimeric protein complex composed of four distinct polypeptide chains (ε, γ, δ, ζ) that assemble and RABBIT ANTI HUMAN SMAD3 ANTIBODY Rabbit anti Human SMAD3 antibody recognizes Mothers against decapentaplegic homolog 3 (SMAD3), a 50 kDa transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase, SMAD3 functions in cell proliferation, differentiation and development. Upon stimulation, SMAD3 oligomerizes with SMAD4 and translocates into the nucleus where it binds to DNA and transcription factors. ALAMARBLUE PROTOCOLS alamarBlue has been extensively referenced and used in a wide range of research areas. Below is a list of protocols including quality control, experimental optimization, general methods, examples, and calculations for using alamarBlue. Detailed protocols for use of alamarBlue: QC test for alamarBlue. Method to determine LD50 usingalamarBlue.
ANTI P53 (AA20-25) ANTIBODY, CLONE DO-7 Mouse anti p53 antibody, clone DO-7 recognizes the human 53 kDa p53 tumour suppressor protein, also known as Cellular tumor antigen p53 or Antigen NY-CO-13, encoded by the TP53 gene. p53 is a 393 amino acid protein with an N-terminal transactivation domain, followed by a proline-rich region and a DNA binding domain in the central core region. The C-terminal region contains a tetramirization ANTI MOUSE DECTIN-1 ANTIBODY, CLONE 2A11 Rat anti Mouse Dectin-1 antibody, clone 2A11 recognizes murine beta-glucan receptor, also known as Dectin-1.Dectin-1 is predominantly expressed by cells of the monocyte/macrophage and neutrophil lineages, but also at lower levels by dendritic cells and a subpopulation of T cells. As a major leucocyte receptor for beta-glucan this molecule may have a key role in the immunomodulatory effects of INTRACELLULAR STAINING CONTROLS Because intracellular staining requires fixation and permeabilization which can affect antigen detection, autofluorescence, fluorophore brightness and cell morphology, other controls are necessary. These can include biological controls, such as stimulation/blocking experiments and negative/positive controls, which are described inmore detail
SPYTAG & SPYCATCHER PRODUCTS MATERIAL SAFETY DATASHEETS Material Safety Datasheets (MSDS) for Bio-Rad products can be found by refering to the Health and Safety Information section of the specific product-related datasheets found on our website. Please contact antibody_tech_uk@bio-rad.com if you have difficulty finding the MSDS for a particular product. THE HISTORY BEHIND THE DISCOVERY OF RAPAMYCIN ANTI RITUXIMAB ANTIBODY, CLONE MB2A4 Spectraviewer. Rat Anti-Rituximab Antibody, clone MB2A4, is an anti-idiotypic antibody that recognizes the monoclonal antibody drug rituximab. The antibody can be used to measure the levels of rituximab and biosimilar products in bioanalytical assays. Rat Anti-Rituximab Antibody also recognizes ocrelizumab, a second-generation humanizedanti
ANTI HUMAN CD3 ANTIBODY, CLONE CD3-12 Rat anti Human CD3, clone CD3-12 raised against a peptide representing an invariant cytoplasmic sequence within the CD3ε chain recognizes human CD3ε. CD3 is a multimeric protein complex composed of four distinct polypeptide chains (ε, γ, δ, ζ) that assemble and RABBIT ANTI HUMAN SMAD3 ANTIBODY Rabbit anti Human SMAD3 antibody recognizes Mothers against decapentaplegic homolog 3 (SMAD3), a 50 kDa transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase, SMAD3 functions in cell proliferation, differentiation and development. Upon stimulation, SMAD3 oligomerizes with SMAD4 and translocates into the nucleus where it binds to DNA and transcription factors. ALAMARBLUE PROTOCOLS alamarBlue has been extensively referenced and used in a wide range of research areas. Below is a list of protocols including quality control, experimental optimization, general methods, examples, and calculations for using alamarBlue. Detailed protocols for use of alamarBlue: QC test for alamarBlue. Method to determine LD50 usingalamarBlue.
ANTI P53 (AA20-25) ANTIBODY, CLONE DO-7 Mouse anti p53 antibody, clone DO-7 recognizes the human 53 kDa p53 tumour suppressor protein, also known as Cellular tumor antigen p53 or Antigen NY-CO-13, encoded by the TP53 gene. p53 is a 393 amino acid protein with an N-terminal transactivation domain, followed by a proline-rich region and a DNA binding domain in the central core region. The C-terminal region contains a tetramirization ANTI MOUSE DECTIN-1 ANTIBODY, CLONE 2A11 Rat anti Mouse Dectin-1 antibody, clone 2A11 recognizes murine beta-glucan receptor, also known as Dectin-1.Dectin-1 is predominantly expressed by cells of the monocyte/macrophage and neutrophil lineages, but also at lower levels by dendritic cells and a subpopulation of T cells. As a major leucocyte receptor for beta-glucan this molecule may have a key role in the immunomodulatory effects of INTRACELLULAR STAINING CONTROLS Because intracellular staining requires fixation and permeabilization which can affect antigen detection, autofluorescence, fluorophore brightness and cell morphology, other controls are necessary. These can include biological controls, such as stimulation/blocking experiments and negative/positive controls, which are described inmore detail
ANTIBODY CONJUGATION KITS Human PBL stained with CD3 and CD20 conjugated to RPE and DyLight488. Bio-Rad offers two complimentary easy-to-use antibody conjugation kits, in a wide range of labels suitable for use in most applications. Readilink fluorophores have been optimized for flow cytometry whereas Lynx kits, in addition to common fluorophores include enzymes and THE HISTORY BEHIND THE DISCOVERY OF RAPAMYCIN The HisTORy behind the Discovery of Rapamycin. 02 March, 2020. The fascinating story behind the discovery of mTOR signaling began during a scientific expedition to discover new antimicrobial agents. This blog tells the story of rapamycin’s discovery and highlights the important role that basic research plays in translational medicine. PORCINE CD MARKER ANTIBODIES s. Our extensive range of porcine cell surface marker antibodies includes products for epithelial cell, T and B cell, monocyte, macrophage and dendritic cell research. Anti porcine CD3, CD4a, CD8a, CD8b and CD25 for porcine T cell research. Antibodies for porcine dendritic cell research against CD163, CD172a (SIRP-alpha), SLA ClassII DQ and DR.
IMMUNOPHENOTYPING OF B CELLS BY FLOW CYTOMETRY B cell subsets can be difficult to identify due to variability and low level expression of markers and the rarity of some populations. This guide to immunophenotyping of human B cells takes you through some of the common markers and gating strategies used to identify B cells by flow cytometry, with examples of data acquired on the ZE5™ Cell ANTI PIG IGM ANTIBODY Published customer image: HRP conjugated Goat anti Pig IgM antibody (AAI48P) used to evaluate IgM levels in porcine serum samples by ELISA.Image caption: Experiment 1: Isotype profile of antibodies against S. suis serotype 7 in vaccinated sows and their piglets. Blood samples were collected 7 weeks and 1 week before farrowing from 25 vaccinated sows to analyze the immune response. ANTI RAT CD163 ANTIBODY, CLONE ED2 Mouse anti Rat CD163, clone ED2 recognizes the rat ED2 cell surface glycoprotein (Dijkstra et al. 1985).A 175 kDa molecule also known as rat CD163, a member of the group B scavenger receptor cysteine-rich (SRCR) family and an erythroblast adhesion receptor (Fabriek et al. 2007).Mouse anti rat CD163, clone ED2 was shown to detect approximately 50% of peritoneal macrophages, a subset of splenic STARBRIGHT VIOLET 440 DYE Overview Part of the new StarBright Dye range, StarBright Violet 440 (SBV440) Dye is a perfect replacement for fluorophores that have emission maxima between 430 and 480 nm, this includes dyes such as Pacific Blue, V450, vioBlue, and Alexa 405.Excitable by the violet 405 nm laser and emitting at 436 nm, it can be detected using the 460/22 filter on the ZE5 Cell Analyzer or a similar filter on ANTI CD20 ANTIBODY, CLONE RM272 Rabbit anti CD20 antibody recognizes B-lymphocyte antigen CD20, also known as Bp35 and MS4A1. CD20 is a non-glycosylated protein expressed on the surface of B lymphocytes and ANTI MOUSE CD68 ANTIBODY, CLONE FA-11 Rat anti Mouse CD68:Alexa Fluor® 700. CD68 antibody, clone FA-11 recognizes mouse macrosialin which is a homolog of human CD68, which is classified as a unique scavenger receptor (ScR) family member. CD68 is considered a pan macrophage marker expressed on the intracellularlysosomes of
DOUBLET DISCRIMINATION Doublet discrimination. Doublets (red) can be distinguished from single cells (green) by plotting FSC height vs FCS area. Doublets have increased area whilst similar height to single cells. Removal of doublets is also crucial in cell cycle analysis. It is important to distinguish between single cells that have double the amount of DNAand
ANTI-USTEKINUMAB ANTIBODIES (STELARA) ANTIBODY WEBINARS AND VIDEOS This webinar summarises a feasibility study to discover whether recombinant anti-peptide antibodies generated using the HuCAL antibody library and phage display technology are suitable for immunoaffinity enrichment of peptides coupled to targeted MRM. Speaker: Dr Christian Frisch, R&D Manager, Bio-Rad. CONTROLS FOR FLOW CYTOMETRYWHAT IS ALAMARBLUE?
Overview. alamarBlue is a cell viability assay reagent which contains the cell permeable, non-toxic, and weakly fluorescent blue indicator dye called resazurin. This is a trusted and established reagent which has been available since 1993. alamarBlue is a useful non-toxic alternative to the commonly used MTT cell viability assay (3- (4,5 NF-ΚB SIGNALING PATHWAY Cancer Pathway Posters. Compiling key proteins involved in key signaling pathways: EGF R, mTOR, NF-kB, and PI3K/AKT. The transcription factor Nuclear Factor-Kappa B (NF-κB) is a major regulator of inflammation and controls many aspects of innate and adaptive immune responses. However, constitutive activation of NF-κBis associated with
MATERIAL SAFETY DATASHEETS Material Safety Datasheets (MSDS) for Bio-Rad products can be found by refering to the Health and Safety Information section of the specific product-related datasheets found on our website. Please contact antibody_tech_uk@bio-rad.com if you have difficulty finding the MSDS for a particular product.TYPES OF ELISA
WHY USE A WESTERN BLOT? IMMUNOBLOT Lane 1: Target protein band at the expected molecular weight .Lanes 2 & 3: Common alterations that modify the molecular weight Western blots are in wide use across a broad range of scientific and clinical disciplines . Their ability to clearly show the presence of a specific protein both by size and through the binding of an antibody makes them well-suited for evaluating levels of protein DOUBLET DISCRIMINATION Create mode – the default mode when you create a requisition and PunchOut to Bio-Rad. You can create and edit multiple shopping carts Edit mode – allows you to edit or modify an existing requisition (prior to submitting). You will be able to modify only the cart that you have PunchedOut to, and won't have access to any other carts ANTI P53 (AA20-25) ANTIBODY, CLONE DO-7 Mouse anti p53 antibody, clone DO-7 recognizes the human 53 kDa p53 tumour suppressor protein, also known as Cellular tumor antigen p53 or Antigen NY-CO-13, encoded by the TP53 gene. p53 is a 393 amino acid protein with an N-terminal transactivation domain, followed by a proline-rich region and a DNA binding domain in the central core region. The C-terminal region contains a tetramirization ANTI-USTEKINUMAB ANTIBODIES (STELARA) ANTIBODY WEBINARS AND VIDEOS This webinar summarises a feasibility study to discover whether recombinant anti-peptide antibodies generated using the HuCAL antibody library and phage display technology are suitable for immunoaffinity enrichment of peptides coupled to targeted MRM. Speaker: Dr Christian Frisch, R&D Manager, Bio-Rad. CONTROLS FOR FLOW CYTOMETRYWHAT IS ALAMARBLUE?
Overview. alamarBlue is a cell viability assay reagent which contains the cell permeable, non-toxic, and weakly fluorescent blue indicator dye called resazurin. This is a trusted and established reagent which has been available since 1993. alamarBlue is a useful non-toxic alternative to the commonly used MTT cell viability assay (3- (4,5 NF-ΚB SIGNALING PATHWAY Cancer Pathway Posters. Compiling key proteins involved in key signaling pathways: EGF R, mTOR, NF-kB, and PI3K/AKT. The transcription factor Nuclear Factor-Kappa B (NF-κB) is a major regulator of inflammation and controls many aspects of innate and adaptive immune responses. However, constitutive activation of NF-κBis associated with
MATERIAL SAFETY DATASHEETS Material Safety Datasheets (MSDS) for Bio-Rad products can be found by refering to the Health and Safety Information section of the specific product-related datasheets found on our website. Please contact antibody_tech_uk@bio-rad.com if you have difficulty finding the MSDS for a particular product.TYPES OF ELISA
WHY USE A WESTERN BLOT? IMMUNOBLOT Lane 1: Target protein band at the expected molecular weight .Lanes 2 & 3: Common alterations that modify the molecular weight Western blots are in wide use across a broad range of scientific and clinical disciplines . Their ability to clearly show the presence of a specific protein both by size and through the binding of an antibody makes them well-suited for evaluating levels of protein DOUBLET DISCRIMINATION Create mode – the default mode when you create a requisition and PunchOut to Bio-Rad. You can create and edit multiple shopping carts Edit mode – allows you to edit or modify an existing requisition (prior to submitting). You will be able to modify only the cart that you have PunchedOut to, and won't have access to any other carts ANTI P53 (AA20-25) ANTIBODY, CLONE DO-7 Mouse anti p53 antibody, clone DO-7 recognizes the human 53 kDa p53 tumour suppressor protein, also known as Cellular tumor antigen p53 or Antigen NY-CO-13, encoded by the TP53 gene. p53 is a 393 amino acid protein with an N-terminal transactivation domain, followed by a proline-rich region and a DNA binding domain in the central core region. The C-terminal region contains a tetramirization TECHNICAL SUPPORT, FAQS & LICENCE QUERIES Create mode – the default mode when you create a requisition and PunchOut to Bio-Rad. You can create and edit multiple shopping carts Edit mode – allows you to edit or modify an existing requisition (prior to submitting). You will be able to modify only the cart that you have PunchedOut to, and won't have access to any other carts MATERIAL SAFETY DATASHEETS Material Safety Datasheets (MSDS) for Bio-Rad products can be found by refering to the Health and Safety Information section of the specific product-related datasheets found on our website. Please contact antibody_tech_uk@bio-rad.com if you have difficulty finding the MSDS for a particular product. HUCAL TECHNOLOGY EXPLAINED HuCAL PLATINUM ® is a synthetic library of 45 billion fully human antibodies in Fab format . The HuCAL PLATINUM phage library contains more than 45 billion functional human antibody genes in a Fab format. It has been designed to cover the human antibody diversity, to be flexible for further engineering and to yield antibodies with highexpression titers.
STARBRIGHT DYES FOR FLOW CYTOMETRY Fig. 2. StarBright Violet 515 Dye compared to other flow cytometry fluorophores. A, mouse peripheral blood was stained with CD4 PE or CD4 StarBright Violet 515 Dye (MCA2691SBV515). B, human peripheral blood was stained with CD4 conjugated to Amethyst Orange (), Brilliant Violet 510, and StarBright Violet 515 (MCA1267SBV515) Dye.The stain index for each is shown for all three dyes. ANTI HUMAN IGG ANTIBODY This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C. Avoid repeated freezing and thawing as this may denature the antibody. WHY USE A WESTERN BLOT? IMMUNOBLOT Lane 1: Target protein band at the expected molecular weight .Lanes 2 & 3: Common alterations that modify the molecular weight Western blots are in wide use across a broad range of scientific and clinical disciplines . Their ability to clearly show the presence of a specific protein both by size and through the binding of an antibody makes them well-suited for evaluating levels of protein ANTI RITUXIMAB ANTIBODY, CLONE MB2A4 Spectraviewer. Rat Anti-Rituximab Antibody, clone MB2A4, is an anti-idiotypic antibody that recognizes the monoclonal antibody drug rituximab. The antibody can be used to measure the levels of rituximab and biosimilar products in bioanalytical assays. Rat Anti-Rituximab Antibody also recognizes ocrelizumab, a second-generation humanizedanti
PGP 9.5 ANTIBODY (PROTEIN GENE PRODUCT 9.5) Protein gene product (PGP) 9.5, also referred to as ubiquitin carboxy-terminal hydrolase L1 (UCHL1), is a deubiquitinating enzyme with both hydrolase and ligase activity. Its function is to bind and stabilize monoubiquitin, hydrolyze polyubiquitin, and carry out additional ubiquitinylation of ubiquitinylated proteins (Osaka et al.2003).
MACROPHAGES & THEIR MARKERS Macrophage Markers. There are a large number of commonly used macrophage markers such as CD14, CD16, CD64, CD68, CD71 and CCR5; the exact marker to be used will be dependent upon the subset of macrophage and the conditions of their local environment. There are very few unique macrophage markers and often a number of markers willbe required to
DECIDING WHICH ELISA TECHNIQUE IS RIGHT FOR YOU Deciding which ELISA technique is right for you. The enzyme linked immunosorbent assay (ELISA) was first described by Engvall and Perlmann (1971), and used initially for the detection of immunoglobulin G. This immunoassay was a welcomed change from the then common radioimmunoassay, which utilized radioactively labeled antibodies and antigens. WELCOME TO BIO-RAD.COM Please select your location to view the products, information, and services available, including news, promotions and events.. Bringing you a world of innovation...THE AMERICAS
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