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U-937 | ATCC
Studies since 1979 have shown that U-937 cells can be induced to terminal monocytic differentiation by supernatants from human mixed lymphocyte cultures, The cells are negative for immunoglobulin production and Epstein-Barr virus expression. The cells express the Fas antigen, and are sensitive to TNF and anti-Fas antibodies. In 1994, PCR and cytogenetic analyses showed that a number of stocksCHO-K1 | ATCC
Certificate of Analysis Download. To download a certificate of analysis for CHO-K1 ( CCL-61 ), enter the lot number exactly as it appears on your product label or packing slip. Lot number. Get Certificate of Analysis. Certificate of Analysis Request. The certificate of analysis for that lot of CHO-K1 ( CCL-61) is not currently available online.THP-1 | ATCC
The lot specific information (number of cells and expected viability) from the Certificate of Analysis (COA) should be used to calculate the volume of medium necessary to start the THP-1 (ATCC TIB-202) cell culture at the recommended seeding density of 3 XNCI-H460
To download a certificate of origin for NCI-H460 ( HTB-177 ), enter the lot number exactly as it appears on your product label or packing slip. The certificate of origin for that lot of NCI-H460 ( HTB-177) is not currently available online. Complete this form to request this certificate of origin.MCF7 | ATCC
The MCF7 line retains several characteristics of differentiated mammary epithelium including ability to process estradiol via cytoplasmic estrogen receptors and the capability of forming domes. The cells express the WNT7B oncogene.RefHuguet EL, et al. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue.HMC3 | ATCC
Resting HMC3 cells were strongly positive for the microglia/macrophage marker IBA1, positive for the endotoxin receptor CD14, but negative for the astrocyte marker GFAP. Markers of activated microglia, namely MHCII, CD68 and CD11b were negative in resting HMC3 cells, but upregulated after activation by IFN-gamma (10 ng/ml, 24 h).A-431 | ATCC
Documentation. To download a certificate of analysis for A-431 ( CRL-1555 ), enter the lot number exactly as it appears on your product label or packing slip. The certificate of analysis for that lot of A-431 ( CRL-1555) is not currently available online. Complete this form to request this certificate of analysis.PANC-1 | ATCC
Remove medium, and rinse with 0.25% trypsin, 0.53mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. EAGLE'S MINIMUM ESSENTIAL MEDIUM (EMEM) Eagle's Minimum Essential Medium (EMEM) 30-2003 ™. Eagle's minimum essential medium modified to contain Earle's Balanced Salt Solution, non-essential amino acids, 2 mM L-glutamine, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate. ATCC: THE GLOBAL BIORESOURCE CENTER ATCC is a nonprofit organization that collects, stores, and distributes standard reference microorganisms, cell lines, and other materials for research and development.U-937 | ATCC
Studies since 1979 have shown that U-937 cells can be induced to terminal monocytic differentiation by supernatants from human mixed lymphocyte cultures, The cells are negative for immunoglobulin production and Epstein-Barr virus expression. The cells express the Fas antigen, and are sensitive to TNF and anti-Fas antibodies. In 1994, PCR and cytogenetic analyses showed that a number of stocksCHO-K1 | ATCC
Certificate of Analysis Download. To download a certificate of analysis for CHO-K1 ( CCL-61 ), enter the lot number exactly as it appears on your product label or packing slip. Lot number. Get Certificate of Analysis. Certificate of Analysis Request. The certificate of analysis for that lot of CHO-K1 ( CCL-61) is not currently available online.THP-1 | ATCC
The lot specific information (number of cells and expected viability) from the Certificate of Analysis (COA) should be used to calculate the volume of medium necessary to start the THP-1 (ATCC TIB-202) cell culture at the recommended seeding density of 3 XNCI-H460
To download a certificate of origin for NCI-H460 ( HTB-177 ), enter the lot number exactly as it appears on your product label or packing slip. The certificate of origin for that lot of NCI-H460 ( HTB-177) is not currently available online. Complete this form to request this certificate of origin.MCF7 | ATCC
The MCF7 line retains several characteristics of differentiated mammary epithelium including ability to process estradiol via cytoplasmic estrogen receptors and the capability of forming domes. The cells express the WNT7B oncogene.RefHuguet EL, et al. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue.HMC3 | ATCC
Resting HMC3 cells were strongly positive for the microglia/macrophage marker IBA1, positive for the endotoxin receptor CD14, but negative for the astrocyte marker GFAP. Markers of activated microglia, namely MHCII, CD68 and CD11b were negative in resting HMC3 cells, but upregulated after activation by IFN-gamma (10 ng/ml, 24 h).A-431 | ATCC
Documentation. To download a certificate of analysis for A-431 ( CRL-1555 ), enter the lot number exactly as it appears on your product label or packing slip. The certificate of analysis for that lot of A-431 ( CRL-1555) is not currently available online. Complete this form to request this certificate of analysis.PANC-1 | ATCC
Remove medium, and rinse with 0.25% trypsin, 0.53mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. EAGLE'S MINIMUM ESSENTIAL MEDIUM (EMEM) Eagle's Minimum Essential Medium (EMEM) 30-2003 ™. Eagle's minimum essential medium modified to contain Earle's Balanced Salt Solution, non-essential amino acids, 2 mM L-glutamine, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate.MCF 10A | ATCC
The MCF 10A cell line is a non-tumorigenic epithelial cell line.The cells are positive for epithelial sialomucins, cytokeratins and milk fat globule antigen.They exhibit three dimensional growth in collagen, and form domes in confluent cultures.Thus far, the cells have shown no signs of terminal differentiation or senescence.The line is responsive to insulin, glucocorticoids, cholera ATCC: THE GLOBAL BIORESOURCE CENTER Place the ampule in a water bath at 37°C and agitate until the last bit of ice just thawed. Transfer the contents to a culture vessel with 10 volumes of appropriate medium. This ten-fold dilution will lower the concentration of the cryoprotective agent, such as DMSO, that could harm the cells.HELA | ATCC
Four typical HeLa marker chromosomes have been reported in the literature. HeLa Marker Chromosomes: One copy of Ml, one copy of M2, four-five copies of M3, and two copies of M4 as revealed by G-banding patterns. M1 is a rearranged long arm and centromere of chromosome 1DLD-1 | ATCC
This cell line is one of four colorectal adenocarcinoma cell lines that have been identified as being derived from a single individual. DNA profiling studies have shown that DLD-1 (ATCC CCL-221), HCT-15 (ATCC CCL-225), HCT-8 (ATCC CCL-244) and HRT-18G (ATCC CRL-11663) share a single profile. DNA fingerprinting and cytogenetic analyses performed at ATCC and elsewhere show the line is293
Although an earlier report suggested that the cells contained Adenovirus 5 DNA from both the right and left ends of the viral genome , it is now clear that only left end sequences are present.The cells express an unusual cell surface receptor for vitronectin composed of the integrin beta-1 subunit and the vitronectin receptor alpha-v subunit.The Ad5 insert was cloned andsequenced
CT26.WT | ATCC
CT26.WT was stably transduced with the retroviral vector LXSN that contains the lacZ gene encoding the model tumor associated antigen (TAA), beta-galactosidase (beta-gal) to obtain the lethal subclone CT26.CL25 (ATCC CRL-2639). The growth rate and lethality of CT26.CL25 and CT26.WT is virtually identical despite the expression by CT26.CL25 of the model TAA, beta-galactosidase, in normal mice.HTERT RPE-1
The hTERT-immortalized retinal pigment epithelial cell line, hTERT RPE-1, was derived by transfecting the RPE-340 cell line with the pGRN145 hTERT-expressing plasmid ( ATCC MBA-141 ). Cells were cultured in medium containing hygromycin B until stable clones were selected.
BT-474 | ATCC
Documentation. To download a certificate of analysis for BT-474 ( HTB-20 ), enter the lot number exactly as it appears on your product label or packing slip. The certificate of analysis for that lot of BT-474 ( HTB-20) is not currently available online. Complete this form to request this certificate of analysis.SH-SY5Y | ATCC
SH-SY5Y cells have a reported saturation density greater than 1 X 106 cells/cm2. They are reported to exhibit moderate levels of dopamine beta hydroxylase activity. RefBiedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. PubMed: 29704ATCC.ORG
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