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PROTOCOLS
18th International Xenopus Conference. The 18th International Xenopus Conference will be held virtually with an in-person event. August15-19, 2021.
XENOPUS LAEVIS STAGE SERIES: COMPLETE Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari. INTRODUCTION TO XENOPUS Introduction to Xenopus, the frog model. Xenopus are an invaluable tool to study vertebrate embryology and development, basic cell and molecular biology, genomics, neurobiology and toxicology and to model human diseases. Studying model organisms, such as Xenopus, allows us to decipher how regulatory and interactions networks direct embryonicXENOPUS FATE MAPS
stage 8. stage 10.5. Fate mapping data and images based upon the work of Moody 1987a , 1987b and Bauer et al. 1994. Technical notes. Fate maps: reverse. Move cursor over cell/tissue type and the blastula map will appear. Mapping data based upon Moody 1987 . and Mills et al. 1999. receives major contribution from red blastomeres. XENOPUS DEVELOPMENT STAGESRetrieved from
"http://wiki.xenbase.org/xenwiki/index.php?title=Xenopus_development_stages&oldid=46326" SHIBATA Y ET AL. (2021), THYROID HORMONE RECEPTOR IS Abstract. Vertebrate postembryonic development is regulated by thyroid hormone (T3). Of particular interest is anuran metamorphosis, which offers several unique advantages for studying the role of T3 and its two nuclear receptor genes, TRα and TRβ, during postembryonic development. We have recently generated TR double knockout (TRDKO)Xenopus
KANAI Y ET AL. (1994), THE HUMAN KIDNEY LOW AFFINITY NA+ Abstract The major reabsorptive mechanism for D-glucose in the kidney is known to involve a low affinity high capacity Na+/glucose cotransporter, which is located in the early proximal convoluted tubule segment S1, and which has a Na+ to glucose coupling ratio of 1:1. Here we provide the first molecular evidence for this renal D-glucose reabsorptive mechanism.ATF1 EXPRESSION
Proteins (38) Interactants (89) Wiki. XB-GENEPAGE-971676. Gene Symbol: atf1. Gene Name: activating transcription factor 1. Anatomy terms. Keller explant brain ectoderm egg head intestine oocyte skeletal muscle testis thymus upper blastopore lip whole organism.KARL KUNZELMANN
Dr. Karl Kunzelmann Position Professor of Physiology, Group Leader Research Description Role of anoctamins in renal tubules and polycystic kidney disease Role of CFRT in renal HCO3 secretion and salt absorption Role of TEM16A and TEM16F in cellular exocytosis Treatment of cystic fibrosis by SPENCE JR ET AL. (2011), DIRECTED DIFFERENTIATION OF HUMAN Abstract. Studies in embryonic development have guided successful efforts to direct the differentiation of human embryonic and induced pluripotent stem cells (PSCs) into specific organ cell types in vitro. For example, human PSCs have been differentiated into monolayer cultures of liver hepatocytes and pancreatic endocrine cells that have XENBASE HOMEBLASTGENOMESEXPRESSIONGENESANATOMY & DEVELOPMENTREAGENTS &PROTOCOLS
18th International Xenopus Conference. The 18th International Xenopus Conference will be held virtually with an in-person event. August15-19, 2021.
XENOPUS LAEVIS STAGE SERIES: COMPLETE Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari. INTRODUCTION TO XENOPUS Introduction to Xenopus, the frog model. Xenopus are an invaluable tool to study vertebrate embryology and development, basic cell and molecular biology, genomics, neurobiology and toxicology and to model human diseases. Studying model organisms, such as Xenopus, allows us to decipher how regulatory and interactions networks direct embryonicXENOPUS FATE MAPS
stage 8. stage 10.5. Fate mapping data and images based upon the work of Moody 1987a , 1987b and Bauer et al. 1994. Technical notes. Fate maps: reverse. Move cursor over cell/tissue type and the blastula map will appear. Mapping data based upon Moody 1987 . and Mills et al. 1999. receives major contribution from red blastomeres. XENOPUS DEVELOPMENT STAGESRetrieved from
"http://wiki.xenbase.org/xenwiki/index.php?title=Xenopus_development_stages&oldid=46326" SHIBATA Y ET AL. (2021), THYROID HORMONE RECEPTOR IS Abstract. Vertebrate postembryonic development is regulated by thyroid hormone (T3). Of particular interest is anuran metamorphosis, which offers several unique advantages for studying the role of T3 and its two nuclear receptor genes, TRα and TRβ, during postembryonic development. We have recently generated TR double knockout (TRDKO)Xenopus
KANAI Y ET AL. (1994), THE HUMAN KIDNEY LOW AFFINITY NA+ Abstract The major reabsorptive mechanism for D-glucose in the kidney is known to involve a low affinity high capacity Na+/glucose cotransporter, which is located in the early proximal convoluted tubule segment S1, and which has a Na+ to glucose coupling ratio of 1:1. Here we provide the first molecular evidence for this renal D-glucose reabsorptive mechanism.ATF1 EXPRESSION
Proteins (38) Interactants (89) Wiki. XB-GENEPAGE-971676. Gene Symbol: atf1. Gene Name: activating transcription factor 1. Anatomy terms. Keller explant brain ectoderm egg head intestine oocyte skeletal muscle testis thymus upper blastopore lip whole organism.KARL KUNZELMANN
Dr. Karl Kunzelmann Position Professor of Physiology, Group Leader Research Description Role of anoctamins in renal tubules and polycystic kidney disease Role of CFRT in renal HCO3 secretion and salt absorption Role of TEM16A and TEM16F in cellular exocytosis Treatment of cystic fibrosis by SPENCE JR ET AL. (2011), DIRECTED DIFFERENTIATION OF HUMAN Abstract. Studies in embryonic development have guided successful efforts to direct the differentiation of human embryonic and induced pluripotent stem cells (PSCs) into specific organ cell types in vitro. For example, human PSCs have been differentiated into monolayer cultures of liver hepatocytes and pancreatic endocrine cells that have GENE EXPRESSION SEARCH Gene Symbol Clone Name Affymetrix Id Search Synonyms Xenopus tropicalis laevis. BLAST Sequence to Find Gene Expression: Sequence, Accession or Gi. Evalue. Developmental Stages: All mature egg stage embryonic stage NF stage NF stage 1 cleavage stage NF stage 2- NF stage 2 (2-cell) NF stage 3 (4-cell) NF stage 4 (8-cell) NF stage 5(16-cell) NF
XENOPUS ANATOMY ONTOLOGY Xenopus Anatomy Ontology. The Xenopus Anatomy Ontology, aka the XAO, describes Xenopus anatomy and embryological development using a 'controlled vocabulary' of anatomy terms that are organized in an hierarchy with a graphical structure. Xenbase curators use XAO terms to describe gene expression, and the XAO is constantly being updated in response to the latest published Xenopus research. XENOPUS MOVIES OF DEVELOPMENT Synchronous development of Xenopus laevis embryos during early embryogenesis. Gastrulation follows the synchronous cleavage stages, and migrating involuted mesoderm can be seen underlying the prospective ectoderm cells. If playback doesn't begin shortly, try restarting your device. Videos you watch may be added to the TV'swatch history and
FTP DATA FILES
Xenbase data downloads provide access to genomes, database content, and more. Most files are in a tab delimited format. Click the "readme" link to view information on the files, including the header row for these files. To download a file, click on the corresponding link on the far right side. More files are available on our FTP site.XENOPUS SUPPLIERS
water quality monitoring systems. +1 617 966 9675. +1 508 763 3052. kedminster@electromechanica.com. Aquatic Enterprises, Inc. (USA) frog holding systems. +1 206 937 0392. +1 206 937 1099. info@aquaticenterprises.com. XENBASE ANATOMY ONTOLOGY: PAPERS FOR VEIN Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari. XENOPUS ATLAS: TISSUES: HEART 5A. Stage 46: cardiac troponin T (red) and fibrillin (green). This is the first of a series of dorsal to ventral stacks (5A - 5F). XENOPUS ANATOMY ONTOLOGY: SUMMARY FOR APOPTOTIC CELL Definition: "A cell that is in the process of programmed cell death.The process is characterized by morphological changes (e.g. blebbing, cell shrinkage, nuclear fragmentation, chromatin condensation, and chromosomal DNA fragmentation) ending in celldeath."
XENOPUS ANATOMY ONTOLOGY: SUMMARY FOR EYE Definition: "The organ of sight (visual apparatus), almost spherical in shape.At the end of development (NF stage 66) the eyes bulge out of their orbital capsules near the XENOPUS ANATOMY ONTOLOGY: SUMMARY FOR FOREBRAIN Definition: "The part of the brain that is the anterior of its three primary divisions and that includes the telencephalon anddiencephalon."
XENBASE HOMEBLASTGENOMESEXPRESSIONGENESANATOMY & DEVELOPMENTREAGENTS &PROTOCOLS
The Xenopus model organism knowledgebase. Xenbase is a web-accessible resource that integrates all the diverse biological, genomic, genotype and phenotype data available from Xenopus research. Learn more about Xenbase . Search Xenopus Papers and Books on XENOPUS LAEVIS STAGE SERIES: COMPLETE Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari. INTRODUCTION TO XENOPUS Introduction to Xenopus, the frog model. Xenopus are an invaluable tool to study vertebrate embryology and development, basic cell and molecular biology, genomics, neurobiology and toxicology and to model human diseases. Studying model organisms, such as Xenopus, allows us to decipher how regulatory and interactions networks direct embryonic GENE EXPRESSION SEARCH Gene Symbol Clone Name Affymetrix Id Search Synonyms Xenopus tropicalis laevis. BLAST Sequence to Find Gene Expression: Sequence, Accession or Gi. Evalue. Developmental Stages: All mature egg stage embryonic stage NF stage NF stage 1 cleavage stage NF stage 2- NF stage 2 (2-cell) NF stage 3 (4-cell) NF stage 4 (8-cell) NF stage 5(16-cell) NF
XENOPUS FATE MAPS
stage 8. stage 10.5. Fate mapping data and images based upon the work of Moody 1987a , 1987b and Bauer et al. 1994. Technical notes. Fate maps: reverse. Move cursor over cell/tissue type and the blastula map will appear. Mapping data based upon Moody 1987 . and Mills et al. 1999. receives major contribution from red blastomeres. XENOPUS ATLAS: TISSUES: HEART 5A. Stage 46: cardiac troponin T (red) and fibrillin (green). This is the first of a series of dorsal to ventral stacks (5A - 5F). XENOPUS DEVELOPMENT STAGESRetrieved from
"http://wiki.xenbase.org/xenwiki/index.php?title=Xenopus_development_stages&oldid=46326" KANAI Y ET AL. (1994), THE HUMAN KIDNEY LOW AFFINITY NA+ Abstract The major reabsorptive mechanism for D-glucose in the kidney is known to involve a low affinity high capacity Na+/glucose cotransporter, which is located in the early proximal convoluted tubule segment S1, and which has a Na+ to glucose coupling ratio of 1:1. Here we provide the first molecular evidence for this renal D-glucose reabsorptive mechanism.ATF1 EXPRESSION
Proteins (38) Interactants (89) Wiki. XB-GENEPAGE-971676. Gene Symbol: atf1. Gene Name: activating transcription factor 1. Anatomy terms. Keller explant brain ectoderm egg head intestine oocyte skeletal muscle testis thymus upper blastopore lip whole organism. IN SITU HYBRIDIZATION Add a small amount of water to ingredients, make a slurry, then dilute. Store at -20C. RNase A (Sigma R 5000) Dissolve 10mg/ml in TE (pH 7.8) and boil for 10 min before use. Store aliquots at -20C. RNase T1 (Sigma R8251) Dissolve 10,000 units/ ml (1000X) in 0.1 M XENBASE HOMEBLASTGENOMESEXPRESSIONGENESANATOMY & DEVELOPMENTREAGENTS &PROTOCOLS
The Xenopus model organism knowledgebase. Xenbase is a web-accessible resource that integrates all the diverse biological, genomic, genotype and phenotype data available from Xenopus research. Learn more about Xenbase . Search Xenopus Papers and Books on XENOPUS LAEVIS STAGE SERIES: COMPLETE Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari. INTRODUCTION TO XENOPUS Introduction to Xenopus, the frog model. Xenopus are an invaluable tool to study vertebrate embryology and development, basic cell and molecular biology, genomics, neurobiology and toxicology and to model human diseases. Studying model organisms, such as Xenopus, allows us to decipher how regulatory and interactions networks direct embryonic GENE EXPRESSION SEARCH Gene Symbol Clone Name Affymetrix Id Search Synonyms Xenopus tropicalis laevis. BLAST Sequence to Find Gene Expression: Sequence, Accession or Gi. Evalue. Developmental Stages: All mature egg stage embryonic stage NF stage NF stage 1 cleavage stage NF stage 2- NF stage 2 (2-cell) NF stage 3 (4-cell) NF stage 4 (8-cell) NF stage 5(16-cell) NF
XENOPUS FATE MAPS
stage 8. stage 10.5. Fate mapping data and images based upon the work of Moody 1987a , 1987b and Bauer et al. 1994. Technical notes. Fate maps: reverse. Move cursor over cell/tissue type and the blastula map will appear. Mapping data based upon Moody 1987 . and Mills et al. 1999. receives major contribution from red blastomeres. XENOPUS ATLAS: TISSUES: HEART 5A. Stage 46: cardiac troponin T (red) and fibrillin (green). This is the first of a series of dorsal to ventral stacks (5A - 5F). XENOPUS DEVELOPMENT STAGESRetrieved from
"http://wiki.xenbase.org/xenwiki/index.php?title=Xenopus_development_stages&oldid=46326" KANAI Y ET AL. (1994), THE HUMAN KIDNEY LOW AFFINITY NA+ Abstract The major reabsorptive mechanism for D-glucose in the kidney is known to involve a low affinity high capacity Na+/glucose cotransporter, which is located in the early proximal convoluted tubule segment S1, and which has a Na+ to glucose coupling ratio of 1:1. Here we provide the first molecular evidence for this renal D-glucose reabsorptive mechanism.ATF1 EXPRESSION
Proteins (38) Interactants (89) Wiki. XB-GENEPAGE-971676. Gene Symbol: atf1. Gene Name: activating transcription factor 1. Anatomy terms. Keller explant brain ectoderm egg head intestine oocyte skeletal muscle testis thymus upper blastopore lip whole organism. IN SITU HYBRIDIZATION Add a small amount of water to ingredients, make a slurry, then dilute. Store at -20C. RNase A (Sigma R 5000) Dissolve 10mg/ml in TE (pH 7.8) and boil for 10 min before use. Store aliquots at -20C. RNase T1 (Sigma R8251) Dissolve 10,000 units/ ml (1000X) in 0.1 M INTRODUCTION TO XENOPUS Introduction to Xenopus, the frog model. Xenopus are an invaluable tool to study vertebrate embryology and development, basic cell and molecular biology, genomics, neurobiology and toxicology and to model human diseases. Studying model organisms, such as Xenopus, allows us to decipher how regulatory and interactions networks direct embryonic GENE EXPRESSION SEARCH Gene Symbol Clone Name Affymetrix Id Search Synonyms Xenopus tropicalis laevis. BLAST Sequence to Find Gene Expression: Sequence, Accession or Gi. Evalue. Developmental Stages: All mature egg stage embryonic stage NF stage NF stage 1 cleavage stage NF stage 2- NF stage 2 (2-cell) NF stage 3 (4-cell) NF stage 4 (8-cell) NF stage 5(16-cell) NF
CITING XENBASE
Citing Xenbase. How to cite Xenbase. The NIH requests that NIH-funded resources be cited by authors who used these publicly funded resources. Similarly, the NIH and many major publishing houses, such as Nature Publishing Group, Elsevier, Wiley, and Company of Biologists request authors use RRID numbers to accurately identify the reagentsand
XENBASE
Indicate Lab of origin using a ILAR lab code, in superscript, following parentheses. When a sequence of the alternate Xenopus species is used i.e., a X. laevis line that carries a construct made with X. tropicalis sequence, indicate this within the parenthesesusing the 3
XENOPUS SUPPLIERS
water quality monitoring systems. +1 617 966 9675. +1 508 763 3052. kedminster@electromechanica.com. Aquatic Enterprises, Inc. (USA) frog holding systems. +1 206 937 0392. +1 206 937 1099. info@aquaticenterprises.com.FTP DATA FILES
Xenbase data downloads provide access to genomes, database content, and more. Most files are in a tab delimited format. Click the "readme" link to view information on the files, including the header row for these files. To download a file, click on the corresponding link on the far right side. More files are available on our FTP site. XENOPUS DEVELOPMENT TIMES Xenopus Development Time / Temperature Charts The rate of Xenopus development is influenced by temperature. Xenbase would like to thank Mustafa Khokha for making his data for these charts available to XENBASE ANATOMY ONTOLOGY: PAPERS FOR Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari. XENOPUS ATLAS: TISSUES: HEART Confocal images of Xenopus heart development submitted by Sandra Kolker and Dan Weeks.: Kolker SJ, Tajchman U, Weeks DL. Confocal imaging of early heart development in Xenopus laevis. Developmental Biology 218(1):64-73 OTX2 SUMMARY [SPECIES: XENOPUS LAEVIS AND XENOPUS Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari. Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox
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2020 XENOPUS WHITE PAPER The 2020 Xenopus Community White Paper is now available! PIS: PLEASE REFERENCE THE WHITE PAPER IN YOUR NIH GRANT PROPOSALS AND CONTACT YOUR PROGRAM OFFICERS. Download the 2020 Xenopus White Paper:Word Document
Read More...
*
XENOPUS, FROM BASIC BIOLOGY TO DISEASE MODELS IN THE GENOMIC ERA Abraham Fainsod and Sally Moody are seeking contributions for a book to be published by Taylor and Francis CRC Press. Please communicate your interest to them by May 15, 2020 and prepare to submit your contributions by January 2021.Read More...
*
THE ALLIED GENETICS CONFERENCE 2020 ONLINE TAGC is going virtual. April 22-25, 2020. REGISTRATION IS NOW OPEN.Attend via Zoom.
Click here
to view
the announcement.
TAGC2020 website
Read More...
*
18TH INTERNATIONAL XENOPUS CONFERENCE The meeting has been postponed to August 15-19, 2021. Portsmouth, UK at the EXRCRead More...
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UNCOVERING SELECTIVE COOPERATION BETWEEN THE RETINOBLASTOMA AND P53 CANCER PATHWAYS IN X. TROPICALIS Naert, Dimitrakopoulou et al. (2020) published in Oncogenetheir study
describing the first frog model for the Li-Fraumeni Syndrome to study leukemia and malignant sarcoma.Read More...
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SDB 79TH ANNUAL MEETING SOCIETY FOR DEVELOPMENTAL BIOLOGY 79TH ANNUAL MEETING Hyatt Regency Chicago, Chicago, ILJuly 9-13, 2020
Abstract submission deadline: April 1, 2020Read More...
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RAY KELLER AWARDED THE 2020 SDB LIFETIME ACHIEVEMENT AWARD Congratulations to Dr. Ray Keller on receiving the 2020 Society of Development Biology (SDB) Lifetime Achievement Award.
Ray is a pioneer in the biomechanics of morphogenesis introducing conversion extension in gastrulation to generations of Xenopusresearchers.
Read More...
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TRACHEA-ESOPHAGEAL DISEASE STUDIES USING XENOPUS Back to back papers published in Developmental Cell from Nasr et al.and
Kim et al.
recapitulate
human congenital trachea-esophageal birth defects using Xenopus.Read More...
*
XENOPUS TROPICALIS V10 GENOME - available to BLAST- view on JBrowse
99.86% of the genome mapped to chromosomes ~4500 new and refined models, 18% not present in trop v9.1Click here
to view the release document.Read More...
*
2020 XENOPUS WHITE PAPER The 2020 Xenopus Community White Paper is now available! PIS: PLEASE REFERENCE THE WHITE PAPER IN YOUR NIH GRANT PROPOSALS AND CONTACT YOUR PROGRAM OFFICERS. Download the 2020 Xenopus White Paper:Word Document
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BLAST XENOPUS
BLAST DATASETS:
Sequences
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X. laevis genomes
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* Nigerian 9.1
BLAST OTHER AMPHIBIANSBLAST ARCHIVES
BLAST ARCHIVED GENOMESX. LAEVIS GENOMES
* J-Strain 9.1
* J-Strain 8.0
* J-Strain 7.1
* J-Strain 6.0
X. TROPICALIS GENOMES* Nigerian 9.0
* Nigerian 8.0
* Nigerian 7.1
* Nigerian 4.1
GENOME BROWSERS
XENBASE BROWSERS:
X. laevis genomes
* J-Strain 9.2 on JBrowse * Wildtype 1.0 on JBrowse X. tropicalis genomes * Nigerian 10.0 on JBrowse * Nigerian 9.1 on JBrowseUCSC TRACK HUBS:
* Xenbase Track Hub
* Veenstra Lab Track HubUCSC GENOME BROWSER
BROWSE OTHER AMPHIBIANS ARCHIVES (INCL GBROWSE) BROWSER ARCHIVED GENOMESX. LAEVIS GENOMES
* J-Strain 9.1 on JBrowse * J-Strain 9.1 on GBrowse * J-Strain 8.0 on GBrowse * J-Strain 7.1 on JBrowse * J-Strain 7.1 on GBrowse * J-Strain 6.0 on GBrowse X. TROPICALIS GENOMES * Nigerian 9.0 on JBrowse * Nigerian 9.0 on GBrowse * Nigerian 8.0 on JBrowse * Nigerian 8.0 on GBrowse * Nigerian 7.1 on JBrowse * Nigerian 7.1 on GBrowse * Nigerian 4.1 on GBrowse OTHER GENOME BROWSERS* JGI
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GENE EXPRESSION
GENE EXPRESSION PATTERNS: * Gene Expression Search* Anatomy Search
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PROTEOMICS:
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ANNOUNCEMENTS
Xenopus, from basic biology to disease models in the genomic era book chapter submissions request The Allied Genetics Conference 2020 OnlineOnline conference
April 22-25, 2020
18th International Xenopus ConferencePortsmouth, UK
postponed to August 15-19, 2021 2020 Xenopus White PaperDownload: Word Doc
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IXB Young Investigator Award Nominations must be received by JUNE 30TH, 2020. Identification of Genetic and Genomic Variants by Next-Gen Sequencing in Non-human Animal Models (U01)PAR-15-120
Archived AnnouncementsWHY XENOPUS?
Xenopus is a tool for studying cell and developmental biology, and modeling human diseaseAbout Xenbase
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STOCK CENTERS
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XENOPUS RESOURCES
Gene Expression Datasets EctoMap: Ectodermal Diff. Expression Tail Regeneration @ U. Cambridge Embryogenesis @ Harvard U. Xenopus Data SourcesJGI (sequence)
XenMARK
XDB3
Xenopus tropicalis Resourcestrops @ U. Virginia
The Transcriptome of Xenopus tropicalis@ NIH
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* ABOUT _XENOPUS_
Xenbase: The _Xenopus_ laevis and _X._ tropicalis resource.Version: 4.13.1
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