2
More Annotations
5
6
Favourite Annotations
6
1
Text
REAGENTS FOR THE LIFE SCIENCES INDUSTRY 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com NEBNEXT® ARTIC SARS-COV-2 COMPANION KIT (OXFORD NANOPORE The NEBNext ® ARTIC kits were developed in response to the critical need for reliable and accurate methods for SARS-CoV-2 sequencing. These kits, for long and short read sequencing, were based on the original work of the ARTIC Network (1).The ARTIC SARS-CoV-2 sequencing workflow is a multiplexed amplicon-based whole-viral-genome sequencing
approach.
QUICK CIP | NEB
Quick CIP is a heat-labile recombinant version of calf intestinal alkaline phosphatase (CIP) purified from a recombinant source. Quick CIP nonspecifically catalyzes the dephosphorylation of 5′ and 3′ ends of DNA and RNA phosphomonoesters. It also hydrolyses ribo- LUNA® UNIVERSAL QPCR MASTER MIX Protocol for Two-step RT-qPCR using the LunaScript® RT SuperMix Kit (NEB #E3010) and the Luna® Universal qPCR Master Mix (NEB #M3003) or Luna Universal Probe qPCR Master Mix (NEB #M3004) Manuals. The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. manualM3003.AVRII | NEB
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.comNEBCUTTER V2.0
NEBcutter V2.0. This tool will take a DNA sequence and find the large, non-overlapping open reading frames using the E.coli genetic code and the sites for all Type II and commercially available Type III restriction enzymes that cut the sequence just once. By default, only enzymes available from NEB are used, but other sets may be chosen.BSU36I | NEB
Bsu36I. We are excited to announce that we are in the process of switching all reaction buffers to be BSA-free. Beginning April 2021, we will be gradually transitioning to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifyingenzymes.
Q5® HIGH-FIDELITY DNA POLYMERASE 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com NEBNEXT® QUICK LIGATION MODULE The NEBNext ® Quick Ligation Module has been optimized to ligate efficiently DNA adaptors compatible with Illumina ® sequencing to end-repaired, dA-tailed DNA fragments. The module is optimized for use with the NEBNext End Repair Module and the NEBNext dA-Tailing Module and is part of the original standard DNA library prep workflow, which is suitable for 1 - 5 μg of input DNA. NEB TM CALCULATORSEE MORE ON TMCALCULATOR.NEB.COM REAGENTS FOR THE LIFE SCIENCES INDUSTRY 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com NEBNEXT® ARTIC SARS-COV-2 COMPANION KIT (OXFORD NANOPORE The NEBNext ® ARTIC kits were developed in response to the critical need for reliable and accurate methods for SARS-CoV-2 sequencing. These kits, for long and short read sequencing, were based on the original work of the ARTIC Network (1).The ARTIC SARS-CoV-2 sequencing workflow is a multiplexed amplicon-based whole-viral-genome sequencingapproach.
QUICK CIP | NEB
Quick CIP is a heat-labile recombinant version of calf intestinal alkaline phosphatase (CIP) purified from a recombinant source. Quick CIP nonspecifically catalyzes the dephosphorylation of 5′ and 3′ ends of DNA and RNA phosphomonoesters. It also hydrolyses ribo- LUNA® UNIVERSAL QPCR MASTER MIX Protocol for Two-step RT-qPCR using the LunaScript® RT SuperMix Kit (NEB #E3010) and the Luna® Universal qPCR Master Mix (NEB #M3003) or Luna Universal Probe qPCR Master Mix (NEB #M3004) Manuals. The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. manualM3003.AVRII | NEB
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.comNEBCUTTER V2.0
NEBcutter V2.0. This tool will take a DNA sequence and find the large, non-overlapping open reading frames using the E.coli genetic code and the sites for all Type II and commercially available Type III restriction enzymes that cut the sequence just once. By default, only enzymes available from NEB are used, but other sets may be chosen.BSU36I | NEB
Bsu36I. We are excited to announce that we are in the process of switching all reaction buffers to be BSA-free. Beginning April 2021, we will be gradually transitioning to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifyingenzymes.
Q5® HIGH-FIDELITY DNA POLYMERASE 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com NEBNEXT® QUICK LIGATION MODULE The NEBNext ® Quick Ligation Module has been optimized to ligate efficiently DNA adaptors compatible with Illumina ® sequencing to end-repaired, dA-tailed DNA fragments. The module is optimized for use with the NEBNext End Repair Module and the NEBNext dA-Tailing Module and is part of the original standard DNA library prep workflow, which is suitable for 1 - 5 μg of input DNA. NEB TM CALCULATORSEE MORE ON TMCALCULATOR.NEB.COMPUBLICATIONS
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com LUNA® UNIVERSAL QPCR MASTER MIX Protocol for Two-step RT-qPCR using the LunaScript® RT SuperMix Kit (NEB #E3010) and the Luna® Universal qPCR Master Mix (NEB #M3003) or Luna Universal Probe qPCR Master Mix (NEB #M3004) Manuals. The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. manualM3003.AVRII | NEB
AvrII. We are excited to announce that we are in the process of switching all reaction buffers to be BSA-free. Beginning April 2021, we will be gradually transitioning to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifyingenzymes.
NEBNEXT® HIGH-FIDELITY 2X PCR MASTER MIX NEBNext ® High-Fidelity 2X PCR Master Mix is the original non-hot start NEBNext formulation of Q5 ® High-Fidelity DNA Polymerase. This formulation is also included as a component in the original NEBNext standard workflow library prep kits for Illumina, and in NEBNext library prep kits for Ion Torrent. Q5® HIGH-FIDELITY DNA POLYMERASE 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.comPUBLICATIONS
Search publications from New England Biolabs scientists. Are you doing COVID-19 related research? Our latest RUO kit, the Luna ® SARS-CoV-2 RT-qPCR Multiplex Assay Kit, enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes.For simple, visual assay results, the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit includes a color-changing pH LUNA® UNIVERSAL PROBE ONE-STEP RT-QPCR KIT The NEB Luna Universal Probe One-Step RT-qPCR Kit is optimized for real-time quantitation of target RNA sequences using hydrolysis probes. One-Step RT-qPCR provides a convenient and powerful method for RNA detection and quantitation. In a single tube, RNA is first converted to cDNA by a reverse transcriptase, and then a DNA-dependentDNA
LUNASCRIPT® RT MASTER MIX KIT (PRIMER-FREE) 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com SUPERSIZING MOLECULAR CLONING BY REWRITING THE RULES FOR Are you doing COVID-19 related research? Our latest RUO kit, the Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit, enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes.For simple, visual assay results, the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit includes a color-changing pH indicator for detection of SARS-CoV-2 nucleic acid NEBNEXT® QUICK LIGATION MODULE The NEBNext ® Quick Ligation Module has been optimized to ligate efficiently DNA adaptors compatible with Illumina ® sequencing to end-repaired, dA-tailed DNA fragments. The module is optimized for use with the NEBNext End Repair Module and the NEBNext dA-Tailing Module and is part of the original standard DNA library prep workflow, which is suitable for 1 - 5 μg of input DNA. REAGENTS FOR THE LIFE SCIENCES INDUSTRY 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com NEBNEXT® HIGH-FIDELITY 2X PCR MASTER MIX NEBNext ® High-Fidelity 2X PCR Master Mix is the original non-hot start NEBNext formulation of Q5 ® High-Fidelity DNA Polymerase. This formulation is also included as a component in the original NEBNext standard workflow library prep kits for Illumina, and in NEBNext library prep kits for Ion Torrent.QUICK CIP | NEB
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.comNEBCUTTER V2.0
NEBcutter - On-line DNA restriction mapper tool. This tool will take a DNA sequence and find the large, non-overlapping open reading frames using the E.coli genetic code and the sites for all Type II and commercially available Type III restriction enzymes that cut thesequence just once.
BSU36I | NEB
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.comBSTXI | NEB
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com DEEP VENT® DNA POLYMERASE 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com NEBNEXT® QUICK LIGATION MODULE The NEBNext ® Quick Ligation Module has been optimized to ligate efficiently DNA adaptors compatible with Illumina ® sequencing to end-repaired, dA-tailed DNA fragments. The module is optimized for use with the NEBNext End Repair Module and the NEBNext dA-Tailing Module and is part of the original standard DNA library prep workflow, which is suitable for 1 - 5 μg of input DNA. NEB TM CALCULATORSEE MORE ON TMCALCULATOR.NEB.COMNEBASECHANGER
NEBaseChanger can design primers specific to the mutagenesis experiment you are performing and calculate a recommended custom annealing temperature. REAGENTS FOR THE LIFE SCIENCES INDUSTRY 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com NEBNEXT® HIGH-FIDELITY 2X PCR MASTER MIX NEBNext ® High-Fidelity 2X PCR Master Mix is the original non-hot start NEBNext formulation of Q5 ® High-Fidelity DNA Polymerase. This formulation is also included as a component in the original NEBNext standard workflow library prep kits for Illumina, and in NEBNext library prep kits for Ion Torrent.QUICK CIP | NEB
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.comNEBCUTTER V2.0
NEBcutter - On-line DNA restriction mapper tool. This tool will take a DNA sequence and find the large, non-overlapping open reading frames using the E.coli genetic code and the sites for all Type II and commercially available Type III restriction enzymes that cut thesequence just once.
BSU36I | NEB
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.comBSTXI | NEB
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com DEEP VENT® DNA POLYMERASE 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com NEBNEXT® QUICK LIGATION MODULE The NEBNext ® Quick Ligation Module has been optimized to ligate efficiently DNA adaptors compatible with Illumina ® sequencing to end-repaired, dA-tailed DNA fragments. The module is optimized for use with the NEBNext End Repair Module and the NEBNext dA-Tailing Module and is part of the original standard DNA library prep workflow, which is suitable for 1 - 5 μg of input DNA. NEB TM CALCULATORSEE MORE ON TMCALCULATOR.NEB.COMNEBASECHANGER
NEBaseChanger can design primers specific to the mutagenesis experiment you are performing and calculate a recommended custom annealing temperature.PUBLICATIONS
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.comAVRII | NEB
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.comBSTXI | NEB
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com Q5® HIGH-FIDELITY DNA POLYMERASE 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com NEW ENGLAND BIOLABS®, INC. LAUNCHES PRIMER MONITOR TOOL Are you doing COVID-19 related research? Our latest RUO kit, the Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit, enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes.For simple, visual assay results, the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit includes a color-changing pH indicator for detection of SARS-CoV-2 nucleic acid amplificationPUBLICATIONS
Are you doing COVID-19 related research? Our latest RUO kit, the Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit, enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes.For simple, visual assay results, the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit includes a color-changing pH indicator for detection of SARS-CoV-2 nucleic acid amplification NEBNEXT® QUICK LIGATION MODULE The NEBNext ® Quick Ligation Module has been optimized to ligate efficiently DNA adaptors compatible with Illumina ® sequencing to end-repaired, dA-tailed DNA fragments. The module is optimized for use with the NEBNext End Repair Module and the NEBNext dA-Tailing Module and is part of the original standard DNA library prep workflow, which is suitable for 1 - 5 μg of input DNA.PUBLICATIONS
Search publications from New England Biolabs scientists. Are you doing COVID-19 related research? Our latest RUO kit, the Luna ® SARS-CoV-2 RT-qPCR Multiplex Assay Kit, enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes.For simple, visual assay results, the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit includes a color-changing pHVIDEO LIBRARY
Increase Productivity & Reproducibility by Automating DNA Library Prep for Ion Torrent ® Sequencing. Learn how Biomek automation of NEBNext DNA library preparation for Ion Torrent enables high-throughput, reproducible production of libraries with high yield and complexity.NEBIOCALCULATOR
Choose a DNA, RNA, genome editing, qPCR calculator from NEB, a leader in production and supply of reagents for the life science industry. REAGENTS FOR THE LIFE SCIENCES INDUSTRY 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com NEBNEXT® HIGH-FIDELITY 2X PCR MASTER MIX NEBNext ® High-Fidelity 2X PCR Master Mix is the original non-hot start NEBNext formulation of Q5 ® High-Fidelity DNA Polymerase. This formulation is also included as a component in the original NEBNext standard workflow library prep kits for Illumina, and in NEBNext library prep kits for Ion Torrent. LUNA® UNIVERSAL QPCR MASTER MIX Protocol for Two-step RT-qPCR using the LunaScript® RT SuperMix Kit (NEB #E3010) and the Luna® Universal qPCR Master Mix (NEB #M3003) or Luna Universal Probe qPCR Master Mix (NEB #M3004) Manuals. The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. manualM3003.NEBCUTTER V2.0
NEBcutter V2.0. This tool will take a DNA sequence and find the large, non-overlapping open reading frames using the E.coli genetic code and the sites for all Type II and commercially available Type III restriction enzymes that cut the sequence just once. By default, only enzymes available from NEB are used, but other sets may be chosen.BSTXI | NEB
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.comQUICK CIP | NEB
Quick CIP is a heat-labile recombinant version of calf intestinal alkaline phosphatase (CIP) purified from a recombinant source. Quick CIP nonspecifically catalyzes the dephosphorylation of 5′ and 3′ ends of DNA and RNA phosphomonoesters. It also hydrolyses ribo- NEB® TURBO COMPETENT E. COLI (HIGH EFFICIENCY) 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com Q5® HIGH-FIDELITY DNA POLYMERASE 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com PHUSION® HIGH-FIDELITY DNA POLYMERASE Phusion DNA Polymerase is supplied with standard 5X Phusion HF Buffer, as well as 5X Phusion GC Buffer, which can be used for complex or GC-rich templates. Each of these buffers contains MgCl 2 (1.5 mM at the final reaction concentration). Reactions can also be optimized using the provided DMSO or MgCl 2 solutions. MONARCH® PCR & DNA CLEANUP KIT (5 ΜG) Monarch PCR & DNA Cleanup Kit (5 μg) removes low molecular weight primers from dsDNA samples. Three independent amplicons (267 bp, 520 bp, 1003 bp) were spiked with two oligonucleotides (16-mer, 24-mer) to a final concentration of 1 μM. Half of each mix was purified with the Monarch PCR & DNA Cleanup Kit (5 μg) following the included protocol. REAGENTS FOR THE LIFE SCIENCES INDUSTRY 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com NEBNEXT® HIGH-FIDELITY 2X PCR MASTER MIX NEBNext ® High-Fidelity 2X PCR Master Mix is the original non-hot start NEBNext formulation of Q5 ® High-Fidelity DNA Polymerase. This formulation is also included as a component in the original NEBNext standard workflow library prep kits for Illumina, and in NEBNext library prep kits for Ion Torrent. LUNA® UNIVERSAL QPCR MASTER MIX Protocol for Two-step RT-qPCR using the LunaScript® RT SuperMix Kit (NEB #E3010) and the Luna® Universal qPCR Master Mix (NEB #M3003) or Luna Universal Probe qPCR Master Mix (NEB #M3004) Manuals. The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. manualM3003.NEBCUTTER V2.0
NEBcutter V2.0. This tool will take a DNA sequence and find the large, non-overlapping open reading frames using the E.coli genetic code and the sites for all Type II and commercially available Type III restriction enzymes that cut the sequence just once. By default, only enzymes available from NEB are used, but other sets may be chosen.BSTXI | NEB
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.comQUICK CIP | NEB
Quick CIP is a heat-labile recombinant version of calf intestinal alkaline phosphatase (CIP) purified from a recombinant source. Quick CIP nonspecifically catalyzes the dephosphorylation of 5′ and 3′ ends of DNA and RNA phosphomonoesters. It also hydrolyses ribo- NEB® TURBO COMPETENT E. COLI (HIGH EFFICIENCY) 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com Q5® HIGH-FIDELITY DNA POLYMERASE 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com PHUSION® HIGH-FIDELITY DNA POLYMERASE Phusion DNA Polymerase is supplied with standard 5X Phusion HF Buffer, as well as 5X Phusion GC Buffer, which can be used for complex or GC-rich templates. Each of these buffers contains MgCl 2 (1.5 mM at the final reaction concentration). Reactions can also be optimized using the provided DMSO or MgCl 2 solutions. MONARCH® PCR & DNA CLEANUP KIT (5 ΜG) Monarch PCR & DNA Cleanup Kit (5 μg) removes low molecular weight primers from dsDNA samples. Three independent amplicons (267 bp, 520 bp, 1003 bp) were spiked with two oligonucleotides (16-mer, 24-mer) to a final concentration of 1 μM. Half of each mix was purified with the Monarch PCR & DNA Cleanup Kit (5 μg) following the included protocol.PUBLICATIONS
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com LUNA® UNIVERSAL QPCR MASTER MIX Protocol for Two-step RT-qPCR using the LunaScript® RT SuperMix Kit (NEB #E3010) and the Luna® Universal qPCR Master Mix (NEB #M3003) or Luna Universal Probe qPCR Master Mix (NEB #M3004) Manuals. The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. manualM3003. ALPHABETIZED LIST OF RECOGNITION SPECIFICITIES Alphabetized List of Recognition Specificities. All restriction endonuclease recognition specificities available from New England Biolabs are listed below. For enzymes that recognize non-palindromic sequences, the complementary sequence of each strand is listed. For example, CCTC (7/6) and (6/7)GAGG both represent an MnlI ( NEB #R0163)site.
BSAI | NEB
Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled quality. 100% activity in CutSmart Buffer (over 215 enzymes are available in the same buffer) simplifying double digests. BsaI has a High Fidelityversion BsaI
NEBNEXT ULTRA II DIRECTIONAL RNA LIBRARY PREP KIT FOR The NEBNext Ultra II Directional RNA Library Prep Kit derives its directionality from the “dUTP” method for strand-specificity, with proven superiority for this application. See what customers are saying about NEBNext Ultra II Directional RNA. Features. Get more of what you need, with the highest library yields.DOUBLE DIGESTS
Double digests with NEB's restriction enzymes can be set up in rCutSmart Buffer™. Otherwise, choose an NEBuffer that results in the most activity for both enzymes. If star activity is a concern, consider using one of our High Fidelity (HF®) enzymes. Set up reaction according to recommended protocol. The final concentration ofglycerol in any
PUBLICATIONS
Are you doing COVID-19 related research? Our latest RUO kit, the Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit, enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes.For simple, visual assay results, the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit includes a color-changing pH indicator for detection of SARS-CoV-2 nucleic acid amplificationPUBLICATIONS
Search publications from New England Biolabs scientists. Are you doing COVID-19 related research? Our latest RUO kit, the Luna ® SARS-CoV-2 RT-qPCR Multiplex Assay Kit, enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes.For simple, visual assay results, the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit includes a color-changing pHVIDEO LIBRARY
Increase Productivity & Reproducibility by Automating DNA Library Prep for Ion Torrent ® Sequencing. Learn how Biomek automation of NEBNext DNA library preparation for Ion Torrent enables high-throughput, reproducible production of libraries with high yield and complexity.ENZYME FINDER
Locate commercially available restriction enzymes by category, name, recognition sequence, or overhang. REAGENTS FOR THE LIFE SCIENCES INDUSTRY 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com NEBNEXT® HIGH-FIDELITY 2X PCR MASTER MIX NEBNext ® High-Fidelity 2X PCR Master Mix is the original non-hot start NEBNext formulation of Q5 ® High-Fidelity DNA Polymerase. This formulation is also included as a component in the original NEBNext standard workflow library prep kits for Illumina, and in NEBNext library prep kits for Ion Torrent.QUICK CIP | NEB
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.comNEBCUTTER V2.0
NEBcutter - On-line DNA restriction mapper tool. This tool will take a DNA sequence and find the large, non-overlapping open reading frames using the E.coli genetic code and the sites for all Type II and commercially available Type III restriction enzymes that cut thesequence just once.
BSU36I | NEB
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.comBSTXI | NEB
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com DEEP VENT® DNA POLYMERASE 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com NEBNEXT® QUICK LIGATION MODULE The NEBNext ® Quick Ligation Module has been optimized to ligate efficiently DNA adaptors compatible with Illumina ® sequencing to end-repaired, dA-tailed DNA fragments. The module is optimized for use with the NEBNext End Repair Module and the NEBNext dA-Tailing Module and is part of the original standard DNA library prep workflow, which is suitable for 1 - 5 μg of input DNA. NEB TM CALCULATORSEE MORE ON TMCALCULATOR.NEB.COMNEBASECHANGER
NEBaseChanger can design primers specific to the mutagenesis experiment you are performing and calculate a recommended custom annealing temperature. REAGENTS FOR THE LIFE SCIENCES INDUSTRY 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com NEBNEXT® HIGH-FIDELITY 2X PCR MASTER MIX NEBNext ® High-Fidelity 2X PCR Master Mix is the original non-hot start NEBNext formulation of Q5 ® High-Fidelity DNA Polymerase. This formulation is also included as a component in the original NEBNext standard workflow library prep kits for Illumina, and in NEBNext library prep kits for Ion Torrent.QUICK CIP | NEB
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.comNEBCUTTER V2.0
NEBcutter - On-line DNA restriction mapper tool. This tool will take a DNA sequence and find the large, non-overlapping open reading frames using the E.coli genetic code and the sites for all Type II and commercially available Type III restriction enzymes that cut thesequence just once.
BSU36I | NEB
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.comBSTXI | NEB
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com DEEP VENT® DNA POLYMERASE 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com NEBNEXT® QUICK LIGATION MODULE The NEBNext ® Quick Ligation Module has been optimized to ligate efficiently DNA adaptors compatible with Illumina ® sequencing to end-repaired, dA-tailed DNA fragments. The module is optimized for use with the NEBNext End Repair Module and the NEBNext dA-Tailing Module and is part of the original standard DNA library prep workflow, which is suitable for 1 - 5 μg of input DNA. NEB TM CALCULATORSEE MORE ON TMCALCULATOR.NEB.COMNEBASECHANGER
NEBaseChanger can design primers specific to the mutagenesis experiment you are performing and calculate a recommended custom annealing temperature.PUBLICATIONS
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.comAVRII | NEB
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.comBSTXI | NEB
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com Q5® HIGH-FIDELITY DNA POLYMERASE 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com NEW ENGLAND BIOLABS®, INC. LAUNCHES PRIMER MONITOR TOOL Are you doing COVID-19 related research? Our latest RUO kit, the Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit, enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes.For simple, visual assay results, the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit includes a color-changing pH indicator for detection of SARS-CoV-2 nucleic acid amplificationPUBLICATIONS
Are you doing COVID-19 related research? Our latest RUO kit, the Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit, enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes.For simple, visual assay results, the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit includes a color-changing pH indicator for detection of SARS-CoV-2 nucleic acid amplification NEBNEXT® QUICK LIGATION MODULE The NEBNext ® Quick Ligation Module has been optimized to ligate efficiently DNA adaptors compatible with Illumina ® sequencing to end-repaired, dA-tailed DNA fragments. The module is optimized for use with the NEBNext End Repair Module and the NEBNext dA-Tailing Module and is part of the original standard DNA library prep workflow, which is suitable for 1 - 5 μg of input DNA.PUBLICATIONS
Search publications from New England Biolabs scientists. Are you doing COVID-19 related research? Our latest RUO kit, the Luna ® SARS-CoV-2 RT-qPCR Multiplex Assay Kit, enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes.For simple, visual assay results, the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit includes a color-changing pHVIDEO LIBRARY
Increase Productivity & Reproducibility by Automating DNA Library Prep for Ion Torrent ® Sequencing. Learn how Biomek automation of NEBNext DNA library preparation for Ion Torrent enables high-throughput, reproducible production of libraries with high yield and complexity.NEBIOCALCULATOR
Choose a DNA, RNA, genome editing, qPCR calculator from NEB, a leader in production and supply of reagents for the life science industry. REAGENTS FOR THE LIFE SCIENCES INDUSTRY 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com NEBNEXT® HIGH-FIDELITY 2X PCR MASTER MIX NEBNext ® High-Fidelity 2X PCR Master Mix is the original non-hot start NEBNext formulation of Q5 ® High-Fidelity DNA Polymerase. This formulation is also included as a component in the original NEBNext standard workflow library prep kits for Illumina, and in NEBNext library prep kits for Ion Torrent. LUNA® UNIVERSAL QPCR MASTER MIX Protocol for Two-step RT-qPCR using the LunaScript® RT SuperMix Kit (NEB #E3010) and the Luna® Universal qPCR Master Mix (NEB #M3003) or Luna Universal Probe qPCR Master Mix (NEB #M3004) Manuals. The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. manualM3003.NEBCUTTER V2.0
NEBcutter V2.0. This tool will take a DNA sequence and find the large, non-overlapping open reading frames using the E.coli genetic code and the sites for all Type II and commercially available Type III restriction enzymes that cut the sequence just once. By default, only enzymes available from NEB are used, but other sets may be chosen.BSTXI | NEB
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.comQUICK CIP | NEB
Quick CIP is a heat-labile recombinant version of calf intestinal alkaline phosphatase (CIP) purified from a recombinant source. Quick CIP nonspecifically catalyzes the dephosphorylation of 5′ and 3′ ends of DNA and RNA phosphomonoesters. It also hydrolyses ribo- NEB® TURBO COMPETENT E. COLI (HIGH EFFICIENCY) 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com Q5® HIGH-FIDELITY DNA POLYMERASE 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com PHUSION® HIGH-FIDELITY DNA POLYMERASE Phusion DNA Polymerase is supplied with standard 5X Phusion HF Buffer, as well as 5X Phusion GC Buffer, which can be used for complex or GC-rich templates. Each of these buffers contains MgCl 2 (1.5 mM at the final reaction concentration). Reactions can also be optimized using the provided DMSO or MgCl 2 solutions. MONARCH® PCR & DNA CLEANUP KIT (5 ΜG) Monarch PCR & DNA Cleanup Kit (5 μg) removes low molecular weight primers from dsDNA samples. Three independent amplicons (267 bp, 520 bp, 1003 bp) were spiked with two oligonucleotides (16-mer, 24-mer) to a final concentration of 1 μM. Half of each mix was purified with the Monarch PCR & DNA Cleanup Kit (5 μg) following the included protocol. REAGENTS FOR THE LIFE SCIENCES INDUSTRY 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com NEBNEXT® HIGH-FIDELITY 2X PCR MASTER MIX NEBNext ® High-Fidelity 2X PCR Master Mix is the original non-hot start NEBNext formulation of Q5 ® High-Fidelity DNA Polymerase. This formulation is also included as a component in the original NEBNext standard workflow library prep kits for Illumina, and in NEBNext library prep kits for Ion Torrent. LUNA® UNIVERSAL QPCR MASTER MIX Protocol for Two-step RT-qPCR using the LunaScript® RT SuperMix Kit (NEB #E3010) and the Luna® Universal qPCR Master Mix (NEB #M3003) or Luna Universal Probe qPCR Master Mix (NEB #M3004) Manuals. The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. manualM3003.NEBCUTTER V2.0
NEBcutter V2.0. This tool will take a DNA sequence and find the large, non-overlapping open reading frames using the E.coli genetic code and the sites for all Type II and commercially available Type III restriction enzymes that cut the sequence just once. By default, only enzymes available from NEB are used, but other sets may be chosen.BSTXI | NEB
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.comQUICK CIP | NEB
Quick CIP is a heat-labile recombinant version of calf intestinal alkaline phosphatase (CIP) purified from a recombinant source. Quick CIP nonspecifically catalyzes the dephosphorylation of 5′ and 3′ ends of DNA and RNA phosphomonoesters. It also hydrolyses ribo- NEB® TURBO COMPETENT E. COLI (HIGH EFFICIENCY) 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com Q5® HIGH-FIDELITY DNA POLYMERASE 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com PHUSION® HIGH-FIDELITY DNA POLYMERASE Phusion DNA Polymerase is supplied with standard 5X Phusion HF Buffer, as well as 5X Phusion GC Buffer, which can be used for complex or GC-rich templates. Each of these buffers contains MgCl 2 (1.5 mM at the final reaction concentration). Reactions can also be optimized using the provided DMSO or MgCl 2 solutions. MONARCH® PCR & DNA CLEANUP KIT (5 ΜG) Monarch PCR & DNA Cleanup Kit (5 μg) removes low molecular weight primers from dsDNA samples. Three independent amplicons (267 bp, 520 bp, 1003 bp) were spiked with two oligonucleotides (16-mer, 24-mer) to a final concentration of 1 μM. Half of each mix was purified with the Monarch PCR & DNA Cleanup Kit (5 μg) following the included protocol.PUBLICATIONS
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com LUNA® UNIVERSAL QPCR MASTER MIX Protocol for Two-step RT-qPCR using the LunaScript® RT SuperMix Kit (NEB #E3010) and the Luna® Universal qPCR Master Mix (NEB #M3003) or Luna Universal Probe qPCR Master Mix (NEB #M3004) Manuals. The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. manualM3003. ALPHABETIZED LIST OF RECOGNITION SPECIFICITIES Alphabetized List of Recognition Specificities. All restriction endonuclease recognition specificities available from New England Biolabs are listed below. For enzymes that recognize non-palindromic sequences, the complementary sequence of each strand is listed. For example, CCTC (7/6) and (6/7)GAGG both represent an MnlI ( NEB #R0163)site.
BSAI | NEB
Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled quality. 100% activity in CutSmart Buffer (over 215 enzymes are available in the same buffer) simplifying double digests. BsaI has a High Fidelityversion BsaI
NEBNEXT ULTRA II DIRECTIONAL RNA LIBRARY PREP KIT FOR The NEBNext Ultra II Directional RNA Library Prep Kit derives its directionality from the “dUTP” method for strand-specificity, with proven superiority for this application. See what customers are saying about NEBNext Ultra II Directional RNA. Features. Get more of what you need, with the highest library yields.DOUBLE DIGESTS
Double digests with NEB's restriction enzymes can be set up in rCutSmart Buffer™. Otherwise, choose an NEBuffer that results in the most activity for both enzymes. If star activity is a concern, consider using one of our High Fidelity (HF®) enzymes. Set up reaction according to recommended protocol. The final concentration ofglycerol in any
PUBLICATIONS
Are you doing COVID-19 related research? Our latest RUO kit, the Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit, enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes.For simple, visual assay results, the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit includes a color-changing pH indicator for detection of SARS-CoV-2 nucleic acid amplificationPUBLICATIONS
Search publications from New England Biolabs scientists. Are you doing COVID-19 related research? Our latest RUO kit, the Luna ® SARS-CoV-2 RT-qPCR Multiplex Assay Kit, enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes.For simple, visual assay results, the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit includes a color-changing pHVIDEO LIBRARY
Increase Productivity & Reproducibility by Automating DNA Library Prep for Ion Torrent ® Sequencing. Learn how Biomek automation of NEBNext DNA library preparation for Ion Torrent enables high-throughput, reproducible production of libraries with high yield and complexity.ENZYME FINDER
Locate commercially available restriction enzymes by category, name, recognition sequence, or overhang. REAGENTS FOR THE LIFE SCIENCES INDUSTRY 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com NEBNEXT® HIGH-FIDELITY 2X PCR MASTER MIX NEBNext ® High-Fidelity 2X PCR Master Mix is the original non-hot start NEBNext formulation of Q5 ® High-Fidelity DNA Polymerase. This formulation is also included as a component in the original NEBNext standard workflow library prep kits for Illumina, and in NEBNext library prep kits for Ion Torrent. LUNA® UNIVERSAL QPCR MASTER MIX Protocol for Two-step RT-qPCR using the LunaScript® RT SuperMix Kit (NEB #E3010) and the Luna® Universal qPCR Master Mix (NEB #M3003) or Luna Universal Probe qPCR Master Mix (NEB #M3004) Manuals. The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. manualM3003.NEBCUTTER V2.0
NEBcutter V2.0. This tool will take a DNA sequence and find the large, non-overlapping open reading frames using the E.coli genetic code and the sites for all Type II and commercially available Type III restriction enzymes that cut the sequence just once. By default, only enzymes available from NEB are used, but other sets may be chosen.BSTXI | NEB
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.comQUICK CIP | NEB
Quick CIP is a heat-labile recombinant version of calf intestinal alkaline phosphatase (CIP) purified from a recombinant source. Quick CIP nonspecifically catalyzes the dephosphorylation of 5′ and 3′ ends of DNA and RNA phosphomonoesters. It also hydrolyses ribo- NEB® TURBO COMPETENT E. COLI (HIGH EFFICIENCY) 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com Q5® HIGH-FIDELITY DNA POLYMERASE 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com PHUSION® HIGH-FIDELITY DNA POLYMERASE Phusion DNA Polymerase is supplied with standard 5X Phusion HF Buffer, as well as 5X Phusion GC Buffer, which can be used for complex or GC-rich templates. Each of these buffers contains MgCl 2 (1.5 mM at the final reaction concentration). Reactions can also be optimized using the provided DMSO or MgCl 2 solutions. MONARCH® PCR & DNA CLEANUP KIT (5 ΜG) Monarch PCR & DNA Cleanup Kit (5 μg) removes low molecular weight primers from dsDNA samples. Three independent amplicons (267 bp, 520 bp, 1003 bp) were spiked with two oligonucleotides (16-mer, 24-mer) to a final concentration of 1 μM. Half of each mix was purified with the Monarch PCR & DNA Cleanup Kit (5 μg) following the included protocol. REAGENTS FOR THE LIFE SCIENCES INDUSTRY 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com NEBNEXT® HIGH-FIDELITY 2X PCR MASTER MIX NEBNext ® High-Fidelity 2X PCR Master Mix is the original non-hot start NEBNext formulation of Q5 ® High-Fidelity DNA Polymerase. This formulation is also included as a component in the original NEBNext standard workflow library prep kits for Illumina, and in NEBNext library prep kits for Ion Torrent. LUNA® UNIVERSAL QPCR MASTER MIX Protocol for Two-step RT-qPCR using the LunaScript® RT SuperMix Kit (NEB #E3010) and the Luna® Universal qPCR Master Mix (NEB #M3003) or Luna Universal Probe qPCR Master Mix (NEB #M3004) Manuals. The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. manualM3003.NEBCUTTER V2.0
NEBcutter V2.0. This tool will take a DNA sequence and find the large, non-overlapping open reading frames using the E.coli genetic code and the sites for all Type II and commercially available Type III restriction enzymes that cut the sequence just once. By default, only enzymes available from NEB are used, but other sets may be chosen.BSTXI | NEB
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.comQUICK CIP | NEB
Quick CIP is a heat-labile recombinant version of calf intestinal alkaline phosphatase (CIP) purified from a recombinant source. Quick CIP nonspecifically catalyzes the dephosphorylation of 5′ and 3′ ends of DNA and RNA phosphomonoesters. It also hydrolyses ribo- NEB® TURBO COMPETENT E. COLI (HIGH EFFICIENCY) 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com Q5® HIGH-FIDELITY DNA POLYMERASE 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com PHUSION® HIGH-FIDELITY DNA POLYMERASE Phusion DNA Polymerase is supplied with standard 5X Phusion HF Buffer, as well as 5X Phusion GC Buffer, which can be used for complex or GC-rich templates. Each of these buffers contains MgCl 2 (1.5 mM at the final reaction concentration). Reactions can also be optimized using the provided DMSO or MgCl 2 solutions. MONARCH® PCR & DNA CLEANUP KIT (5 ΜG) Monarch PCR & DNA Cleanup Kit (5 μg) removes low molecular weight primers from dsDNA samples. Three independent amplicons (267 bp, 520 bp, 1003 bp) were spiked with two oligonucleotides (16-mer, 24-mer) to a final concentration of 1 μM. Half of each mix was purified with the Monarch PCR & DNA Cleanup Kit (5 μg) following the included protocol.PUBLICATIONS
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com LUNA® UNIVERSAL QPCR MASTER MIX Protocol for Two-step RT-qPCR using the LunaScript® RT SuperMix Kit (NEB #E3010) and the Luna® Universal qPCR Master Mix (NEB #M3003) or Luna Universal Probe qPCR Master Mix (NEB #M3004) Manuals. The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. manualM3003. ALPHABETIZED LIST OF RECOGNITION SPECIFICITIES Alphabetized List of Recognition Specificities. All restriction endonuclease recognition specificities available from New England Biolabs are listed below. For enzymes that recognize non-palindromic sequences, the complementary sequence of each strand is listed. For example, CCTC (7/6) and (6/7)GAGG both represent an MnlI ( NEB #R0163)site.
BSAI | NEB
Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled quality. 100% activity in CutSmart Buffer (over 215 enzymes are available in the same buffer) simplifying double digests. BsaI has a High Fidelityversion BsaI
NEBNEXT ULTRA II DIRECTIONAL RNA LIBRARY PREP KIT FOR The NEBNext Ultra II Directional RNA Library Prep Kit derives its directionality from the “dUTP” method for strand-specificity, with proven superiority for this application. See what customers are saying about NEBNext Ultra II Directional RNA. Features. Get more of what you need, with the highest library yields.DOUBLE DIGESTS
Double digests with NEB's restriction enzymes can be set up in rCutSmart Buffer™. Otherwise, choose an NEBuffer that results in the most activity for both enzymes. If star activity is a concern, consider using one of our High Fidelity (HF®) enzymes. Set up reaction according to recommended protocol. The final concentration ofglycerol in any
PUBLICATIONS
Are you doing COVID-19 related research? Our latest RUO kit, the Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit, enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes.For simple, visual assay results, the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit includes a color-changing pH indicator for detection of SARS-CoV-2 nucleic acid amplificationPUBLICATIONS
Search publications from New England Biolabs scientists. Are you doing COVID-19 related research? Our latest RUO kit, the Luna ® SARS-CoV-2 RT-qPCR Multiplex Assay Kit, enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes.For simple, visual assay results, the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit includes a color-changing pHVIDEO LIBRARY
Increase Productivity & Reproducibility by Automating DNA Library Prep for Ion Torrent ® Sequencing. Learn how Biomek automation of NEBNext DNA library preparation for Ion Torrent enables high-throughput, reproducible production of libraries with high yield and complexity.ENZYME FINDER
Locate commercially available restriction enzymes by category, name, recognition sequence, or overhang. REAGENTS FOR THE LIFE SCIENCES INDUSTRY 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.comAGEI | NEB
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com NEBNEXT® HIGH-FIDELITY 2X PCR MASTER MIX NEBNext ® High-Fidelity 2X PCR Master Mix is the original non-hot start NEBNext formulation of Q5 ® High-Fidelity DNA Polymerase. This formulation is also included as a component in the original NEBNext standard workflow library prep kits for Illumina, and in NEBNext library prep kits for Ion Torrent.NEBCUTTER V2.0
NEBcutter V2.0. This tool will take a DNA sequence and find the large, non-overlapping open reading frames using the E.coli genetic code and the sites for all Type II and commercially available Type III restriction enzymes that cut the sequence just once. By default, only enzymes available from NEB are used, but other sets may be chosen.QUICK CIP | NEB
Quick CIP is a heat-labile recombinant version of calf intestinal alkaline phosphatase (CIP) purified from a recombinant source. Quick CIP nonspecifically catalyzes the dephosphorylation of 5′ and 3′ ends of DNA and RNA phosphomonoesters. It also hydrolyses ribo-BSU36I | NEB
Bsu36I. We are excited to announce that we are in the process of switching all reaction buffers to be BSA-free. Beginning April 2021, we will be gradually transitioning to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifyingenzymes.
BSTXI | NEB
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com DEEP VENT® DNA POLYMERASE Deep Vent DNA Polymerase was the second high-fidelity thermophilic DNA polymerase available from New England Biolabs. The fidelity of Deep Vent DNA Polymerase is derived in part from an integral 3´→ 5´ proofreading exonuclease activity. Deep Vent is even more stable thanVent at
NEBNEXT® QUICK LIGATION MODULE The NEBNext ® Quick Ligation Module has been optimized to ligate efficiently DNA adaptors compatible with Illumina ® sequencing to end-repaired, dA-tailed DNA fragments. The module is optimized for use with the NEBNext End Repair Module and the NEBNext dA-Tailing Module and is part of the original standard DNA library prep workflow, which is suitable for 1 - 5 μg of input DNA. NEB TM CALCULATORSEE MORE ON TMCALCULATOR.NEB.COM REAGENTS FOR THE LIFE SCIENCES INDUSTRY 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.comAGEI | NEB
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com NEBNEXT® HIGH-FIDELITY 2X PCR MASTER MIX NEBNext ® High-Fidelity 2X PCR Master Mix is the original non-hot start NEBNext formulation of Q5 ® High-Fidelity DNA Polymerase. This formulation is also included as a component in the original NEBNext standard workflow library prep kits for Illumina, and in NEBNext library prep kits for Ion Torrent.NEBCUTTER V2.0
NEBcutter V2.0. This tool will take a DNA sequence and find the large, non-overlapping open reading frames using the E.coli genetic code and the sites for all Type II and commercially available Type III restriction enzymes that cut the sequence just once. By default, only enzymes available from NEB are used, but other sets may be chosen.QUICK CIP | NEB
Quick CIP is a heat-labile recombinant version of calf intestinal alkaline phosphatase (CIP) purified from a recombinant source. Quick CIP nonspecifically catalyzes the dephosphorylation of 5′ and 3′ ends of DNA and RNA phosphomonoesters. It also hydrolyses ribo-BSU36I | NEB
Bsu36I. We are excited to announce that we are in the process of switching all reaction buffers to be BSA-free. Beginning April 2021, we will be gradually transitioning to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifyingenzymes.
BSTXI | NEB
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com DEEP VENT® DNA POLYMERASE Deep Vent DNA Polymerase was the second high-fidelity thermophilic DNA polymerase available from New England Biolabs. The fidelity of Deep Vent DNA Polymerase is derived in part from an integral 3´→ 5´ proofreading exonuclease activity. Deep Vent is even more stable thanVent at
NEBNEXT® QUICK LIGATION MODULE The NEBNext ® Quick Ligation Module has been optimized to ligate efficiently DNA adaptors compatible with Illumina ® sequencing to end-repaired, dA-tailed DNA fragments. The module is optimized for use with the NEBNext End Repair Module and the NEBNext dA-Tailing Module and is part of the original standard DNA library prep workflow, which is suitable for 1 - 5 μg of input DNA. NEB TM CALCULATORSEE MORE ON TMCALCULATOR.NEB.COMAGEI | NEB
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.comPUBLICATIONS
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.comBSTXI | NEB
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com Q5® HIGH-FIDELITY DNA POLYMERASE 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.comAATII | NEB
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com DEEP VENT® DNA POLYMERASE Deep Vent DNA Polymerase was the second high-fidelity thermophilic DNA polymerase available from New England Biolabs. The fidelity of Deep Vent DNA Polymerase is derived in part from an integral 3´→ 5´ proofreading exonuclease activity. Deep Vent is even more stable thanVent at
VIDEO LIBRARY
Increase Productivity & Reproducibility by Automating DNA Library Prep for Ion Torrent ® Sequencing. Learn how Biomek automation of NEBNext DNA library preparation for Ion Torrent enables high-throughput, reproducible production of libraries with high yield and complexity.PUBLICATIONS
Are you doing COVID-19 related research? Our latest RUO kit, the Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit, enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes.For simple, visual assay results, the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit includes a color-changing pH indicator for detection of SARS-CoV-2 nucleic acid amplificationPUBLICATIONS
Search publications from New England Biolabs scientists. Are you doing COVID-19 related research? Our latest RUO kit, the Luna ® SARS-CoV-2 RT-qPCR Multiplex Assay Kit, enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes.For simple, visual assay results, the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit includes a color-changing pHENZYME FINDER
Locate commercially available restriction enzymes by category, name, recognition sequence, or overhang. REAGENTS FOR THE LIFE SCIENCES INDUSTRY 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.comAGEI | NEB
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com NEBNEXT® HIGH-FIDELITY 2X PCR MASTER MIX NEBNext ® High-Fidelity 2X PCR Master Mix is the original non-hot start NEBNext formulation of Q5 ® High-Fidelity DNA Polymerase. This formulation is also included as a component in the original NEBNext standard workflow library prep kits for Illumina, and in NEBNext library prep kits for Ion Torrent. LUNA® UNIVERSAL QPCR MASTER MIX Protocol for Two-step RT-qPCR using the LunaScript® RT SuperMix Kit (NEB #E3010) and the Luna® Universal qPCR Master Mix (NEB #M3003) or Luna Universal Probe qPCR Master Mix (NEB #M3004) Manuals. The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. manualM3003.BSTXI | NEB
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.comQUICK CIP | NEB
Quick CIP is a heat-labile recombinant version of calf intestinal alkaline phosphatase (CIP) purified from a recombinant source. Quick CIP nonspecifically catalyzes the dephosphorylation of 5′ and 3′ ends of DNA and RNA phosphomonoesters. It also hydrolyses ribo-NEBCUTTER V2.0
NEBcutter V2.0. This tool will take a DNA sequence and find the large, non-overlapping open reading frames using the E.coli genetic code and the sites for all Type II and commercially available Type III restriction enzymes that cut the sequence just once. By default, only enzymes available from NEB are used, but other sets may be chosen. MONARCH TOTAL RNA MINIPREP KIT (NEB #T2010) Total RNA was isolated using the Monarch Total RNA Miniprep Kit (NEB #T2010) from varying amounts of HeLa cells over 5 orders of magnitude and eluted in 100 µl of nuclease-free water. Samples were analyzed on a Bioanalyzer Pico chip, with RIN values and total yields shown below the lanes (A). Electropherograms are included as a reference (B). Q5® SITE-DIRECTED MUTAGENESIS KIT Product Information. The Q5 Site-Directed Mutagenesis Kit enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours (Figure 1). The kit utilizes the robust Q5 Hot Start High-Fidelity DNA Polymerase along with custom mutagenic primers to create insertions, deletions and substitutions in a wide variety ofplasmids.
NEBASECHANGER
BASIC WORKFLOW. Working with NEBaseChanger is a 5-step process. Enter the starting plasmid sequence. Choose your mutagenesis experiment. Define the mutation region. (not required for deletions) View primers and annealing REAGENTS FOR THE LIFE SCIENCES INDUSTRY 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.comAGEI | NEB
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com NEBNEXT® HIGH-FIDELITY 2X PCR MASTER MIX NEBNext ® High-Fidelity 2X PCR Master Mix is the original non-hot start NEBNext formulation of Q5 ® High-Fidelity DNA Polymerase. This formulation is also included as a component in the original NEBNext standard workflow library prep kits for Illumina, and in NEBNext library prep kits for Ion Torrent. LUNA® UNIVERSAL QPCR MASTER MIX Protocol for Two-step RT-qPCR using the LunaScript® RT SuperMix Kit (NEB #E3010) and the Luna® Universal qPCR Master Mix (NEB #M3003) or Luna Universal Probe qPCR Master Mix (NEB #M3004) Manuals. The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. manualM3003.BSTXI | NEB
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.comQUICK CIP | NEB
Quick CIP is a heat-labile recombinant version of calf intestinal alkaline phosphatase (CIP) purified from a recombinant source. Quick CIP nonspecifically catalyzes the dephosphorylation of 5′ and 3′ ends of DNA and RNA phosphomonoesters. It also hydrolyses ribo-NEBCUTTER V2.0
NEBcutter V2.0. This tool will take a DNA sequence and find the large, non-overlapping open reading frames using the E.coli genetic code and the sites for all Type II and commercially available Type III restriction enzymes that cut the sequence just once. By default, only enzymes available from NEB are used, but other sets may be chosen. MONARCH TOTAL RNA MINIPREP KIT (NEB #T2010) Total RNA was isolated using the Monarch Total RNA Miniprep Kit (NEB #T2010) from varying amounts of HeLa cells over 5 orders of magnitude and eluted in 100 µl of nuclease-free water. Samples were analyzed on a Bioanalyzer Pico chip, with RIN values and total yields shown below the lanes (A). Electropherograms are included as a reference (B). Q5® SITE-DIRECTED MUTAGENESIS KIT Product Information. The Q5 Site-Directed Mutagenesis Kit enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours (Figure 1). The kit utilizes the robust Q5 Hot Start High-Fidelity DNA Polymerase along with custom mutagenic primers to create insertions, deletions and substitutions in a wide variety ofplasmids.
NEBASECHANGER
BASIC WORKFLOW. Working with NEBaseChanger is a 5-step process. Enter the starting plasmid sequence. Choose your mutagenesis experiment. Define the mutation region. (not required for deletions) View primers and annealingAGEI | NEB
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com NEBNEXT® HIGH-FIDELITY 2X PCR MASTER MIX NEBNext ® High-Fidelity 2X PCR Master Mix is the original non-hot start NEBNext formulation of Q5 ® High-Fidelity DNA Polymerase. This formulation is also included as a component in the original NEBNext standard workflow library prep kits for Illumina, and in NEBNext library prep kits for Ion Torrent. LUNA® UNIVERSAL QPCR MASTER MIX Protocol for Two-step RT-qPCR using the LunaScript® RT SuperMix Kit (NEB #E3010) and the Luna® Universal qPCR Master Mix (NEB #M3003) or Luna Universal Probe qPCR Master Mix (NEB #M3004) Manuals. The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. manualM3003. Q5® SITE-DIRECTED MUTAGENESIS KIT The Q5 Site-Directed Mutagenesis Kit enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours (Figure 1). The kit utilizes the robust Q5 Hot Start High-Fidelity DNA Polymerase along with custom mutagenic primers to create insertions, deletions and substitutions in a wide variety of plasmids.RSRII | NEB
RsrII requires two copies of its recognition sequence for efficient cleavage to occur. If a DNA molecule (plasmid or linear) has only one copy of this recognition site, RsrII cannot digest it to completion.These two copies of recognition sites should ideally be on the same molecule to achieve 100% cleavage, however, two different molecules canbe used with slightly less efficiency.PUBLICATIONS
Are you doing COVID-19 related research? Our latest RUO kit, the Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit, enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes.For simple, visual assay results, the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit includes a color-changing pH indicator for detection of SARS-CoV-2 nucleic acid amplificationVIDEO LIBRARY
Increase Productivity & Reproducibility by Automating DNA Library Prep for Ion Torrent ® Sequencing. Learn how Biomek automation of NEBNext DNA library preparation for Ion Torrent enables high-throughput, reproducible production of libraries with high yield and complexity.PUBLICATIONS
Search publications from New England Biolabs scientists. Are you doing COVID-19 related research? Our latest RUO kit, the Luna ® SARS-CoV-2 RT-qPCR Multiplex Assay Kit, enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes.For simple, visual assay results, the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit includes a color-changing pHENZYME FINDER
Locate commercially available restriction enzymes by category, name, recognition sequence, or overhang. BLUNT/TA LIGASE MASTER MIX Blunt/TA Ligase Master Mix is a ready-to-use solution of T4 DNA Ligase, proprietary ligation enhancer, and optimized reaction buffer. This master mix is specifically formulated to improve ligation and transformation of both blunt-end and single-base overhang substrates. The master mix format simplifies reaction set-up, ensures an optimized YOUR WEB BROWSER (CHROME 69) IS OUT OF DATE. Update your browser for more security, speed and the best experience on this site. Updatebrowser
Ignore
Sign In Sign In or
Sign Up Sign Up
* (0)
* (0)
Welcome, GUEST
* APPLICATIONS & PRODUCTS*
APPLICATIONS
* Cloning & Synthetic Biology * DNA Amplification, PCR and qPCR* Genome Editing
* RNA Analysis
* Sample Prep for NGS & Target Enrichment* Epigenetics
* Protein Expression & Purification * Protein Analysis & Tools * Glycobiology & Proteomics* Cellular Analysis
*
PRODUCT CATEGORIES
* Restriction Endonucleases * PCR, qPCR & Amplification Technologies * DNA Modifying Enzymes * Sample Prep for NGS & Target Enrichment * Nucleic Acid Purification* Markers & Ladders
* RNA Reagents
* DNA Assembly, Cloning and Mutagenesis Kits* Genome Editing
* Cellular Analysis
* Epigenetics
* Protein Expression & Purification Technologies* Competent Cells
* Protein Tools
* Glycobiology
* DNA Plasmids & Substrates* Buffers
* Strains
* Discontinued
New Products
Special Offers
PRODUCT PRICE LIST
Download a PDF containing pricing for our full product list. YOU’LL BE THRILLED TO PIECES Do you need a faster, more reliable solution for DNA fragmentation and library construction? Our new NEBNext® Ultra™ II FS DNA LibraryPrep Kit
with
novel fragmentation reagent meets the dual challenge of generating high quality next gen sequencing libraries from ever-decreasing input amounts AND simple scalability. Learn more and request a sample!* TOOLS & RESOURCES
*
TOOLS
* NEBNext® Selector* NEBcloner®
* NEBioCalculator®
* Thermostable Ligase Reaction Temperature Calculator * NEBuilder®Assembly Tool* Enzyme Finder
* Double Digest Finder* NEBcutter®
* REBASE®
* DNA Sequences and Maps Tool* Tm Calculator
* PCR Fidelity Estimator* PCR Selector
* PolBase®
* NEB Golden Gate Assembly Tool* NEBaseChanger®
* NEBnow Locator
* Read Coverage Calculator * Competitor Cross Reference*
RESOURCES
* FAQs
* Protocols
* Selection Charts
* Troubleshooting Guides * Usage Guidelines/Tips* Interactive Tools
* Video Library
* Web Tools
* Webinars
* Application Notes
Order Catalog
FREE SHIPPING
SAVE TIME and money by placing an order with NEB. Take advantage of free shipping for any order totaling over $350. Place your order before 8pm EST for overnight delivery.* SUPPORT
* Technical Support
* US Customer & Order Support * International Ordering & Support * Customer Suggestions & Feedback * Find My Local US Sales Representative * New Lab/BioTech Discount * Catalog & Request Literature * NEBnow® Freezer Program * NEBsolutions® / OEM* Biotech Support
* Student Services & Materials * Packaging & Shipping* Quality Assurance
Customer Suggestions & FeedbackTechnical Support
CONTACT INFORMATION
240 County Road
Ipswich, MA 01938-2723978-927-5054
(Toll Free) 1-800-632-5227Fax: 978-921-1350
Info@neb.com
* ABOUT
* NEB Overview
* News & Press Releases* Leadership
* Research at NEB
* Environmental Commitment* Quality at NEB
* ISO Certification
* Passion in Science Award * Business Development Opportunities* Careers
* Contact Us
CONTACT INFORMATION
240 County Road
Ipswich, MA 01938-2723978-927-5054
(Toll Free) 1-800-632-5227Fax: 978-921-1350
Info@neb.com * Quick OrderSearch
Quick Order
__ | __ | __
* APPLICATIONS & PRODUCTS*
APPLICATIONS
* Cloning & Synthetic Biology * DNA Amplification, PCR and qPCR* Genome Editing
* RNA Analysis
* Sample Prep for NGS & Target Enrichment* Epigenetics
* Protein Expression & Purification * Protein Analysis & Tools * Glycobiology & Proteomics* Cellular Analysis
*
PRODUCT CATEGORIES
* Restriction Endonucleases * PCR, qPCR & Amplification Technologies * DNA Modifying Enzymes * Sample Prep for NGS & Target Enrichment * Nucleic Acid Purification* Markers & Ladders
* RNA Reagents
* DNA Assembly, Cloning and Mutagenesis Kits* Genome Editing
* Cellular Analysis
* Epigenetics
* Protein Expression & Purification Technologies* Competent Cells
* Protein Tools
* Glycobiology
* DNA Plasmids & Substrates* Buffers
* Strains
* Discontinued
New Products
Special Offers
PRODUCT PRICE LIST
Download a PDF containing pricing for our full product list. YOU’LL BE THRILLED TO PIECES Do you need a faster, more reliable solution for DNA fragmentation and library construction? Our new NEBNext® Ultra™ II FS DNA LibraryPrep Kit
with
novel fragmentation reagent meets the dual challenge of generating high quality next gen sequencing libraries from ever-decreasing input amounts AND simple scalability. Learn more and request a sample!* TOOLS & RESOURCES
*
TOOLS
* NEBNext® Selector* NEBcloner®
* NEBioCalculator®
* Thermostable Ligase Reaction Temperature Calculator * NEBuilder®Assembly Tool* Enzyme Finder
* Double Digest Finder* NEBcutter®
* REBASE®
* DNA Sequences and Maps Tool* Tm Calculator
* PCR Fidelity Estimator* PCR Selector
* PolBase®
* NEB Golden Gate Assembly Tool* NEBaseChanger®
* NEBnow Locator
* Read Coverage Calculator * Competitor Cross Reference*
RESOURCES
* FAQs
* Protocols
* Selection Charts
* Troubleshooting Guides * Usage Guidelines/Tips* Interactive Tools
* Video Library
* Web Tools
* Webinars
* Application Notes
Order Catalog
FREE SHIPPING
SAVE TIME and money by placing an order with NEB. Take advantage of free shipping for any order totaling over $350. Place your order before 8pm EST for overnight delivery.* SUPPORT
* Technical Support
* US Customer & Order Support * International Ordering & Support * Customer Suggestions & Feedback * Find My Local US Sales Representative * New Lab/BioTech Discount * Catalog & Request Literature * NEBnow® Freezer Program * NEBsolutions® / OEM* Biotech Support
* Student Services & Materials * Packaging & Shipping* Quality Assurance
Customer Suggestions & FeedbackTechnical Support
CONTACT INFORMATION
240 County Road
Ipswich, MA 01938-2723978-927-5054
(Toll Free) 1-800-632-5227Fax: 978-921-1350
Info@neb.com
* ABOUT
* NEB Overview
* News & Press Releases* Leadership
* Research at NEB
* Environmental Commitment* Quality at NEB
* ISO Certification
* Passion in Science Award * Business Development Opportunities* Careers
* Contact Us
CONTACT INFORMATION
240 County Road
Ipswich, MA 01938-2723978-927-5054
(Toll Free) 1-800-632-5227Fax: 978-921-1350
Info@neb.com * Quick Order* Sign In
* Sign Up
*
PRACTICING RESPONSIBLE SCIENCE FROM PLACING THE ADVANCEMENT OF SCIENCE AND THE STEWARDSHIP OF THE ENVIRONMENT AS OUR HIGHEST PRIORITIES, TO OUR UNIQUE CORPORATE CULTURE, NEB'S PHILOSOPHY CAN BE DISTILLED DOWN TO THREE CORE VALUES: PASSION, HUMILITY, AND BEING GENUINE.Read More
Need Help?Find the support you need Making a Purchase? Browse products or quick order Latest ResearchReview NEB’s latest research What's TrendingFind out what's new at NEB ------------------------- Find the Support You NeedLITERATURE
Browse our selection of literature and request your copy of the NEB Catalog and Technical Reference. View or Request LiteratureTOOLS
Explore our selection of helpful online tools for your research and experimental design.Browse All Tools
VIDEO LIBRARY
Visit our video library to find tutorials, protocols and other informative content.View All Videos
PROTOCOL SEARCH
Search from over 1,000 NEB protocols within our database. __ View All Protocols ------------------------- Find the Product You NeedREADY TO ORDER?
Try our Quick Order feature for faster ordering of products. Quick Order Browse All Products SOMETHING TO CHEW ON. NEB’S EXONUCLEASES AND NON-SPECIFIC ENDONUCLEASES ARE OPTIMIZED FOR ROBUST PERFORMANCE IN STREAMLINED WORKFLOWS. INTRODUCING THE MONARCH® GENOMIC DNA PURIFICATION KIT Purify high quality, high molecular weight gDNA from multiple sample types, all with one kit!Request a sample
NEBNEXT® GLOBIN & RRNA DEPLETION KIT (HUMAN/MOUSE/RAT) This new kit employs the NEBNext RNase H-based RNA depletion workflow to deplete globin mRNA, cytoplasmic rRNA and mitochondrial rRNA with human, mouse and rat samples, and is effective with intact anddegraded RNA.
Learn More
Q5U® HOT START HIGH-FIDELITY DNA POLYMERASE Amplify templates containing uracil and inosine bases with this modified, thermostable version of Q5® High-Fidelity DNA Polymerase.Learn More
Browse All Products
------------------------- Review NEB’s Latest ResearchTECHNICAL NOTE
Learn about NEBNext Enzymatic Methyl-seq (EM-seq), a high-performance, enzyme-based alternative to bisulfite sequencing for methylomeanalysis.
APPLICATION NOTE
Access a detailed guide for analyzing nucleic acid concentration and purity with microvolume spectrophotometers.FEATURE ARTICLE
In this article, we explore the effect of nucleic acid modification on digestion by DNA exonucleases.NEB PUBLICATION
Learn more about a highly sensitive loop-mediated isothermal amplification (LAMP) diagnostic test to detect _mansonelliasis_.LEARN MORE
Learn about RADAR-seq, a rare damage and repair sequencing method for detecting DNA damage on a genome-wide scale.RESEARCH SPOTLIGHT
Our Enzymes for Innovation initiative aims to find novel enzymes with unique activities for manipulating DNA, RNA, proteins and glycans. Learn About NEB's Research -------------------------Trending Topics
Featured Podcast
Interview with 2019 Passion in Science Awardees: Global STEM EducationPrograms
Featured Podcast
Interview with 2019 Passion in Science Awardees: Global STEM EducationPrograms
Hear from the founders of the STEM programs BioEYES, SciComNigeria, and Xenoplex STEM Center about how they are connecting students and communities with science around the world. Listen to the PodcastFeatured Video
Advances in Methylome AnalysisFeatured Video
Advances in Methylome Analysis Learn about the challenges associated with current methods available for methylome analysis, and how NEBNext Enzymatic Methyl-seq (EM-seq™), our new alternative to bisulfite sequencing, addressesthese challenges.
View the Video
Featured Webinar
Exonucleases and Endonucleases as Molecular ToolsFeatured Webinar
Exonucleases and Endonucleases as Molecular Tools Review the activities of exonucleases and endonucleases, get insight on how to choose the right exo- or endo- for various molecular biology applications, and learn how to use these reagents when developing new molecular biology workflows.Watch the Webinar
Social
-------------------------Follow Us
Upcoming Events
-------------------------Oct
1
SYNBIOBETA
San Francisco, CA | October 1-3Oct
15
ASHG
Houston, TX | October 15-19Nov
7
AMP
Baltimore, MD | November 7-9Dec
7
ASCB
Washington, DC | December 7-11Jan
12
PAG
San Diego, CA | January 12-16View All Events
TO REQUEST TECHNICAL SUPPORT Fill out our Technical Support Form , email us, or call 1-800-632-7799. FOR HELP WITH YOUR ORDER Contact our Customer Service Team by email or call 1-800-NEB-LABS.GET IN TOUCH
Customer Support Technical SupportReach Out
SIGN UP AND SELECT NEB EMAIL NEWSLETTERS TARGETED TO YOUR RESEARCHINGSelect Newsletters
Support NEB Overview Careers Site Map Terms of Use Trademarks Terms of SalePrivacy
*
*
*
*
*
Copyright 2019 New England Biolabs. All Rights Reserved.Session Expired
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.Sign In
Institution Changed
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.Sign In
Sign in to your NEB account To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.Sign In Sign In
Continue as Guest
Don't show me again
Details
5