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ZFIN GENE: RTN2B
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate.ZFIN GENE: HMGA1A
hmga1a. Predicted to have transcription coregulator activity. Involved in regulation of RNA splicing. Predicted to localize to nucleus. Is expressed in DEL; central nervous system; heart; margin; and otic vesicle. Human ortholog (s) of this gene implicated in type 2 diabetes mellitus. Orthologous to human HMGA1 (high mobility group AT-hook 1).ZFIN GENE: TGFB1A
tgfb1a ID ZDB-GENE-030618-1 Name transforming growth factor, beta 1a Symbol tgfb1a Nomenclature History Previous Names. tgfb1 (); ai39657; wu:fb13a07 (); xx:ai39657; Type protein_coding_geneZFIN GENE: PDYN
Predicted to be involved in chemical synaptic transmission; neuropeptide signaling pathway; and sensory perception. Predicted to localize to several cellular components, including axon terminus; dendrite; and neuronal cell body. Is expressed in brain and hypothalamus. Human ortholog (s) of this gene implicated in spinocerebellar ataxia type 23ZFIN GENE: ACTB2
actb2. Predicted to be a structural constituent of postsynaptic actin cytoskeleton. Predicted to localize to several cellular components, including actin filament; dense body; and focal adhesion. Is expressed in several structures, including blastomere; brain; eye; musculature system; and pleuroperitoneal region.ZFIN GENE: SETA
seta ID ZDB-GENE-030131-2221 Name SET nuclear proto-oncogene a Symbol seta Nomenclature History Previous Names. ik:tdsubc_2c6; wu:fb99h10; xx:tdsubc_2c6; Type protein_coding_geneZFIN GENE: RAF1A
raf1a ID ZDB-GENE-990415-41 Name Raf-1 proto-oncogene, serine/threonine kinase a Symbol raf1a Nomenclature History Previous Names. craf; raf1; Type protein_coding_gene LocationZFIN GENE: OC90
oc90 ID ZDB-GENE-070912-300 Name otoconin 90 Symbol oc90 Nomenclature History Previous Names. otoc1 (); si:ch211-66b9.2; wu:fb30b04; Type protein_coding_gene Location Chr: 2 Mapping Details/BrowsersDescription
ZFIN GENE: SLC20A1A
slc20a1a ID ZDB-GENE-040426-2217 Name solute carrier family 20 member 1a Symbol slc20a1a Nomenclature History Previous Names. sb:cb1011; zgc:85672; Type protein_coding_gene ZFIN THE ZEBRAFISH INFORMATION NETWORKFIGURE 2 OF FALLATA ET AL 2019ZFIN WARRANTY DISCLAIMERGLOSSARYFIG. 3 OF BAGWELL ET AL 2020 ZFIN is hiring both a software developer and a curator! The Zebrafish Information Network (ZFIN) is the database of genetic and genomic data for the zebrafish (Danio rerio) as a model organism.ZFIN provides a wide array of expertly curated, organized and cross-referenced zebrafish research data.ZFIN GENE: RTN2B
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate.ZFIN GENE: HMGA1A
hmga1a. Predicted to have transcription coregulator activity. Involved in regulation of RNA splicing. Predicted to localize to nucleus. Is expressed in DEL; central nervous system; heart; margin; and otic vesicle. Human ortholog (s) of this gene implicated in type 2 diabetes mellitus. Orthologous to human HMGA1 (high mobility group AT-hook 1).ZFIN GENE: TGFB1A
tgfb1a ID ZDB-GENE-030618-1 Name transforming growth factor, beta 1a Symbol tgfb1a Nomenclature History Previous Names. tgfb1 (); ai39657; wu:fb13a07 (); xx:ai39657; Type protein_coding_geneZFIN GENE: PDYN
Predicted to be involved in chemical synaptic transmission; neuropeptide signaling pathway; and sensory perception. Predicted to localize to several cellular components, including axon terminus; dendrite; and neuronal cell body. Is expressed in brain and hypothalamus. Human ortholog (s) of this gene implicated in spinocerebellar ataxia type 23ZFIN GENE: ACTB2
actb2. Predicted to be a structural constituent of postsynaptic actin cytoskeleton. Predicted to localize to several cellular components, including actin filament; dense body; and focal adhesion. Is expressed in several structures, including blastomere; brain; eye; musculature system; and pleuroperitoneal region.ZFIN GENE: SETA
seta ID ZDB-GENE-030131-2221 Name SET nuclear proto-oncogene a Symbol seta Nomenclature History Previous Names. ik:tdsubc_2c6; wu:fb99h10; xx:tdsubc_2c6; Type protein_coding_geneZFIN GENE: RAF1A
raf1a ID ZDB-GENE-990415-41 Name Raf-1 proto-oncogene, serine/threonine kinase a Symbol raf1a Nomenclature History Previous Names. craf; raf1; Type protein_coding_gene LocationZFIN GENE: OC90
oc90 ID ZDB-GENE-070912-300 Name otoconin 90 Symbol oc90 Nomenclature History Previous Names. otoc1 (); si:ch211-66b9.2; wu:fb30b04; Type protein_coding_gene Location Chr: 2 Mapping Details/BrowsersDescription
ZFIN GENE: SLC20A1A
slc20a1a ID ZDB-GENE-040426-2217 Name solute carrier family 20 member 1a Symbol slc20a1a Nomenclature History Previous Names. sb:cb1011; zgc:85672; Type protein_coding_geneZFIN GENE: KDRL
kdrl. Exhibits protein tyrosine kinase activity. Involved in circulatory system development and thyroid gland development. Predicted to localize to integral component of plasma membrane and receptor complex. Is expressed in several structures, including cardiovascular system; head; hematopoietic system; mesoderm; and pleuroperitoneal region. ZFIN: ZEBRAFISH BOOK: GENERAL METHODS Use about 10 drops 1 M NaOH to Ph 7.2 Hank's solutions: Hank's solutions can be made from stock solutions (kept refrigerated, they will last for several months). A premix of the salts can be stored in the refrigerator for several weeks. Sodium bicarbonate does not store well, so it is made up fresh each time Hank's solution is made.ZFIN GENE: ALDOCA
aldoca. Predicted to have fructose-bisphosphate aldolase activity. Predicted to be involved in fructose 1,6-bisphosphate metabolic process and glycolytic process. Predicted to localize to cytosol. Is expressed in brain. Human ortholog (s) of this gene implicated in autoimmune disease of the nervous system. ZFIN PUBLICATION: CI ET AL., 2020 Epilepsy is the fourth most common neurological disorder, and aberrantly elevated sulfur dioxide derivatives (SO 3 2-/HSO 3-) are thought to underlie the hippocampal neuronal apoptosis in epilepsy.We have designed and synthesized a mitochondria-targeted polydopamine nanoprobe for visualizing endogenous SO 3 2-/HSO 3-by the nucleophilicaddition reaction.
ZFIN PUBLICATION: LI ET AL., 2020 Date: 2020: Source: Chemosphere 256: 127105 (Journal) Generate reference Registered Authors: Keywords: ZFIN PUBLICATION: JOHANSEN ET AL., 2020 Johansen, M.D., Kremer, L. (2020) A zebrafish model of Mycobacterium kansasii infection reveals large extracellular cord formation. The Journal of infectious diseases. 222(6):1046-1050.ZFIN GENE: UBB
ubb. Predicted to have protein tag and ubiquitin protein ligase binding activity. Predicted to be involved in modification-dependent protein catabolic process and protein ubiquitination. Predicted to localize to cytosolic small ribosomal subunit and nucleus. Orthologousto
ZFIN GENE: WNT1
wnt1 ID ZDB-GENE-980526-526 Name wingless-type MMTV integration site family, member 1 Symbol wnt1 Nomenclature History Previous Names. int-1; etID22400.23 (); sb:eu647; zgc:194464 ZFIN ANTIBODY: AB1-ENKEPHALIN Comment Citation; This antibody was generated by immunization of rabbits with Met-Enkephalin ZFIN Curated Data ZFIN PUBLICATION: URASAKI ET AL., 2019: CRISPR LIST Urasaki et al., 2019 - Shootins mediate collective cell migration and organogenesis of the zebrafish posterior lateral line system. Scientific Reports 9:12156 Full text @ Sci. Rep. ZFIN THE ZEBRAFISH INFORMATION NETWORKFIGURE 2 OF FALLATA ET AL 2019ZFIN WARRANTY DISCLAIMERGLOSSARYFIG. 3 OF BAGWELL ET AL 2020 ZFIN is hiring both a software developer and a curator! The Zebrafish Information Network (ZFIN) is the database of genetic and genomic data for the zebrafish (Danio rerio) as a model organism.ZFIN provides a wide array of expertly curated, organized and cross-referenced zebrafish research data.ZFIN GENE: TGFB1A
tgfb1a ID ZDB-GENE-030618-1 Name transforming growth factor, beta 1a Symbol tgfb1a Nomenclature History Previous Names. tgfb1 (); ai39657; wu:fb13a07 (); xx:ai39657; Type protein_coding_gene ZFIN: ZEBRAFISH BOOK: GENERAL METHODS Use about 10 drops 1 M NaOH to Ph 7.2 Hank's solutions: Hank's solutions can be made from stock solutions (kept refrigerated, they will last for several months). A premix of the salts can be stored in the refrigerator for several weeks. Sodium bicarbonate does not store well, so it is made up fresh each time Hank's solution is made.ZFIN GENE: RTN2B
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate.ZFIN GENE: HMGA1A
hmga1a. Predicted to have transcription coregulator activity. Involved in regulation of RNA splicing. Predicted to localize to nucleus. Is expressed in DEL; central nervous system; heart; margin; and otic vesicle. Human ortholog (s) of this gene implicated in type 2 diabetes mellitus. Orthologous to human HMGA1 (high mobility group AT-hook 1).ZFIN GENE: KDRL
kdrl. Exhibits protein tyrosine kinase activity. Involved in circulatory system development and thyroid gland development. Predicted to localize to integral component of plasma membrane and receptor complex. Is expressed in several structures, including cardiovascular system; head; hematopoietic system; mesoderm; and pleuroperitoneal region.ZFIN GENE: TFA
tfa. Predicted to have metal ion binding activity. Involved in hemoglobin biosynthetic process; iron ion transport; and response to bacterium. Predicted to localize to endosome; extracellular space; and plasma membrane. Is expressed in several structures, including ball;ZFIN GENE: ACTC1B
actc1b. Predicted to localize to dynactin complex. Is expressed in several structures, including EVL; mesoderm; musculature system; pericardial region; and trunk. Used to study nemaline myopathy. Human ortholog (s) of this gene implicated in atrial heart septal defect 5; dilated cardiomyopathy; dilated cardiomyopathy 1R; and hypertrophicZFIN GENE: SETA
seta ID ZDB-GENE-030131-2221 Name SET nuclear proto-oncogene a Symbol seta Nomenclature History Previous Names. ik:tdsubc_2c6; wu:fb99h10; xx:tdsubc_2c6; Type protein_coding_geneZFIN GENE: JAG2B
jag2b. Predicted to have Notch binding activity and calcium ion binding activity. Involved in animal organ development. Predicted to localize to plasma membrane and protein-containing complex. Is expressed in several structures, including anterior neural rod; digestive system; mesoderm; nervous system; and trunk. ZFIN THE ZEBRAFISH INFORMATION NETWORKFIGURE 2 OF FALLATA ET AL 2019ZFIN WARRANTY DISCLAIMERGLOSSARYFIG. 3 OF BAGWELL ET AL 2020 ZFIN is hiring both a software developer and a curator! The Zebrafish Information Network (ZFIN) is the database of genetic and genomic data for the zebrafish (Danio rerio) as a model organism.ZFIN provides a wide array of expertly curated, organized and cross-referenced zebrafish research data.ZFIN GENE: TGFB1A
tgfb1a ID ZDB-GENE-030618-1 Name transforming growth factor, beta 1a Symbol tgfb1a Nomenclature History Previous Names. tgfb1 (); ai39657; wu:fb13a07 (); xx:ai39657; Type protein_coding_gene ZFIN: ZEBRAFISH BOOK: GENERAL METHODS Use about 10 drops 1 M NaOH to Ph 7.2 Hank's solutions: Hank's solutions can be made from stock solutions (kept refrigerated, they will last for several months). A premix of the salts can be stored in the refrigerator for several weeks. Sodium bicarbonate does not store well, so it is made up fresh each time Hank's solution is made.ZFIN GENE: RTN2B
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate.ZFIN GENE: HMGA1A
hmga1a. Predicted to have transcription coregulator activity. Involved in regulation of RNA splicing. Predicted to localize to nucleus. Is expressed in DEL; central nervous system; heart; margin; and otic vesicle. Human ortholog (s) of this gene implicated in type 2 diabetes mellitus. Orthologous to human HMGA1 (high mobility group AT-hook 1).ZFIN GENE: KDRL
kdrl. Exhibits protein tyrosine kinase activity. Involved in circulatory system development and thyroid gland development. Predicted to localize to integral component of plasma membrane and receptor complex. Is expressed in several structures, including cardiovascular system; head; hematopoietic system; mesoderm; and pleuroperitoneal region.ZFIN GENE: TFA
tfa. Predicted to have metal ion binding activity. Involved in hemoglobin biosynthetic process; iron ion transport; and response to bacterium. Predicted to localize to endosome; extracellular space; and plasma membrane. Is expressed in several structures, including ball;ZFIN GENE: ACTC1B
actc1b. Predicted to localize to dynactin complex. Is expressed in several structures, including EVL; mesoderm; musculature system; pericardial region; and trunk. Used to study nemaline myopathy. Human ortholog (s) of this gene implicated in atrial heart septal defect 5; dilated cardiomyopathy; dilated cardiomyopathy 1R; and hypertrophicZFIN GENE: SETA
seta ID ZDB-GENE-030131-2221 Name SET nuclear proto-oncogene a Symbol seta Nomenclature History Previous Names. ik:tdsubc_2c6; wu:fb99h10; xx:tdsubc_2c6; Type protein_coding_geneZFIN GENE: JAG2B
jag2b. Predicted to have Notch binding activity and calcium ion binding activity. Involved in animal organ development. Predicted to localize to plasma membrane and protein-containing complex. Is expressed in several structures, including anterior neural rod; digestive system; mesoderm; nervous system; and trunk. ZFIN ZEBRAFISH DEVELOPMENTAL STAGES Embryonic shield visible from animal pole; 50%-epiboly. Browse AO. 75%-epiboly. 8.00 h. Dorsal side distinctly thicker; epiblast, hypoblast, evacuation zone visible. Browse AO. 90%-epiboly. 9.00 h. Axis and neural plate; brain and notochord rudiments. ZFIN PUBLICATION: LI ET AL., 2020 Date: 2020: Source: Chemosphere 256: 127105 (Journal) Generate reference Registered Authors: Keywords: ZFIN PUBLICATION: CI ET AL., 2020 Epilepsy is the fourth most common neurological disorder, and aberrantly elevated sulfur dioxide derivatives (SO 3 2-/HSO 3-) are thought to underlie the hippocampal neuronal apoptosis in epilepsy.We have designed and synthesized a mitochondria-targeted polydopamine nanoprobe for visualizing endogenous SO 3 2-/HSO 3-by the nucleophilicaddition reaction.
ZFIN PUBLICATION: JOHANSEN ET AL., 2020 Johansen, M.D., Kremer, L. (2020) A zebrafish model of Mycobacterium kansasii infection reveals large extracellular cord formation. The Journal of infectious diseases. 222(6):1046-1050.ZFIN GENE: RTN2B
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate.ZFIN GENE: ACTB2
actb2. Predicted to be a structural constituent of postsynaptic actin cytoskeleton. Predicted to localize to several cellular components, including actin filament; dense body; and focal adhesion. Is expressed in several structures, including blastomere; brain; eye; musculature system; and pleuroperitoneal region. ZFIN PUBLICATION: HAMON ET AL., 2020 In addition to the already described ligand L4a, two pyclen based lanthanide chelators, L4b and L4c, bearing two specific picolinate two-photon antennas (tailor-made for each targeted metal) and one acetate arm arranged in a dissymmetrical manner, have been synthesized, to form a complete family of lanthanide luminescent bioprobes: , , , and .ZFIN GENE: FABP10A
Description. Exhibits bile acid binding activity. Predicted to localize to cytoplasm. Is expressed in several structures, including eye; female organism; heart; liver; and pleuroperitoneal region.ZFIN GENE: ALCAMA
alcama. Exhibits identical protein binding activity. Involved in several processes, including animal organ morphogenesis; neural crest cell differentiation; and positive regulation of cell morphogenesis involved in differentiation. Localizes to axon.ZFIN GENE: SPG11
spg11 ID ZDB-GENE-101017-1 Name SPG11 vesicle trafficking associated, spatacsin Symbol spg11 Nomenclature History Previous Names. spatacsin (); Type protein_coding_gene Location ZFIN THE ZEBRAFISH INFORMATION NETWORKFIGURE 2 OF FALLATA ET AL 2019ZFIN WARRANTY DISCLAIMERGLOSSARYFIG. 3 OF BAGWELL ET AL 2020 ZFIN is hiring both a software developer and a curator! The Zebrafish Information Network (ZFIN) is the database of genetic and genomic data for the zebrafish (Danio rerio) as a model organism.ZFIN provides a wide array of expertly curated, organized and cross-referenced zebrafish research data.ZFIN GENE: HMGA1A
hmga1a. Predicted to have transcription coregulator activity. Involved in regulation of RNA splicing. Predicted to localize to nucleus. Is expressed in DEL; central nervous system; heart; margin; and otic vesicle. Human ortholog (s) of this gene implicated in type 2 diabetes mellitus. Orthologous to human HMGA1 (high mobility group AT-hook 1).ZFIN GENE: VSX2
Involved in negative regulation of transcription by RNA polymerase II and regulation of neural retina development. Predicted to localize to nucleus. Is expressed in nervous system; neural tube; optic cup; and optic vesicle. Human ortholog (s) of this gene implicated in blindness and isolated microphthalmia 2.ZFIN GENE: TGFB1A
tgfb1a ID ZDB-GENE-030618-1 Name transforming growth factor, beta 1a Symbol tgfb1a Nomenclature History Previous Names. tgfb1 (); ai39657; wu:fb13a07 (); xx:ai39657; Type protein_coding_geneZFIN GENE: HE1.1
Description. Predicted to have metalloendopeptidase activity. Predicted to be involved in proteolysis. Is expressed in hatching gland and polster. Orthologous to human ASTL (astacin like metalloendopeptidase). Genome Resources. Alliance ( 1) Gene:569018 ( 1) VEGA:OTTDARG00000009891 ( 1)ZFIN GENE: SETA
seta ID ZDB-GENE-030131-2221 Name SET nuclear proto-oncogene a Symbol seta Nomenclature History Previous Names. ik:tdsubc_2c6; wu:fb99h10; xx:tdsubc_2c6; Type protein_coding_geneZFIN GENE: KCNA5
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate.ZFIN GENE: ANXA4
anxa4 ID ZDB-GENE-030707-1 Name annexin A4 Symbol anxa4 Nomenclature History Previous Names None Type protein_coding_gene Location Chr: 10 Mapping Details/Browsers DescriptionZFIN GENE: SPG11
spg11 ID ZDB-GENE-101017-1 Name SPG11 vesicle trafficking associated, spatacsin Symbol spg11 Nomenclature History Previous Names. spatacsin (); Type protein_coding_gene LocationZFIN GENE: OC90
oc90 ID ZDB-GENE-070912-300 Name otoconin 90 Symbol oc90 Nomenclature History Previous Names. otoc1 (); si:ch211-66b9.2; wu:fb30b04; Type protein_coding_gene Location Chr: 2 Mapping Details/BrowsersDescription
ZFIN THE ZEBRAFISH INFORMATION NETWORKFIGURE 2 OF FALLATA ET AL 2019ZFIN WARRANTY DISCLAIMERGLOSSARYFIG. 3 OF BAGWELL ET AL 2020 ZFIN is hiring both a software developer and a curator! The Zebrafish Information Network (ZFIN) is the database of genetic and genomic data for the zebrafish (Danio rerio) as a model organism.ZFIN provides a wide array of expertly curated, organized and cross-referenced zebrafish research data.ZFIN GENE: HMGA1A
hmga1a. Predicted to have transcription coregulator activity. Involved in regulation of RNA splicing. Predicted to localize to nucleus. Is expressed in DEL; central nervous system; heart; margin; and otic vesicle. Human ortholog (s) of this gene implicated in type 2 diabetes mellitus. Orthologous to human HMGA1 (high mobility group AT-hook 1).ZFIN GENE: VSX2
Involved in negative regulation of transcription by RNA polymerase II and regulation of neural retina development. Predicted to localize to nucleus. Is expressed in nervous system; neural tube; optic cup; and optic vesicle. Human ortholog (s) of this gene implicated in blindness and isolated microphthalmia 2.ZFIN GENE: TGFB1A
tgfb1a ID ZDB-GENE-030618-1 Name transforming growth factor, beta 1a Symbol tgfb1a Nomenclature History Previous Names. tgfb1 (); ai39657; wu:fb13a07 (); xx:ai39657; Type protein_coding_geneZFIN GENE: HE1.1
Description. Predicted to have metalloendopeptidase activity. Predicted to be involved in proteolysis. Is expressed in hatching gland and polster. Orthologous to human ASTL (astacin like metalloendopeptidase). Genome Resources. Alliance ( 1) Gene:569018 ( 1) VEGA:OTTDARG00000009891 ( 1)ZFIN GENE: SETA
seta ID ZDB-GENE-030131-2221 Name SET nuclear proto-oncogene a Symbol seta Nomenclature History Previous Names. ik:tdsubc_2c6; wu:fb99h10; xx:tdsubc_2c6; Type protein_coding_geneZFIN GENE: KCNA5
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate.ZFIN GENE: ANXA4
anxa4 ID ZDB-GENE-030707-1 Name annexin A4 Symbol anxa4 Nomenclature History Previous Names None Type protein_coding_gene Location Chr: 10 Mapping Details/Browsers DescriptionZFIN GENE: SPG11
spg11 ID ZDB-GENE-101017-1 Name SPG11 vesicle trafficking associated, spatacsin Symbol spg11 Nomenclature History Previous Names. spatacsin (); Type protein_coding_gene LocationZFIN GENE: OC90
oc90 ID ZDB-GENE-070912-300 Name otoconin 90 Symbol oc90 Nomenclature History Previous Names. otoc1 (); si:ch211-66b9.2; wu:fb30b04; Type protein_coding_gene Location Chr: 2 Mapping Details/BrowsersDescription
ZFIN GENE: RTN2B
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. ZFIN PUBLICATION: HAMON ET AL., 2020 In addition to the already described ligand L4a, two pyclen based lanthanide chelators, L4b and L4c, bearing two specific picolinate two-photon antennas (tailor-made for each targeted metal) and one acetate arm arranged in a dissymmetrical manner, have been synthesized, to form a complete family of lanthanide luminescent bioprobes: , , , and .ZFIN GENE: ACTB2
actb2. Predicted to be a structural constituent of postsynaptic actin cytoskeleton. Predicted to localize to several cellular components, including actin filament; dense body; and focal adhesion. Is expressed in several structures, including blastomere; brain; eye; musculature system; and pleuroperitoneal region. ZFIN: ZEBRAFISH BOOK: GENERAL METHODS Use about 10 drops 1 M NaOH to Ph 7.2 Hank's solutions: Hank's solutions can be made from stock solutions (kept refrigerated, they will last for several months). A premix of the salts can be stored in the refrigerator for several weeks. Sodium bicarbonate does not store well, so it is made up fresh each time Hank's solution is made. ZFIN PUBLICATION: BOYLE ET AL., 2020 Plastic polymers such as polyvinyl chloride (PVC) may contain chemical additives, such as lead (Pb), that are leachable in aqueous solution. The fragmentation into microplastics (MPs) of plastics such as PVC may facilitate desorption of chemical additives and increase exposure ofaquatic animals.
ZFIN PUBLICATION: CHEN ET AL., 2020 Date: 2020: Source: Journal of Agricultural and Food Chemistry 68(40): 11170-11181 (Journal) Generate reference Registered Authors: ZFIN GENE: ZMP:0000001237 We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate.ZFIN GENE: SPG11
spg11 ID ZDB-GENE-101017-1 Name SPG11 vesicle trafficking associated, spatacsin Symbol spg11 Nomenclature History Previous Names. spatacsin (); Type protein_coding_gene Location ZFIN: ZEBRAFISH BOOK: CONTENTS This book is dedicated to the late George Streisinger. who first began the study of zebrafish in Eugene and. whose insight and gentle support got us all started. Adapted for the Web by ZFIN. Please address corrections and additions to: Monte Westerfield, Institute ofZFIN GENE: WNT1
wnt1 ID ZDB-GENE-980526-526 Name wingless-type MMTV integration site family, member 1 Symbol wnt1 Nomenclature History Previous Names. int-1; etID22400.23 (); sb:eu647; zgc:194464 ZFIN THE ZEBRAFISH INFORMATION NETWORKFIGURE 2 OF FALLATA ET AL 2019ZFIN WARRANTY DISCLAIMERGLOSSARYFIG. 3 OF BAGWELL ET AL 2020 ZFIN is hiring both a software developer and a curator! The Zebrafish Information Network (ZFIN) is the database of genetic and genomic data for the zebrafish (Danio rerio) as a model organism.ZFIN provides a wide array of expertly curated, organized and cross-referenced zebrafish research data.ZFIN GENE: HMGA1A
hmga1a. Predicted to have transcription coregulator activity. Involved in regulation of RNA splicing. Predicted to localize to nucleus. Is expressed in DEL; central nervous system; heart; margin; and otic vesicle. Human ortholog (s) of this gene implicated in type 2 diabetes mellitus. Orthologous to human HMGA1 (high mobility group AT-hook 1).ZFIN GENE: VSX2
Involved in negative regulation of transcription by RNA polymerase II and regulation of neural retina development. Predicted to localize to nucleus. Is expressed in nervous system; neural tube; optic cup; and optic vesicle. Human ortholog (s) of this gene implicated in blindness and isolated microphthalmia 2.ZFIN GENE: TGFB1A
tgfb1a ID ZDB-GENE-030618-1 Name transforming growth factor, beta 1a Symbol tgfb1a Nomenclature History Previous Names. tgfb1 (); ai39657; wu:fb13a07 (); xx:ai39657; Type protein_coding_geneZFIN GENE: HE1.1
Description. Predicted to have metalloendopeptidase activity. Predicted to be involved in proteolysis. Is expressed in hatching gland and polster. Orthologous to human ASTL (astacin like metalloendopeptidase). Genome Resources. Alliance ( 1) Gene:569018 ( 1) VEGA:OTTDARG00000009891 ( 1)ZFIN GENE: SETA
seta ID ZDB-GENE-030131-2221 Name SET nuclear proto-oncogene a Symbol seta Nomenclature History Previous Names. ik:tdsubc_2c6; wu:fb99h10; xx:tdsubc_2c6; Type protein_coding_geneZFIN GENE: KCNA5
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate.ZFIN GENE: ANXA4
anxa4 ID ZDB-GENE-030707-1 Name annexin A4 Symbol anxa4 Nomenclature History Previous Names None Type protein_coding_gene Location Chr: 10 Mapping Details/Browsers DescriptionZFIN GENE: SPG11
spg11 ID ZDB-GENE-101017-1 Name SPG11 vesicle trafficking associated, spatacsin Symbol spg11 Nomenclature History Previous Names. spatacsin (); Type protein_coding_gene LocationZFIN GENE: OC90
oc90 ID ZDB-GENE-070912-300 Name otoconin 90 Symbol oc90 Nomenclature History Previous Names. otoc1 (); si:ch211-66b9.2; wu:fb30b04; Type protein_coding_gene Location Chr: 2 Mapping Details/BrowsersDescription
ZFIN THE ZEBRAFISH INFORMATION NETWORKFIGURE 2 OF FALLATA ET AL 2019ZFIN WARRANTY DISCLAIMERGLOSSARYFIG. 3 OF BAGWELL ET AL 2020 ZFIN is hiring both a software developer and a curator! The Zebrafish Information Network (ZFIN) is the database of genetic and genomic data for the zebrafish (Danio rerio) as a model organism.ZFIN provides a wide array of expertly curated, organized and cross-referenced zebrafish research data.ZFIN GENE: HMGA1A
hmga1a. Predicted to have transcription coregulator activity. Involved in regulation of RNA splicing. Predicted to localize to nucleus. Is expressed in DEL; central nervous system; heart; margin; and otic vesicle. Human ortholog (s) of this gene implicated in type 2 diabetes mellitus. Orthologous to human HMGA1 (high mobility group AT-hook 1).ZFIN GENE: VSX2
Involved in negative regulation of transcription by RNA polymerase II and regulation of neural retina development. Predicted to localize to nucleus. Is expressed in nervous system; neural tube; optic cup; and optic vesicle. Human ortholog (s) of this gene implicated in blindness and isolated microphthalmia 2.ZFIN GENE: TGFB1A
tgfb1a ID ZDB-GENE-030618-1 Name transforming growth factor, beta 1a Symbol tgfb1a Nomenclature History Previous Names. tgfb1 (); ai39657; wu:fb13a07 (); xx:ai39657; Type protein_coding_geneZFIN GENE: HE1.1
Description. Predicted to have metalloendopeptidase activity. Predicted to be involved in proteolysis. Is expressed in hatching gland and polster. Orthologous to human ASTL (astacin like metalloendopeptidase). Genome Resources. Alliance ( 1) Gene:569018 ( 1) VEGA:OTTDARG00000009891 ( 1)ZFIN GENE: SETA
seta ID ZDB-GENE-030131-2221 Name SET nuclear proto-oncogene a Symbol seta Nomenclature History Previous Names. ik:tdsubc_2c6; wu:fb99h10; xx:tdsubc_2c6; Type protein_coding_geneZFIN GENE: KCNA5
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate.ZFIN GENE: ANXA4
anxa4 ID ZDB-GENE-030707-1 Name annexin A4 Symbol anxa4 Nomenclature History Previous Names None Type protein_coding_gene Location Chr: 10 Mapping Details/Browsers DescriptionZFIN GENE: SPG11
spg11 ID ZDB-GENE-101017-1 Name SPG11 vesicle trafficking associated, spatacsin Symbol spg11 Nomenclature History Previous Names. spatacsin (); Type protein_coding_gene LocationZFIN GENE: OC90
oc90 ID ZDB-GENE-070912-300 Name otoconin 90 Symbol oc90 Nomenclature History Previous Names. otoc1 (); si:ch211-66b9.2; wu:fb30b04; Type protein_coding_gene Location Chr: 2 Mapping Details/BrowsersDescription
ZFIN GENE: RTN2B
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate.ZFIN GENE: ACTB2
actb2. Predicted to be a structural constituent of postsynaptic actin cytoskeleton. Predicted to localize to several cellular components, including actin filament; dense body; and focal adhesion. Is expressed in several structures, including blastomere; brain; eye; musculature system; and pleuroperitoneal region. ZFIN: ZEBRAFISH BOOK: CONTENTS This book is dedicated to the late George Streisinger. who first began the study of zebrafish in Eugene and. whose insight and gentle support got us all started. Adapted for the Web by ZFIN. Please address corrections and additions to: Monte Westerfield, Institute ofZFIN GENE: SPG11
spg11 ID ZDB-GENE-101017-1 Name SPG11 vesicle trafficking associated, spatacsin Symbol spg11 Nomenclature History Previous Names. spatacsin (); Type protein_coding_gene Location ZFIN PUBLICATION: JIA ET AL., 2020 A previous study suggested that human Coffin-Siris syndrome is related to the mutation of SOX11.Since the homozygous SOX11 mutant mice died soon after birth, no suitable model was available for the study of the pathogenic mechanism of Coffin-Siris syndrome. To solve this problem, we generated two viable homozygous zebrafish mutants, sox11a m/m andsox11b m/m.
ZFIN PUBLICATION: CHEN ET AL., 2020 Date: 2020: Source: Journal of Agricultural and Food Chemistry 68(40): 11170-11181 (Journal) Generate reference Registered Authors: ZFIN PUBLICATION: HAMON ET AL., 2020 In addition to the already described ligand L4a, two pyclen based lanthanide chelators, L4b and L4c, bearing two specific picolinate two-photon antennas (tailor-made for each targeted metal) and one acetate arm arranged in a dissymmetrical manner, have been synthesized, to form a complete family of lanthanide luminescent bioprobes: , , , and . ZFIN LAB: SKROMNE LAB Hayward, A.G., Joshi, P., Skromne, I. (2015) Spatiotemporal analysis of zebrafish hox gene regulation by Cdx4. Developmental dynamics : an official publication of ZFIN PUBLICATION: BOYLE ET AL., 2020 Plastic polymers such as polyvinyl chloride (PVC) may contain chemical additives, such as lead (Pb), that are leachable in aqueous solution. The fragmentation into microplastics (MPs) of plastics such as PVC may facilitate desorption of chemical additives and increase exposure ofaquatic animals.
ZFIN GENE: ZMP:0000001237 We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate.__
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ZFIN is hiring both a software developer and a curator! The Zebrafish Information Network (ZFIN) is the database of genetic and genomic data for the zebrafish (_Danio rerio_) as a model organism. ZFIN provides a wide array of expertly curated, organized and cross-referenced zebrafish research data.Learn More
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FIG. 1 OF ZHOU _ET AL._, 2020 Figure 1. Excessive exercise leads to pathological cardiac hypertrophy. (A,B) Representative images from control (A) and excessively-exercised zebrafish hearts (B) after 4 weeks of static and excessive-exercise treatment. (C–F) Representative pictures of Masson’s trichrome-stained ventricular tissues of control (C,D) and excessively-exercised zebrafish hearts (E,F). Blue area indicates fibrosis. (D,F) Are enlargements of the rectangle area in (C,E), respectively. Scale bar = 100 μm. (G) Quantitative analysis of Masson staining positive myocardial fibrosis area using Image J. ∗p < 0.05 by unpaired Student’s t-test. n = 3. (H,I) TEM images of control (H) and excessively exercised heart tissue (I). Scale bar = 5 μm. (J) RT-qPCR analysis of the expression of mitochondrial functional markers. ∗p < 0.05, ∗∗p < 0.01 by unpaired Student’s t-test. (K) Echocardiographic analysis of zebrafish after excessive exercise and in the static control (LVPW, LV posterior wall thickness). Error bars indicate SEM. n = 4. (L) The maximal oxygen consumption (MO2) of zebrafish after excessive exercise and in the control group was analyzed. n = 8. (M) RT-qPCR analysis of the expression of pathologic and physiological hypotrophy-related marker genes. ∗p < 0.05, ∗∗p < 0.01 by unpaired Student’s t-test. FIG. 8 OF CHEN _ET AL._, 2020 Chk1 and Wee1 are substrates of the Def-Capn3b complex. a Diagram showing the Capn3 recognition motif (left panel) and the locations of this motif in zebrafish, human and mouse Chk1 (middle panel) and Wee1 (right panel) proteins. b, c Western blot of Def, HA-p53R143H, HA-Chk1 and HA-Wee1S44A in WT embryos injected with HA-p53R143H, HA-Chk1 or HA-Wee1S44A mRNA combined with or without def mRNA (b). Western blot of HA-Chk1 and HA-Chk1Δ in WT embryos injected with HA-Chek1or HA-Chek1Δ mRNA combined with or without def mRNA (c). Total proteins were extracted at 8-h post-injection. β-Actin: loading control. d Western blot of the endogenous Chk1 and Wee1 proteins in WT and def−/− mutant embryos at 5dpf. Tubulin: loading control. e, f Co-immunostaining of Chk1 (e) or Wee1 (F) with Fibrillarin in the liver of WT with def−/− at 5dpf. DAPI: stain nuclei FIG. 5 OF IZARZUGAZA _ET AL._, 2020 Functional validation of candidate genes in zebrafish. a Phenotype of controls and morphants. Single genes and combinations of genes targeted by splicing morpholinos are indicated on the left. Upper panels: gross appearance of 48 hpf zebrafish embryos. Lower panel: examples of cardiac phenotypes of 48 hpf wt, control, and morphant embryos. Hearts were visualized by ISH with a probe against the cardiac marker myl7. b Quantification of phenotypes in wt, controls, morphants, and mRNA rescued morphants. Note the combinatorial effects on cardiac phenotypes when more than one gene is affected. c, d Expression of mef2cb in 10 somite stage zebrafish embryos, analyzed by qRT-PCR (c) and ISH (d). ISH staining intensity was quantified and analyzed using Student’s T test. *p < 0.05, **p < 0.01 FIGURE 6 OF MIN _ET AL._, 2020 Activation of Hif1a signaling impairs biliary morphogenesis. (A) Epifluorescence images showing PED6 accumulation in the gallbladder (arrows). (B) Confocal projection images showing BODIPY C5 staining (green) and Tp1:mCherry-CAAX expression (red; BEC membrane) in the liver (dotted lines). Scale bars: 200 (A), 50 μm (B). FIG. 1 OF CHEN _ET AL._, 2020 Generation of capn3b null mutant allele. a Generation of the capn3bΔ19Δ14 mutant allele. Upper panel: diagram showing the genomic structure of the zebrafish capn3b gene and the two mutated sites in capn3bΔ19Δ14 generated by TALEN and CRISPR-Cas9 approaches, respectively. Vertical bar: exon; line connecting vertical bar: intron. Lower panel: highlighting the 19 bp deletion (red letters) in 1st exon generated by the TALEN approach (on the left) and 14 bps deletion (red letters) in 3rd exon by the CRISPR-Cas9 approach (on the right), respectively. The position of nucleotide in the capn3b open reading frame (ORF) from the translation start codon ATG is provided. C120, Capn3b activity center. b Predicted peptide encoded by the capn3bΔ19 (∆19) and capn3bΔ19Δ14 (Δ19Δ14) mutant mRNA, respectively. The Δ19 mutation leads to an early stop codon in the capn3b ORF and resulted in a 30 amino acids (aa) long polypeptide, however, the Δ19 mutation potentially allows the ATG codon encoding M94 residue of WT Capn3b to be used as an alternative translation start codon to translate an N-terminus truncated peptide which harbors the activity center C120. The Δ19Δ14 mutation creates a new early stop codon in the presumed variant initiated from M94 after C120, therefore, capn3bΔ19Δ14 is likely a null allele. c Western blotting analysis of Capn3b in WT, capn3b capn3bΔ19Δ14, capn3bΔ19 at 5dpf. β-Actin: loading control. d Immunostaining of Capn3b in WT and capn3bΔ19Δ14 mutant embryos at 5dpf. Scale bar: 50 μm FIG. 4 OF MARSHALL-PHELPS _ET AL._, 2020 Neuronal activity drives peripheral nerve pathology in slc12a2b mutants. (A) Schematic overview of when, where and for how long TTX was applied to slc12a2bue58 mutants. (B) Confocal images of a Tg(mbp:EGFP-CAAX) control (top), slc12a2bue58 mutant (middle), and slc12a2bue58 mutant injected with TTX (bottom). Scale bar, 20 µm. (C) Quantitation of mean myelinated nerve diameter in controls, slc12a2bue58 mutants and slc12a2bue58 mutants injected with TTX (control 7.1 ± 0.5 µm vs. slc12a2bue58 13.4 ± 1.9 µm vs. slc12a2bue58+ TTX 6.1 ± 0.9 µm). Error bars represent mean ± SD. One-way ANOVA followed by Tukey’s multiple comparison test was used to assess statistical significance (ANOVA F(2,27) = 97, P < 0.0001). Each point represents an individual animal. ****, P < 0.0001. (D) Schematic overview of when, where, and for how long TTX was applied to either constitutive or glial-specific slc12a2b mutants. (E) Confocal images of 6 dpf slc12a2bue58 mutant larvae. Top and bottom panels show the same region of the pLLn before and 4–6 h after injection with either a control solution (left), or TTX (right). Scale bar, 20 µm. (F) Confocal images of 6 dpf Tg(mbp:EGFP-CAAX) larvae, in which slc12a2b has been targeted in myelinating glial cells. Top and bottom panels show the same region of the pLLn before and 4–6 h after injection with either a control solution (left) or TTX (right). Scale bar, 20 µm. (G) Quantitation of the change in mean myelinated nerve diameter following injection with either a control solution or TTX in slc12a2b mutants (G; sham injected −0.6 ± 1.1 µm vs. TTX −3.2 ± 1.6 µm, P < 0.0001) or animals in which slc12a2b has been disrupted specifically in myelinating glial cells (H; sham injected −0.4 ± 1 µm vs. TTX −2.5 ± 1.7 µm, P < 0.0001). Two-tailed Student’s t test was used to assess statistical significance. Each point represents an individual animal. ****, P < 0.0001. FIG. 4 OF ZHU _ET AL._, 2020 Disruption of arl13b reduces both precursor and differentiated cerebellar Purkinje cells. A–I Representative images of in situ hybridization using anti-sense probes against ptf1a and roraa to label Purkinje precursors and differentiated cells, respectively. The dorsomedial clusters of precursor of Purkinje cells and differentiated Purkinje neurons are selectively reduced (arrows). J–L’’ Immunostaining of mature Purkinje neurons using anti-parvalbumin antibody reveals that the dorsomedial Purkinje neurons are reduced in arl13b-deficient embryos. A–I, Scale bar 100 μm. J–L’’ Scalebar 50 μm.
FIGURE 7 OF MIN _ET AL._, 2020 Activation of Hif1a signaling increases BEC number and impairs the proper formation of bile canaliculi. (A) Confocal projection images showing intrahepatic biliary network by Tp1:GFP expression in the liver (dotted lines) at 5 dpf. Boxed regions are enlarged. (B) Confocal projection images showing Tp1:H2B-mCherry expression (red; BEC nuclei) in the liver (dotted lines) at 5 dpf. Quantification of BEC number is shown. (C) Confocal projection images showing Abcb11 and Tp1:GFP expression in the liver (dotted lines) at 5 dpf. Arrowheads point to bile canaliculi. n indicates the number of larvae used for quantification. Scale bars: 50 μm; error bars: ± SD. FIG. 9 OF CHEN _ET AL._, 2020 Hepatic accumulation of Chk1 and Wee1 in capn3bΔ19Δ14 at 3dpH. a Elevation of p53 protein level in capn3bΔ19Δ14 after PH. Western blot analysis of p53 at different time point after PH as indicated. Total proteins were extracted from the liver tissues of sham, 3dpH, 5dpH, 7dpH, 10dpH and 14dpH, respectively. GAPDH, loading control. b Western blot of Capn3b and Chk1 in WT and capn3bΔ19Δ14 mutant embryos at 3.5dpf and 5dpf grown at 30 °C and 34.5 °C, respectively. The embryos were shifted to 34.5 °C at 12hpf till the time of harvesting. β-Actin: loading control. c Western blot of Chk1 in WT and capn3bΔ19Δ14 sham and PH groups at 3dpH group. Fibrillarin: loading control. d Co-immunostaining of Wee1 and Fibrillarin in the liver of WT and capn3bΔ19Δ14 mutant fish at 3dpH. DAPI: staining nuclei FIG. 6 OF ZHU _ET AL._, 2020 Treating the arl13b mutants with lithium mitigates the morphological defects in the cerebellum. A–D Representative images of embryos treated with 50 mmol/L LiCl at 30–37 hpf, fixed at 3 dpf, and immunostained with anti-tubulin antibody to reveal cerebellar morphology. Treatment of wild-type embryos with Li+ does not affect the cerebellar morphology (A, B) (arrows). The morphological defects of the cerebellum in arl13b mutants treated with Li+ are partially rescued (C, D) (arrow). A–D, Scale bar 100 μm. E Statistics revealing that the proportion of arl13b mutant embryos with cerebellar defects is dramatically decreased by LiCl treatment. F–I’’ Representative images of Tg(neurod1:EGFP) transgenic embryos used to label granule cells. Treating wild-type transgenic embryos with Li+ causes no defect (F–G’’) (dashed ovals). Treating arl13b morphant transgenic embryos restores the dorsomedial cluster of granule cells (H–I’’) (dashed ovals). F–I’’, Scale bar 100 μm. J The proportion of arl13b morphant embryos with cerebellar defects in the dorsomedial clusters is dramatically reduced by LiCltreatment.
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ZFIN is hiring both a software developer and a curator! The Zebrafish Information Network (ZFIN) is the database of genetic and genomic data for the zebrafish (_Danio rerio_) as a model organism. ZFIN provides a wide array of expertly curated, organized and cross-referenced zebrafish research data.Learn More
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FIG. 1 OF ZHOU _ET AL._, 2020 Figure 1. Excessive exercise leads to pathological cardiac hypertrophy. (A,B) Representative images from control (A) and excessively-exercised zebrafish hearts (B) after 4 weeks of static and excessive-exercise treatment. (C–F) Representative pictures of Masson’s trichrome-stained ventricular tissues of control (C,D) and excessively-exercised zebrafish hearts (E,F). Blue area indicates fibrosis. (D,F) Are enlargements of the rectangle area in (C,E), respectively. Scale bar = 100 μm. (G) Quantitative analysis of Masson staining positive myocardial fibrosis area using Image J. ∗p < 0.05 by unpaired Student’s t-test. n = 3. (H,I) TEM images of control (H) and excessively exercised heart tissue (I). Scale bar = 5 μm. (J) RT-qPCR analysis of the expression of mitochondrial functional markers. ∗p < 0.05, ∗∗p < 0.01 by unpaired Student’s t-test. (K) Echocardiographic analysis of zebrafish after excessive exercise and in the static control (LVPW, LV posterior wall thickness). Error bars indicate SEM. n = 4. (L) The maximal oxygen consumption (MO2) of zebrafish after excessive exercise and in the control group was analyzed. n = 8. (M) RT-qPCR analysis of the expression of pathologic and physiological hypotrophy-related marker genes. ∗p < 0.05, ∗∗p < 0.01 by unpaired Student’s t-test. FIG. 8 OF CHEN _ET AL._, 2020 Chk1 and Wee1 are substrates of the Def-Capn3b complex. a Diagram showing the Capn3 recognition motif (left panel) and the locations of this motif in zebrafish, human and mouse Chk1 (middle panel) and Wee1 (right panel) proteins. b, c Western blot of Def, HA-p53R143H, HA-Chk1 and HA-Wee1S44A in WT embryos injected with HA-p53R143H, HA-Chk1 or HA-Wee1S44A mRNA combined with or without def mRNA (b). Western blot of HA-Chk1 and HA-Chk1Δ in WT embryos injected with HA-Chek1or HA-Chek1Δ mRNA combined with or without def mRNA (c). Total proteins were extracted at 8-h post-injection. β-Actin: loading control. d Western blot of the endogenous Chk1 and Wee1 proteins in WT and def−/− mutant embryos at 5dpf. Tubulin: loading control. e, f Co-immunostaining of Chk1 (e) or Wee1 (F) with Fibrillarin in the liver of WT with def−/− at 5dpf. DAPI: stain nuclei FIG. 5 OF IZARZUGAZA _ET AL._, 2020 Functional validation of candidate genes in zebrafish. a Phenotype of controls and morphants. Single genes and combinations of genes targeted by splicing morpholinos are indicated on the left. Upper panels: gross appearance of 48 hpf zebrafish embryos. Lower panel: examples of cardiac phenotypes of 48 hpf wt, control, and morphant embryos. Hearts were visualized by ISH with a probe against the cardiac marker myl7. b Quantification of phenotypes in wt, controls, morphants, and mRNA rescued morphants. Note the combinatorial effects on cardiac phenotypes when more than one gene is affected. c, d Expression of mef2cb in 10 somite stage zebrafish embryos, analyzed by qRT-PCR (c) and ISH (d). ISH staining intensity was quantified and analyzed using Student’s T test. *p < 0.05, **p < 0.01 FIGURE 6 OF MIN _ET AL._, 2020 Activation of Hif1a signaling impairs biliary morphogenesis. (A) Epifluorescence images showing PED6 accumulation in the gallbladder (arrows). (B) Confocal projection images showing BODIPY C5 staining (green) and Tp1:mCherry-CAAX expression (red; BEC membrane) in the liver (dotted lines). Scale bars: 200 (A), 50 μm (B). FIG. 1 OF CHEN _ET AL._, 2020 Generation of capn3b null mutant allele. a Generation of the capn3bΔ19Δ14 mutant allele. Upper panel: diagram showing the genomic structure of the zebrafish capn3b gene and the two mutated sites in capn3bΔ19Δ14 generated by TALEN and CRISPR-Cas9 approaches, respectively. Vertical bar: exon; line connecting vertical bar: intron. Lower panel: highlighting the 19 bp deletion (red letters) in 1st exon generated by the TALEN approach (on the left) and 14 bps deletion (red letters) in 3rd exon by the CRISPR-Cas9 approach (on the right), respectively. The position of nucleotide in the capn3b open reading frame (ORF) from the translation start codon ATG is provided. C120, Capn3b activity center. b Predicted peptide encoded by the capn3bΔ19 (∆19) and capn3bΔ19Δ14 (Δ19Δ14) mutant mRNA, respectively. The Δ19 mutation leads to an early stop codon in the capn3b ORF and resulted in a 30 amino acids (aa) long polypeptide, however, the Δ19 mutation potentially allows the ATG codon encoding M94 residue of WT Capn3b to be used as an alternative translation start codon to translate an N-terminus truncated peptide which harbors the activity center C120. The Δ19Δ14 mutation creates a new early stop codon in the presumed variant initiated from M94 after C120, therefore, capn3bΔ19Δ14 is likely a null allele. c Western blotting analysis of Capn3b in WT, capn3b capn3bΔ19Δ14, capn3bΔ19 at 5dpf. β-Actin: loading control. d Immunostaining of Capn3b in WT and capn3bΔ19Δ14 mutant embryos at 5dpf. Scale bar: 50 μm FIG. 4 OF MARSHALL-PHELPS _ET AL._, 2020 Neuronal activity drives peripheral nerve pathology in slc12a2b mutants. (A) Schematic overview of when, where and for how long TTX was applied to slc12a2bue58 mutants. (B) Confocal images of a Tg(mbp:EGFP-CAAX) control (top), slc12a2bue58 mutant (middle), and slc12a2bue58 mutant injected with TTX (bottom). Scale bar, 20 µm. (C) Quantitation of mean myelinated nerve diameter in controls, slc12a2bue58 mutants and slc12a2bue58 mutants injected with TTX (control 7.1 ± 0.5 µm vs. slc12a2bue58 13.4 ± 1.9 µm vs. slc12a2bue58+ TTX 6.1 ± 0.9 µm). Error bars represent mean ± SD. One-way ANOVA followed by Tukey’s multiple comparison test was used to assess statistical significance (ANOVA F(2,27) = 97, P < 0.0001). Each point represents an individual animal. ****, P < 0.0001. (D) Schematic overview of when, where, and for how long TTX was applied to either constitutive or glial-specific slc12a2b mutants. (E) Confocal images of 6 dpf slc12a2bue58 mutant larvae. Top and bottom panels show the same region of the pLLn before and 4–6 h after injection with either a control solution (left), or TTX (right). Scale bar, 20 µm. (F) Confocal images of 6 dpf Tg(mbp:EGFP-CAAX) larvae, in which slc12a2b has been targeted in myelinating glial cells. Top and bottom panels show the same region of the pLLn before and 4–6 h after injection with either a control solution (left) or TTX (right). Scale bar, 20 µm. (G) Quantitation of the change in mean myelinated nerve diameter following injection with either a control solution or TTX in slc12a2b mutants (G; sham injected −0.6 ± 1.1 µm vs. TTX −3.2 ± 1.6 µm, P < 0.0001) or animals in which slc12a2b has been disrupted specifically in myelinating glial cells (H; sham injected −0.4 ± 1 µm vs. TTX −2.5 ± 1.7 µm, P < 0.0001). Two-tailed Student’s t test was used to assess statistical significance. Each point represents an individual animal. ****, P < 0.0001. FIG. 4 OF ZHU _ET AL._, 2020 Disruption of arl13b reduces both precursor and differentiated cerebellar Purkinje cells. A–I Representative images of in situ hybridization using anti-sense probes against ptf1a and roraa to label Purkinje precursors and differentiated cells, respectively. The dorsomedial clusters of precursor of Purkinje cells and differentiated Purkinje neurons are selectively reduced (arrows). J–L’’ Immunostaining of mature Purkinje neurons using anti-parvalbumin antibody reveals that the dorsomedial Purkinje neurons are reduced in arl13b-deficient embryos. A–I, Scale bar 100 μm. J–L’’ Scalebar 50 μm.
FIGURE 7 OF MIN _ET AL._, 2020 Activation of Hif1a signaling increases BEC number and impairs the proper formation of bile canaliculi. (A) Confocal projection images showing intrahepatic biliary network by Tp1:GFP expression in the liver (dotted lines) at 5 dpf. Boxed regions are enlarged. (B) Confocal projection images showing Tp1:H2B-mCherry expression (red; BEC nuclei) in the liver (dotted lines) at 5 dpf. Quantification of BEC number is shown. (C) Confocal projection images showing Abcb11 and Tp1:GFP expression in the liver (dotted lines) at 5 dpf. Arrowheads point to bile canaliculi. n indicates the number of larvae used for quantification. Scale bars: 50 μm; error bars: ± SD. FIG. 9 OF CHEN _ET AL._, 2020 Hepatic accumulation of Chk1 and Wee1 in capn3bΔ19Δ14 at 3dpH. a Elevation of p53 protein level in capn3bΔ19Δ14 after PH. Western blot analysis of p53 at different time point after PH as indicated. Total proteins were extracted from the liver tissues of sham, 3dpH, 5dpH, 7dpH, 10dpH and 14dpH, respectively. GAPDH, loading control. b Western blot of Capn3b and Chk1 in WT and capn3bΔ19Δ14 mutant embryos at 3.5dpf and 5dpf grown at 30 °C and 34.5 °C, respectively. The embryos were shifted to 34.5 °C at 12hpf till the time of harvesting. β-Actin: loading control. c Western blot of Chk1 in WT and capn3bΔ19Δ14 sham and PH groups at 3dpH group. Fibrillarin: loading control. d Co-immunostaining of Wee1 and Fibrillarin in the liver of WT and capn3bΔ19Δ14 mutant fish at 3dpH. DAPI: staining nuclei FIG. 6 OF ZHU _ET AL._, 2020 Treating the arl13b mutants with lithium mitigates the morphological defects in the cerebellum. A–D Representative images of embryos treated with 50 mmol/L LiCl at 30–37 hpf, fixed at 3 dpf, and immunostained with anti-tubulin antibody to reveal cerebellar morphology. Treatment of wild-type embryos with Li+ does not affect the cerebellar morphology (A, B) (arrows). The morphological defects of the cerebellum in arl13b mutants treated with Li+ are partially rescued (C, D) (arrow). A–D, Scale bar 100 μm. E Statistics revealing that the proportion of arl13b mutant embryos with cerebellar defects is dramatically decreased by LiCl treatment. F–I’’ Representative images of Tg(neurod1:EGFP) transgenic embryos used to label granule cells. Treating wild-type transgenic embryos with Li+ causes no defect (F–G’’) (dashed ovals). Treating arl13b morphant transgenic embryos restores the dorsomedial cluster of granule cells (H–I’’) (dashed ovals). F–I’’, Scale bar 100 μm. J The proportion of arl13b morphant embryos with cerebellar defects in the dorsomedial clusters is dramatically reduced by LiCltreatment.
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ZFIN logo designed by Kari Pape Home page banner reprinted from Hearing Research, 341, Monroe, J.D. et al., Hearing sensitivity differs between zebrafish lines used in auditory research, 220-231, Copyright (2016) with permission from Elsevier Your Input WelcomeYour Input Welcome
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