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PROTEIN A ELISA KIT
The Protein A EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours.Absorbance is read at 450 nm. Ultra-sensitive quantification ( 1 ppm ) of Protein A residuals in purified humanized monoclonal antibody preparations.This kit recognizes four different Protein A ABSORBANCE 96 PLATE READER The Absorbance 96 Plate Reader is designed to run everyday assays with ease and speed. The four standard filters – 405, 450, 492, 620 nm – allow for a wide variety of ELISAs such as PNPP, ABTS, OPD and TMB. Other filter combinations for protein (Bradford or BCA), cell-based (MTT, XTT), or cell density assays can also be provided. MMP-2 (CATALYTIC DOMAIN) (HUMAN), (RECOMBINANT) Active recombinant matrix metalloproteinase-2 (MMP-2, gelatinase A, 72kDa type IV collagenase) cloned from human cDNA. The enzyme consists of residues Tyr 110 -Asp 452, which comprises the catalytic/fibronectin domain of human MMP-2, with a C-terminal purification tag. Liquid. In 50mM TRIS-HCl, pH 7.5, containing 300mM NaCl, 5mM CaCl 2, 20µM NF-ΚB SN50 CELL PERMEABLE INHIBITORY PEPTIDE Sequence contains the nuclear localization sequence (NLS residues 360-369) of the transcription factor NF-κB p50 linked to a peptide cell-permeablization sequence, the hydrophobic region (h-region) of the signal peptide of Kaposi fibroblast growth factor (K-FGF). Peptide inhibits translocation of the NF-κB active complex into the nucleus. AUTO-UBIQUITINYLATION KIT Auto-ubiquitinylation kit. BML-UW0970-0001. 10 tests. 269.00 USD. Do you need bulk/larger quantities? Qualitative assessment of an Ub E3 ligase enzyme’s activity through its ability to auto-ubiquitinylate. Testing of proteins for auto-ubiquitinylation activity allowing theiridentification as
PROTEOSTAT® PROTEIN AGGREGATION ASSAY PROTEOSTAT ® Protein aggregation assay provides a simple, homogenous assay format for monitoring peptide and protein aggregation in solution. This is useful for defining optimal storage formulations for proteins, for screening of compounds that promote or inhibit protein aggregation and potentially for the sensitive measurement of molecularchaperone activity.
WHAT ARE THE DIFFERENCES BETWEEN IHC AND ISH AND HOW DO Immunohistochemistry (IHC) is an invaluable tool for the detection, localization, and quantification of antigens in preserved tissue for research and diagnostic purposes. This method is widely used in diagnostics and research because of its convenience, reliability, and versatility. In IHC, antigen-antibody complexes can be visualized by fluorescence detection WHICH CONTROLS TO USE IN ELISA ASSAYS? It is therefore recommended to include B0 controls into every assay run of competitive ELISAs, even if the assay and sample/matrix combination is well established. Total Activity (TA) Controls serve to ensure enzymatic activity of the enzyme-conjugated antibody, biotin oranalyte.
HOME - ENZO LIFE SCIENCESPRODUCTSSCIENCE CENTERSUPPORTCELL BIOLOGYGENOMICSIMMUNOASSAYS & BIOCHEMICAL ASSAYS Meet Enzo Life Sciences. Enzo Life Sciences supports and participates in a variety of international conferences and symposia. View our Conference Calendar. SCREEN-WELL COMPOUND LIBRARIES SCREEN-WELL® Compound Libraries complement our wide range of small molecules. Enzo Life Sciences has a long and successful track record in identifying, synthesizing and commercializing known bioactives for use as research tool compounds and in assemblingPROTEIN A ELISA KIT
The Protein A EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours.Absorbance is read at 450 nm. Ultra-sensitive quantification ( 1 ppm ) of Protein A residuals in purified humanized monoclonal antibody preparations.This kit recognizes four different Protein A ABSORBANCE 96 PLATE READER The Absorbance 96 Plate Reader is designed to run everyday assays with ease and speed. The four standard filters – 405, 450, 492, 620 nm – allow for a wide variety of ELISAs such as PNPP, ABTS, OPD and TMB. Other filter combinations for protein (Bradford or BCA), cell-based (MTT, XTT), or cell density assays can also be provided. MMP-2 (CATALYTIC DOMAIN) (HUMAN), (RECOMBINANT) Active recombinant matrix metalloproteinase-2 (MMP-2, gelatinase A, 72kDa type IV collagenase) cloned from human cDNA. The enzyme consists of residues Tyr 110 -Asp 452, which comprises the catalytic/fibronectin domain of human MMP-2, with a C-terminal purification tag. Liquid. In 50mM TRIS-HCl, pH 7.5, containing 300mM NaCl, 5mM CaCl 2, 20µM NF-ΚB SN50 CELL PERMEABLE INHIBITORY PEPTIDE Sequence contains the nuclear localization sequence (NLS residues 360-369) of the transcription factor NF-κB p50 linked to a peptide cell-permeablization sequence, the hydrophobic region (h-region) of the signal peptide of Kaposi fibroblast growth factor (K-FGF). Peptide inhibits translocation of the NF-κB active complex into the nucleus. AUTO-UBIQUITINYLATION KIT Auto-ubiquitinylation kit. BML-UW0970-0001. 10 tests. 269.00 USD. Do you need bulk/larger quantities? Qualitative assessment of an Ub E3 ligase enzyme’s activity through its ability to auto-ubiquitinylate. Testing of proteins for auto-ubiquitinylation activity allowing theiridentification as
PROTEOSTAT® PROTEIN AGGREGATION ASSAY PROTEOSTAT ® Protein aggregation assay provides a simple, homogenous assay format for monitoring peptide and protein aggregation in solution. This is useful for defining optimal storage formulations for proteins, for screening of compounds that promote or inhibit protein aggregation and potentially for the sensitive measurement of molecularchaperone activity.
WHAT ARE THE DIFFERENCES BETWEEN IHC AND ISH AND HOW DO Immunohistochemistry (IHC) is an invaluable tool for the detection, localization, and quantification of antigens in preserved tissue for research and diagnostic purposes. This method is widely used in diagnostics and research because of its convenience, reliability, and versatility. In IHC, antigen-antibody complexes can be visualized by fluorescence detection WHICH CONTROLS TO USE IN ELISA ASSAYS? It is therefore recommended to include B0 controls into every assay run of competitive ELISAs, even if the assay and sample/matrix combination is well established. Total Activity (TA) Controls serve to ensure enzymatic activity of the enzyme-conjugated antibody, biotin oranalyte.
CAMP COMPLETE ELISA KIT This is a colorimetric competitive immunoassay kit for the quantitative determination of extracellular cAMP diluted in buffer or intracellular cAMP in cells or tissues lysed in 0.1 M HCl. 0.1M HCl is used to stop endogenous phosphodiesterase activity and stabilize the released cyclic AMP.DOPAMINE ELISA KIT
High-throughput immunoassay measures up to 40 samples in duplicate per 96 well kit. Pre-coated plates reduce errors and save time. The Dopamine ELISA kit is a colorimetric immunoassay kit with results in < 2 hours. Absorbance is read at 450 nm. Measure up to 40 samples in duplicate per 96 well kit.FLUOFORTE® REAGENT
Fluorescent calcium indicator dye developed to improve cell loading and calcium response while maintaining the convenient Fluo-3, Fluo-4 and Calcium 4 spectral wavelength of maximum excitation at ~490 nm and maximum emission at ~520 nm. Relative to the cited dyes, FLUOFORTE ® AM dye yields the brightest signal and largest assay window.CORTISOL ELISA KIT
Easy-to-use - pre-coated plates and liquid color-coded reagents save time and reduce errors. The Cortisol EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 3 hours. Absorbance is read at 405 nm. This kit is highly cited in peer-reviewedpublications.
CYTO-ID® AUTOPHAGY DETECTION KIT 2.0 CYTO-ID ® Autophagy Detection Kit 2.0 measures autophagic vacuoles and monitors autophagic flux in lysosomally inhibited live cells using a novel dye that selectively labels accumulated autophagic vacuoles. The dye has been optimized through the identification of titratable functional moieties that allow for minimal staining of lysosomes while exhibiting bright fluorescence upon incorporation SUBSTANCE P ELISA KIT Substance P is an undecapeptide hormone (RPKPQQFFGLM) that mediates a number of biological activities including nausea, anxiety, pain, and vasodilation. It is a member of the tachykinin family of neuropeptides, and is widely distributed in the peripheral and WHICH CONTROLS TO USE IN ELISA ASSAYS? To understand the types of controls and which of them to apply, it is important to understand the different types of ELISAs first (see our Tech Note “What are the differences between ELISA assay types?” for a more in-depth review). Direct ELISAs have different needs for controls than sandwich or competitive ELISAs, and different means of sample preparation or detection require different NGS WORKFLOW AND FUNDAMENTALS OF SAMPLE PREPARATION NGS Workflow and Fundamentals of Sample Preparation. Next Generation Sequencing (NGS) is a powerful and accessible high-throughput tool to gain insight into potentially any species at the genomic, transcriptomic or epigenetic level. Since the introduction of the groundbreaking Sanger method in 1977, fast advancements in sequencingtechnologies
WHAT IS THE DIFFERENCE BETWEEN TH1- AND TH2- T CELLS Lymphocytes T (or T cells) are key mediators of adaptive immunity, the part of the immune system capable of generating a pathogen-specific, long-lasting defense mechanism E. COLI HOST CELL PROTEIN ELISA KIT ENZ-KIT127-0001. 96 wells. 699.00 USD. Do you need bulk/larger quantities? Sensitive measurement of E. coli host cell protein, detecting as little as 30 ng/ml. High throughput format with results in 3 hours. Fully quantitative results that surpass semi-quantitative Western blot analysis. The E. coli host cell protein ELISA kit is acomplete
HOME - ENZO LIFE SCIENCESPRODUCTSSCIENCE CENTERSUPPORTCELL BIOLOGYGENOMICSIMMUNOASSAYS & BIOCHEMICAL ASSAYS Meet Enzo Life Sciences. Enzo Life Sciences supports and participates in a variety of international conferences and symposia. View our Conference Calendar. ELISA KITS - IMMUNOASSAYS - ENZO LIFE SCIENCES Reliable detection of analytes that are present at low concentrations is a critical need for early diagnosis and treatment of various diseases. Enzo Life Sciences has developed a highly sensitive ELISA Amplification System to be used with your most difficult-to-detect markers. Increase sensitivity up to 50-fold over traditional ELISAkits.
SCREEN-WELL COMPOUND LIBRARIES SCREEN-WELL® Compound Libraries complement our wide range of small molecules. Enzo Life Sciences has a long and successful track record in identifying, synthesizing and commercializing known bioactives for use as research tool compounds and in assembling 12(S)-HETE ELISA KIT Highly sensitive assay, measure as little as 146 pg/ml. Reliable and reproducible results day-after-day and lot-after-lot. The 12 (S)-HETE EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 5 hours. Absorbance is read at 405 nm. Fast, accurate, and reliable results are obtained with ready-to-use liquid color-coded CAMP COMPLETE ELISA KIT This is a colorimetric competitive immunoassay kit for the quantitative determination of extracellular cAMP diluted in buffer or intracellular cAMP in cells or tissues lysed in 0.1 M HCl. 0.1M HCl is used to stop endogenous phosphodiesterase activity and stabilize the released cyclic AMP. The 0.1M HCl treated samples are then analyzeddirectly
SP-5,6-DICHLORO-CBIMPS BML-CN120-0001. 1 mg. 259.00 USD. Do you need bulk/larger quantities? Replaces Prod. #: ALX-480-047. A cAMP analog which is a potent and specific activator of cAMP-dependent protein kinase. It is cell permeable and is not significantly hydrolyzed by type I, II or IIIphosphodiesterases.
WHICH CONTROLS TO USE IN ELISA ASSAYS? To understand the types of controls and which of them to apply, it is important to understand the different types of ELISAs first (see our Tech Note “What are the differences between ELISA assay types?” for a more in-depth review). Direct ELISAs have different needs for controls than sandwich or competitive ELISAs, and different means of sample preparation or detection require different NEUTROPHIL ELASTASE (HUMAN), (PURIFIED) Neutrophil elastase (human), (purified) BML-SE284-0100. 100 µg. 340.00 USD. Do you need bulk/larger quantities? Functions in inflammation. Can cleave a variety of proteins, including fibrous elastin, collagen, clotting factors, TNF-α , and α 1-antichymotrypsin.
MONO- AND POLYUBIQUITINYLATED CONJUGATES MONOCLONAL Immunocytochemistry (ICC) analysis of ubiquitinylated protein conjugates using fluorescein labeled mono- and polyubiquitinylated conjugates mAb (FK2), (Prod. No. BML-PW1210): HeLa cells, pre-treated with proteasome inhibitor MG-132 (Prod. No. BML-PI102, or ENZ-51035) at 5μM for 12 hours prior to analysis with antibody at 1:250dilution.
MEGACD40L® PROTEIN (SOLUBLE) (HUMAN), (RECOMBINANT) MEGACD40L. Protein (soluble) (human), (recombinant) High activity, high purity CD40L protein for co-stimulatory activation of an immune response. ALX-522-110-C010. 10 µg. 537.00 USD. HOME - ENZO LIFE SCIENCESPRODUCTSSCIENCE CENTERSUPPORTCELL BIOLOGYGENOMICSIMMUNOASSAYS & BIOCHEMICAL ASSAYS Meet Enzo Life Sciences. Enzo Life Sciences supports and participates in a variety of international conferences and symposia. View our Conference Calendar. ELISA KITS - IMMUNOASSAYS - ENZO LIFE SCIENCES Reliable detection of analytes that are present at low concentrations is a critical need for early diagnosis and treatment of various diseases. Enzo Life Sciences has developed a highly sensitive ELISA Amplification System to be used with your most difficult-to-detect markers. Increase sensitivity up to 50-fold over traditional ELISAkits.
SCREEN-WELL COMPOUND LIBRARIES SCREEN-WELL® Compound Libraries complement our wide range of small molecules. Enzo Life Sciences has a long and successful track record in identifying, synthesizing and commercializing known bioactives for use as research tool compounds and in assembling 12(S)-HETE ELISA KIT Highly sensitive assay, measure as little as 146 pg/ml. Reliable and reproducible results day-after-day and lot-after-lot. The 12 (S)-HETE EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 5 hours. Absorbance is read at 405 nm. Fast, accurate, and reliable results are obtained with ready-to-use liquid color-coded CAMP COMPLETE ELISA KIT This is a colorimetric competitive immunoassay kit for the quantitative determination of extracellular cAMP diluted in buffer or intracellular cAMP in cells or tissues lysed in 0.1 M HCl. 0.1M HCl is used to stop endogenous phosphodiesterase activity and stabilize the released cyclic AMP. The 0.1M HCl treated samples are then analyzeddirectly
SP-5,6-DICHLORO-CBIMPS BML-CN120-0001. 1 mg. 259.00 USD. Do you need bulk/larger quantities? Replaces Prod. #: ALX-480-047. A cAMP analog which is a potent and specific activator of cAMP-dependent protein kinase. It is cell permeable and is not significantly hydrolyzed by type I, II or IIIphosphodiesterases.
WHICH CONTROLS TO USE IN ELISA ASSAYS? To understand the types of controls and which of them to apply, it is important to understand the different types of ELISAs first (see our Tech Note “What are the differences between ELISA assay types?” for a more in-depth review). Direct ELISAs have different needs for controls than sandwich or competitive ELISAs, and different means of sample preparation or detection require different NEUTROPHIL ELASTASE (HUMAN), (PURIFIED) Neutrophil elastase (human), (purified) BML-SE284-0100. 100 µg. 340.00 USD. Do you need bulk/larger quantities? Functions in inflammation. Can cleave a variety of proteins, including fibrous elastin, collagen, clotting factors, TNF-α , and α 1-antichymotrypsin.
MONO- AND POLYUBIQUITINYLATED CONJUGATES MONOCLONAL Immunocytochemistry (ICC) analysis of ubiquitinylated protein conjugates using fluorescein labeled mono- and polyubiquitinylated conjugates mAb (FK2), (Prod. No. BML-PW1210): HeLa cells, pre-treated with proteasome inhibitor MG-132 (Prod. No. BML-PI102, or ENZ-51035) at 5μM for 12 hours prior to analysis with antibody at 1:250dilution.
MEGACD40L® PROTEIN (SOLUBLE) (HUMAN), (RECOMBINANT) MEGACD40L. Protein (soluble) (human), (recombinant) High activity, high purity CD40L protein for co-stimulatory activation of an immune response. ALX-522-110-C010. 10 µg. 537.00 USD. AXXORA - ENZO LIFE SCIENCES Axxora, an Enzo Life Sciences Company, is an innovative e-marketplace within the life sciences industry, offering over 30,000 unique reagents from a select group of specialty manufacturers complementary to ELS. Axxora.com’s novel, online catalog makes it easy for scientists and purchasing organizations to order these uniquesolutions from one
ANTIBODY SEARCH ENGINE Enzo Life Sciences offers thousands of antibodies against peptides, whole or partial proteins, and modified peptides or proteins. Backed by thousands of peer-reviewed citations, our Worry-free Antibody Trial Program, and expert technical support, you can count on Enzo antibodies to deliver reliable, consistent, accurate data. CAMP COMPLETE ELISA KIT This is a colorimetric competitive immunoassay kit for the quantitative determination of extracellular cAMP diluted in buffer or intracellular cAMP in cells or tissues lysed in 0.1 M HCl. 0.1M HCl is used to stop endogenous phosphodiesterase activity and stabilize the released cyclic AMP. WORRY-FREE ANTIBODY TRIAL PROGRAM Worry-free Antibody Trial Program. Finding the right antibody can be difficult and costly. Enzo Life Sciences understands this and is happy to offer a no-risk antibody trial program. Evaluate our antibodies in new applications or species not listed on our data sheet. If the antibody does not give you usable results, we’ll simply issue you a PROTEOSTAT® PROTEIN AGGREGATION ASSAY PROTEOSTAT ® Protein aggregation assay provides a simple, homogenous assay format for monitoring peptide and protein aggregation in solution. This is useful for defining optimal storage formulations for proteins, for screening of compounds that promote or inhibit protein aggregation and potentially for the sensitive measurement of molecularchaperone activity.
PEGYLATED PROTEIN ELISA KIT High-throughput format, analyze up to 35 samples in duplicate results in just 2 hours. The PEGylated Protein ELISA kit is a competitive assay specific to the backbone of poly (ethylene glycol) (PEG) and has been validated for use with a wide variety of PEG molecules including linear and branched forms as well as free and conjugated forms. WHICH CONTROLS TO USE IN ELISA ASSAYS? To understand the types of controls and which of them to apply, it is important to understand the different types of ELISAs first (see our Tech Note “What are the differences between ELISA assay types?” for a more in-depth review). Direct ELISAs have different needs for controls than sandwich or competitive ELISAs, and different means of sample preparation or detection require different IRISIN (HUMAN), (RECOMBINANT) (ACTIVE) Liquid. In 1X PBS. Treatment of 3T3L1 with 2µg/ml Irisin induces UCP-1 expression. Stable for up to a week when stored at +4°C and at least 6 months when stored at -80°C. Avoid freeze/thaw cycles. Irisin is a hormone that is involved in converting white adipose tissue WHAT ARE THE DIFFERENCES BETWEEN PCR, RT-PCR, QPCR, AND RT The PCR mechanism is as simple as its purpose: 1) double-stranded DNA (dsDNA) is heat denatured, 2) primers align to the single DNA strands and 3) the primers are extended by DNA polymerase, resulting in two copies of the original DNA strand. The denaturation, annealing, and elongation process over a series of temperatures and times is known as LYSO-ID® RED DETECTION KIT (GFP-CERTIFIED®) LYSO-ID ® Red Lysosomal Detection Kit (GFP-CERTIFIED ®) is an ideal choice for fluorescence co-localization imaging with GFP-tagged proteins, providing spectrally pure red signal.By contrast, many red-emitting lysosomal probes photoconvert to green fluorescent species or display metachromatic artifacts wherein emission occurs both in the red and green regions of the spectrum, leading to HOME - ENZO LIFE SCIENCESPRODUCTSSCIENCE CENTERSUPPORTCELL BIOLOGYGENOMICSIMMUNOASSAYS & BIOCHEMICAL ASSAYS Meet Enzo Life Sciences. Enzo Life Sciences supports and participates in a variety of international conferences and symposia. View our Conference Calendar. ELISA KITS - IMMUNOASSAYS - ENZO LIFE SCIENCES Reliable detection of analytes that are present at low concentrations is a critical need for early diagnosis and treatment of various diseases. Enzo Life Sciences has developed a highly sensitive ELISA Amplification System to be used with your most difficult-to-detect markers. Increase sensitivity up to 50-fold over traditional ELISAkits.
SCREEN-WELL COMPOUND LIBRARIES SCREEN-WELL® Compound Libraries complement our wide range of small molecules. Enzo Life Sciences has a long and successful track record in identifying, synthesizing and commercializing known bioactives for use as research tool compounds and in assembling 12(S)-HETE ELISA KIT Highly sensitive assay, measure as little as 146 pg/ml. Reliable and reproducible results day-after-day and lot-after-lot. The 12 (S)-HETE EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 5 hours. Absorbance is read at 405 nm. Fast, accurate, and reliable results are obtained with ready-to-use liquid color-coded CAMP COMPLETE ELISA KIT This is a colorimetric competitive immunoassay kit for the quantitative determination of extracellular cAMP diluted in buffer or intracellular cAMP in cells or tissues lysed in 0.1 M HCl. 0.1M HCl is used to stop endogenous phosphodiesterase activity and stabilize the released cyclic AMP. The 0.1M HCl treated samples are then analyzeddirectly
SP-5,6-DICHLORO-CBIMPS BML-CN120-0001. 1 mg. 259.00 USD. Do you need bulk/larger quantities? Replaces Prod. #: ALX-480-047. A cAMP analog which is a potent and specific activator of cAMP-dependent protein kinase. It is cell permeable and is not significantly hydrolyzed by type I, II or IIIphosphodiesterases.
WHICH CONTROLS TO USE IN ELISA ASSAYS? To understand the types of controls and which of them to apply, it is important to understand the different types of ELISAs first (see our Tech Note “What are the differences between ELISA assay types?” for a more in-depth review). Direct ELISAs have different needs for controls than sandwich or competitive ELISAs, and different means of sample preparation or detection require different NEUTROPHIL ELASTASE (HUMAN), (PURIFIED) Neutrophil elastase (human), (purified) BML-SE284-0100. 100 µg. 340.00 USD. Do you need bulk/larger quantities? Functions in inflammation. Can cleave a variety of proteins, including fibrous elastin, collagen, clotting factors, TNF-α , and α 1-antichymotrypsin.
MONO- AND POLYUBIQUITINYLATED CONJUGATES MONOCLONAL Immunocytochemistry (ICC) analysis of ubiquitinylated protein conjugates using fluorescein labeled mono- and polyubiquitinylated conjugates mAb (FK2), (Prod. No. BML-PW1210): HeLa cells, pre-treated with proteasome inhibitor MG-132 (Prod. No. BML-PI102, or ENZ-51035) at 5μM for 12 hours prior to analysis with antibody at 1:250dilution.
MEGACD40L® PROTEIN (SOLUBLE) (HUMAN), (RECOMBINANT) MEGACD40L. Protein (soluble) (human), (recombinant) High activity, high purity CD40L protein for co-stimulatory activation of an immune response. ALX-522-110-C010. 10 µg. 537.00 USD. HOME - ENZO LIFE SCIENCESPRODUCTSSCIENCE CENTERSUPPORTCELL BIOLOGYGENOMICSIMMUNOASSAYS & BIOCHEMICAL ASSAYS Meet Enzo Life Sciences. Enzo Life Sciences supports and participates in a variety of international conferences and symposia. View our Conference Calendar. ELISA KITS - IMMUNOASSAYS - ENZO LIFE SCIENCES Reliable detection of analytes that are present at low concentrations is a critical need for early diagnosis and treatment of various diseases. Enzo Life Sciences has developed a highly sensitive ELISA Amplification System to be used with your most difficult-to-detect markers. Increase sensitivity up to 50-fold over traditional ELISAkits.
SCREEN-WELL COMPOUND LIBRARIES SCREEN-WELL® Compound Libraries complement our wide range of small molecules. Enzo Life Sciences has a long and successful track record in identifying, synthesizing and commercializing known bioactives for use as research tool compounds and in assembling 12(S)-HETE ELISA KIT Highly sensitive assay, measure as little as 146 pg/ml. Reliable and reproducible results day-after-day and lot-after-lot. The 12 (S)-HETE EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 5 hours. Absorbance is read at 405 nm. Fast, accurate, and reliable results are obtained with ready-to-use liquid color-coded CAMP COMPLETE ELISA KIT This is a colorimetric competitive immunoassay kit for the quantitative determination of extracellular cAMP diluted in buffer or intracellular cAMP in cells or tissues lysed in 0.1 M HCl. 0.1M HCl is used to stop endogenous phosphodiesterase activity and stabilize the released cyclic AMP. The 0.1M HCl treated samples are then analyzeddirectly
SP-5,6-DICHLORO-CBIMPS BML-CN120-0001. 1 mg. 259.00 USD. Do you need bulk/larger quantities? Replaces Prod. #: ALX-480-047. A cAMP analog which is a potent and specific activator of cAMP-dependent protein kinase. It is cell permeable and is not significantly hydrolyzed by type I, II or IIIphosphodiesterases.
WHICH CONTROLS TO USE IN ELISA ASSAYS? To understand the types of controls and which of them to apply, it is important to understand the different types of ELISAs first (see our Tech Note “What are the differences between ELISA assay types?” for a more in-depth review). Direct ELISAs have different needs for controls than sandwich or competitive ELISAs, and different means of sample preparation or detection require different NEUTROPHIL ELASTASE (HUMAN), (PURIFIED) Neutrophil elastase (human), (purified) BML-SE284-0100. 100 µg. 340.00 USD. Do you need bulk/larger quantities? Functions in inflammation. Can cleave a variety of proteins, including fibrous elastin, collagen, clotting factors, TNF-α , and α 1-antichymotrypsin.
MONO- AND POLYUBIQUITINYLATED CONJUGATES MONOCLONAL Immunocytochemistry (ICC) analysis of ubiquitinylated protein conjugates using fluorescein labeled mono- and polyubiquitinylated conjugates mAb (FK2), (Prod. No. BML-PW1210): HeLa cells, pre-treated with proteasome inhibitor MG-132 (Prod. No. BML-PI102, or ENZ-51035) at 5μM for 12 hours prior to analysis with antibody at 1:250dilution.
MEGACD40L® PROTEIN (SOLUBLE) (HUMAN), (RECOMBINANT) MEGACD40L. Protein (soluble) (human), (recombinant) High activity, high purity CD40L protein for co-stimulatory activation of an immune response. ALX-522-110-C010. 10 µg. 537.00 USD. AXXORA - ENZO LIFE SCIENCES Axxora, an Enzo Life Sciences Company, is an innovative e-marketplace within the life sciences industry, offering over 30,000 unique reagents from a select group of specialty manufacturers complementary to ELS. Axxora.com’s novel, online catalog makes it easy for scientists and purchasing organizations to order these uniquesolutions from one
ANTIBODY SEARCH ENGINE Enzo Life Sciences offers thousands of antibodies against peptides, whole or partial proteins, and modified peptides or proteins. Backed by thousands of peer-reviewed citations, our Worry-free Antibody Trial Program, and expert technical support, you can count on Enzo antibodies to deliver reliable, consistent, accurate data. CAMP COMPLETE ELISA KIT This is a colorimetric competitive immunoassay kit for the quantitative determination of extracellular cAMP diluted in buffer or intracellular cAMP in cells or tissues lysed in 0.1 M HCl. 0.1M HCl is used to stop endogenous phosphodiesterase activity and stabilize the released cyclic AMP. WORRY-FREE ANTIBODY TRIAL PROGRAM Worry-free Antibody Trial Program. Finding the right antibody can be difficult and costly. Enzo Life Sciences understands this and is happy to offer a no-risk antibody trial program. Evaluate our antibodies in new applications or species not listed on our data sheet. If the antibody does not give you usable results, we’ll simply issue you a PROTEOSTAT® PROTEIN AGGREGATION ASSAY PROTEOSTAT ® Protein aggregation assay provides a simple, homogenous assay format for monitoring peptide and protein aggregation in solution. This is useful for defining optimal storage formulations for proteins, for screening of compounds that promote or inhibit protein aggregation and potentially for the sensitive measurement of molecularchaperone activity.
PEGYLATED PROTEIN ELISA KIT High-throughput format, analyze up to 35 samples in duplicate results in just 2 hours. The PEGylated Protein ELISA kit is a competitive assay specific to the backbone of poly (ethylene glycol) (PEG) and has been validated for use with a wide variety of PEG molecules including linear and branched forms as well as free and conjugated forms. WHICH CONTROLS TO USE IN ELISA ASSAYS? To understand the types of controls and which of them to apply, it is important to understand the different types of ELISAs first (see our Tech Note “What are the differences between ELISA assay types?” for a more in-depth review). Direct ELISAs have different needs for controls than sandwich or competitive ELISAs, and different means of sample preparation or detection require different IRISIN (HUMAN), (RECOMBINANT) (ACTIVE) Liquid. In 1X PBS. Treatment of 3T3L1 with 2µg/ml Irisin induces UCP-1 expression. Stable for up to a week when stored at +4°C and at least 6 months when stored at -80°C. Avoid freeze/thaw cycles. Irisin is a hormone that is involved in converting white adipose tissue WHAT ARE THE DIFFERENCES BETWEEN PCR, RT-PCR, QPCR, AND RT The PCR mechanism is as simple as its purpose: 1) double-stranded DNA (dsDNA) is heat denatured, 2) primers align to the single DNA strands and 3) the primers are extended by DNA polymerase, resulting in two copies of the original DNA strand. The denaturation, annealing, and elongation process over a series of temperatures and times is known as LYSO-ID® RED DETECTION KIT (GFP-CERTIFIED®) LYSO-ID ® Red Lysosomal Detection Kit (GFP-CERTIFIED ®) is an ideal choice for fluorescence co-localization imaging with GFP-tagged proteins, providing spectrally pure red signal.By contrast, many red-emitting lysosomal probes photoconvert to green fluorescent species or display metachromatic artifacts wherein emission occurs both in the red and green regions of the spectrum, leading to Online Purchasing Account You are logged on as Guest. LoginRegister a New
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