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life science.
PERKIN ELMER LS55 SPECTROFLUOROMETER Article Title: A Solid-in-Oil Nanodispersion System for Transcutaneous Immunotherapy of Cow’s Milk Allergies Article Snippet: ..The skins were subsequently cut into 16 pieces, and the FITC-BLG was extracted in PBS (1 mL) by vortexing for 18 h.ADMAX SYSTEM
Article Title: MicroRNA-181a Suppresses Mouse Granulosa Cell Proliferation by Targeting Activin Receptor IIA Article Snippet: In some experiments, the cells were culture in the presence of recombinant human/mouse/rat activin A (Catalog Number: 338-AC, R & D Systems, Minneapolis, MN, USA). ..Generation of Recombinant Adenovirus Adenoviruses harboring a 456-bp DNA fragmentADMAX KIT D
Article Title: Chm-1 gene-modified bone marrow mesenchymal stem cells maintain the chondrogenic phenotype of tissue-engineered cartilage Article Snippet: The PCR products were subcloned into the pDC316 expression adenovirus vector (pDC316-Chm-1) after restriction enzyme digestion. ..According to the manufacturer’s instructions for the AdMax Kit D (Microbix Biosystems Inc.,Toronto, ON, Canada 13 CM IMMOBILINE DRYSTRIPS Article Title: Anamorsin, a Novel Caspase-3 Substrate in Neurodegeneration * Article Snippet: Protein concentrations of samples were measured by a 2D Quant kit (GE Healthcare) as recommended by the manufacturer. ..Prior to IEF, Immobiline DryStrips (24 cm, pI 4–7 linear; GE Healthcare) were rehydrated in a sample buffer containing 1.5 mg of cellular lysates. .. SUM159 BREAST CANCER CELL LINES Article Title: Synergy in ERK activation by cytokine receptors and tyrosine kinase growth factor receptors Article Snippet: HEK-293-Jak2 cells grows in Dulbecco's modified Eagle's medium containing 1.0g/liter glucose (Cellgro, Inc), supplement with 10% fetal bovine serum. ..The human breast cancer cell line, T47D, was purchased from ATCC (Manassas, VA) and cultured in RPMI 1640 medium (CellgroL WNT3A CELLS
Journal: Frontiers in Pediatrics Article Title: Canonical WNT/β-Catenin Signaling Plays a Subordinate Role in Rhabdomyosarcomas doi: 10.3389/fped.2018.00378 Figure Lengend Snippet: Effects of FH535, XAV939, ß-catenin siRNA, or WNT3A on migration of RMS cell lines. (A) RMS tumor cells were pre-treated with FH535, XAV939, or WNT3A for 24 h before making the scratch. LUCIFERASE REPORTER PLASMIDS Article Title: Identification of a small molecule that primes the type I interferon response to cytosolic DNA Article Snippet: ..Briefly, the ISRE-luciferase reporter gene was amplified by PCR from pISRE-luciferase plasmid (Stratagene, Ref 219089), and inserted in a vector with a G418-resistance gene. ..This new plasmid was transfected in HEK-293 cells from ATCC, amplified in culture medium HUMAN CD8 | ZSGB-BIO INC | BIOZ > ZSGB-BIO Inc > human cd8 human cd8. Similar Products MOUSE N9 MICROGLIAL CELL LINE Article Title: Sodium butyrate has context-dependent actions on dipeptidyl peptidase-4 and other metabolic parameters Article Snippet: .. Mouse mesangial cells (ATCC, Manassas, VA, USA) were maintained in Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12 (DMEM/F12) supplemented with 10% heat-inactivated fetal bovine serum (FBS), with other culture conditions being similar to HepG2 BIOZ | RATINGS FOR LIFE-SCIENCE RESEARCHFLOW CYTOMETERSNEWSISOTYPEEPIGENETICSLYSATESSIGN IN Search for Bioz rated products from peer reviewed research papers inlife science.
PERKIN ELMER LS55 SPECTROFLUOROMETER Article Title: A Solid-in-Oil Nanodispersion System for Transcutaneous Immunotherapy of Cow’s Milk Allergies Article Snippet: ..The skins were subsequently cut into 16 pieces, and the FITC-BLG was extracted in PBS (1 mL) by vortexing for 18 h.ADMAX SYSTEM
Article Title: MicroRNA-181a Suppresses Mouse Granulosa Cell Proliferation by Targeting Activin Receptor IIA Article Snippet: In some experiments, the cells were culture in the presence of recombinant human/mouse/rat activin A (Catalog Number: 338-AC, R & D Systems, Minneapolis, MN, USA). ..Generation of Recombinant Adenovirus Adenoviruses harboring a 456-bp DNA fragmentADMAX KIT D
Article Title: Chm-1 gene-modified bone marrow mesenchymal stem cells maintain the chondrogenic phenotype of tissue-engineered cartilage Article Snippet: The PCR products were subcloned into the pDC316 expression adenovirus vector (pDC316-Chm-1) after restriction enzyme digestion. ..According to the manufacturer’s instructions for the AdMax Kit D (Microbix Biosystems Inc.,Toronto, ON, Canada 13 CM IMMOBILINE DRYSTRIPS Article Title: Anamorsin, a Novel Caspase-3 Substrate in Neurodegeneration * Article Snippet: Protein concentrations of samples were measured by a 2D Quant kit (GE Healthcare) as recommended by the manufacturer. ..Prior to IEF, Immobiline DryStrips (24 cm, pI 4–7 linear; GE Healthcare) were rehydrated in a sample buffer containing 1.5 mg of cellular lysates. .. SUM159 BREAST CANCER CELL LINES Article Title: Synergy in ERK activation by cytokine receptors and tyrosine kinase growth factor receptors Article Snippet: HEK-293-Jak2 cells grows in Dulbecco's modified Eagle's medium containing 1.0g/liter glucose (Cellgro, Inc), supplement with 10% fetal bovine serum. ..The human breast cancer cell line, T47D, was purchased from ATCC (Manassas, VA) and cultured in RPMI 1640 medium (CellgroL WNT3A CELLS
Journal: Frontiers in Pediatrics Article Title: Canonical WNT/β-Catenin Signaling Plays a Subordinate Role in Rhabdomyosarcomas doi: 10.3389/fped.2018.00378 Figure Lengend Snippet: Effects of FH535, XAV939, ß-catenin siRNA, or WNT3A on migration of RMS cell lines. (A) RMS tumor cells were pre-treated with FH535, XAV939, or WNT3A for 24 h before making the scratch. LUCIFERASE REPORTER PLASMIDS Article Title: Identification of a small molecule that primes the type I interferon response to cytosolic DNA Article Snippet: ..Briefly, the ISRE-luciferase reporter gene was amplified by PCR from pISRE-luciferase plasmid (Stratagene, Ref 219089), and inserted in a vector with a G418-resistance gene. ..This new plasmid was transfected in HEK-293 cells from ATCC, amplified in culture medium HUMAN CD8 | ZSGB-BIO INC | BIOZ > ZSGB-BIO Inc > human cd8 human cd8. Similar Products MOUSE N9 MICROGLIAL CELL LINE Article Title: Sodium butyrate has context-dependent actions on dipeptidyl peptidase-4 and other metabolic parameters Article Snippet: .. Mouse mesangial cells (ATCC, Manassas, VA, USA) were maintained in Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12 (DMEM/F12) supplemented with 10% heat-inactivated fetal bovine serum (FBS), with other culture conditions being similar to HepG2ADMAX SYSTEM
Article Title: MicroRNA-181a Suppresses Mouse Granulosa Cell Proliferation by Targeting Activin Receptor IIA Article Snippet: In some experiments, the cells were culture in the presence of recombinant human/mouse/rat activin A (Catalog Number: 338-AC, R & D Systems, Minneapolis, MN, USA). ..Generation of Recombinant Adenovirus Adenoviruses harboring a 456-bp DNA fragment TRUCOUNT COUNTING BEADS Article Title: Exposure to pesticides in utero impacts the fetal immune system and response to vaccination in infancy Article Snippet: Dendritic and CD4 T absolute cell counts Dendritic cells and CD4 T cells were enumerated from 50 μL of whole cord blood stained with antibodies in BD TruCount™ tubes or with 20 μL of CountBright™ counting beads (ThermoFisher Scientific). .. BIO SWIVELOCK ANCHOR Journal: Arthroscopy Techniques Article Title: The Crossing Internal Suture Augmentation Technique to Protect the All-Inside Anterior Cruciate Ligament Reconstruction Graft doi: 10.1016/j.eats.2017.08.038 Figure Lengend Snippet: The ends of the FiberTape are fixed to the tibia 1 to 2 cm below the exit of the tibial socket (arrow) using a 5.5-mm Bio-SwiveLock anchor with the knee in full EN VISION SYSTEM HRP LABELED POLYMER ANTI RABBIT Price from $9.99 to $1999.99. envision system hrp labeled polymer anti rabbit - by Bioz Stars , 2021-05. 86 / 100 stars.RAWCELLPATH
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NCM460 NORMAL COLONIC EPITHELIAL CELL LINE Article Title: Synthetic Artificial Long Non-coding RNA Shows Higher Efficiency in Specific Malignant Phenotype Inhibition Compared to the CRISPR/Cas Systems Article Snippet: ..Cell Lines and Cell Culture The human bladder cancer cell lines (T24, 5637, and SW780) and the human normal bladder epithelial cell line (SV-HUC1) are all bought from the American Type Culture Collection (ATCC, ManassasPROTEOMICSSTUDIES
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Article Title: MicroRNA-181a Suppresses Mouse Granulosa Cell Proliferation by Targeting Activin Receptor IIA Article Snippet: In some experiments, the cells were culture in the presence of recombinant human/mouse/rat activin A (Catalog Number: 338-AC, R & D Systems, Minneapolis, MN, USA). ..Generation of Recombinant Adenovirus Adenoviruses harboring a 456-bp DNA fragment SUM159 BREAST CANCER CELL LINES Article Title: Synergy in ERK activation by cytokine receptors and tyrosine kinase growth factor receptors Article Snippet: HEK-293-Jak2 cells grows in Dulbecco's modified Eagle's medium containing 1.0g/liter glucose (Cellgro, Inc), supplement with 10% fetal bovine serum. ..The human breast cancer cell line, T47D, was purchased from ATCC (Manassas, VA) and cultured in RPMI 1640 medium (CellgroAPI ZYM KIT
Article Title: Rapid Methods for Testing the Efficacy of Sterilization-Grade Filter Membranes Article Snippet: Fluorescence from derived B. diminuta ::pBSLGFP1 recombinants was quantitated using a fluorospectrophotometer (model RF1501; Shimadzu) set to low gain. ..Biochemical tests on B. diminuta recombinant strains using API 20 NE and API ZYM test kits (Biomerieux) were carried out accordingCROSPOVIDONE
Figure Legend Snippet: Contour diagrams of the relationships among the formulation variable levels, croscarmellose sodium (CCS, Ac-Di-Sol ® ), crospovidone (Cros), and microcrystalline cellulose (MCC) in fixed levels of magnesium stearate (Mg St, 2.0%) and silicon dioxide (SiO 2 , 3.0%) at the level of 50% of Wuzi Yanzong dry powder extract; ( a ) effects on disintegration time; ( b ) effects MIRCURY RNA ISOLATION KIT BIOFLUIDS Figure Legend Snippet: miRNA recovery of different extraction kits were evaluated by the C q values for spike-in controls (cel-miR-39 and cel-miR-54) assayed in the (a) TaqMan and (b) miScript RT-qPCR system. Box plots with whiskers show 5–95 percentile of the C q values for spike-in controls and the corresponding CVs are shown. LUCIFERASE REPORTER PLASMIDS Article Title: Identification of a small molecule that primes the type I interferon response to cytosolic DNA Article Snippet: ..Briefly, the ISRE-luciferase reporter gene was amplified by PCR from pISRE-luciferase plasmid (Stratagene, Ref 219089), and inserted in a vector with a G418-resistance gene. ..This new plasmid was transfected in HEK-293 cells from ATCC, amplified in culture medium CIRCULATING TUMOR CELL KIT Journal: Radiation Oncology (London, England) Article Title: Detection and dynamics of circulating tumor cells in patients with high-risk prostate cancer treated with radiotherapy and hormones: a prospective phase II study doi: 10.1186/s13014-020-01577-5 Figure Lengend Snippet: CTCs detection rate at 4 timepoints: 1) baseline; 2) after neoadjuvant androgen deprivation therapy; 3) at the end of HUMAN CD8 | ZSGB-BIO INC | BIOZ > ZSGB-BIO Inc > human cd8 human cd8. Similar Products WALLAC ARVO MX 1420 MULTILABEL COUNTER SPECTROPHOTOMETER Subsequently, cells were pre-washed with PBS, and incubated with DCF-DA (1.0 µM) in darkness for 10 min. DCF-DA green fluorescence was assessed under a fluorescent microscope (Nikon Eclipe TE300, Nikon Instruments, Melville, NY) and quantitated by a Perkin-Elmer Victor V multilabel plate reader with excitation/emission filters set at 490and
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Article Title: MicroRNA-181a Suppresses Mouse Granulosa Cell Proliferation by Targeting Activin Receptor IIA Article Snippet: In some experiments, the cells were culture in the presence of recombinant human/mouse/rat activin A (Catalog Number: 338-AC, R & D Systems, Minneapolis, MN, USA). ..Generation of Recombinant Adenovirus Adenoviruses harboring a 456-bp DNA fragment SUM159 BREAST CANCER CELL LINES Article Title: Synergy in ERK activation by cytokine receptors and tyrosine kinase growth factor receptors Article Snippet: HEK-293-Jak2 cells grows in Dulbecco's modified Eagle's medium containing 1.0g/liter glucose (Cellgro, Inc), supplement with 10% fetal bovine serum. ..The human breast cancer cell line, T47D, was purchased from ATCC (Manassas, VA) and cultured in RPMI 1640 medium (CellgroAPI ZYM KIT
Article Title: Rapid Methods for Testing the Efficacy of Sterilization-Grade Filter Membranes Article Snippet: Fluorescence from derived B. diminuta ::pBSLGFP1 recombinants was quantitated using a fluorospectrophotometer (model RF1501; Shimadzu) set to low gain. ..Biochemical tests on B. diminuta recombinant strains using API 20 NE and API ZYM test kits (Biomerieux) were carried out accordingCROSPOVIDONE
Figure Legend Snippet: Contour diagrams of the relationships among the formulation variable levels, croscarmellose sodium (CCS, Ac-Di-Sol ® ), crospovidone (Cros), and microcrystalline cellulose (MCC) in fixed levels of magnesium stearate (Mg St, 2.0%) and silicon dioxide (SiO 2 , 3.0%) at the level of 50% of Wuzi Yanzong dry powder extract; ( a ) effects on disintegration time; ( b ) effects MIRCURY RNA ISOLATION KIT BIOFLUIDS Figure Legend Snippet: miRNA recovery of different extraction kits were evaluated by the C q values for spike-in controls (cel-miR-39 and cel-miR-54) assayed in the (a) TaqMan and (b) miScript RT-qPCR system. Box plots with whiskers show 5–95 percentile of the C q values for spike-in controls and the corresponding CVs are shown. LUCIFERASE REPORTER PLASMIDS Article Title: Identification of a small molecule that primes the type I interferon response to cytosolic DNA Article Snippet: ..Briefly, the ISRE-luciferase reporter gene was amplified by PCR from pISRE-luciferase plasmid (Stratagene, Ref 219089), and inserted in a vector with a G418-resistance gene. ..This new plasmid was transfected in HEK-293 cells from ATCC, amplified in culture medium CIRCULATING TUMOR CELL KIT Journal: Radiation Oncology (London, England) Article Title: Detection and dynamics of circulating tumor cells in patients with high-risk prostate cancer treated with radiotherapy and hormones: a prospective phase II study doi: 10.1186/s13014-020-01577-5 Figure Lengend Snippet: CTCs detection rate at 4 timepoints: 1) baseline; 2) after neoadjuvant androgen deprivation therapy; 3) at the end of HUMAN CD8 | ZSGB-BIO INC | BIOZ > ZSGB-BIO Inc > human cd8 human cd8. Similar Products WALLAC ARVO MX 1420 MULTILABEL COUNTER SPECTROPHOTOMETER Subsequently, cells were pre-washed with PBS, and incubated with DCF-DA (1.0 µM) in darkness for 10 min. DCF-DA green fluorescence was assessed under a fluorescent microscope (Nikon Eclipe TE300, Nikon Instruments, Melville, NY) and quantitated by a Perkin-Elmer Victor V multilabel plate reader with excitation/emission filters set at 490and
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Transformation of B. cinerea Protoplasts were generated using a mixture of Glucanex 200G (Novozymes, Denmark), lysing enzyme (Sigma Aldrich, St Louis, MO, USA), and Yatalase (Takara Bio Ins, Shiga, Japan) added to 15–20 µg of the linearized vector, and transformed according to Siewers et al. . .. WNT3A CONDITIONED MEDIUM Search for Bioz rated products from peer reviewed research papers inlife science.
SCRAMBLE RNA NEGATIVE CONTROLS Figure Legend Snippet: Kidins220 knockdown mimics the effort of miR-4638-5p overexpression on tumor cell growth ( A ) Western blot analysis of Kidins220 expression levels of PC3 and DU145 cells transduced with shRNA1-3 (S1-3). ( B ) The expression of RFP were observed under the fluorescence microscope 72 hours after PC3 and DU145 cells were infected with pCDH-sh Kidins220 (shKidins220) or the BD CELLQUEST PRO SOFTWARE Article Title: CDC7 kinase promotes MRE11 fork processing, modulating fork speed and chromosomal breakage Article Snippet: Cells were washed (PBS, 1% BSA, 5% Tween‐20) and DNA stained with DAPI (1 μg/ml, PBS, 1% BSA). ..Data were acquired on a BD FACSCanto II and analysed using FlowJo software. ..Cells were seeded at 3.5 × 105 cells/well in 60‐mm plates and incubated for 24 h. 16S RRNA GENE V3 V4 REGION Figure Legend Snippet: Operational taxonomic unit (OTU) analysis . All detected 16S rRNA V3-V4 regions from the original fecal sample and the cultured bacteria were summarized, yielding 445 OTUs in total. See the detected OTU number for each medium and sample in Table 2 and the detailed OTU information in Table S2. Techniques Used: Cell Culture. COMET SCORE SOFTWARE Article Title: Genotoxic Effects of Carotenoid Breakdown Products in Human Retinal Pigment Epithelial Cells Article Snippet: For the analysis of DNA in comet tails, the slides were examined at 250X magnification on Nixon fluorescent microscope (Nikon Eclipse 800 epifluorescence microscope). .. Fourty randomly selected non-overlapping cells were scored for DNA damage using the Comet ScoreHOME PAGE
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