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ABOUT US - ATUM
ATUM is based in the US, with a global customer base encompassing academia, government and the pharmaceutical, chemical, agricultural and biotechnology industries. ATUM is by far the most published synthetic biology vendor, providing expert support to and collaboration with scientists.PROTEINS - ATUM
We also offer Angiotensin Converting Enzyme (ACE-2) fused to human or murine Fc. ACE-2 is the host cell receptor responsible for mediating infection by SARS-CoV-2. For all other SARS-CoV-2/COVID-19 related proteins and stable pools, please inquire: email: info@atum.bio or call us 1-650-853-8347 x200.MAMMALIAN - ATUM
Mammalian cells are frequently used to produce secreted proteins. However, the effectiveness of a mammalian secretion signal sequence is highly dependent on the protein being secreted: signal sequences that work well for one chain of an antibody may be mediocre for another.PICHIA - ATUM
Cells are frozen on a dry ice/ethanol bath and stored at -80°C. Pichia Culture and Induction Protocol from ATUM. Notes. These strains have been jointly developed by BioGrammatics Inc. and ATUM. Proteins and peptides made with ATUM catalog Pichia strains (the “Pichia Strains”) can be used commercially without license obligations.GRNA DESIGN
The ATUM gRNA Design Tool enables: Suppression of off-target effects by avoiding sequences that match elsewhere in the genome. Direct ordering of your selected gRNAs in Electra CRISPR vectors, Transfection Ready. Enter your target gene name or your target sequence, and the design tool will identify all Cas9 target sites within the input sequence. CRISPR GRNA DESIGN TOOL CRISPR gRNA Design tool. Please wait, searching The ATUM gRNA Design Tool is provided "as is", with no explicit or implicit guarantees for any purpose. ATUM cannot guarantee the performance of any individual gRNA designed using the Tool. Custom CRISPR Constructs and CRISPR Catalog Vectors are not for use in humans.PROTEIN PAINT BOX
ProteinPaintbox® Synthetic non-Aequorea fluorescent and chromogenic proteins bring a world of color to your research.Overview. ATUM's synthetic fluorescent and chromogenic proteins (non-Aequorea) are an ideal source of protein coding sequences (genes) that can be easily excised using the flanking restriction sites and cloned into any other expression vector of choice. EXPRESSION SOLUTIONS expression Expression of active protein at high levels requires selecting the best coding sequence for a gene and the best vector fromwhich to express
CELL LINE DEVELOPMENT 2 2181 cell line development process Coding sequence optimization Expression of proteins in mammalian cells may be limited by codon usage and secretion signals. HOME - ATUMCELL LINE DEVELOPMENTPROTEIN PAINTBOXPROTEIN SERVICESABOUT USCAREERSGPS TECHNOLOGY A Fully Integrated Ecosystem. Every path to discovery is different. We focus on your goals and work hand in hand with you to achieve them. Whether you need expression optimization, recombinant protein production, antibody humanization and engineering, or cell line development, ATUM delivers solutions to advance your research and speed your path to market.ABOUT US - ATUM
ATUM is based in the US, with a global customer base encompassing academia, government and the pharmaceutical, chemical, agricultural and biotechnology industries. ATUM is by far the most published synthetic biology vendor, providing expert support to and collaboration with scientists.PROTEINS - ATUM
We also offer Angiotensin Converting Enzyme (ACE-2) fused to human or murine Fc. ACE-2 is the host cell receptor responsible for mediating infection by SARS-CoV-2. For all other SARS-CoV-2/COVID-19 related proteins and stable pools, please inquire: email: info@atum.bio or call us 1-650-853-8347 x200.MAMMALIAN - ATUM
Mammalian cells are frequently used to produce secreted proteins. However, the effectiveness of a mammalian secretion signal sequence is highly dependent on the protein being secreted: signal sequences that work well for one chain of an antibody may be mediocre for another.PICHIA - ATUM
Cells are frozen on a dry ice/ethanol bath and stored at -80°C. Pichia Culture and Induction Protocol from ATUM. Notes. These strains have been jointly developed by BioGrammatics Inc. and ATUM. Proteins and peptides made with ATUM catalog Pichia strains (the “Pichia Strains”) can be used commercially without license obligations.GRNA DESIGN
The ATUM gRNA Design Tool enables: Suppression of off-target effects by avoiding sequences that match elsewhere in the genome. Direct ordering of your selected gRNAs in Electra CRISPR vectors, Transfection Ready. Enter your target gene name or your target sequence, and the design tool will identify all Cas9 target sites within the input sequence. CRISPR GRNA DESIGN TOOL CRISPR gRNA Design tool. Please wait, searching The ATUM gRNA Design Tool is provided "as is", with no explicit or implicit guarantees for any purpose. ATUM cannot guarantee the performance of any individual gRNA designed using the Tool. Custom CRISPR Constructs and CRISPR Catalog Vectors are not for use in humans.PROTEIN PAINT BOX
ProteinPaintbox® Synthetic non-Aequorea fluorescent and chromogenic proteins bring a world of color to your research.Overview. ATUM's synthetic fluorescent and chromogenic proteins (non-Aequorea) are an ideal source of protein coding sequences (genes) that can be easily excised using the flanking restriction sites and cloned into any other expression vector of choice. EXPRESSION SOLUTIONS expression Expression of active protein at high levels requires selecting the best coding sequence for a gene and the best vector fromwhich to express
CELL LINE DEVELOPMENT 2 2181 cell line development process Coding sequence optimization Expression of proteins in mammalian cells may be limited by codon usage and secretion signals.ABOUT US - ATUM
ATUM is based in the US, with a global customer base encompassing academia, government and the pharmaceutical, chemical, agricultural and biotechnology industries. ATUM is by far the most published synthetic biology vendor, providing expert support to and collaboration with scientists.PROTEINS - ATUM
We also offer Angiotensin Converting Enzyme (ACE-2) fused to human or murine Fc. ACE-2 is the host cell receptor responsible for mediating infection by SARS-CoV-2. For all other SARS-CoV-2/COVID-19 related proteins and stable pools, please inquire: email: info@atum.bio or call us 1-650-853-8347 x200.ELECTRA SYSTEM
ATUM has developed a simple, one-tube, universal cloning process that can be performed in a 5 minute bench-top reaction with the fidelity of a restriction based cloning system. The Electra system uses the type IIS restriction enzyme SapI, which recognizes a 7bp non-palindromic recognition sequence and leaves a 3bp 5’ overhang after digestion. BACTERIAL EXPRESSION VECTORS ATUM can even test your gene for high expression. Expression Test is now available for bacterial vector panels with a volume discount. Cost for expression testing is $395.00 USD per gene per vector with an additional 10% off the total. Prices are in addition to regular genesynthesis fees.
CELL LINE DEVELOPMENT ATUM's Leap-In transposases and synthetic transposons enable a cost-effective, easily implemented cell line development workflow. The technology delivers high productivity pools and cell lines, resulting in consistent product quality. The Leap-In technology underpins ATUM's cell line development services and is also licensable for yourin-house
PICHIA - ATUM
Cells are frozen on a dry ice/ethanol bath and stored at -80°C. Pichia Culture and Induction Protocol from ATUM. Notes. These strains have been jointly developed by BioGrammatics Inc. and ATUM. Proteins and peptides made with ATUM catalog Pichia strains (the “Pichia Strains”) can be used commercially without license obligations.CRISPR - ATUM
Overview - CRISPR Vectors. ATUM’s CRISPR vectors comprise a gene encoding the Cas9 nuclease, and a cassette for expressing the guide RNA which targets the nuclease. Cas9 nucleases are directed by a 20nt guide sequence to cleave almost any genomic locus. The resulting chromosomal break will normally be repaired via non-homologous endjoining
MAMMALIAN EXPRESSION VECTORS ATUM′s pD600 series of mammalian transient expression vectors are still available. These vectors use the strong CMV/IE enhancer/promoter and an SV40 replication origin to give high levels of protein expression in mammalian cells. Although most pD600s have mammalian selectable markers, we recommend the Bifunctional Leap-In® vectorsfor
EXPRESSION SOLUTIONS expression Expression of active protein at high levels requires selecting the best coding sequence for a gene and the best vector fromwhich to express
YEAST EXPRESSION VECTORS Overview - Yeast Vectors. The methylotrophic yeast Pichia pastoris is a useful system for the expression of milligram-to-gram quantities of protein for both basic laboratory research and industrial manufacturing. ATUM offers Pichia vectors with a choice of promotersfor
HOME - ATUMCELL LINE DEVELOPMENTPROTEIN PAINTBOXPROTEIN SERVICESABOUT USCAREERSGPS TECHNOLOGY A Fully Integrated Ecosystem. Every path to discovery is different. We focus on your goals and work hand in hand with you to achieve them. Whether you need expression optimization, recombinant protein production, antibody humanization and engineering, or cell line development, ATUM delivers solutions to advance your research and speed your path to market.ABOUT US - ATUM
ATUM is based in the US, with a global customer base encompassing academia, government and the pharmaceutical, chemical, agricultural and biotechnology industries. ATUM is by far the most published synthetic biology vendor, providing expert support to and collaboration with scientists.PROTEINS - ATUM
We also offer Angiotensin Converting Enzyme (ACE-2) fused to human or murine Fc. ACE-2 is the host cell receptor responsible for mediating infection by SARS-CoV-2. For all other SARS-CoV-2/COVID-19 related proteins and stable pools, please inquire: email: info@atum.bio or call us 1-650-853-8347 x200.MAMMALIAN - ATUM
Mammalian cells are frequently used to produce secreted proteins. However, the effectiveness of a mammalian secretion signal sequence is highly dependent on the protein being secreted: signal sequences that work well for one chain of an antibody may be mediocre for another.PICHIA - ATUM
Cells are frozen on a dry ice/ethanol bath and stored at -80°C. Pichia Culture and Induction Protocol from ATUM. Notes. These strains have been jointly developed by BioGrammatics Inc. and ATUM. Proteins and peptides made with ATUM catalog Pichia strains (the “Pichia Strains”) can be used commercially without license obligations.GRNA DESIGN
The ATUM gRNA Design Tool enables: Suppression of off-target effects by avoiding sequences that match elsewhere in the genome. Direct ordering of your selected gRNAs in Electra CRISPR vectors, Transfection Ready. Enter your target gene name or your target sequence, and the design tool will identify all Cas9 target sites within the input sequence. CRISPR GRNA DESIGN TOOL CRISPR gRNA Design tool. Please wait, searching The ATUM gRNA Design Tool is provided "as is", with no explicit or implicit guarantees for any purpose. ATUM cannot guarantee the performance of any individual gRNA designed using the Tool. Custom CRISPR Constructs and CRISPR Catalog Vectors are not for use in humans.PROTEIN PAINT BOX
ProteinPaintbox® Synthetic non-Aequorea fluorescent and chromogenic proteins bring a world of color to your research.Overview. ATUM's synthetic fluorescent and chromogenic proteins (non-Aequorea) are an ideal source of protein coding sequences (genes) that can be easily excised using the flanking restriction sites and cloned into any other expression vector of choice. EXPRESSION SOLUTIONS expression Expression of active protein at high levels requires selecting the best coding sequence for a gene and the best vector fromwhich to express
CELL LINE DEVELOPMENT 2 2181 cell line development process Coding sequence optimization Expression of proteins in mammalian cells may be limited by codon usage and secretion signals. HOME - ATUMCELL LINE DEVELOPMENTPROTEIN PAINTBOXPROTEIN SERVICESABOUT USCAREERSGPS TECHNOLOGY A Fully Integrated Ecosystem. Every path to discovery is different. We focus on your goals and work hand in hand with you to achieve them. Whether you need expression optimization, recombinant protein production, antibody humanization and engineering, or cell line development, ATUM delivers solutions to advance your research and speed your path to market.ABOUT US - ATUM
ATUM is based in the US, with a global customer base encompassing academia, government and the pharmaceutical, chemical, agricultural and biotechnology industries. ATUM is by far the most published synthetic biology vendor, providing expert support to and collaboration with scientists.PROTEINS - ATUM
We also offer Angiotensin Converting Enzyme (ACE-2) fused to human or murine Fc. ACE-2 is the host cell receptor responsible for mediating infection by SARS-CoV-2. For all other SARS-CoV-2/COVID-19 related proteins and stable pools, please inquire: email: info@atum.bio or call us 1-650-853-8347 x200.MAMMALIAN - ATUM
Mammalian cells are frequently used to produce secreted proteins. However, the effectiveness of a mammalian secretion signal sequence is highly dependent on the protein being secreted: signal sequences that work well for one chain of an antibody may be mediocre for another.PICHIA - ATUM
Cells are frozen on a dry ice/ethanol bath and stored at -80°C. Pichia Culture and Induction Protocol from ATUM. Notes. These strains have been jointly developed by BioGrammatics Inc. and ATUM. Proteins and peptides made with ATUM catalog Pichia strains (the “Pichia Strains”) can be used commercially without license obligations.GRNA DESIGN
The ATUM gRNA Design Tool enables: Suppression of off-target effects by avoiding sequences that match elsewhere in the genome. Direct ordering of your selected gRNAs in Electra CRISPR vectors, Transfection Ready. Enter your target gene name or your target sequence, and the design tool will identify all Cas9 target sites within the input sequence. CRISPR GRNA DESIGN TOOL CRISPR gRNA Design tool. Please wait, searching The ATUM gRNA Design Tool is provided "as is", with no explicit or implicit guarantees for any purpose. ATUM cannot guarantee the performance of any individual gRNA designed using the Tool. Custom CRISPR Constructs and CRISPR Catalog Vectors are not for use in humans.PROTEIN PAINT BOX
ProteinPaintbox® Synthetic non-Aequorea fluorescent and chromogenic proteins bring a world of color to your research.Overview. ATUM's synthetic fluorescent and chromogenic proteins (non-Aequorea) are an ideal source of protein coding sequences (genes) that can be easily excised using the flanking restriction sites and cloned into any other expression vector of choice. EXPRESSION SOLUTIONS expression Expression of active protein at high levels requires selecting the best coding sequence for a gene and the best vector fromwhich to express
CELL LINE DEVELOPMENT 2 2181 cell line development process Coding sequence optimization Expression of proteins in mammalian cells may be limited by codon usage and secretion signals.ABOUT US - ATUM
ATUM is based in the US, with a global customer base encompassing academia, government and the pharmaceutical, chemical, agricultural and biotechnology industries. ATUM is by far the most published synthetic biology vendor, providing expert support to and collaboration with scientists.PROTEINS - ATUM
We also offer Angiotensin Converting Enzyme (ACE-2) fused to human or murine Fc. ACE-2 is the host cell receptor responsible for mediating infection by SARS-CoV-2. For all other SARS-CoV-2/COVID-19 related proteins and stable pools, please inquire: email: info@atum.bio or call us 1-650-853-8347 x200.ELECTRA SYSTEM
ATUM has developed a simple, one-tube, universal cloning process that can be performed in a 5 minute bench-top reaction with the fidelity of a restriction based cloning system. The Electra system uses the type IIS restriction enzyme SapI, which recognizes a 7bp non-palindromic recognition sequence and leaves a 3bp 5’ overhang after digestion. BACTERIAL EXPRESSION VECTORS ATUM can even test your gene for high expression. Expression Test is now available for bacterial vector panels with a volume discount. Cost for expression testing is $395.00 USD per gene per vector with an additional 10% off the total. Prices are in addition to regular genesynthesis fees.
CELL LINE DEVELOPMENT ATUM's Leap-In transposases and synthetic transposons enable a cost-effective, easily implemented cell line development workflow. The technology delivers high productivity pools and cell lines, resulting in consistent product quality. The Leap-In technology underpins ATUM's cell line development services and is also licensable for yourin-house
PICHIA - ATUM
Cells are frozen on a dry ice/ethanol bath and stored at -80°C. Pichia Culture and Induction Protocol from ATUM. Notes. These strains have been jointly developed by BioGrammatics Inc. and ATUM. Proteins and peptides made with ATUM catalog Pichia strains (the “Pichia Strains”) can be used commercially without license obligations.CRISPR - ATUM
Overview - CRISPR Vectors. ATUM’s CRISPR vectors comprise a gene encoding the Cas9 nuclease, and a cassette for expressing the guide RNA which targets the nuclease. Cas9 nucleases are directed by a 20nt guide sequence to cleave almost any genomic locus. The resulting chromosomal break will normally be repaired via non-homologous endjoining
MAMMALIAN EXPRESSION VECTORS ATUM′s pD600 series of mammalian transient expression vectors are still available. These vectors use the strong CMV/IE enhancer/promoter and an SV40 replication origin to give high levels of protein expression in mammalian cells. Although most pD600s have mammalian selectable markers, we recommend the Bifunctional Leap-In® vectorsfor
EXPRESSION SOLUTIONS expression Expression of active protein at high levels requires selecting the best coding sequence for a gene and the best vector fromwhich to express
YEAST - ATUM
protein expression in yeast. Yeast can be used to express secreted and cytoplasmic proteins. The methylotrophic yeast Pichia pastoris is particularly advantageous for producing secreted proteins including those containing disulfide bonds. ATUM offers protein production services in P. pastoris strains, and in Saccharomyces cerevisiae, or we can provide vectors for yeast expression in yeast in HOME - ATUMCELL LINE DEVELOPMENTPROTEIN PAINTBOXPROTEIN SERVICESABOUT USCAREERSGPS TECHNOLOGY A Fully Integrated Ecosystem. Every path to discovery is different. We focus on your goals and work hand in hand with you to achieve them. Whether you need expression optimization, recombinant protein production, antibody humanization and engineering, or cell line development, ATUM delivers solutions to advance your research and speed your path to market.ABOUT US - ATUM
ATUM is based in the US, with a global customer base encompassing academia, government and the pharmaceutical, chemical, agricultural and biotechnology industries. ATUM is by far the most published synthetic biology vendor, providing expert support to and collaboration with scientists.ELECTRA SYSTEM
ATUM has developed a simple, one-tube, universal cloning process that can be performed in a 5 minute bench-top reaction with the fidelity of a restriction based cloning system. The Electra system uses the type IIS restriction enzyme SapI, which recognizes a 7bp non-palindromic recognition sequence and leaves a 3bp 5’ overhang after digestion. BACTERIAL EXPRESSION VECTORS ATUM can even test your gene for high expression. Expression Test is now available for bacterial vector panels with a volume discount. Cost for expression testing is $395.00 USD per gene per vector with an additional 10% off the total. Prices are in addition to regular genesynthesis fees.
PICHIA - ATUM
Cells are frozen on a dry ice/ethanol bath and stored at -80°C. Pichia Culture and Induction Protocol from ATUM. Notes. These strains have been jointly developed by BioGrammatics Inc. and ATUM. Proteins and peptides made with ATUM catalog Pichia strains (the “Pichia Strains”) can be used commercially without license obligations. CELL LINE DEVELOPMENT ATUM's Leap-In transposases and synthetic transposons enable a cost-effective, easily implemented cell line development workflow. The technology delivers high productivity pools and cell lines, resulting in consistent product quality. The Leap-In technology underpins ATUM's cell line development services and is also licensable for yourin-house
MAMMALIAN - ATUM
Mammalian cells are frequently used to produce secreted proteins. However, the effectiveness of a mammalian secretion signal sequence is highly dependent on the protein being secreted: signal sequences that work well for one chain of an antibody may be mediocre for another. CRISPR GRNA DESIGN TOOL CRISPR gRNA Design tool. Please wait, searching The ATUM gRNA Design Tool is provided "as is", with no explicit or implicit guarantees for any purpose. ATUM cannot guarantee the performance of any individual gRNA designed using the Tool. Custom CRISPR Constructs and CRISPR Catalog Vectors are not for use in humans.PROTEIN PAINT BOX
ProteinPaintbox® Synthetic non-Aequorea fluorescent and chromogenic proteins bring a world of color to your research.Overview. ATUM's synthetic fluorescent and chromogenic proteins (non-Aequorea) are an ideal source of protein coding sequences (genes) that can be easily excised using the flanking restriction sites and cloned into any other expression vector of choice. CELL LINE DEVELOPMENT 2 2181 cell line development process Coding sequence optimization Expression of proteins in mammalian cells may be limited by codon usage and secretion signals. HOME - ATUMCELL LINE DEVELOPMENTPROTEIN PAINTBOXPROTEIN SERVICESABOUT USCAREERSGPS TECHNOLOGY A Fully Integrated Ecosystem. Every path to discovery is different. We focus on your goals and work hand in hand with you to achieve them. Whether you need expression optimization, recombinant protein production, antibody humanization and engineering, or cell line development, ATUM delivers solutions to advance your research and speed your path to market.ABOUT US - ATUM
ATUM is based in the US, with a global customer base encompassing academia, government and the pharmaceutical, chemical, agricultural and biotechnology industries. ATUM is by far the most published synthetic biology vendor, providing expert support to and collaboration with scientists.ELECTRA SYSTEM
ATUM has developed a simple, one-tube, universal cloning process that can be performed in a 5 minute bench-top reaction with the fidelity of a restriction based cloning system. The Electra system uses the type IIS restriction enzyme SapI, which recognizes a 7bp non-palindromic recognition sequence and leaves a 3bp 5’ overhang after digestion. BACTERIAL EXPRESSION VECTORS ATUM can even test your gene for high expression. Expression Test is now available for bacterial vector panels with a volume discount. Cost for expression testing is $395.00 USD per gene per vector with an additional 10% off the total. Prices are in addition to regular genesynthesis fees.
PICHIA - ATUM
Cells are frozen on a dry ice/ethanol bath and stored at -80°C. Pichia Culture and Induction Protocol from ATUM. Notes. These strains have been jointly developed by BioGrammatics Inc. and ATUM. Proteins and peptides made with ATUM catalog Pichia strains (the “Pichia Strains”) can be used commercially without license obligations. CELL LINE DEVELOPMENT ATUM's Leap-In transposases and synthetic transposons enable a cost-effective, easily implemented cell line development workflow. The technology delivers high productivity pools and cell lines, resulting in consistent product quality. The Leap-In technology underpins ATUM's cell line development services and is also licensable for yourin-house
MAMMALIAN - ATUM
Mammalian cells are frequently used to produce secreted proteins. However, the effectiveness of a mammalian secretion signal sequence is highly dependent on the protein being secreted: signal sequences that work well for one chain of an antibody may be mediocre for another. CRISPR GRNA DESIGN TOOL CRISPR gRNA Design tool. Please wait, searching The ATUM gRNA Design Tool is provided "as is", with no explicit or implicit guarantees for any purpose. ATUM cannot guarantee the performance of any individual gRNA designed using the Tool. Custom CRISPR Constructs and CRISPR Catalog Vectors are not for use in humans.PROTEIN PAINT BOX
ProteinPaintbox® Synthetic non-Aequorea fluorescent and chromogenic proteins bring a world of color to your research.Overview. ATUM's synthetic fluorescent and chromogenic proteins (non-Aequorea) are an ideal source of protein coding sequences (genes) that can be easily excised using the flanking restriction sites and cloned into any other expression vector of choice. CELL LINE DEVELOPMENT 2 2181 cell line development process Coding sequence optimization Expression of proteins in mammalian cells may be limited by codon usage and secretion signals. MAMMALIAN EXPRESSION VECTORS ATUM′s pD600 series of mammalian transient expression vectors are still available. These vectors use the strong CMV/IE enhancer/promoter and an SV40 replication origin to give high levels of protein expression in mammalian cells. Although most pD600s have mammalian selectable markers, we recommend the Bifunctional Leap-In® vectorsfor
PROTEINS - ATUM
We also offer Angiotensin Converting Enzyme (ACE-2) fused to human or murine Fc. ACE-2 is the host cell receptor responsible for mediating infection by SARS-CoV-2. For all other SARS-CoV-2/COVID-19 related proteins and stable pools, please inquire: email: info@atum.bio or call us 1-650-853-8347 x200. YEAST EXPRESSION VECTORS The methylotrophic yeast Pichia pastoris is a useful system for the expression of milligram-to-gram quantities of protein for both basic laboratory research and industrial manufacturing. ATUM offers Pichia vectors with a choice of promoters for cytoplasmic or secreted expression. The major advantage of expressing secreted heterologousprotein
BACTERIAL - ATUM
Open Reading Frame. Our earliest work in developing our GeneGPS® design platform was performed in E. coli.We have subsequently applied GeneGPS® to optimize genes for expression in a range of bacterial hosts including Bacillus and Clostridium.By designing and testing hundreds of genes and modelling the data, we are able to identify the critical sequence parameters that most strongly influenceGRNA DESIGN
The ATUM gRNA Design Tool enables: Suppression of off-target effects by avoiding sequences that match elsewhere in the genome. Direct ordering of your selected gRNAs in Electra CRISPR vectors, Transfection Ready. Enter your target gene name or your target sequence, and the design tool will identify all Cas9 target sites within the input sequence.CRISPR - ATUM
Overview - CRISPR Vectors. ATUM’s CRISPR vectors comprise a gene encoding the Cas9 nuclease, and a cassette for expressing the guide RNA which targets the nuclease. Cas9 nucleases are directed by a 20nt guide sequence to cleave almost any genomic locus. The resulting chromosomal break will normally be repaired via non-homologous endjoining
EXPRESSION SOLUTIONS expression Expression of active protein at high levels requires selecting the best coding sequence for a gene and the best vector fromwhich to express
GENE DESIGNER
The DNA design tool for tomorrow’s scientists. The ideal software developed to design sequences de novo can easily be incorporated into Synthetic Biology, Systems Biology, Bioengineering and BioInformatics curricula. Gene Designer 2.0 encompasses a complete and visually rich set of tools to bring student's creative ideas to completion. CELL LINE DEVELOPMENT 2 2181 cell line development process Coding sequence optimization Expression of proteins in mammalian cells may be limited by codon usage and secretion signals. NOVEL TRANSPOSASE TOOLS FOR CELL ENGINEERING Novel Transposase Tools for Cell Engineering Ferenc Boldog, Ph.D ATUM June 12, 2018 Cell Line Development & Engineering HOME - ATUMCELL LINE DEVELOPMENTPROTEIN PAINTBOXPROTEIN SERVICESABOUT USCAREERSGPS TECHNOLOGY A Fully Integrated Ecosystem. Every path to discovery is different. We focus on your goals and work hand in hand with you to achieve them. Whether you need expression optimization, recombinant protein production, antibody humanization and engineering, or cell line development, ATUM delivers solutions to advance your research and speed your path to market.ABOUT US - ATUM
ATUM is based in the US, with a global customer base encompassing academia, government and the pharmaceutical, chemical, agricultural and biotechnology industries. ATUM is by far the most published synthetic biology vendor, providing expert support to and collaboration with scientists.ELECTRA SYSTEM
ATUM has developed a simple, one-tube, universal cloning process that can be performed in a 5 minute bench-top reaction with the fidelity of a restriction based cloning system. The Electra system uses the type IIS restriction enzyme SapI, which recognizes a 7bp non-palindromic recognition sequence and leaves a 3bp 5’ overhang after digestion. BACTERIAL EXPRESSION VECTORS ATUM can even test your gene for high expression. Expression Test is now available for bacterial vector panels with a volume discount. Cost for expression testing is $395.00 USD per gene per vector with an additional 10% off the total. Prices are in addition to regular genesynthesis fees.
PICHIA - ATUM
Cells are frozen on a dry ice/ethanol bath and stored at -80°C. Pichia Culture and Induction Protocol from ATUM. Notes. These strains have been jointly developed by BioGrammatics Inc. and ATUM. Proteins and peptides made with ATUM catalog Pichia strains (the “Pichia Strains”) can be used commercially without license obligations. CELL LINE DEVELOPMENT ATUM's Leap-In transposases and synthetic transposons enable a cost-effective, easily implemented cell line development workflow. The technology delivers high productivity pools and cell lines, resulting in consistent product quality. The Leap-In technology underpins ATUM's cell line development services and is also licensable for yourin-house
MAMMALIAN - ATUM
Mammalian cells are frequently used to produce secreted proteins. However, the effectiveness of a mammalian secretion signal sequence is highly dependent on the protein being secreted: signal sequences that work well for one chain of an antibody may be mediocre for another. CRISPR GRNA DESIGN TOOL CRISPR gRNA Design tool. Please wait, searching The ATUM gRNA Design Tool is provided "as is", with no explicit or implicit guarantees for any purpose. ATUM cannot guarantee the performance of any individual gRNA designed using the Tool. Custom CRISPR Constructs and CRISPR Catalog Vectors are not for use in humans.PROTEIN PAINT BOX
ProteinPaintbox® Synthetic non-Aequorea fluorescent and chromogenic proteins bring a world of color to your research.Overview. ATUM's synthetic fluorescent and chromogenic proteins (non-Aequorea) are an ideal source of protein coding sequences (genes) that can be easily excised using the flanking restriction sites and cloned into any other expression vector of choice. CELL LINE DEVELOPMENT 2 2181 cell line development process Coding sequence optimization Expression of proteins in mammalian cells may be limited by codon usage and secretion signals. HOME - ATUMCELL LINE DEVELOPMENTPROTEIN PAINTBOXPROTEIN SERVICESABOUT USCAREERSGPS TECHNOLOGY A Fully Integrated Ecosystem. Every path to discovery is different. We focus on your goals and work hand in hand with you to achieve them. Whether you need expression optimization, recombinant protein production, antibody humanization and engineering, or cell line development, ATUM delivers solutions to advance your research and speed your path to market.ABOUT US - ATUM
ATUM is based in the US, with a global customer base encompassing academia, government and the pharmaceutical, chemical, agricultural and biotechnology industries. ATUM is by far the most published synthetic biology vendor, providing expert support to and collaboration with scientists.ELECTRA SYSTEM
ATUM has developed a simple, one-tube, universal cloning process that can be performed in a 5 minute bench-top reaction with the fidelity of a restriction based cloning system. The Electra system uses the type IIS restriction enzyme SapI, which recognizes a 7bp non-palindromic recognition sequence and leaves a 3bp 5’ overhang after digestion. BACTERIAL EXPRESSION VECTORS ATUM can even test your gene for high expression. Expression Test is now available for bacterial vector panels with a volume discount. Cost for expression testing is $395.00 USD per gene per vector with an additional 10% off the total. Prices are in addition to regular genesynthesis fees.
PICHIA - ATUM
Cells are frozen on a dry ice/ethanol bath and stored at -80°C. Pichia Culture and Induction Protocol from ATUM. Notes. These strains have been jointly developed by BioGrammatics Inc. and ATUM. Proteins and peptides made with ATUM catalog Pichia strains (the “Pichia Strains”) can be used commercially without license obligations. CELL LINE DEVELOPMENT ATUM's Leap-In transposases and synthetic transposons enable a cost-effective, easily implemented cell line development workflow. The technology delivers high productivity pools and cell lines, resulting in consistent product quality. The Leap-In technology underpins ATUM's cell line development services and is also licensable for yourin-house
MAMMALIAN - ATUM
Mammalian cells are frequently used to produce secreted proteins. However, the effectiveness of a mammalian secretion signal sequence is highly dependent on the protein being secreted: signal sequences that work well for one chain of an antibody may be mediocre for another. CRISPR GRNA DESIGN TOOL CRISPR gRNA Design tool. Please wait, searching The ATUM gRNA Design Tool is provided "as is", with no explicit or implicit guarantees for any purpose. ATUM cannot guarantee the performance of any individual gRNA designed using the Tool. Custom CRISPR Constructs and CRISPR Catalog Vectors are not for use in humans.PROTEIN PAINT BOX
ProteinPaintbox® Synthetic non-Aequorea fluorescent and chromogenic proteins bring a world of color to your research.Overview. ATUM's synthetic fluorescent and chromogenic proteins (non-Aequorea) are an ideal source of protein coding sequences (genes) that can be easily excised using the flanking restriction sites and cloned into any other expression vector of choice. CELL LINE DEVELOPMENT 2 2181 cell line development process Coding sequence optimization Expression of proteins in mammalian cells may be limited by codon usage and secretion signals. MAMMALIAN EXPRESSION VECTORS ATUM′s pD600 series of mammalian transient expression vectors are still available. These vectors use the strong CMV/IE enhancer/promoter and an SV40 replication origin to give high levels of protein expression in mammalian cells. Although most pD600s have mammalian selectable markers, we recommend the Bifunctional Leap-In® vectorsfor
PROTEINS - ATUM
We also offer Angiotensin Converting Enzyme (ACE-2) fused to human or murine Fc. ACE-2 is the host cell receptor responsible for mediating infection by SARS-CoV-2. For all other SARS-CoV-2/COVID-19 related proteins and stable pools, please inquire: email: info@atum.bio or call us 1-650-853-8347 x200. YEAST EXPRESSION VECTORS The methylotrophic yeast Pichia pastoris is a useful system for the expression of milligram-to-gram quantities of protein for both basic laboratory research and industrial manufacturing. ATUM offers Pichia vectors with a choice of promoters for cytoplasmic or secreted expression. The major advantage of expressing secreted heterologousprotein
BACTERIAL - ATUM
Open Reading Frame. Our earliest work in developing our GeneGPS® design platform was performed in E. coli.We have subsequently applied GeneGPS® to optimize genes for expression in a range of bacterial hosts including Bacillus and Clostridium.By designing and testing hundreds of genes and modelling the data, we are able to identify the critical sequence parameters that most strongly influenceGRNA DESIGN
The ATUM gRNA Design Tool enables: Suppression of off-target effects by avoiding sequences that match elsewhere in the genome. Direct ordering of your selected gRNAs in Electra CRISPR vectors, Transfection Ready. Enter your target gene name or your target sequence, and the design tool will identify all Cas9 target sites within the input sequence.CRISPR - ATUM
Overview - CRISPR Vectors. ATUM’s CRISPR vectors comprise a gene encoding the Cas9 nuclease, and a cassette for expressing the guide RNA which targets the nuclease. Cas9 nucleases are directed by a 20nt guide sequence to cleave almost any genomic locus. The resulting chromosomal break will normally be repaired via non-homologous endjoining
EXPRESSION SOLUTIONS expression Expression of active protein at high levels requires selecting the best coding sequence for a gene and the best vector fromwhich to express
GENE DESIGNER
The DNA design tool for tomorrow’s scientists. The ideal software developed to design sequences de novo can easily be incorporated into Synthetic Biology, Systems Biology, Bioengineering and BioInformatics curricula. Gene Designer 2.0 encompasses a complete and visually rich set of tools to bring student's creative ideas to completion. CELL LINE DEVELOPMENT 2 2181 cell line development process Coding sequence optimization Expression of proteins in mammalian cells may be limited by codon usage and secretion signals. NOVEL TRANSPOSASE TOOLS FOR CELL ENGINEERING Novel Transposase Tools for Cell Engineering Ferenc Boldog, Ph.D ATUM June 12, 2018 Cell Line Development & Engineering HOME - ATUMCELL LINE DEVELOPMENTPROTEIN PAINTBOXPROTEIN SERVICESABOUT USCAREERSGPS TECHNOLOGY A Fully Integrated Ecosystem. Every path to discovery is different. We focus on your goals and work hand in hand with you to achieve them. Whether you need expression optimization, recombinant protein production, antibody humanization and engineering, or cell line development, ATUM delivers solutions to advance your research and speed your path to market.ABOUT US - ATUM
ATUM is based in the US, with a global customer base encompassing academia, government and the pharmaceutical, chemical, agricultural and biotechnology industries. ATUM is by far the most published synthetic biology vendor, providing expert support to and collaboration with scientists.ELECTRA SYSTEM
ATUM has developed a simple, one-tube, universal cloning process that can be performed in a 5 minute bench-top reaction with the fidelity of a restriction based cloning system. The Electra system uses the type IIS restriction enzyme SapI, which recognizes a 7bp non-palindromic recognition sequence and leaves a 3bp 5’ overhang after digestion.PICHIA - ATUM
Cells are frozen on a dry ice/ethanol bath and stored at -80°C. Pichia Culture and Induction Protocol from ATUM. Notes. These strains have been jointly developed by BioGrammatics Inc. and ATUM. Proteins and peptides made with ATUM catalog Pichia strains (the “Pichia Strains”) can be used commercially without license obligations.LEAP-IN - ATUM
Leap-In Transposase®. ATUM's Leap-In Transposases® and synthetic transposons enable a cost-effective, easily implemented cell line development workflow, delivering high productivity pools and cell lines with consistent product quality. Take a look at howGRNA DESIGN
The ATUM gRNA Design Tool enables: Suppression of off-target effects by avoiding sequences that match elsewhere in the genome. Direct ordering of your selected gRNAs in Electra CRISPR vectors, Transfection Ready. Enter your target gene name or your target sequence, and the design tool will identify all Cas9 target sites within the input sequence.MAMMALIAN - ATUM
Mammalian cells are frequently used to produce secreted proteins. However, the effectiveness of a mammalian secretion signal sequence is highly dependent on the protein being secreted: signal sequences that work well for one chain of an antibody may be mediocre for another. CELL LINE DEVELOPMENT ATUM's Leap-In transposases and synthetic transposons enable a cost-effective, easily implemented cell line development workflow. The technology delivers high productivity pools and cell lines, resulting in consistent product quality. The Leap-In technology underpins ATUM's cell line development services and is also licensable for yourin-house
CRISPR GRNA DESIGN TOOL CRISPR gRNA Design tool. Please wait, searching The ATUM gRNA Design Tool is provided "as is", with no explicit or implicit guarantees for any purpose. ATUM cannot guarantee the performance of any individual gRNA designed using the Tool. Custom CRISPR Constructs and CRISPR Catalog Vectors are not for use in humans.PROTEIN PAINT BOX
ProteinPaintbox® Synthetic non-Aequorea fluorescent and chromogenic proteins bring a world of color to your research.Overview. ATUM's synthetic fluorescent and chromogenic proteins (non-Aequorea) are an ideal source of protein coding sequences (genes) that can be easily excised using the flanking restriction sites and cloned into any other expression vector of choice. HOME - ATUMCELL LINE DEVELOPMENTPROTEIN PAINTBOXPROTEIN SERVICESABOUT USCAREERSGPS TECHNOLOGY A Fully Integrated Ecosystem. Every path to discovery is different. We focus on your goals and work hand in hand with you to achieve them. Whether you need expression optimization, recombinant protein production, antibody humanization and engineering, or cell line development, ATUM delivers solutions to advance your research and speed your path to market.ABOUT US - ATUM
ATUM is based in the US, with a global customer base encompassing academia, government and the pharmaceutical, chemical, agricultural and biotechnology industries. ATUM is by far the most published synthetic biology vendor, providing expert support to and collaboration with scientists.ELECTRA SYSTEM
ATUM has developed a simple, one-tube, universal cloning process that can be performed in a 5 minute bench-top reaction with the fidelity of a restriction based cloning system. The Electra system uses the type IIS restriction enzyme SapI, which recognizes a 7bp non-palindromic recognition sequence and leaves a 3bp 5’ overhang after digestion.PICHIA - ATUM
Cells are frozen on a dry ice/ethanol bath and stored at -80°C. Pichia Culture and Induction Protocol from ATUM. Notes. These strains have been jointly developed by BioGrammatics Inc. and ATUM. Proteins and peptides made with ATUM catalog Pichia strains (the “Pichia Strains”) can be used commercially without license obligations.LEAP-IN - ATUM
Leap-In Transposase®. ATUM's Leap-In Transposases® and synthetic transposons enable a cost-effective, easily implemented cell line development workflow, delivering high productivity pools and cell lines with consistent product quality. Take a look at howGRNA DESIGN
The ATUM gRNA Design Tool enables: Suppression of off-target effects by avoiding sequences that match elsewhere in the genome. Direct ordering of your selected gRNAs in Electra CRISPR vectors, Transfection Ready. Enter your target gene name or your target sequence, and the design tool will identify all Cas9 target sites within the input sequence.MAMMALIAN - ATUM
Mammalian cells are frequently used to produce secreted proteins. However, the effectiveness of a mammalian secretion signal sequence is highly dependent on the protein being secreted: signal sequences that work well for one chain of an antibody may be mediocre for another. CELL LINE DEVELOPMENT ATUM's Leap-In transposases and synthetic transposons enable a cost-effective, easily implemented cell line development workflow. The technology delivers high productivity pools and cell lines, resulting in consistent product quality. The Leap-In technology underpins ATUM's cell line development services and is also licensable for yourin-house
CRISPR GRNA DESIGN TOOL CRISPR gRNA Design tool. Please wait, searching The ATUM gRNA Design Tool is provided "as is", with no explicit or implicit guarantees for any purpose. ATUM cannot guarantee the performance of any individual gRNA designed using the Tool. Custom CRISPR Constructs and CRISPR Catalog Vectors are not for use in humans.PROTEIN PAINT BOX
ProteinPaintbox® Synthetic non-Aequorea fluorescent and chromogenic proteins bring a world of color to your research.Overview. ATUM's synthetic fluorescent and chromogenic proteins (non-Aequorea) are an ideal source of protein coding sequences (genes) that can be easily excised using the flanking restriction sites and cloned into any other expression vector of choice.ABOUT US - ATUM
ATUM is based in the US, with a global customer base encompassing academia, government and the pharmaceutical, chemical, agricultural and biotechnology industries. ATUM is by far the most published synthetic biology vendor, providing expert support to and collaboration with scientists.PROTEINS - ATUM
We also offer Angiotensin Converting Enzyme (ACE-2) fused to human or murine Fc. ACE-2 is the host cell receptor responsible for mediating infection by SARS-CoV-2. For all other SARS-CoV-2/COVID-19 related proteins and stable pools, please inquire: email: info@atum.bio or call us 1-650-853-8347 x200. BACTERIAL EXPRESSION VECTORS ATUM can even test your gene for high expression. Expression Test is now available for bacterial vector panels with a volume discount. Cost for expression testing is $395.00 USD per gene per vector with an additional 10% off the total. Prices are in addition to regular genesynthesis fees.
PICHIA - ATUM
Cells are frozen on a dry ice/ethanol bath and stored at -80°C. Pichia Culture and Induction Protocol from ATUM. Notes. These strains have been jointly developed by BioGrammatics Inc. and ATUM. Proteins and peptides made with ATUM catalog Pichia strains (the “Pichia Strains”) can be used commercially without license obligations.CRISPR - ATUM
Overview - CRISPR Vectors. ATUM’s CRISPR vectors comprise a gene encoding the Cas9 nuclease, and a cassette for expressing the guide RNA which targets the nuclease. Cas9 nucleases are directed by a 20nt guide sequence to cleave almost any genomic locus. The resulting chromosomal break will normally be repaired via non-homologous endjoining
MAMMALIAN EXPRESSION VECTORS ATUM′s pD600 series of mammalian transient expression vectors are still available. These vectors use the strong CMV/IE enhancer/promoter and an SV40 replication origin to give high levels of protein expression in mammalian cells. Although most pD600s have mammalian selectable markers, we recommend the Bifunctional Leap-In® vectorsfor
SIGN IN TO MY ACCOUNT Hello! Please sign in below Email address. PasswordGPS TECHNOLOGY
gps. technology. ATUM's GPS technology platform combines design of experiment, accurate empirical measurement and machine learning tools. At its core, GPS enables us to test small numbers of variants for commercially relevant properties, and to rapidly create molecules that work well in the real world. GENE DESIGNER BY DNA 2.0 TUTORIAL 2 Getting Started -Go to https://www.dna20.com -Under the tab Scientific Resources, select Gene Designer-Click on the Download Now icon -Create an Account -Click on the Install Now icon -An activation key will be sent to your email address. NOVEL TRANSPOSASE TOOLS FOR CELL ENGINEERING Novel Transposase Tools for Cell Engineering Ferenc Boldog, Ph.D ATUM June 12, 2018 Cell Line Development & EngineeringWhat we do
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A Fully Integrated Ecosystem Every path to discovery is different. We focus on your goals and work hand in hand with you to achieve them. Whether you need expression optimization, recombinant protein production, antibody humanization and engineering, or cell line development, ATUM delivers solutions to advance your research and speed your path to market.Cell Line
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A FULLY INTEGRATED ECOSYSTEM Every path to discovery is different. We focus on your goals and work hand in hand with you to achieve them. Whether you need expression optimization, recombinant protein production, antibody humanization and engineering, or cell line development, ATUM delivers solutions to advance your research and speed your path to market. ATUM'S GPS PLATFORM - THE KEY TO YOUR SUCCESS Our GPS® technology platform combines design of experiment (DoE) with machine learning tools, allowing us to optimize your biological systems for the properties that matter in the real world. The GPS technology provides an integrated bioengineering suite of solutions for the design and manufacture of optimized genes, vectors, enzymes, antibodies and cell lines with application across the Life Sciencespectrum.
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We think of projects more as a collaboration than services. We aim to combine your expertise with our expertise so that together we succeed. Whether you are selecting the best vector and codon optimization for a gene to express in your own lab, or you want us to take you from hybridoma sequence to high producing cell line, our team is ready tohelp.
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ATUM will present on Friday, June 9 at 1:30pm GMT, 4:30am PST September 13-16 2021 15TH PEACE PROTEIN EXPRESSION IN ANIMAL CELLS CONFERENCE, VIRTUALVirtual
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9TH ANNUAL CELL LINE DEVELOPMENT & ENGINEERING ASIAShanghai, China
ATUM will present and exhibit news mentions and press releases2021-03-19
FIGHTING FOR OUR LIVESDiablo magazine
2021-03-19
INOM EN TIMME HADE VI TIO RUSH-ORDERS Forskning o Framsteg2020-07-26
BIOTECH FIRM REBOUNDS TO COMBAT CORONAVIRUS San Francisco Chronicle2020-07-22
BIG HEROES OF SMALL BUSINESS: THIS BIOTECH BUSINESS WON'T LET COST GET IN THE WAY OF A COVID CUREInc.
2020-07-06
DEBOTTLENECKING OPPORTUNITIES CLEARER WITH A BIOPROCESSING 4.0PERSPECTIVE
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2020-10-22
IAVI, MERCK KGAA, DARMSTADT, GERMANY, AND SERUM INSTITUTE OF INDIA JOIN FORCES TO DEVELOP MONOCLONAL ANTIBODIES FOR COVID-19 AND ENSURE PROMPT AND EQUITABLE GLOBAL ACCESSRead
2020-10-14
RENTSCHLER BIOPHARMA AND ATUM ANNOUNCE LEAP-IN TRANSPOSASE® LICENSING AGREEMENT FOR DEVELOPMENT OF ROBUST MANUFACTURING PROCESSES FOR COMPLEX THERAPEUTIC PROTEINSRead
2020-06-15
ATUM AND PHYLEX BIOSCIENCES COLLABORATE TO ACCELERATE THE DEVELOPMENT OF A UNIVERSAL SARS CORONAVIRUS VACCINERead
2020-04-20
SOLENTIM AND ATUM TO BUNDLE LEAP-IN TRANSPOSASE® WITH THE VIPS™ SINGLE CELL CLONING INSTRUMENT FOR CELL LINE DEVELOPMENTRead
2020-03-25
ATUM PROVIDES COVID-19 DIAGNOSTIC AND THERAPEUTIC DEVELOPERS FREE ACCESS TO ITS PROTEIN SYNTHESIS PLATFORMRead `
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