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CENTERPLASMIDS
Addgene's ready-to-use AAV preparations include tools for serotype testing, optogenetics, chemogenetics, biosensors, retrograde AAV, and more. Addgene's COVID-19 collection includes plasmids expressing SARS-CoV-2 open reading frames, ACE2, TMPRSS2, FURIN, and more. Addgene's repository contains over 90,000 plasmids.TLCV2 - ADDGENE
Plasmid TLCV2 from Dr. Adam Karpf's lab contains the insert Cas9-2A-eGFP and is published in Cancers (Basel). 2019 Feb 21;11(2). pii: cancers11020251. doi: 10.3390/cancers11020251. This plasmid is available through Addgene.ADDGENE: PINDUCER20
Plasmid pInducer20 from Dr. Stephen Elledge's lab is published in Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 10.1073/pnas.1019736108. Epub 2011 Feb 9. ADDGENE: PINDUCER10-MIR-RUP-PHES Plasmid pInducer10-mir-RUP-PheS from Dr. Stephen Elledge's lab contains the insert PheS Gly294 and is published in Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 10.1073/pnas.1019736108. Epub 2011 Feb 9. This plasmid is available through Addgene.ADDGENE: PPD95_75
Image: Illustrated plasmid map in PNG format GenBank File: Plasmid sequence and annotations. Use text editor or plasmid mapping software to view sequence. SnapGene File: Plasmid sequence and SnapGene enhanced annotations. Use with SnapGene software or the free Viewer to visualize additional data and align other sequences.PX551 - ADDGENE
Plasmid PX551 from Dr. Feng Zhang's lab contains the insert SpCas9 and is published in Nat Biotechnol. 2014 Oct 19. doi: 10.1038/nbt.3055. This plasmid is available through Addgene. ADDGENE: MITO-V5-APEX2 Plasmid mito-V5-APEX2 from Dr. Alice Ting's lab contains the insert APEX2 and is published in Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179. This plasmid is available through Addgene. ADDGENE: VECTOR DATABASE Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene. This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.ADDGENE: PTF101.1
Plasmid pTF101.1 from Dr. Kan Wang's lab is published in Euphytica (2004) 136: 167. This plasmid is available through Addgene. ADDGENE: PCMV DELTA R8.2 Plasmid pCMV delta R8.2 from Dr. Didier Trono's lab contains the insert HIV-1 GAG/POL, Tat and Rev. This plasmid is available throughAddgene.
ADDGENE: HOMEPAGELOG INBROWSE CATALOGDEPOSITEDUCATION & TOOLSHELPCENTERPLASMIDS
Addgene's ready-to-use AAV preparations include tools for serotype testing, optogenetics, chemogenetics, biosensors, retrograde AAV, and more. Addgene's COVID-19 collection includes plasmids expressing SARS-CoV-2 open reading frames, ACE2, TMPRSS2, FURIN, and more. Addgene's repository contains over 90,000 plasmids.TLCV2 - ADDGENE
Plasmid TLCV2 from Dr. Adam Karpf's lab contains the insert Cas9-2A-eGFP and is published in Cancers (Basel). 2019 Feb 21;11(2). pii: cancers11020251. doi: 10.3390/cancers11020251. This plasmid is available through Addgene.ADDGENE: PINDUCER20
Plasmid pInducer20 from Dr. Stephen Elledge's lab is published in Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 10.1073/pnas.1019736108. Epub 2011 Feb 9. ADDGENE: PINDUCER10-MIR-RUP-PHES Plasmid pInducer10-mir-RUP-PheS from Dr. Stephen Elledge's lab contains the insert PheS Gly294 and is published in Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 10.1073/pnas.1019736108. Epub 2011 Feb 9. This plasmid is available through Addgene.ADDGENE: PPD95_75
Image: Illustrated plasmid map in PNG format GenBank File: Plasmid sequence and annotations. Use text editor or plasmid mapping software to view sequence. SnapGene File: Plasmid sequence and SnapGene enhanced annotations. Use with SnapGene software or the free Viewer to visualize additional data and align other sequences.PX551 - ADDGENE
Plasmid PX551 from Dr. Feng Zhang's lab contains the insert SpCas9 and is published in Nat Biotechnol. 2014 Oct 19. doi: 10.1038/nbt.3055. This plasmid is available through Addgene. ADDGENE: MITO-V5-APEX2 Plasmid mito-V5-APEX2 from Dr. Alice Ting's lab contains the insert APEX2 and is published in Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179. This plasmid is available through Addgene. ADDGENE: VECTOR DATABASE Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene. This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.ADDGENE: PTF101.1
Plasmid pTF101.1 from Dr. Kan Wang's lab is published in Euphytica (2004) 136: 167. This plasmid is available through Addgene. ADDGENE: PCMV DELTA R8.2 Plasmid pCMV delta R8.2 from Dr. Didier Trono's lab contains the insert HIV-1 GAG/POL, Tat and Rev. This plasmid is available throughAddgene.
ADDGENE
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.ADDGENE: PROTOCOL
After you have bacterial growth, add 500 μL of the overnight culture to 500 μL of 50% glycerol in a 2 mL screw top tube or cryovial and gently mix. Note: Make the 50% glycerol solution by diluting 100% glycerol in dH20. Note: Snap top tubes are not recommended as they can open unexpectedly at -80°C. Freeze the glycerol stock tube at -80°C. ADDGENE: PCAP03-ACC(3)IV This method allows the use of shorter capture arms and conveniently selects against non-homologous end joining in the presence of 5-fluoroorotic acid (5-FOA). In order to easy linearize pCAP03, the 1369 bp apramycin resistance cassette (acc (3)IV) was amplified from pIJ773 and cloned into the pCAP03 to obtain pCAP03-acc (3)IV . Vectortype.
VECTOR DATABASE
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.ADDGENE: PG1
Plasmid pG1 from Dr. Lars Steinmetz's lab contains the insert Tef1-Cas9 with RPL25 Intron and is published in Journal of Microbiology & Biology Education This plasmid is available throughAddgene.
ADDGENE: PET28A GX-NL2 Plasmid pET28a Gx-NL2 from Dr. Maarten Merkx's lab contains the insert protein G - NanoLuc - NanoLuc and is published in Bioconjug Chem. 2020 Mar 18;31(3):656-662. doi: 10.1021/acs.bioconjchem.9b00804. Epub 2020 Jan 22. This plasmid is available through Addgene. ADDGENE: PMRUBY2-CROWDINGLINKER-CLOVER Plasmid pmRuby2-CrowdingLinker-Clover from Dr. Ke Xu's lab contains the insert Crowdedness-sensitive linker domain and is published in Light Sci Appl. 2021 May 8;10(1):97. doi: 10.1038/s41377-021-00536-3. This plasmid is available through Addgene.ADDGENE: PXZX353
Plasmid pXZX353 from Dr. Jef Boeke's lab contains the insert MatA and is published in G3 (Bethesda). 2018 Jan 4;8(1):173-183. doi: 10.1534/g3.117.300347. This plasmid is available through Addgene.ADDGENE: PED1A71
Plasmid pED1a71 from Dr. Kevin Esvelt's lab contains the insert luxAB-357-AGGA and is published in Unpublished This plasmid is available through Addgene.ADDGENE
offering advice on areas where you might need some help.Today in our transferable skills guide: Time Mangement made small accomplishments each day that should help keep you content and give you the energy to finishday, writing your to-do list the night before will ADDGENE: HOMEPAGELOG INBROWSE CATALOGDEPOSITEDUCATION & TOOLSHELPCENTERPLASMIDS
Addgene's ready-to-use AAV preparations include tools for serotype testing, optogenetics, chemogenetics, biosensors, retrograde AAV, and more. Addgene's COVID-19 collection includes plasmids expressing SARS-CoV-2 open reading frames, ACE2, TMPRSS2, FURIN, and more. Addgene's repository contains over 90,000 plasmids.ADDGENE: PINDUCER20
Plasmid pInducer20 from Dr. Stephen Elledge's lab is published in Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 10.1073/pnas.1019736108. Epub 2011 Feb 9.TLCV2 - ADDGENE
Plasmid TLCV2 from Dr. Adam Karpf's lab contains the insert Cas9-2A-eGFP and is published in Cancers (Basel). 2019 Feb 21;11(2). pii: cancers11020251. doi: 10.3390/cancers11020251. This plasmid is available through Addgene.ADDGENE: PTF101.1
Plasmid pTF101.1 from Dr. Kan Wang's lab is published in Euphytica (2004) 136: 167. This plasmid is available through Addgene. ADDGENE: PINDUCER10-MIR-RUP-PHES Plasmid pInducer10-mir-RUP-PheS from Dr. Stephen Elledge's lab contains the insert PheS Gly294 and is published in Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 10.1073/pnas.1019736108. Epub 2011 Feb 9. This plasmid is available through Addgene.PX551 - ADDGENE
Plasmid PX551 from Dr. Feng Zhang's lab contains the insert SpCas9 and is published in Nat Biotechnol. 2014 Oct 19. doi: 10.1038/nbt.3055. This plasmid is available through Addgene.ADDGENE: PRGEB32
Plasmid pRGEB32 from Dr. Yinong Yang's lab is published in Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3570-5. doi: 10.1073/pnas.1420294112. Epub 2015 Mar 2.ADDGENE: PPD95_75
Image: Illustrated plasmid map in PNG format GenBank File: Plasmid sequence and annotations. Use text editor or plasmid mapping software to view sequence. SnapGene File: Plasmid sequence and SnapGene enhanced annotations. Use with SnapGene software or the free Viewer to visualize additional data and align other sequences.ADDGENE: PRGEB31
Plasmid pRGEB31 from Dr. Yinong Yang's lab is published in Mol Plant. 2013 Nov;6(6):1975-83. doi: 10.1093/mp/sst119. Epub 2013 Aug 17. This plasmid is available through Addgene. ADDGENE: PSPCAS9(BB)-2A-GFP (PX458) Plasmid pSpCas9(BB)-2A-GFP (PX458) from Dr. Feng Zhang's lab contains the insert hSpCas9 and is published in Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. This plasmid is available through Addgene. ADDGENE: HOMEPAGELOG INBROWSE CATALOGDEPOSITEDUCATION & TOOLSHELPCENTERPLASMIDS
Addgene's ready-to-use AAV preparations include tools for serotype testing, optogenetics, chemogenetics, biosensors, retrograde AAV, and more. Addgene's COVID-19 collection includes plasmids expressing SARS-CoV-2 open reading frames, ACE2, TMPRSS2, FURIN, and more. Addgene's repository contains over 90,000 plasmids.ADDGENE: PINDUCER20
Plasmid pInducer20 from Dr. Stephen Elledge's lab is published in Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 10.1073/pnas.1019736108. Epub 2011 Feb 9.TLCV2 - ADDGENE
Plasmid TLCV2 from Dr. Adam Karpf's lab contains the insert Cas9-2A-eGFP and is published in Cancers (Basel). 2019 Feb 21;11(2). pii: cancers11020251. doi: 10.3390/cancers11020251. This plasmid is available through Addgene.ADDGENE: PTF101.1
Plasmid pTF101.1 from Dr. Kan Wang's lab is published in Euphytica (2004) 136: 167. This plasmid is available through Addgene. ADDGENE: PINDUCER10-MIR-RUP-PHES Plasmid pInducer10-mir-RUP-PheS from Dr. Stephen Elledge's lab contains the insert PheS Gly294 and is published in Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 10.1073/pnas.1019736108. Epub 2011 Feb 9. This plasmid is available through Addgene.PX551 - ADDGENE
Plasmid PX551 from Dr. Feng Zhang's lab contains the insert SpCas9 and is published in Nat Biotechnol. 2014 Oct 19. doi: 10.1038/nbt.3055. This plasmid is available through Addgene.ADDGENE: PRGEB32
Plasmid pRGEB32 from Dr. Yinong Yang's lab is published in Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3570-5. doi: 10.1073/pnas.1420294112. Epub 2015 Mar 2.ADDGENE: PPD95_75
Image: Illustrated plasmid map in PNG format GenBank File: Plasmid sequence and annotations. Use text editor or plasmid mapping software to view sequence. SnapGene File: Plasmid sequence and SnapGene enhanced annotations. Use with SnapGene software or the free Viewer to visualize additional data and align other sequences.ADDGENE: PRGEB31
Plasmid pRGEB31 from Dr. Yinong Yang's lab is published in Mol Plant. 2013 Nov;6(6):1975-83. doi: 10.1093/mp/sst119. Epub 2013 Aug 17. This plasmid is available through Addgene. ADDGENE: PSPCAS9(BB)-2A-GFP (PX458) Plasmid pSpCas9(BB)-2A-GFP (PX458) from Dr. Feng Zhang's lab contains the insert hSpCas9 and is published in Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. This plasmid is available through Addgene.ADDGENE
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.TLCV2 - ADDGENE
Plasmid TLCV2 from Dr. Adam Karpf's lab contains the insert Cas9-2A-eGFP and is published in Cancers (Basel). 2019 Feb 21;11(2). pii: cancers11020251. doi: 10.3390/cancers11020251. This plasmid is available through Addgene. ADDGENE: HUMAN BETA-CATENIN PCDNA3 Plasmid human beta-catenin pcDNA3 from Dr. Eric Fearon's lab contains the insert beta-catenin and is published in Mol Cell Biol. 1999 Aug . 19(8):5696-706. This plasmid is available through Addgene. ADDGENE: PCAP03-ACC(3)IV This method allows the use of shorter capture arms and conveniently selects against non-homologous end joining in the presence of 5-fluoroorotic acid (5-FOA). In order to easy linearize pCAP03, the 1369 bp apramycin resistance cassette (acc (3)IV) was amplified from pIJ773 and cloned into the pCAP03 to obtain pCAP03-acc (3)IV . Vectortype.
ADDGENE: PCOMB3XSS
Plasmid pComb3XSS from Dr. Carlos Barbas's lab contains the inserts SS Stuffer, Light Chain Stuffer, and Heavy Chain Stuffer and is published in J Immunol Methods. 2000 Aug 28;242(1-2):159-81. This plasmid is available through Addgene.ADDGENE
cells, resulting in a more homogenous (but still polyclonal) cell population.Depending on the transducibilityregularly to confirm transgene expression. As polyclonal populations of resistant cells start coming through *Pro-Tip* This selection method results in a polyclonal cell population, meaning that the transgene hasfrom different cells in the population. ADDGENE: PSPCAS9(BB)-2A-GFP (PX458) Plasmid pSpCas9(BB)-2A-GFP (PX458) from Dr. Feng Zhang's lab contains the insert hSpCas9 and is published in Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. This plasmid is available through Addgene. ADDGENE: PLVX-M-PURO Plasmid pLVX-M-puro from Dr. Boyi Gan's lab is published in Nat Cell Biol. 2018 Oct;20(10):1181-1192. doi: 10.1038/s41556-018-0178-0. Epub 2018 Sep 10. This plasmid is available through Addgene. ADDGENE: PLVET-IRES-GFP Plasmid pLVET-IRES-GFP from Dr. Aki Manninen's lab is published in Oncogene. 2017 Oct 12;36(41):5681-5694. doi: 10.1038/onc.2017.177.Epub 2017 Jun
ADDGENE: PSPCAS9(BB)-2A-PURO (PX459) V2.0 Plasmid pSpCas9(BB)-2A-Puro (PX459) V2.0 from Dr. Feng Zhang's lab contains the insert hSpCas9-2A-Puro V2.0 and is published in Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. This plasmid is available through Addgene. Skip to main content This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies. Close Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learnmore
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Addgene's CRISPR collection includes plasmids for gene disruption, RNA base editing, pooled library screening, and more. Browse CRISPRCollection
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Addgene's ready-to-use AAV preparations include tools for serotype testing, optogenetics, chemogenetics, biosensors, retrograde AAV, andmore. Browse AAV
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Addgene's COVID-19 collection includes plasmids expressing SARS-CoV-2 open reading frames, ACE2, TMPRSS2, FURIN, and more. Browse CollectionAll Collections
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Plasmid of the Day
pHH0103_STAC3-2/2
DEPOSITOR: Sachdev Sidhu PURPOSE: Protein expression and purification of human SH3 domainconstruct STAC3-2/2
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pAAV.CaMKIIa.ChETA(E123T/H1… DEPOSITOR: Karl Deisseroth PURPOSE: Ready-to-use AAV5 particles produced from pAAV.CaMKIIa.ChETA(E123T/H134R)-eYFP.WPRE.hGH (#100050). CaMKIIa-driven, ChETA(E123T/H134R)-eYFP expression for optogenetic activation. These AAV preparations are suitable purity for injectioninto animals.
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Our optogenetic tools are sweeping throughout neuroscience, helping scientists figure out how the brain works. Without Addgene helping provide these to the world, neuroscience would be moving at a muchslower pace.
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