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BIOANALYSIS
Bioanalysis welcomes unsolicited article proposals. Email us today to discuss the suitability of your research and our options for authors, including our Accelerated Publication and Open Access services. Visit Bioanalysis Zone our online community. Supplement: The Bioanalysis Glossary. View the Decennial Index of the WRIB White Papers. TUMOR CELL INVASION INTO MATRIGEL: OPTIMIZED PROTOCOL FOR An optimized method for RNA extraction from highly invasive glioma cells embedded into Matrigel was achieved by using TRIzol, used for matrix dissociation, cell lysis and RNA separation, in combination with a specific commercial kit used to perform the DNAse treatment and optimal RNA purification on column. A STRATEGIC APPROACH TO NONCLINICAL IMMUNOGENICITY Review of the current recommendations. Guidance for nonclinical immunogenicity assessments is sparse. However, the key points from published literature and guidelines are summarized in Table 1.Initial requirements for assays used for the detection of host antibodies against biotechnology products were published to provide harmonized scientific recommendations for assays used in RATIONAL APPLICATION OF DRUG PROMISCUITY IN MEDICINAL Rational application of drug promiscuity in medicinal chemistry. ‘Drug promiscuity’ refers to a drug that can act on multiple molecular targets, exhibiting similar or different pharmacological effects. Drugs may interact with unwanted targets, leading to off-target effects (one of the main reasons for side effects). Thus,intervention to
CRISPR-CAS13A-BASED DIAGNOSTIC METHOD FOR CHLAMYDIA Aim: Development of a routine screening technique for Chlamydia trachomatis infection. The proposed approach involves the CRISPR RNA (crRNA). In the presence of the target sequence, the RNase activity of the Cas13a protein is activated, and it cleaves RNA fluorescent probe so that fluorescence will be emitted. THE NEW FACE OF ELECTROPHORESIS: MODERNIZATION OF A Automated Analysis of Protein Purity. One of the most appropriate applications of automated protein electrophoresis is to accelerate process development by monitoring column fractions during chromatographic purification ().This can apply to checking the purity of a monoclonal antibody in an industrial quality control setting or following the purification of an engineered polyhistidinetagged INTELLECTUAL PROPERTY AND ITS ROLE IN THE PHARMACEUTICAL Intellectual property & its role in the pharmaceutical industry | Editorial future science group www.future-science.com 1549 companies tend to obtain patent protection in DRUG DELIVERY TO THE LUNGS: CHALLENGES AND OPPORTUNITIES 648 Ther. Deliv. (2017) 8(8) Review Newman mechanisms that are intended to keep inhaled m aterials out of the lungs, as well as removing or inactivating them once they have been deposited.Second,it is
EBF RECOMMENDATION FOR STABILITY TESTING OF ANTI-DRUG future science rou www.future-science.com 1411 EBF recommendation for stability testing of anti-drug antibodies White Paper on the methodological approaches (e.g., RECOMMENDATIONS FOR THE DEVELOPMENT AND VALIDATION OF 1620 Bioanalysis (2015) 7(13) White Paper Jani, Marsden, Mikulskis et al. minimum of 50% inhibition, to widely accepted and applied complex statistical methods using the variation of percent inhibitions from a therapeutic-naïve popu-BIOANALYSIS
Bioanalysis welcomes unsolicited article proposals. Email us today to discuss the suitability of your research and our options for authors, including our Accelerated Publication and Open Access services. Visit Bioanalysis Zone our online community. Supplement: The Bioanalysis Glossary. View the Decennial Index of the WRIB White Papers. TUMOR CELL INVASION INTO MATRIGEL: OPTIMIZED PROTOCOL FOR An optimized method for RNA extraction from highly invasive glioma cells embedded into Matrigel was achieved by using TRIzol, used for matrix dissociation, cell lysis and RNA separation, in combination with a specific commercial kit used to perform the DNAse treatment and optimal RNA purification on column. A STRATEGIC APPROACH TO NONCLINICAL IMMUNOGENICITY Review of the current recommendations. Guidance for nonclinical immunogenicity assessments is sparse. However, the key points from published literature and guidelines are summarized in Table 1.Initial requirements for assays used for the detection of host antibodies against biotechnology products were published to provide harmonized scientific recommendations for assays used in RATIONAL APPLICATION OF DRUG PROMISCUITY IN MEDICINAL Rational application of drug promiscuity in medicinal chemistry. ‘Drug promiscuity’ refers to a drug that can act on multiple molecular targets, exhibiting similar or different pharmacological effects. Drugs may interact with unwanted targets, leading to off-target effects (one of the main reasons for side effects). Thus,intervention to
CRISPR-CAS13A-BASED DIAGNOSTIC METHOD FOR CHLAMYDIA Aim: Development of a routine screening technique for Chlamydia trachomatis infection. The proposed approach involves the CRISPR RNA (crRNA). In the presence of the target sequence, the RNase activity of the Cas13a protein is activated, and it cleaves RNA fluorescent probe so that fluorescence will be emitted. THE NEW FACE OF ELECTROPHORESIS: MODERNIZATION OF A Automated Analysis of Protein Purity. One of the most appropriate applications of automated protein electrophoresis is to accelerate process development by monitoring column fractions during chromatographic purification ().This can apply to checking the purity of a monoclonal antibody in an industrial quality control setting or following the purification of an engineered polyhistidinetagged INTELLECTUAL PROPERTY AND ITS ROLE IN THE PHARMACEUTICAL Intellectual property & its role in the pharmaceutical industry | Editorial future science group www.future-science.com 1549 companies tend to obtain patent protection in DRUG DELIVERY TO THE LUNGS: CHALLENGES AND OPPORTUNITIES 648 Ther. Deliv. (2017) 8(8) Review Newman mechanisms that are intended to keep inhaled m aterials out of the lungs, as well as removing or inactivating them once they have been deposited.Second,it is
EBF RECOMMENDATION FOR STABILITY TESTING OF ANTI-DRUG future science rou www.future-science.com 1411 EBF recommendation for stability testing of anti-drug antibodies White Paper on the methodological approaches (e.g., RECOMMENDATIONS FOR THE DEVELOPMENT AND VALIDATION OF 1620 Bioanalysis (2015) 7(13) White Paper Jani, Marsden, Mikulskis et al. minimum of 50% inhibition, to widely accepted and applied complex statistical methods using the variation of percent inhibitions from a therapeutic-naïve popu-BIOANALYSIS
Bioanalysis welcomes unsolicited article proposals. Email us today to discuss the suitability of your research and our options for authors, including our Accelerated Publication and Open Access services. Visit Bioanalysis Zone our online community. Supplement: The Bioanalysis Glossary. View the Decennial Index of the WRIB White Papers.FUTURE SCIENCE OA
Future Science OA welcomes unsolicited article proposals. Email us today to discuss the suitability of your research and our options for authors, including our Accelerated Publication service, ensuring rapid publication of your article subject to acceptance. Not sure why you should publish open access? A STRATEGIC APPROACH TO NONCLINICAL IMMUNOGENICITY Review of the current recommendations. Guidance for nonclinical immunogenicity assessments is sparse. However, the key points from published literature and guidelines are summarized in Table 1.Initial requirements for assays used for the detection of host antibodies against biotechnology products were published to provide harmonized scientific recommendations for assays used in 2020WHITEPAPERONRECENTISSUESIN BIOANALYSIS WhitePaper For reprint orders, please contact: reprints@future-science.com 2020WhitePaperonRecentIssuesin Bioanalysis:VaccineAssayValidation,qPCR A NOVEL METHOD FOR REMOVING POLYETHYLENEIMINE FROM Detecting and quantifying residual host cell DNA is an essential impurity test for all biopharmaceutical products; levels of residual DNA must be closely monitored and controlled throughout the production and release of the drug substance. qPCR is a widely accepted approach for the quantification of residual DNA in recombinant therapeutic proteins and the TaqMan qPCR ASSAYS AND TECHNOLOGIES FOR DEVELOPING PROTEOLYSIS Review Liu,Zhang,Lv,Yuan,Zheng&Zhou Degradation pathway Ub Ub Ub Ub Ub Ub E2 E2 E3 Ub E2 E3 E3 Binary target engagement Ub Ub Ub Ub Ub Ub Ub Ub Recycle Proteasome PROTACs Penetration Ternary complex formation ANALYSIS AND QUANTIFICATION OF IN VITRO MYOBLAST FUSION Adult skeletal muscle tissue contains a population of precursor cells, known as satellite cells, that are responsible for skeletal muscle repair (1–3).Activated satellite cells (myoblasts) migrate to the site of muscle trauma, where they proliferate, differentiate, and fuse into multinucleated myotubes (3–5).The ultimate success of this process is measured by the degree to which resident DRUG DELIVERY TO THE LUNGS: CHALLENGES AND OPPORTUNITIES 648 Ther. Deliv. (2017) 8(8) Review Newman mechanisms that are intended to keep inhaled m aterials out of the lungs, as well as removing or inactivating them once they have been deposited.Second,it is
BIOANALYSIS AUTHOR GUIDELINES Bioanalysis author guidelines Future perspective - The author is challenged to include speculative viewpoint on how the field will have evolved 5–10 years from the point at which the article was written. Executive summary - A series of bulleted statements representing key conclusions, unresolved issues and points for emphasis of work in future, under the main headings of BIOANALYSIS FOR PLASMA PROTEIN BINDING STUDIES IN DRUG Drug molecules in animals and humans are either bound to proteins and lipids or are freely available. This article focuses on analysis ofprotein bind-
BIOANALYSIS
Bioanalysis welcomes unsolicited article proposals. Email us today to discuss the suitability of your research and our options for authors, including our Accelerated Publication and Open Access services. Visit Bioanalysis Zone our online community. Supplement: The Bioanalysis Glossary. View the Decennial Index of the WRIB White Papers. TUMOR CELL INVASION INTO MATRIGEL: OPTIMIZED PROTOCOL FOR An optimized method for RNA extraction from highly invasive glioma cells embedded into Matrigel was achieved by using TRIzol, used for matrix dissociation, cell lysis and RNA separation, in combination with a specific commercial kit used to perform the DNAse treatment and optimal RNA purification on column. A STRATEGIC APPROACH TO NONCLINICAL IMMUNOGENICITY Review of the current recommendations. Guidance for nonclinical immunogenicity assessments is sparse. However, the key points from published literature and guidelines are summarized in Table 1.Initial requirements for assays used for the detection of host antibodies against biotechnology products were published to provide harmonized scientific recommendations for assays used in RATIONAL APPLICATION OF DRUG PROMISCUITY IN MEDICINAL Rational application of drug promiscuity in medicinal chemistry. ‘Drug promiscuity’ refers to a drug that can act on multiple molecular targets, exhibiting similar or different pharmacological effects. Drugs may interact with unwanted targets, leading to off-target effects (one of the main reasons for side effects). Thus,intervention to
CRISPR-CAS13A-BASED DIAGNOSTIC METHOD FOR CHLAMYDIA Aim: Development of a routine screening technique for Chlamydia trachomatis infection. The proposed approach involves the CRISPR RNA (crRNA). In the presence of the target sequence, the RNase activity of the Cas13a protein is activated, and it cleaves RNA fluorescent probe so that fluorescence will be emitted. THE NEW FACE OF ELECTROPHORESIS: MODERNIZATION OF A Automated Analysis of Protein Purity. One of the most appropriate applications of automated protein electrophoresis is to accelerate process development by monitoring column fractions during chromatographic purification ().This can apply to checking the purity of a monoclonal antibody in an industrial quality control setting or following the purification of an engineered polyhistidinetagged INTELLECTUAL PROPERTY AND ITS ROLE IN THE PHARMACEUTICAL Intellectual property & its role in the pharmaceutical industry | Editorial future science group www.future-science.com 1549 companies tend to obtain patent protection in DRUG DELIVERY TO THE LUNGS: CHALLENGES AND OPPORTUNITIES 648 Ther. Deliv. (2017) 8(8) Review Newman mechanisms that are intended to keep inhaled m aterials out of the lungs, as well as removing or inactivating them once they have been deposited.Second,it is
EBF RECOMMENDATION FOR STABILITY TESTING OF ANTI-DRUG future science rou www.future-science.com 1411 EBF recommendation for stability testing of anti-drug antibodies White Paper on the methodological approaches (e.g., RECOMMENDATIONS FOR THE DEVELOPMENT AND VALIDATION OF 1620 Bioanalysis (2015) 7(13) White Paper Jani, Marsden, Mikulskis et al. minimum of 50% inhibition, to widely accepted and applied complex statistical methods using the variation of percent inhibitions from a therapeutic-naïve popu-BIOANALYSIS
Bioanalysis welcomes unsolicited article proposals. Email us today to discuss the suitability of your research and our options for authors, including our Accelerated Publication and Open Access services. Visit Bioanalysis Zone our online community. Supplement: The Bioanalysis Glossary. View the Decennial Index of the WRIB White Papers. TUMOR CELL INVASION INTO MATRIGEL: OPTIMIZED PROTOCOL FOR An optimized method for RNA extraction from highly invasive glioma cells embedded into Matrigel was achieved by using TRIzol, used for matrix dissociation, cell lysis and RNA separation, in combination with a specific commercial kit used to perform the DNAse treatment and optimal RNA purification on column. A STRATEGIC APPROACH TO NONCLINICAL IMMUNOGENICITY Review of the current recommendations. Guidance for nonclinical immunogenicity assessments is sparse. However, the key points from published literature and guidelines are summarized in Table 1.Initial requirements for assays used for the detection of host antibodies against biotechnology products were published to provide harmonized scientific recommendations for assays used in RATIONAL APPLICATION OF DRUG PROMISCUITY IN MEDICINAL Rational application of drug promiscuity in medicinal chemistry. ‘Drug promiscuity’ refers to a drug that can act on multiple molecular targets, exhibiting similar or different pharmacological effects. Drugs may interact with unwanted targets, leading to off-target effects (one of the main reasons for side effects). Thus,intervention to
CRISPR-CAS13A-BASED DIAGNOSTIC METHOD FOR CHLAMYDIA Aim: Development of a routine screening technique for Chlamydia trachomatis infection. The proposed approach involves the CRISPR RNA (crRNA). In the presence of the target sequence, the RNase activity of the Cas13a protein is activated, and it cleaves RNA fluorescent probe so that fluorescence will be emitted. THE NEW FACE OF ELECTROPHORESIS: MODERNIZATION OF A Automated Analysis of Protein Purity. One of the most appropriate applications of automated protein electrophoresis is to accelerate process development by monitoring column fractions during chromatographic purification ().This can apply to checking the purity of a monoclonal antibody in an industrial quality control setting or following the purification of an engineered polyhistidinetagged INTELLECTUAL PROPERTY AND ITS ROLE IN THE PHARMACEUTICAL Intellectual property & its role in the pharmaceutical industry | Editorial future science group www.future-science.com 1549 companies tend to obtain patent protection in DRUG DELIVERY TO THE LUNGS: CHALLENGES AND OPPORTUNITIES 648 Ther. Deliv. (2017) 8(8) Review Newman mechanisms that are intended to keep inhaled m aterials out of the lungs, as well as removing or inactivating them once they have been deposited.Second,it is
EBF RECOMMENDATION FOR STABILITY TESTING OF ANTI-DRUG future science rou www.future-science.com 1411 EBF recommendation for stability testing of anti-drug antibodies White Paper on the methodological approaches (e.g., RECOMMENDATIONS FOR THE DEVELOPMENT AND VALIDATION OF 1620 Bioanalysis (2015) 7(13) White Paper Jani, Marsden, Mikulskis et al. minimum of 50% inhibition, to widely accepted and applied complex statistical methods using the variation of percent inhibitions from a therapeutic-naïve popu-BIOANALYSIS
Bioanalysis welcomes unsolicited article proposals. Email us today to discuss the suitability of your research and our options for authors, including our Accelerated Publication and Open Access services. Visit Bioanalysis Zone our online community. Supplement: The Bioanalysis Glossary. View the Decennial Index of the WRIB White Papers.FUTURE SCIENCE OA
Future Science OA welcomes unsolicited article proposals. Email us today to discuss the suitability of your research and our options for authors, including our Accelerated Publication service, ensuring rapid publication of your article subject to acceptance. Not sure why you should publish open access? A STRATEGIC APPROACH TO NONCLINICAL IMMUNOGENICITY Review of the current recommendations. Guidance for nonclinical immunogenicity assessments is sparse. However, the key points from published literature and guidelines are summarized in Table 1.Initial requirements for assays used for the detection of host antibodies against biotechnology products were published to provide harmonized scientific recommendations for assays used in 2020WHITEPAPERONRECENTISSUESIN BIOANALYSIS WhitePaper For reprint orders, please contact: reprints@future-science.com 2020WhitePaperonRecentIssuesin Bioanalysis:VaccineAssayValidation,qPCR A NOVEL METHOD FOR REMOVING POLYETHYLENEIMINE FROM Detecting and quantifying residual host cell DNA is an essential impurity test for all biopharmaceutical products; levels of residual DNA must be closely monitored and controlled throughout the production and release of the drug substance. qPCR is a widely accepted approach for the quantification of residual DNA in recombinant therapeutic proteins and the TaqMan qPCR ASSAYS AND TECHNOLOGIES FOR DEVELOPING PROTEOLYSIS Review Liu,Zhang,Lv,Yuan,Zheng&Zhou Degradation pathway Ub Ub Ub Ub Ub Ub E2 E2 E3 Ub E2 E3 E3 Binary target engagement Ub Ub Ub Ub Ub Ub Ub Ub Recycle Proteasome PROTACs Penetration Ternary complex formation ANALYSIS AND QUANTIFICATION OF IN VITRO MYOBLAST FUSION Adult skeletal muscle tissue contains a population of precursor cells, known as satellite cells, that are responsible for skeletal muscle repair (1–3).Activated satellite cells (myoblasts) migrate to the site of muscle trauma, where they proliferate, differentiate, and fuse into multinucleated myotubes (3–5).The ultimate success of this process is measured by the degree to which resident DRUG DELIVERY TO THE LUNGS: CHALLENGES AND OPPORTUNITIES 648 Ther. Deliv. (2017) 8(8) Review Newman mechanisms that are intended to keep inhaled m aterials out of the lungs, as well as removing or inactivating them once they have been deposited.Second,it is
BIOANALYSIS AUTHOR GUIDELINES Bioanalysis author guidelines Future perspective - The author is challenged to include speculative viewpoint on how the field will have evolved 5–10 years from the point at which the article was written. Executive summary - A series of bulleted statements representing key conclusions, unresolved issues and points for emphasis of work in future, under the main headings of BIOANALYSIS FOR PLASMA PROTEIN BINDING STUDIES IN DRUG Drug molecules in animals and humans are either bound to proteins and lipids or are freely available. This article focuses on analysis ofprotein bind-
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If the address matches an existing account you will receive an email with instructions to reset your password. THERAPEUTIC DELIVERY If the address matches an existing account you will receive an email with instructions to reset your password. FUTURE MEDICINAL CHEMISTRY If the address matches an existing account you will receive an email with instructions to reset your password. THE NEW FACE OF ELECTROPHORESIS: MODERNIZATION OF A Automated Analysis of Protein Purity. One of the most appropriate applications of automated protein electrophoresis is to accelerate process development by monitoring column fractions during chromatographic purification ().This can apply to checking the purity of a monoclonal antibody in an industrial quality control setting or following the purification of an engineered polyhistidinetagged RATIONAL APPLICATION OF DRUG PROMISCUITY IN MEDICINAL ‘Drug promiscuity’ refers to a drug that can act on multiple molecular targets, exhibiting similar or different pharmacological effects. Drugs may interact with unwanted targets, leading to off-target effects (one of the main reasons for side effects).FUTURE SCIENCE OA
If the address matches an existing account you will receive an email with instructions to reset your password.BIOANALYSIS
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If the address matches an existing account you will receive an email with instructions to reset your password. FUTURE MEDICINAL CHEMISTRY If the address matches an existing account you will receive an email with instructions to reset your password.FUTURE SCIENCE OA
If the address matches an existing account you will receive an email with instructions to reset your password. THERAPEUTIC DELIVERY If the address matches an existing account you will receive an email with instructions to reset your password. FUTURE MEDICINAL CHEMISTRY If the address matches an existing account you will receive an email with instructions to reset your password. THE NEW FACE OF ELECTROPHORESIS: MODERNIZATION OF A Automated Analysis of Protein Purity. One of the most appropriate applications of automated protein electrophoresis is to accelerate process development by monitoring column fractions during chromatographic purification ().This can apply to checking the purity of a monoclonal antibody in an industrial quality control setting or following the purification of an engineered polyhistidinetagged RATIONAL APPLICATION OF DRUG PROMISCUITY IN MEDICINAL ‘Drug promiscuity’ refers to a drug that can act on multiple molecular targets, exhibiting similar or different pharmacological effects. Drugs may interact with unwanted targets, leading to off-target effects (one of the main reasons for side effects).FUTURE SCIENCE
If the address matches an existing account you will receive an email with instructions to reset your password.BIOANALYSIS
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If the address matches an existing account you will receive an email with instructions to reset your password. THERAPEUTIC DELIVERY If the address matches an existing account you will receive an email with instructions to reset your password. PRECISE GENE DELETION AND REPLACEMENT USING THE CRISPR The prokaryotic type II CRISPR/Cas9 system has been adapted to perform targeted genome editing in cells and model organisms. Here, we describe targeted gene deletion and replacement in human cells via the CRISPR/Cas9 system using two guide RNAs. A PRACTICAL GUIDE TO FILTERING AND PRIORITIZING GENETIC Next-generation sequencing (NGS) of whole genomes and exomes is a powerful tool in biomedical research and clinical diagnostics. However, the vast amount of data produced by NGS introduces new challenges and opportunities, many of which require novel computational and theoretical approaches when it comes to identifying the causal variant(s) for a disease of interest. QPCR: AVOIDING SIGNALS IN THE NO-TEMPLATE CONTROL Molecular Biology Techniques Q&A. How can I avoid technical replicate variation and signals in my no-template controls? (Thread 21619) Q I am having some problems with qPCR using plant RNA. I see amplification in the no-template control (NTC) reactions and unacceptable technical replicate differences in the Ct values. BIOANALYSIS FOR PLASMA PROTEIN BINDING STUDIES IN DRUG Drug molecules in animals and humans are either bound to proteins and lipids or are freely available. This article focuses on analysis ofprotein bind-
PROTOCOL FOR ADHESION AND IMMUNOSTAINING OF LYMPHOCYTES Immunostaining of non-adherent cells is commonly performed after adhesion of cells onto microscope slides either using cytocentrifugation or with the RECOMMENDATIONS FOR THE DEVELOPMENT AND VALIDATION OF 1620 Bioanalysis (2015) 7(13) White Paper Jani, Marsden, Mikulskis et al. minimum of 50% inhibition, to widely accepted and applied complex statistical methods using the variation of percent inhibitions from a therapeutic-naïve popu- We use cookies to improve your experience. By continuing to browse this site, you accept our cookie policy .×__
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