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lab bench.
ENTRY LEVEL OCTET SYSTEM Octet K2 System. The Octet® K2 2-channel System brings you unprecedented access to exquisite biomolecular interactions data. Priced for startup academic and biotech labs, low volume users are no longer bound by the trade-off between cost and performance when choosing a label-free assay system.SAFETY DATA SHEET
Conforms to Regulation (EC) No. 1907/2006 (REACH), Annex II, as amended by Commission Regulation (EU) 2015/830 - United Kingdom (UK) 1.3 Details of the supplier of the safety data sheet PROTEIN L BIOSENSORS Protein L Biosensors For whole molecule antibody and antibody fragment quantitation Key features • Broader recognition of Ig isotypes and species than Protein A or G • Regenerable and cost-effective format • Binds whole molecule Ab and FAb fragments • Quantitate in crude matrices Overview Dip and Read™ Protein L (ProL) biosensors providea rapid and
OCTET HTX SYSTEM
Octet HTX System Accelerate your label-free workflow Key features • 96-well plate quantitation in 2 minutes • 32 x 32 epitope binning in less than 8 hours • Full plate off-rate ranking in minutes, not hours • trix that can accommodate a variety of cross-blocking formats Simultaneously reads 8, OPTIMIZING PROTEIN-PROTEIN AND PROTEIN-SMALL MOLECULE 2 Time (sec) Binding (nm) 0 1.2 2.4 3.6 4.8 0 600 1,200 1,800 2,400 3,000 3,600 4,200 Figure 1: Identifying which of eight solutions is best for regeneration in less than two hours.NI-NTA BIOSENSORS
Ni-NTA Biosensors For quantitation and kinetic characterization of HIS-tagged biomolecules Key features • Direct and rapid quantitation of HIS-tagged biomolecules • Easy capture of HIS-fusion proteins for kinetic analysis • Designed for use in buffer and diluted complex media • Compatible with Octet ® and BLItz® platforms Overview The penta- or six-histidine peptide tag (HIS) is CELL LINE DEVELOPMENT: ANTIBODY DISCOVERY BY TITER Key features • BLI measures only what’s captured on biosensor chemistries, Significantly reduce time to develop antibodies by combining titer and glycan screening • Save FTE costs and perform more projects using the high-throughput Octet® system Cell line development typically includes the screening of thou - FAST QUANTITATION OF PROTEINS AND ANTIBODIES USING BLITZ 2 multiple wash steps and excessive hands-on time (Figure 1). Label-free detection with the BLItz system provides a simple, fast and accurate solution for characterization and monitoring FORTEBIO ONLINE STORE Choosing a selection results in a full page refresh. Press the space key then arrow keys to make a selection. PERSONAL ASSAY SYSTEM Drop. Read. Done. The BLItz® System brings label-free analysis to individual researchers, and is affordably priced so everyone can have one. With this personal assay system, you can run protein quantitation and kinetics experiments in only 4 µL of sample—right at your ownlab bench.
ENTRY LEVEL OCTET SYSTEM Octet K2 System. The Octet® K2 2-channel System brings you unprecedented access to exquisite biomolecular interactions data. Priced for startup academic and biotech labs, low volume users are no longer bound by the trade-off between cost and performance when choosing a label-free assay system.SAFETY DATA SHEET
Conforms to Regulation (EC) No. 1907/2006 (REACH), Annex II, as amended by Commission Regulation (EU) 2015/830 - United Kingdom (UK) 1.3 Details of the supplier of the safety data sheet PROTEIN L BIOSENSORS Protein L Biosensors For whole molecule antibody and antibody fragment quantitation Key features • Broader recognition of Ig isotypes and species than Protein A or G • Regenerable and cost-effective format • Binds whole molecule Ab and FAb fragments • Quantitate in crude matrices Overview Dip and Read™ Protein L (ProL) biosensors providea rapid and
OCTET HTX SYSTEM
Octet HTX System Accelerate your label-free workflow Key features • 96-well plate quantitation in 2 minutes • 32 x 32 epitope binning in less than 8 hours • Full plate off-rate ranking in minutes, not hours • trix that can accommodate a variety of cross-blocking formats Simultaneously reads 8, OPTIMIZING PROTEIN-PROTEIN AND PROTEIN-SMALL MOLECULE 2 Time (sec) Binding (nm) 0 1.2 2.4 3.6 4.8 0 600 1,200 1,800 2,400 3,000 3,600 4,200 Figure 1: Identifying which of eight solutions is best for regeneration in less than two hours.NI-NTA BIOSENSORS
Ni-NTA Biosensors For quantitation and kinetic characterization of HIS-tagged biomolecules Key features • Direct and rapid quantitation of HIS-tagged biomolecules • Easy capture of HIS-fusion proteins for kinetic analysis • Designed for use in buffer and diluted complex media • Compatible with Octet ® and BLItz® platforms Overview The penta- or six-histidine peptide tag (HIS) is CELL LINE DEVELOPMENT: ANTIBODY DISCOVERY BY TITER Key features • BLI measures only what’s captured on biosensor chemistries, Significantly reduce time to develop antibodies by combining titer and glycan screening • Save FTE costs and perform more projects using the high-throughput Octet® system Cell line development typically includes the screening of thou - FAST QUANTITATION OF PROTEINS AND ANTIBODIES USING BLITZ 2 multiple wash steps and excessive hands-on time (Figure 1). Label-free detection with the BLItz system provides a simple, fast and accurate solution for characterization and monitoring PERSONAL ASSAY SYSTEM Drop. Read. Done. The BLItz® System brings label-free analysis to individual researchers, and is affordably priced so everyone can have one. With this personal assay system, you can run protein quantitation and kinetics experiments in only 4 µL of sample—right at your ownlab bench.
8-CHANNEL OCTET SYSTEMS, OCTET RED96E & OCTET QKE The versatile 8-channel Octet systems provide flexibility for rapid assay development. The 8-channel Octet systems perform quantitation of96 samples in 32
PROTEIN A BIOSENSORS Key features • Direct measurement of immunoglobulins (IgG) • Assay samples without centrifugation • Broad dynamic range • Fast turnaround of results • Correlates to HPLC Quick facts • Dynamic range: 1–500 μg/mL for most proteins • Throughput: 8 samples in ~ 2 minutes, 96 samples in ~ 32 minutes • Precision/accuracy: < 10% CVs • Limit of detection: typically 1 μg/mL OCTET KINETICS ASSAY: METHOD DEVELOPMENT GUIDELINE 1 1. Purpose 1.1 The purpose of this document is to describe the procedure for developing a ligand binding kinetics assay on Octet® QKe, RED96, RED96e, RED384 and HTX instruments. MAB QUANTITATION: PROTEIN A HPLC VS. PROTEIN A BIO-LAYER 3 curves were then treated as experimental points, and tested for their fit to the average standard curve based on their expected value. Percent Bias (%Bias) could then be calculated over the ANTI-HUMAN IGG (AHQ) BIOSENSORS (FC-SPECIFIC) Key features • Direct measurement of immunoglobulins (IgG) • Assay samples without centrifugation • Fast turnaround of results • Correlates to HPLC ForteBio’s Dip and Read™ Anti-Human IgG (AHQ) biosensors, in conjunction with the Octet® platform, are designed for mon- itoring antibody concentrations from crude lysates and cellSAFETY DATA SHEET
Conforms to Regulation (EC) No. 1907/2006 (REACH), Annex II, as amended by Commission Regulation (EU) 2015/830 - United Kingdom (UK) 1.3 Details of the supplier of the safety data sheet OCTET SYSTEM QUALIFICATION PRODUCTS AND SERVICES Title: Octet System Qualification Products and Services | Molecular Devices Author: Fortebio Subject: Read this document which describes the Octet system qualification products and services for label-free, real-time detection of biomolecular interactions, applications, andservices.
ANTI-HUMAN FAB-CH1 2ND GENERATION BIOSENSORS Key features • Rapid quantitation of all subclasses of human Fab, F(ab′) 2, and IgG • Easy capture of human Fab, F(ab′) 2, and IgG for kinetic analysis with antigen • Capture of IgG for analysis of Fc receptor binding • Biosensor greatly accelerates laboratory workflows and reducNo recognition towards human kappa or lambda light chains • No cross-reactivity towards bovine or OCTET SOFTWARE VERSION 10: DATA ACQUISITION AND DATA Octet Data Analysis software version feature comparison Feature V8.x V9.x V10.x Quantitation Mask all proprietary information (sample ID, sensor info, etc.) from data ü ü Editable cells, customize and sort Results table ü ü Import standard curve of experiments run at thesame temperature
FORTEBIO ONLINE STORE Chemical & Consumer Care. Pulp and Paper. Vaccines. SARS-CoV-2 (COVID-19) Vaccine research, testing, and production solutions. From Cell Line Development to Lot Release. Explore our portfolio that supports your drug development process. Products. Protein Analysis. Biosensors, Sensor Chips & Kits. 8-CHANNEL OCTET SYSTEMS, OCTET RED96E & OCTET QKE The versatile 8-channel Octet systems provide flexibility for rapid assay development. The 8-channel Octet systems perform quantitation of 96 samples in 32 minutes and kinetic screening of 64 samples in 1.5 hours. Analysis can be done using a single channel or up to eight channels, enabling flexibility in sample throughput based on need. ENTRY LEVEL OCTET SYSTEM The Octet® K2 2-channel System brings you unprecedented access to exquisite biomolecular interactions data. Priced for startup academic and biotech labs, low volume users are no longer bound by the trade-off between cost and performance when choosing a label-free assay system. The Octet K2 System characterizes protein-protein andprotein-small
PERSONAL ASSAY SYSTEM The BLItz® system provides a simple, rapid Dip and Read™ approach for protein quantitation in a small, affordably priced personal assay system. With BLItz, proteins and antibodies can be quantitated in a matter of seconds with high specificity and sensitivity, even in crude samples. The system utilizes the same proprietary Bio-LayerSAFETY DATA SHEET
Conforms to Regulation (EC) No. 1907/2006 (REACH), Annex II, as amended by Commission Regulation (EU) 2015/830 - United Kingdom (UK) 1.3 Details of the supplier of the safety data sheetOCTET HTX SYSTEM
Octet HTX System Accelerate your label-free workflow Key features • 96-well plate quantitation in 2 minutes • 32 x 32 epitope binning in less than 8 hours • Full plate off-rate ranking in minutes, not hours • trix that can accommodate a variety of cross-blocking formats Simultaneously reads 8,OCTET QKE SYSTEM
Key features • Increased sensitivity and dynamic range for protein quantitation • Excellent signal resolution for measuring protein and peptide kinetics • Greater operational cost savings with biosensor re-racking and regeneration The Octet® QKe system is the enhanced-performance version of the first-generation Octet QK instrument from the Octet family PROTEIN L BIOSENSORS Protein L Biosensors For whole molecule antibody and antibody fragment quantitation Key features • Broader recognition of Ig isotypes and species than Protein A or G • Regenerable and cost-effective format • Binds whole molecule Ab and FAb fragments • Quantitate in crude matrices Overview Dip and Read™ Protein L (ProL) biosensors providea rapid and
OCTET POTENCY ASSAY: DEVELOPMENT, QUALIFICATION AND 3 Time (sec) Binding (nm) 0 50 100 150 200 250 300 350 0 0.5 1.0 1.5 2.0 2.5 A1 B1 C1 D1 BIOSENSOR SELECTION Ni-NTA biosensors were selected for this assay for robustness, CELL LINE DEVELOPMENT: ANTIBODY DISCOVERY BY TITER Key features • BLI measures only what’s captured on biosensor chemistries, Significantly reduce time to develop antibodies by combining titer and glycan screening • Save FTE costs and perform more projects using the high-throughput Octet® system Cell line development typically includes the screening of thou - FORTEBIO ONLINE STORE Chemical & Consumer Care. Pulp and Paper. Vaccines. SARS-CoV-2 (COVID-19) Vaccine research, testing, and production solutions. From Cell Line Development to Lot Release. Explore our portfolio that supports your drug development process. Products. Protein Analysis. Biosensors, Sensor Chips & Kits. 8-CHANNEL OCTET SYSTEMS, OCTET RED96E & OCTET QKE The versatile 8-channel Octet systems provide flexibility for rapid assay development. The 8-channel Octet systems perform quantitation of 96 samples in 32 minutes and kinetic screening of 64 samples in 1.5 hours. Analysis can be done using a single channel or up to eight channels, enabling flexibility in sample throughput based on need. PERSONAL ASSAY SYSTEM The BLItz® system provides a simple, rapid Dip and Read™ approach for protein quantitation in a small, affordably priced personal assay system. With BLItz, proteins and antibodies can be quantitated in a matter of seconds with high specificity and sensitivity, even in crude samples. The system utilizes the same proprietary Bio-Layer ENTRY LEVEL OCTET SYSTEM The Octet® K2 2-channel System brings you unprecedented access to exquisite biomolecular interactions data. Priced for startup academic and biotech labs, low volume users are no longer bound by the trade-off between cost and performance when choosing a label-free assay system. The Octet K2 System characterizes protein-protein andprotein-small
SAFETY DATA SHEET
Conforms to Regulation (EC) No. 1907/2006 (REACH), Annex II, as amended by Commission Regulation (EU) 2015/830 - United Kingdom (UK) 1.3 Details of the supplier of the safety data sheetOCTET HTX SYSTEM
Octet HTX System Accelerate your label-free workflow Key features • 96-well plate quantitation in 2 minutes • 32 x 32 epitope binning in less than 8 hours • Full plate off-rate ranking in minutes, not hours • trix that can accommodate a variety of cross-blocking formats Simultaneously reads 8,OCTET QKE SYSTEM
Key features • Increased sensitivity and dynamic range for protein quantitation • Excellent signal resolution for measuring protein and peptide kinetics • Greater operational cost savings with biosensor re-racking and regeneration The Octet® QKe system is the enhanced-performance version of the first-generation Octet QK instrument from the Octet family PROTEIN L BIOSENSORS Protein L Biosensors For whole molecule antibody and antibody fragment quantitation Key features • Broader recognition of Ig isotypes and species than Protein A or G • Regenerable and cost-effective format • Binds whole molecule Ab and FAb fragments • Quantitate in crude matrices Overview Dip and Read™ Protein L (ProL) biosensors providea rapid and
OCTET POTENCY ASSAY: DEVELOPMENT, QUALIFICATION AND 3 Time (sec) Binding (nm) 0 50 100 150 200 250 300 350 0 0.5 1.0 1.5 2.0 2.5 A1 B1 C1 D1 BIOSENSOR SELECTION Ni-NTA biosensors were selected for this assay for robustness, CELL LINE DEVELOPMENT: ANTIBODY DISCOVERY BY TITER Key features • BLI measures only what’s captured on biosensor chemistries, Significantly reduce time to develop antibodies by combining titer and glycan screening • Save FTE costs and perform more projects using the high-throughput Octet® system Cell line development typically includes the screening of thou - FORTEBIO ONLINE STORE Chemical & Consumer Care. Pulp and Paper. Vaccines. SARS-CoV-2 (COVID-19) Vaccine research, testing, and production solutions. From Cell Line Development to Lot Release. Explore our portfolio that supports your drug development process. Products. Protein Analysis. Biosensors, Sensor Chips & Kits. PROTEIN L BIOSENSORS Protein L Biosensors For whole molecule antibody and antibody fragment quantitation Key features • Broader recognition of Ig isotypes and species than Protein A or G • Regenerable and cost-effective format • Binds whole molecule Ab and FAb fragments • Quantitate in crude matrices Overview Dip and Read™ Protein L (ProL) biosensors providea rapid and
CERTIFICATES OF ANALYSIS Certificates of Analysis. Our online CoA search allows easier access to the most up to date certificates for our customers, and makes things more environmentally friendly for all of us. To view your CoA, select the product type, product name and input the lot number listed on your product label. OCTET KINETICS ASSAY: METHOD DEVELOPMENT GUIDELINE 1 1. Purpose 1.1 The purpose of this document is to describe the procedure for developing a ligand binding kinetics assay on Octet® QKe, RED96, RED96e, RED384 and HTX instruments. OCTET SOFTWARE VERSION 10: DATA ACQUISITION AND DATA Octet Data Analysis software version feature comparison Feature V8.x V9.x V10.x Quantitation Mask all proprietary information (sample ID, sensor info, etc.) from data ü ü Editable cells, customize and sort Results table ü ü Import standard curve of experiments run at thesame temperature
OPTIMIZING PROTEIN-PROTEIN AND PROTEIN-SMALL MOLECULE 2 Time (sec) Binding (nm) 0 1.2 2.4 3.6 4.8 0 600 1,200 1,800 2,400 3,000 3,600 4,200 Figure 1: Identifying which of eight solutions is best for regeneration in less than two hours. OCTET POTENCY ASSAY: DEVELOPMENT, QUALIFICATION AND 3 Time (sec) Binding (nm) 0 50 100 150 200 250 300 350 0 0.5 1.0 1.5 2.0 2.5 A1 B1 C1 D1 BIOSENSOR SELECTION Ni-NTA biosensors were selected for this assay for robustness, ANALYSIS OF FC-GAMMA RECEPTOR-IGG INTERACTIONS ON THE Analysis of Fc-gamma Receptor-IgG interactions on the Octet platform Renee Tobias, Danfeng Yao, and Sriram Kumaraswamy, ForteBio Introduction Fc gamma FRAGMENT BASED DRUG DISCOVERY ON PIONEER SYSTEMS USING 1 APPLATO OTE Fragment based drug discovery on Pioneer Systems using Next Generation SPR analysis Eric L. Reese, Ph.D, SensiQ Technologies, Aaron Martin and David O. Apiyo, Ph.D, Marketing ApplicationsManagers, ForteBio
KINETICS OF IRREVERSIBLE INHIBITORS ON THE PIONEER FE 5 Figure 5: SPR data from the Pioneer FE system showing three covalent inhibitors binding target with different C c values. Signal after referencing shows stable baselines, FORTEBIO ONLINE STORE Chemical & Consumer Care. Pulp and Paper. Vaccines. SARS-CoV-2 (COVID-19) Vaccine research, testing, and production solutions. From Cell Line Development to Lot Release. Explore our portfolio that supports your drug development process. Products. Protein Analysis. Biosensors, Sensor Chips & Kits. 8-CHANNEL OCTET SYSTEMS, OCTET RED96E & OCTET QKE The versatile 8-channel Octet systems provide flexibility for rapid assay development. The 8-channel Octet systems perform quantitation of 96 samples in 32 minutes and kinetic screening of 64 samples in 1.5 hours. Analysis can be done using a single channel or up to eight channels, enabling flexibility in sample throughput based on need. ENTRY LEVEL OCTET SYSTEM The Octet® K2 2-channel System brings you unprecedented access to exquisite biomolecular interactions data. Priced for startup academic and biotech labs, low volume users are no longer bound by the trade-off between cost and performance when choosing a label-free assay system. The Octet K2 System characterizes protein-protein andprotein-small
PERSONAL ASSAY SYSTEM The BLItz® system provides a simple, rapid Dip and Read™ approach for protein quantitation in a small, affordably priced personal assay system. With BLItz, proteins and antibodies can be quantitated in a matter of seconds with high specificity and sensitivity, even in crude samples. The system utilizes the same proprietary Bio-LayerSAFETY DATA SHEET
Conforms to Regulation (EC) No. 1907/2006 (REACH), Annex II, as amended by Commission Regulation (EU) 2015/830 - United Kingdom (UK) 1.3 Details of the supplier of the safety data sheetOCTET HTX SYSTEM
Octet HTX System Accelerate your label-free workflow Key features • 96-well plate quantitation in 2 minutes • 32 x 32 epitope binning in less than 8 hours • Full plate off-rate ranking in minutes, not hours • trix that can accommodate a variety of cross-blocking formats Simultaneously reads 8,OCTET QKE SYSTEM
Key features • Increased sensitivity and dynamic range for protein quantitation • Excellent signal resolution for measuring protein and peptide kinetics • Greater operational cost savings with biosensor re-racking and regeneration The Octet® QKe system is the enhanced-performance version of the first-generation Octet QK instrument from the Octet family PROTEIN L BIOSENSORS Protein L Biosensors For whole molecule antibody and antibody fragment quantitation Key features • Broader recognition of Ig isotypes and species than Protein A or G • Regenerable and cost-effective format • Binds whole molecule Ab and FAb fragments • Quantitate in crude matrices Overview Dip and Read™ Protein L (ProL) biosensors providea rapid and
OCTET POTENCY ASSAY: DEVELOPMENT, QUALIFICATION ANDBLI OCTET BINDINGASSAY
3 Time (sec) Binding (nm) 0 50 100 150 200 250 300 350 0 0.5 1.0 1.5 2.0 2.5 A1 B1 C1 D1 BIOSENSOR SELECTION Ni-NTA biosensors were selected for this assay for robustness, CELL LINE DEVELOPMENT: ANTIBODY DISCOVERY BY TITER Key features • BLI measures only what’s captured on biosensor chemistries, Significantly reduce time to develop antibodies by combining titer and glycan screening • Save FTE costs and perform more projects using the high-throughput Octet® system Cell line development typically includes the screening of thou - FORTEBIO ONLINE STORE Chemical & Consumer Care. Pulp and Paper. Vaccines. SARS-CoV-2 (COVID-19) Vaccine research, testing, and production solutions. From Cell Line Development to Lot Release. Explore our portfolio that supports your drug development process. Products. Protein Analysis. Biosensors, Sensor Chips & Kits. 8-CHANNEL OCTET SYSTEMS, OCTET RED96E & OCTET QKE The versatile 8-channel Octet systems provide flexibility for rapid assay development. The 8-channel Octet systems perform quantitation of 96 samples in 32 minutes and kinetic screening of 64 samples in 1.5 hours. Analysis can be done using a single channel or up to eight channels, enabling flexibility in sample throughput based on need. ENTRY LEVEL OCTET SYSTEM The Octet® K2 2-channel System brings you unprecedented access to exquisite biomolecular interactions data. Priced for startup academic and biotech labs, low volume users are no longer bound by the trade-off between cost and performance when choosing a label-free assay system. The Octet K2 System characterizes protein-protein andprotein-small
PERSONAL ASSAY SYSTEM The BLItz® system provides a simple, rapid Dip and Read™ approach for protein quantitation in a small, affordably priced personal assay system. With BLItz, proteins and antibodies can be quantitated in a matter of seconds with high specificity and sensitivity, even in crude samples. The system utilizes the same proprietary Bio-LayerSAFETY DATA SHEET
Conforms to Regulation (EC) No. 1907/2006 (REACH), Annex II, as amended by Commission Regulation (EU) 2015/830 - United Kingdom (UK) 1.3 Details of the supplier of the safety data sheetOCTET HTX SYSTEM
Octet HTX System Accelerate your label-free workflow Key features • 96-well plate quantitation in 2 minutes • 32 x 32 epitope binning in less than 8 hours • Full plate off-rate ranking in minutes, not hours • trix that can accommodate a variety of cross-blocking formats Simultaneously reads 8,OCTET QKE SYSTEM
Key features • Increased sensitivity and dynamic range for protein quantitation • Excellent signal resolution for measuring protein and peptide kinetics • Greater operational cost savings with biosensor re-racking and regeneration The Octet® QKe system is the enhanced-performance version of the first-generation Octet QK instrument from the Octet family PROTEIN L BIOSENSORS Protein L Biosensors For whole molecule antibody and antibody fragment quantitation Key features • Broader recognition of Ig isotypes and species than Protein A or G • Regenerable and cost-effective format • Binds whole molecule Ab and FAb fragments • Quantitate in crude matrices Overview Dip and Read™ Protein L (ProL) biosensors providea rapid and
OCTET POTENCY ASSAY: DEVELOPMENT, QUALIFICATION ANDBLI OCTET BINDINGASSAY
3 Time (sec) Binding (nm) 0 50 100 150 200 250 300 350 0 0.5 1.0 1.5 2.0 2.5 A1 B1 C1 D1 BIOSENSOR SELECTION Ni-NTA biosensors were selected for this assay for robustness, CELL LINE DEVELOPMENT: ANTIBODY DISCOVERY BY TITER Key features • BLI measures only what’s captured on biosensor chemistries, Significantly reduce time to develop antibodies by combining titer and glycan screening • Save FTE costs and perform more projects using the high-throughput Octet® system Cell line development typically includes the screening of thou - FORTEBIO ONLINE STORE Chemical & Consumer Care. Pulp and Paper. Vaccines. SARS-CoV-2 (COVID-19) Vaccine research, testing, and production solutions. From Cell Line Development to Lot Release. Explore our portfolio that supports your drug development process. Products. Protein Analysis. Biosensors, Sensor Chips & Kits. PROTEIN L BIOSENSORS Protein L Biosensors For whole molecule antibody and antibody fragment quantitation Key features • Broader recognition of Ig isotypes and species than Protein A or G • Regenerable and cost-effective format • Binds whole molecule Ab and FAb fragments • Quantitate in crude matrices Overview Dip and Read™ Protein L (ProL) biosensors providea rapid and
CERTIFICATES OF ANALYSIS Certificates of Analysis. Our online CoA search allows easier access to the most up to date certificates for our customers, and makes things more environmentally friendly for all of us. To view your CoA, select the product type, product name and input the lot number listed on your product label. OCTET KINETICS ASSAY: METHOD DEVELOPMENT GUIDELINE 1 1. Purpose 1.1 The purpose of this document is to describe the procedure for developing a ligand binding kinetics assay on Octet® QKe, RED96, RED96e, RED384 and HTX instruments. OCTET SOFTWARE VERSION 10: DATA ACQUISITION AND DATA Octet Data Analysis software version feature comparison Feature V8.x V9.x V10.x Quantitation Mask all proprietary information (sample ID, sensor info, etc.) from data ü ü Editable cells, customize and sort Results table ü ü Import standard curve of experiments run at thesame temperature
OPTIMIZING PROTEIN-PROTEIN AND PROTEIN-SMALL MOLECULE 2 Time (sec) Binding (nm) 0 1.2 2.4 3.6 4.8 0 600 1,200 1,800 2,400 3,000 3,600 4,200 Figure 1: Identifying which of eight solutions is best for regeneration in less than two hours. OCTET POTENCY ASSAY: DEVELOPMENT, QUALIFICATION AND 3 Time (sec) Binding (nm) 0 50 100 150 200 250 300 350 0 0.5 1.0 1.5 2.0 2.5 A1 B1 C1 D1 BIOSENSOR SELECTION Ni-NTA biosensors were selected for this assay for robustness, ANALYSIS OF FC-GAMMA RECEPTOR-IGG INTERACTIONS ON THE Analysis of Fc-gamma Receptor-IgG interactions on the Octet platform Renee Tobias, Danfeng Yao, and Sriram Kumaraswamy, ForteBio Introduction Fc gamma FRAGMENT BASED DRUG DISCOVERY ON PIONEER SYSTEMS USING 1 APPLATO OTE Fragment based drug discovery on Pioneer Systems using Next Generation SPR analysis Eric L. Reese, Ph.D, SensiQ Technologies, Aaron Martin and David O. Apiyo, Ph.D, Marketing ApplicationsManagers, ForteBio
KINETICS OF IRREVERSIBLE INHIBITORS ON THE PIONEER FE 5 Figure 5: SPR data from the Pioneer FE system showing three covalent inhibitors binding target with different C c values. Signal after referencing shows stable baselines, FORTEBIO ONLINE STORE Choosing a selection results in a full page refresh. Press the space key then arrow keys to make a selection. 8-CHANNEL OCTET SYSTEMS, OCTET RED96E & OCTET QKE The versatile 8-channel Octet systems provide flexibility for rapid assay development. The 8-channel Octet systems perform quantitation of96 samples in 32
ENTRY LEVEL OCTET SYSTEM Octet K2 System. The Octet® K2 2-channel System brings you unprecedented access to exquisite biomolecular interactions data. Priced for startup academic and biotech labs, low volume users are no longer bound by the trade-off between cost and performance when choosing a label-free assay system.SAFETY DATA SHEET
Conforms to Regulation (EC) No. 1907/2006 (REACH), Annex II, as amended by Commission Regulation (EU) 2015/830 - United Kingdom (UK) 1.3 Details of the supplier of the safety data sheet PERSONAL ASSAY SYSTEM Drop. Read. Done. The BLItz® System brings label-free analysis to individual researchers, and is affordably priced so everyone can have one. With this personal assay system, you can run protein quantitation and kinetics experiments in only 4 µL of sample—right at your ownlab bench.
OCTET HTX SYSTEM
Octet HTX System Accelerate your label-free workflow Key features • 96-well plate quantitation in 2 minutes • 32 x 32 epitope binning in less than 8 hours • Full plate off-rate ranking in minutes, not hours • trix that can accommodate a variety of cross-blocking formats Simultaneously reads 8,OCTET QKE SYSTEM
Key features • Increased sensitivity and dynamic range for protein quantitation • Excellent signal resolution for measuring protein and peptide kinetics • Greater operational cost savings with biosensor re-racking and regeneration The Octet® QKe system is the enhanced-performance version of the first-generation Octet QK instrument from the Octet family PROTEIN L BIOSENSORS Protein L Biosensors For whole molecule antibody and antibody fragment quantitation Key features • Broader recognition of Ig isotypes and species than Protein A or G • Regenerable and cost-effective format • Binds whole molecule Ab and FAb fragments • Quantitate in crude matrices Overview Dip and Read™ Protein L (ProL) biosensors providea rapid and
OCTET POTENCY ASSAY: DEVELOPMENT, QUALIFICATION ANDBLI OCTET BINDINGASSAY
3 Time (sec) Binding (nm) 0 50 100 150 200 250 300 350 0 0.5 1.0 1.5 2.0 2.5 A1 B1 C1 D1 BIOSENSOR SELECTION Ni-NTA biosensors were selected for this assay for robustness, CELL LINE DEVELOPMENT: ANTIBODY DISCOVERY BY TITER Key features • BLI measures only what’s captured on biosensor chemistries, Significantly reduce time to develop antibodies by combining titer and glycan screening • Save FTE costs and perform more projects using the high-throughput Octet® system Cell line development typically includes the screening of thou - FORTEBIO ONLINE STORE Choosing a selection results in a full page refresh. Press the space key then arrow keys to make a selection. 8-CHANNEL OCTET SYSTEMS, OCTET RED96E & OCTET QKE The versatile 8-channel Octet systems provide flexibility for rapid assay development. The 8-channel Octet systems perform quantitation of96 samples in 32
ENTRY LEVEL OCTET SYSTEM Octet K2 System. The Octet® K2 2-channel System brings you unprecedented access to exquisite biomolecular interactions data. Priced for startup academic and biotech labs, low volume users are no longer bound by the trade-off between cost and performance when choosing a label-free assay system.SAFETY DATA SHEET
Conforms to Regulation (EC) No. 1907/2006 (REACH), Annex II, as amended by Commission Regulation (EU) 2015/830 - United Kingdom (UK) 1.3 Details of the supplier of the safety data sheet PERSONAL ASSAY SYSTEM Drop. Read. Done. The BLItz® System brings label-free analysis to individual researchers, and is affordably priced so everyone can have one. With this personal assay system, you can run protein quantitation and kinetics experiments in only 4 µL of sample—right at your ownlab bench.
OCTET HTX SYSTEM
Octet HTX System Accelerate your label-free workflow Key features • 96-well plate quantitation in 2 minutes • 32 x 32 epitope binning in less than 8 hours • Full plate off-rate ranking in minutes, not hours • trix that can accommodate a variety of cross-blocking formats Simultaneously reads 8,OCTET QKE SYSTEM
Key features • Increased sensitivity and dynamic range for protein quantitation • Excellent signal resolution for measuring protein and peptide kinetics • Greater operational cost savings with biosensor re-racking and regeneration The Octet® QKe system is the enhanced-performance version of the first-generation Octet QK instrument from the Octet family PROTEIN L BIOSENSORS Protein L Biosensors For whole molecule antibody and antibody fragment quantitation Key features • Broader recognition of Ig isotypes and species than Protein A or G • Regenerable and cost-effective format • Binds whole molecule Ab and FAb fragments • Quantitate in crude matrices Overview Dip and Read™ Protein L (ProL) biosensors providea rapid and
OCTET POTENCY ASSAY: DEVELOPMENT, QUALIFICATION ANDBLI OCTET BINDINGASSAY
3 Time (sec) Binding (nm) 0 50 100 150 200 250 300 350 0 0.5 1.0 1.5 2.0 2.5 A1 B1 C1 D1 BIOSENSOR SELECTION Ni-NTA biosensors were selected for this assay for robustness, CELL LINE DEVELOPMENT: ANTIBODY DISCOVERY BY TITER Key features • BLI measures only what’s captured on biosensor chemistries, Significantly reduce time to develop antibodies by combining titer and glycan screening • Save FTE costs and perform more projects using the high-throughput Octet® system Cell line development typically includes the screening of thou - FORTEBIO ONLINE STORE Choosing a selection results in a full page refresh. Press the space key then arrow keys to make a selection. PROTEIN L BIOSENSORS Protein L Biosensors For whole molecule antibody and antibody fragment quantitation Key features • Broader recognition of Ig isotypes and species than Protein A or G • Regenerable and cost-effective format • Binds whole molecule Ab and FAb fragments • Quantitate in crude matrices Overview Dip and Read™ Protein L (ProL) biosensors providea rapid and
CERTIFICATES OF ANALYSIS Our online CoA search allows easier access to the most up to date certificates for our customers, and makes things more environmentally friendly for all of us. To view your CoA, select the product type, product name and input the lot number listed on your product label. How can I find my lot number? OCTET SOFTWARE VERSION 10: DATA ACQUISITION AND DATA Octet Data Analysis software version feature comparison Feature V8.x V9.x V10.x Quantitation Mask all proprietary information (sample ID, sensor info, etc.) from data ü ü Editable cells, customize and sort Results table ü ü Import standard curve of experiments run at thesame temperature
OCTET KINETICS ASSAY: METHOD DEVELOPMENT GUIDELINE 1 1. Purpose 1.1 The purpose of this document is to describe the procedure for developing a ligand binding kinetics assay on Octet® QKe, RED96, RED96e, RED384 and HTX instruments. OPTIMIZING PROTEIN-PROTEIN AND PROTEIN-SMALL MOLECULE 2 Time (sec) Binding (nm) 0 1.2 2.4 3.6 4.8 0 600 1,200 1,800 2,400 3,000 3,600 4,200 Figure 1: Identifying which of eight solutions is best for regeneration in less than two hours. OCTET POTENCY ASSAY: DEVELOPMENT, QUALIFICATION AND 3 Time (sec) Binding (nm) 0 50 100 150 200 250 300 350 0 0.5 1.0 1.5 2.0 2.5 A1 B1 C1 D1 BIOSENSOR SELECTION Ni-NTA biosensors were selected for this assay for robustness, ANALYSIS OF FC-GAMMA RECEPTOR-IGG INTERACTIONS ON THE Analysis of Fc-gamma Receptor-IgG interactions on the Octet platform Renee Tobias, Danfeng Yao, and Sriram Kumaraswamy, ForteBio Introduction Fc gamma FRAGMENT BASED DRUG DISCOVERY ON PIONEER SYSTEMS USING 1 APPLATO OTE Fragment based drug discovery on Pioneer Systems using Next Generation SPR analysis Eric L. Reese, Ph.D, SensiQ Technologies, Aaron Martin and David O. Apiyo, Ph.D, Marketing ApplicationsManagers, ForteBio
KINETICS OF IRREVERSIBLE INHIBITORS ON THE PIONEER FE 5 Figure 5: SPR data from the Pioneer FE system showing three covalent inhibitors binding target with different C c values. Signal after referencing shows stable baselines, Skip to main contentToggle navigation
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BIOLOGICS BY FORTEBIO Real-time analysis of molecular interactions with fluidics-free BLI and next generation SPR label-free detection systems BLI Systems SPR Systems*
BIOLOGICS BY FORTEBIO Real-time analysis of molecular interactions with fluidics-free BLI and next generation SPR label-free detection systems BLI Systems SPR Systems*
BIOLOGICS BY FORTEBIO Real-time analysis of molecular interactions with fluidics-free BLI and next generation SPR label-free detection systems BLI Systems SPR Systems* Previous
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RELY ON THE INDUSTRY STANDARD FOR LABEL-FREE ANALYSIS ACROSS BIOLOGICS DEVELOPMENT AND MANUFACTURING * CONTINUOUS INNOVATION As a trusted scientific partner, we find solutions to research challenges by providing best-in-class products for real-time analysis of biomolecular interactions in micro-volume sample sizes, providing information on affinity, kinetics, and concentration. * EXTENSIVE PUBLICATION SUCCESS ForteBio systems have become indispensable tools in academic and biopharmaceutical research, from characterizing binding interactions and specificity to engineering proteins and antibodies. * WORLD CLASS SERVICE PLANS Comprehensive service plans include validation and preventative maintenance that reduce operating costs and maximize lab productivity.Read more
OUR FEATURED PRODUCTS HIGH PRECISION STREPTAVIDIN 2.0 (SAX2) BIOSENSORS For high precision and reproducible kinetic characterization andcustom quantitation
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OCTET HTX SYSTEM
High throughput biologics characterization – affinity, quantitation,and more
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PIONEER FE SPR SYSTEM Make Decisions Earlier in Fragment Screens with the Pioneer FEView Product _ _
OUR CUSTOMER STORIES UNIVERSITY OF BRITISH COLUMBIA USES THE OCTET RED96E SYSTEM TO QUICKLY DETERMINE INTERACTION KINETICSDetails
10,000 BINDING EVENTS PER DAY AT AVITIDEDetails
LIPOSOME-PROTEIN INTERACTIONS ON THE OCTET RED96E PLATFORMDetails
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