Are you over 18 and want to see adult content?
More Annotations
A complete backup of dent-rep-servisteam.cz
Are you over 18 and want to see adult content?
A complete backup of grand-casino.com
Are you over 18 and want to see adult content?
A complete backup of songmeanings.com
Are you over 18 and want to see adult content?
A complete backup of tonyschocolonely.com
Are you over 18 and want to see adult content?
A complete backup of patriciapolacco.com
Are you over 18 and want to see adult content?
A complete backup of kreissl-morbach.de
Are you over 18 and want to see adult content?
A complete backup of alkamotorkia.com.co
Are you over 18 and want to see adult content?
Favourite Annotations
INFORMACION.ES: Noticias de Alicante, Comunidad Valenciana, Deportes, España
Are you over 18 and want to see adult content?
Gary Sieling – Software Architect – Ambler, PA
Are you over 18 and want to see adult content?
Van Santvoort Landelijk Wonen
Are you over 18 and want to see adult content?
Easy & Free Website Maker | Create a Free Website - Webnode
Are you over 18 and want to see adult content?
Hotel-Gasthof Huettensteinach
Are you over 18 and want to see adult content?
Text
domain.
PLANT ELECTROPHYSIOLOGY Preface Plant electrophysiology is the study of the electrochemical phenomena associated with biological cells and tissues in plants. It involves measurements of electrical PHOTOINITIATORS FOR UVCURING 200 220 240 260 280 300 320 340 1.2 1.0 0.8 0.6 0.4 0.2 0.0 Key products, surface cure Absorption Spectra 0.001% (Acetonitrile) Wavelength (nm) Absorbance IRGACURE 184 DAROCUR 1173 IRGACURE 754 IRGACURE 2959 200 220 240 260 280 300 320 340 360 380 POLYETHYLENE GLYCOL-COATED BIOCOMPATIBLE SURFACES Polyethylene glycol–coated biocompatible surfaces Norma A. Alcantar, Eray S. Aydil, Jacob N. Israelachvili Chemical Engineering Department and Materials Department, University of California, Santa Barbara,California 93106
YEAST CELL CYCLE FLOW CYTOMETRY PROTOCOL Protocol. Spin down 10 7 cells from an exponentially growing culture - 2000 rpm for 5 mins. Pour off supernatant. Vortex tube while adding 1.0 ml cold 70% EtOH. Store at 4 °C (cells keep ~indefinitely). When you want to process the cells, take 0.3 ml (this will be 2-3 x 10 6 cells, assuming a little loss in the washing) and add to 3 ml 50 mM ADHESION ASSAY PROTOCOL Adhesion Assay Protocol: Materials to be prepared beforehand: 1) Washing Buffer--0.1% BSA in medium (DMEM or RPMI) 2) Blocking Buffer--0.5% BSA in medium (DMEM or RPMI) EDUCATION GUIDE SPECIAL STAINS 1 Introduction to Special Stains Sonja Wulff Thousands of years ago, diagnosing and treating a patient’s condition was much simpler. Ancient Egyptians blamed disease on demons, Greeks and Romans pointed THMR-IP5680 HP I-LINE PHOTORESIST 4 26-WE4C03-00 OHKA AMERICA, INC. 300 400 500 600 700 800 900 300 400 500 600 700 800 900 Mask CD (nm) Print CD (nm) THMR-iP5680 HP Target CD = 0.6 µm, exposure dose = 160 ms EFFECT OF TEMPERATURE VARIATION ON THE VISIBLE AND NEAR 226 D. Cozzolino et al. / Analytica Chimica Acta 588 (2007) 224–230 Four wavelength regions were analysed in order to spectrally evaluate the effect of the temperature on the NIR wine spec-trum. These regions were, 950–1000nm, 1410–1470nm related 2D PROCESS MODELING WITH SILVACO ATHENA DR. LYNN FULLER Page 6 © April 30, 2014 Dr. Lynn Fuller, Professor Process Modeling - TCAD Page 11 SILVACO ATHENA SIMULATIONS OF D/S IMPLANT © April 30, 2014 Dr. Lynn Fuller, Professor BASIC LABORATORY PRACTICES Basic Laboratory Practices. General Method for Cleavage of His-Tagged Proteins with Thrombin cleavage sites . Thrombin recognises the consensus sequence Leu-Val-Pro-Arg-Gly-Ser, cleaving the peptide bond between Arg and Gly. This is utilised in many vector systems which encode such a protease cleavage site allowing removal of an upstreamdomain.
PLANT ELECTROPHYSIOLOGY Preface Plant electrophysiology is the study of the electrochemical phenomena associated with biological cells and tissues in plants. It involves measurements of electrical PHOTOINITIATORS FOR UVCURING 200 220 240 260 280 300 320 340 1.2 1.0 0.8 0.6 0.4 0.2 0.0 Key products, surface cure Absorption Spectra 0.001% (Acetonitrile) Wavelength (nm) Absorbance IRGACURE 184 DAROCUR 1173 IRGACURE 754 IRGACURE 2959 200 220 240 260 280 300 320 340 360 380 POLYETHYLENE GLYCOL-COATED BIOCOMPATIBLE SURFACES Polyethylene glycol–coated biocompatible surfaces Norma A. Alcantar, Eray S. Aydil, Jacob N. Israelachvili Chemical Engineering Department and Materials Department, University of California, Santa Barbara,California 93106
YEAST CELL CYCLE FLOW CYTOMETRY PROTOCOL Protocol. Spin down 10 7 cells from an exponentially growing culture - 2000 rpm for 5 mins. Pour off supernatant. Vortex tube while adding 1.0 ml cold 70% EtOH. Store at 4 °C (cells keep ~indefinitely). When you want to process the cells, take 0.3 ml (this will be 2-3 x 10 6 cells, assuming a little loss in the washing) and add to 3 ml 50 mM ADHESION ASSAY PROTOCOL Adhesion Assay Protocol: Materials to be prepared beforehand: 1) Washing Buffer--0.1% BSA in medium (DMEM or RPMI) 2) Blocking Buffer--0.5% BSA in medium (DMEM or RPMI) EDUCATION GUIDE SPECIAL STAINS 1 Introduction to Special Stains Sonja Wulff Thousands of years ago, diagnosing and treating a patient’s condition was much simpler. Ancient Egyptians blamed disease on demons, Greeks and Romans pointed THMR-IP5680 HP I-LINE PHOTORESIST 4 26-WE4C03-00 OHKA AMERICA, INC. 300 400 500 600 700 800 900 300 400 500 600 700 800 900 Mask CD (nm) Print CD (nm) THMR-iP5680 HP Target CD = 0.6 µm, exposure dose = 160 ms EFFECT OF TEMPERATURE VARIATION ON THE VISIBLE AND NEAR 226 D. Cozzolino et al. / Analytica Chimica Acta 588 (2007) 224–230 Four wavelength regions were analysed in order to spectrally evaluate the effect of the temperature on the NIR wine spec-trum. These regions were, 950–1000nm, 1410–1470nm related 2D PROCESS MODELING WITH SILVACO ATHENA DR. LYNN FULLER Page 6 © April 30, 2014 Dr. Lynn Fuller, Professor Process Modeling - TCAD Page 11 SILVACO ATHENA SIMULATIONS OF D/S IMPLANT © April 30, 2014 Dr. Lynn Fuller, ProfessorPCR TROUBLESHOOTING
Increase primer amount. Increase template amount. Decrease annealing temperature by 6-10º C and check if you get any product. If you don't, check all your PCR ingredients. If you do get products (including unspecific ones) reaction conditions as described above. Combine some/all of the above. 4. YEAST CELL CYCLE FLOW CYTOMETRY PROTOCOL Protocol. Spin down 10 7 cells from an exponentially growing culture - 2000 rpm for 5 mins. Pour off supernatant. Vortex tube while adding 1.0 ml cold 70% EtOH. Store at 4 °C (cells keep ~indefinitely). When you want to process the cells, take 0.3 ml (this will be 2-3 x 10 6 cells, assuming a little loss in the washing) and add to 3 ml 50 mM PROTEIN DETERMINATION BY THE BIURET METHOD Prepare a reference tube with 1 ml buffer. If possible, dilute unknowns to an estimated 1 to 10 mg/ml with buffer; a range of dilutions should be used if the actual concentration cannot be estimated. Use 1 ml sample per assay tube. Add 9 ml Biuret reagent to each tube, vortex immediately, and EDUCATION GUIDE SPECIAL STAINS 1 Introduction to Special Stains Sonja Wulff Thousands of years ago, diagnosing and treating a patient’s condition was much simpler. Ancient Egyptians blamed disease on demons, Greeks and Romans pointed THMR-IP5680 HP I-LINE PHOTORESIST 4 26-WE4C03-00 OHKA AMERICA, INC. 300 400 500 600 700 800 900 300 400 500 600 700 800 900 Mask CD (nm) Print CD (nm) THMR-iP5680 HP Target CD = 0.6 µm, exposure dose = 160 msOPEN-SPECTROMETER
design files for a low-cost open source photospectrometer: root: summary refs log tree commit diff SPIN COATING: ONEDIMENSIONAL MODEL Spin coating: Onedimensional model D. E. Bornside, C. W. Macosko, and L. E. Scriven Citation: Journal of Applied Physics 66, 5185 (1989); doi: 10.1063/1.343754PINY-COMMANDS
CGI forms for Piny: root: summary refs log tree commit diff INTRODUCTION TO ION IMPLANTATION DR. LYNN FULLER, DR January 20, 2012 Dr. Lynn Fuller Rochester Institute of Technology Microelectronic Engineering Ion Implantation Page 2 VARIAN 400 & 120-10 ION IMPLANTERS FREQUENCY RESPONSE OF THE CE AMPLIFIER September 25, 2014 Dr. Lynn Fuller, Professor Rochester Institute of Technology Microelectronic Engineering Frequency Response Page 3INTRODUCTION
BASIC LABORATORY PRACTICES Basic Laboratory Practices. General Method for Cleavage of His-Tagged Proteins with Thrombin cleavage sites . Thrombin recognises the consensus sequence Leu-Val-Pro-Arg-Gly-Ser, cleaving the peptide bond between Arg and Gly. This is utilised in many vector systems which encode such a protease cleavage site allowing removal of an upstreamdomain.
EISENLAB - DIYHPL.US Maple Tree is a java-based, open source, cross-platform visualization tool to graphically browse the results of clustering analyses from our Cluster and Fuzzy K clustering software, and many other clustering and analysis programs. Maple Tree was developed by Lisa Simirenko in our lab. Combined Expression Data and Sequence Analysis. EDUCATION GUIDE SPECIAL STAINS 1 Introduction to Special Stains Sonja Wulff Thousands of years ago, diagnosing and treating a patient’s condition was much simpler. Ancient Egyptians blamed disease on demons, Greeks and Romans pointed YEAST CELL CYCLE FLOW CYTOMETRY PROTOCOL Protocol. Spin down 10 7 cells from an exponentially growing culture - 2000 rpm for 5 mins. Pour off supernatant. Vortex tube while adding 1.0 ml cold 70% EtOH. Store at 4 °C (cells keep ~indefinitely). When you want to process the cells, take 0.3 ml (this will be 2-3 x 10 6 cells, assuming a little loss in the washing) and add to 3 ml 50 mM A LOW-COST, OPEN-SOURCE DIY DNA SYNTHESIZER PROJECT Building low-cost DNA synthesizers Our project has multiple phases, but the most interesting phase is the microfluidic chip. Microscopic channels guide the direction of fluids and reagents. Version 1: phosphoramidite chemistry. Version “the next one”: ligation chemistry from on- chip 4,096 droplet library (synthesis by shortoligo
GROWTH AND CHARACTERIZATION OF (110) INAS QUANTUM WELL Growth and Characterization of (110) InAs Quantum Well Heterostructures by Transmission Electron Microscopy and Electron Channeling Contrast Imaging. CLINICAL DEVELOPMENT SUCCESS RATES FOR INVESTIGATIONAL DRUGS 42 volume 32 NumBeR 1 JANuARY 2014 nature biotechnology data to annotate drugs by their biochemi-cal composition (e.g., peptide, nucleic acid, monoclonal antibody (mAb)) and molecu-lar size (i.e., large and small molecules). ADVANTAGES OF BEAM DECELERATION FOR LOW KV EDS ANALYSIS. Figure 1. EDS map of Slag at 3kV and 90nA: (a,c,e) No Gentle Beam; (b,d,f) -5kV Gentle Beam. a b e f c d a Microsc. Microanal. 21 (Suppl3), 2015 682
A PATHETICALLY SIMPLE CHARGE SENSITIVE AMPLIFIER A Pathetically Simple Charge Sensitive Amplifier The schematic above shows an extremely simple charge sensitive amplifier with only twoactive components.
HIGH SPEED LOGIC CIRCUITS DR. LYNN FULLER November 5, 2014 Dr. Lynn Fuller High Speed Logic Page 3 Rochester Institute of Technology Microelectronic Engineering OUTLINEIntroduction
BASIC LABORATORY PRACTICES Basic Laboratory Practices. General Method for Cleavage of His-Tagged Proteins with Thrombin cleavage sites . Thrombin recognises the consensus sequence Leu-Val-Pro-Arg-Gly-Ser, cleaving the peptide bond between Arg and Gly. This is utilised in many vector systems which encode such a protease cleavage site allowing removal of an upstreamdomain.
PCR TROUBLESHOOTING
Increase primer amount. Increase template amount. Decrease annealing temperature by 6-10º C and check if you get any product. If you don't, check all your PCR ingredients. If you do get products (including unspecific ones) reaction conditions as described above. Combine some/all of the above. 4. YEAST CELL CYCLE FLOW CYTOMETRY PROTOCOL Protocol. Spin down 10 7 cells from an exponentially growing culture - 2000 rpm for 5 mins. Pour off supernatant. Vortex tube while adding 1.0 ml cold 70% EtOH. Store at 4 °C (cells keep ~indefinitely). When you want to process the cells, take 0.3 ml (this will be 2-3 x 10 6 cells, assuming a little loss in the washing) and add to 3 ml 50 mM THMR-IP5680 HP I-LINE PHOTORESIST 4 26-WE4C03-00 OHKA AMERICA, INC. 300 400 500 600 700 800 900 300 400 500 600 700 800 900 Mask CD (nm) Print CD (nm) THMR-iP5680 HP Target CD = 0.6 µm, exposure dose = 160 ms EDUCATION GUIDE SPECIAL STAINS 1 Introduction to Special Stains Sonja Wulff Thousands of years ago, diagnosing and treating a patient’s condition was much simpler. Ancient Egyptians blamed disease on demons, Greeks and Romans pointed A LOW-COST, OPEN-SOURCE DIY DNA SYNTHESIZER PROJECT Building low-cost DNA synthesizers Our project has multiple phases, but the most interesting phase is the microfluidic chip. Microscopic channels guide the direction of fluids and reagents. Version 1: phosphoramidite chemistry. Version “the next one”: ligation chemistry from on- chip 4,096 droplet library (synthesis by shortoligo
GROWTH AND CHARACTERIZATION OF (110) INAS QUANTUM WELL Growth and Characterization of (110) InAs Quantum Well Heterostructures by Transmission Electron Microscopy and Electron Channeling Contrast Imaging. CLINICAL DEVELOPMENT SUCCESS RATES FOR INVESTIGATIONAL DRUGS 42 volume 32 NumBeR 1 JANuARY 2014 nature biotechnology data to annotate drugs by their biochemi-cal composition (e.g., peptide, nucleic acid, monoclonal antibody (mAb)) and molecu-lar size (i.e., large and small molecules). ADVANTAGES OF BEAM DECELERATION FOR LOW KV EDS ANALYSIS. Figure 1. EDS map of Slag at 3kV and 90nA: (a,c,e) No Gentle Beam; (b,d,f) -5kV Gentle Beam. a b e f c d a Microsc. Microanal. 21 (Suppl3), 2015 682
HIGH SPEED LOGIC CIRCUITS DR. LYNN FULLER November 5, 2014 Dr. Lynn Fuller High Speed Logic Page 3 Rochester Institute of Technology Microelectronic Engineering OUTLINEIntroduction
EISENLAB - DIYHPL.US Maple Tree is a java-based, open source, cross-platform visualization tool to graphically browse the results of clustering analyses from our Cluster and Fuzzy K clustering software, and many other clustering and analysis programs. Maple Tree was developed by Lisa Simirenko in our lab. Combined Expression Data and Sequence Analysis.PCR TROUBLESHOOTING
Increase primer amount. Increase template amount. Decrease annealing temperature by 6-10º C and check if you get any product. If you don't, check all your PCR ingredients. If you do get products (including unspecific ones) reaction conditions as described above. Combine some/all of the above. 4. YEAST CELL CYCLE FLOW CYTOMETRY PROTOCOL Protocol. Spin down 10 7 cells from an exponentially growing culture - 2000 rpm for 5 mins. Pour off supernatant. Vortex tube while adding 1.0 ml cold 70% EtOH. Store at 4 °C (cells keep ~indefinitely). When you want to process the cells, take 0.3 ml (this will be 2-3 x 10 6 cells, assuming a little loss in the washing) and add to 3 ml 50 mM PROTEIN DETERMINATION BY THE BIURET METHOD Prepare a reference tube with 1 ml buffer. If possible, dilute unknowns to an estimated 1 to 10 mg/ml with buffer; a range of dilutions should be used if the actual concentration cannot be estimated. Use 1 ml sample per assay tube. Add 9 ml Biuret reagent to each tube, vortex immediately, and PHOTOINITIATORS FOR UVCURING 200 220 240 260 280 300 320 340 1.2 1.0 0.8 0.6 0.4 0.2 0.0 Key products, surface cure Absorption Spectra 0.001% (Acetonitrile) Wavelength (nm) Absorbance IRGACURE 184 DAROCUR 1173 IRGACURE 754 IRGACURE 2959 200 220 240 260 280 300 320 340 360 380 POLYETHYLENE GLYCOL-COATED BIOCOMPATIBLE SURFACES Polyethylene glycol–coated biocompatible surfaces Norma A. Alcantar, Eray S. Aydil, Jacob N. Israelachvili Chemical Engineering Department and Materials Department, University of California, Santa Barbara,California 93106
EDUCATION GUIDE SPECIAL STAINS 1 Introduction to Special Stains Sonja Wulff Thousands of years ago, diagnosing and treating a patient’s condition was much simpler. Ancient Egyptians blamed disease on demons, Greeks and Romans pointedOPEN-SPECTROMETER
design files for a low-cost open source photospectrometer: root: summary refs log tree commit diff PORTLAB - BIOHACKING WIKI FOR PORTLAND, OREGON. Biohacking wiki for Portland, Oregon. root: summary refs log treecommit diff
PINY-COMMANDS
CGI forms for Piny: root: summary refs log tree commit diff BASIC LABORATORY PRACTICES Basic Laboratory Practices. General Method for Cleavage of His-Tagged Proteins with Thrombin cleavage sites . Thrombin recognises the consensus sequence Leu-Val-Pro-Arg-Gly-Ser, cleaving the peptide bond between Arg and Gly. This is utilised in many vector systems which encode such a protease cleavage site allowing removal of an upstreamdomain.
EISENLAB - DIYHPL.US Maple Tree is a java-based, open source, cross-platform visualization tool to graphically browse the results of clustering analyses from our Cluster and Fuzzy K clustering software, and many other clustering and analysis programs. Maple Tree was developed by Lisa Simirenko in our lab. Combined Expression Data and Sequence Analysis. EDUCATION GUIDE SPECIAL STAINS 1 Introduction to Special Stains Sonja Wulff Thousands of years ago, diagnosing and treating a patient’s condition was much simpler. Ancient Egyptians blamed disease on demons, Greeks and Romans pointed YEAST CELL CYCLE FLOW CYTOMETRY PROTOCOL Protocol. Spin down 10 7 cells from an exponentially growing culture - 2000 rpm for 5 mins. Pour off supernatant. Vortex tube while adding 1.0 ml cold 70% EtOH. Store at 4 °C (cells keep ~indefinitely). When you want to process the cells, take 0.3 ml (this will be 2-3 x 10 6 cells, assuming a little loss in the washing) and add to 3 ml 50 mM A LOW-COST, OPEN-SOURCE DIY DNA SYNTHESIZER PROJECT Building low-cost DNA synthesizers Our project has multiple phases, but the most interesting phase is the microfluidic chip. Microscopic channels guide the direction of fluids and reagents. Version 1: phosphoramidite chemistry. Version “the next one”: ligation chemistry from on- chip 4,096 droplet library (synthesis by shortoligo
GROWTH AND CHARACTERIZATION OF (110) INAS QUANTUM WELL Growth and Characterization of (110) InAs Quantum Well Heterostructures by Transmission Electron Microscopy and Electron Channeling Contrast Imaging. CLINICAL DEVELOPMENT SUCCESS RATES FOR INVESTIGATIONAL DRUGS 42 volume 32 NumBeR 1 JANuARY 2014 nature biotechnology data to annotate drugs by their biochemi-cal composition (e.g., peptide, nucleic acid, monoclonal antibody (mAb)) and molecu-lar size (i.e., large and small molecules). ADVANTAGES OF BEAM DECELERATION FOR LOW KV EDS ANALYSIS. Figure 1. EDS map of Slag at 3kV and 90nA: (a,c,e) No Gentle Beam; (b,d,f) -5kV Gentle Beam. a b e f c d a Microsc. Microanal. 21 (Suppl3), 2015 682
A PATHETICALLY SIMPLE CHARGE SENSITIVE AMPLIFIER A Pathetically Simple Charge Sensitive Amplifier The schematic above shows an extremely simple charge sensitive amplifier with only twoactive components.
HIGH SPEED LOGIC CIRCUITS DR. LYNN FULLER November 5, 2014 Dr. Lynn Fuller High Speed Logic Page 3 Rochester Institute of Technology Microelectronic Engineering OUTLINEIntroduction
BASIC LABORATORY PRACTICES Basic Laboratory Practices. General Method for Cleavage of His-Tagged Proteins with Thrombin cleavage sites . Thrombin recognises the consensus sequence Leu-Val-Pro-Arg-Gly-Ser, cleaving the peptide bond between Arg and Gly. This is utilised in many vector systems which encode such a protease cleavage site allowing removal of an upstreamdomain.
EISENLAB - DIYHPL.US Maple Tree is a java-based, open source, cross-platform visualization tool to graphically browse the results of clustering analyses from our Cluster and Fuzzy K clustering software, and many other clustering and analysis programs. Maple Tree was developed by Lisa Simirenko in our lab. Combined Expression Data and Sequence Analysis. EDUCATION GUIDE SPECIAL STAINS 1 Introduction to Special Stains Sonja Wulff Thousands of years ago, diagnosing and treating a patient’s condition was much simpler. Ancient Egyptians blamed disease on demons, Greeks and Romans pointed YEAST CELL CYCLE FLOW CYTOMETRY PROTOCOL Protocol. Spin down 10 7 cells from an exponentially growing culture - 2000 rpm for 5 mins. Pour off supernatant. Vortex tube while adding 1.0 ml cold 70% EtOH. Store at 4 °C (cells keep ~indefinitely). When you want to process the cells, take 0.3 ml (this will be 2-3 x 10 6 cells, assuming a little loss in the washing) and add to 3 ml 50 mM A LOW-COST, OPEN-SOURCE DIY DNA SYNTHESIZER PROJECT Building low-cost DNA synthesizers Our project has multiple phases, but the most interesting phase is the microfluidic chip. Microscopic channels guide the direction of fluids and reagents. Version 1: phosphoramidite chemistry. Version “the next one”: ligation chemistry from on- chip 4,096 droplet library (synthesis by shortoligo
GROWTH AND CHARACTERIZATION OF (110) INAS QUANTUM WELL Growth and Characterization of (110) InAs Quantum Well Heterostructures by Transmission Electron Microscopy and Electron Channeling Contrast Imaging. CLINICAL DEVELOPMENT SUCCESS RATES FOR INVESTIGATIONAL DRUGS 42 volume 32 NumBeR 1 JANuARY 2014 nature biotechnology data to annotate drugs by their biochemi-cal composition (e.g., peptide, nucleic acid, monoclonal antibody (mAb)) and molecu-lar size (i.e., large and small molecules). ADVANTAGES OF BEAM DECELERATION FOR LOW KV EDS ANALYSIS. Figure 1. EDS map of Slag at 3kV and 90nA: (a,c,e) No Gentle Beam; (b,d,f) -5kV Gentle Beam. a b e f c d a Microsc. Microanal. 21 (Suppl3), 2015 682
A PATHETICALLY SIMPLE CHARGE SENSITIVE AMPLIFIER A Pathetically Simple Charge Sensitive Amplifier The schematic above shows an extremely simple charge sensitive amplifier with only twoactive components.
HIGH SPEED LOGIC CIRCUITS DR. LYNN FULLER November 5, 2014 Dr. Lynn Fuller High Speed Logic Page 3 Rochester Institute of Technology Microelectronic Engineering OUTLINEIntroduction
EISENLAB - DIYHPL.US Maple Tree is a java-based, open source, cross-platform visualization tool to graphically browse the results of clustering analyses from our Cluster and Fuzzy K clustering software, and many other clustering and analysis programs. Maple Tree was developed by Lisa Simirenko in our lab. Combined Expression Data and Sequence Analysis. PHOTOINITIATORS FOR UVCURING 200 220 240 260 280 300 320 340 1.2 1.0 0.8 0.6 0.4 0.2 0.0 Key products, surface cure Absorption Spectra 0.001% (Acetonitrile) Wavelength (nm) Absorbance IRGACURE 184 DAROCUR 1173 IRGACURE 754 IRGACURE 2959 200 220 240 260 280 300 320 340 360 380PCR TROUBLESHOOTING
Increase primer amount. Increase template amount. Decrease annealing temperature by 6-10º C and check if you get any product. If you don't, check all your PCR ingredients. If you do get products (including unspecific ones) reaction conditions as described above. Combine some/all of the above. 4. YEAST CELL CYCLE FLOW CYTOMETRY PROTOCOL Protocol. Spin down 10 7 cells from an exponentially growing culture - 2000 rpm for 5 mins. Pour off supernatant. Vortex tube while adding 1.0 ml cold 70% EtOH. Store at 4 °C (cells keep ~indefinitely). When you want to process the cells, take 0.3 ml (this will be 2-3 x 10 6 cells, assuming a little loss in the washing) and add to 3 ml 50 mM LINUXCNC - LINUXCNC - ENHANCED MACHINE CONTROLLER (EMC) linuxcnc - enhanced machine controller (EMC) root: summary refs logtree commit diff
BITCOIN-WIZARDS
bitcoin-wizards wiki: root: summary refs log tree commit diff PORTLAB - BIOHACKING WIKI FOR PORTLAND, OREGON. Biohacking wiki for Portland, Oregon. root: summary refs log treecommit diff
PINY-COMMANDS
CGI forms for Piny: root: summary refs log tree commit diff UCAM - INTERACTIVE CONTROL OF DIY MICROSCOPES WITH interactive control of diy microscopes with multitouch support: root: summary refs log tree commit diff MOREWINKS - MAN I JUST CAN'T GET ENOUGH WINKS man i just can't get enough winks: root: summary refs log tree commitdiff
BASIC LABORATORY PRACTICES Basic Laboratory Practices. General Method for Cleavage of His-Tagged Proteins with Thrombin cleavage sites . Thrombin recognises the consensus sequence Leu-Val-Pro-Arg-Gly-Ser, cleaving the peptide bond between Arg and Gly. This is utilised in many vector systems which encode such a protease cleavage site allowing removal of an upstreamdomain.
PLANT ELECTROPHYSIOLOGY Preface Plant electrophysiology is the study of the electrochemical phenomena associated with biological cells and tissues in plants. It involves measurements of electrical FIBROBLAST CELL SYSTEMS INSTRUCTIONS For proliferating cells - Swab down the flask of proliferating cells with 70% ethanol or isopropanol, then place the flask in 37·C, 5% CO2, humidified incubator and allow to equilibrate for three to four hours. After cells have equilibrated, remove shipping medium from the flask following instructions on page 20. POLYETHYLENE GLYCOL-COATED BIOCOMPATIBLE SURFACES Polyethylene glycol–coated biocompatible surfaces Norma A. Alcantar, Eray S. Aydil, Jacob N. Israelachvili Chemical Engineering Department and Materials Department, University of California, Santa Barbara,California 93106
EDUCATION GUIDE SPECIAL STAINS 1 Introduction to Special Stains Sonja Wulff Thousands of years ago, diagnosing and treating a patient’s condition was much simpler. Ancient Egyptians blamed disease on demons, Greeks and Romans pointed THMR-IP5680 HP I-LINE PHOTORESIST 4 26-WE4C03-00 OHKA AMERICA, INC. 300 400 500 600 700 800 900 300 400 500 600 700 800 900 Mask CD (nm) Print CD (nm) THMR-iP5680 HP Target CD = 0.6 µm, exposure dose = 160 ms SPIN COATING: ONEDIMENSIONAL MODEL Spin coating: Onedimensional model D. E. Bornside, C. W. Macosko, and L. E. Scriven Citation: Journal of Applied Physics 66, 5185 (1989); doi: 10.1063/1.343754 GROWTH AND CHARACTERIZATION OF (110) INAS QUANTUM WELL Growth and Characterization of (110) InAs Quantum Well Heterostructures by Transmission Electron Microscopy and Electron Channeling Contrast Imaging. ADVANTAGES OF BEAM DECELERATION FOR LOW KV EDS ANALYSIS. Figure 1. EDS map of Slag at 3kV and 90nA: (a,c,e) No Gentle Beam; (b,d,f) -5kV Gentle Beam. a b e f c d a Microsc. Microanal. 21 (Suppl3), 2015 682
DAROCUR 1173
Ciba Specialty Chemicals Coating Effects Segment Ciba® DAROCUR® 1173 Edition: 4.9.2001, Basle Ciba Specialty Chemicals Inc. Page 1 of 3 General DAROCUR 1173 is a versatile highly efficient liquid photoinitiator which is used to initiate the photopolymerisation of chemically unsaturated BASIC LABORATORY PRACTICES Basic Laboratory Practices. General Method for Cleavage of His-Tagged Proteins with Thrombin cleavage sites . Thrombin recognises the consensus sequence Leu-Val-Pro-Arg-Gly-Ser, cleaving the peptide bond between Arg and Gly. This is utilised in many vector systems which encode such a protease cleavage site allowing removal of an upstreamdomain.
PLANT ELECTROPHYSIOLOGY Preface Plant electrophysiology is the study of the electrochemical phenomena associated with biological cells and tissues in plants. It involves measurements of electrical FIBROBLAST CELL SYSTEMS INSTRUCTIONS For proliferating cells - Swab down the flask of proliferating cells with 70% ethanol or isopropanol, then place the flask in 37·C, 5% CO2, humidified incubator and allow to equilibrate for three to four hours. After cells have equilibrated, remove shipping medium from the flask following instructions on page 20. POLYETHYLENE GLYCOL-COATED BIOCOMPATIBLE SURFACES Polyethylene glycol–coated biocompatible surfaces Norma A. Alcantar, Eray S. Aydil, Jacob N. Israelachvili Chemical Engineering Department and Materials Department, University of California, Santa Barbara,California 93106
EDUCATION GUIDE SPECIAL STAINS 1 Introduction to Special Stains Sonja Wulff Thousands of years ago, diagnosing and treating a patient’s condition was much simpler. Ancient Egyptians blamed disease on demons, Greeks and Romans pointed THMR-IP5680 HP I-LINE PHOTORESIST 4 26-WE4C03-00 OHKA AMERICA, INC. 300 400 500 600 700 800 900 300 400 500 600 700 800 900 Mask CD (nm) Print CD (nm) THMR-iP5680 HP Target CD = 0.6 µm, exposure dose = 160 ms SPIN COATING: ONEDIMENSIONAL MODEL Spin coating: Onedimensional model D. E. Bornside, C. W. Macosko, and L. E. Scriven Citation: Journal of Applied Physics 66, 5185 (1989); doi: 10.1063/1.343754 GROWTH AND CHARACTERIZATION OF (110) INAS QUANTUM WELL Growth and Characterization of (110) InAs Quantum Well Heterostructures by Transmission Electron Microscopy and Electron Channeling Contrast Imaging. ADVANTAGES OF BEAM DECELERATION FOR LOW KV EDS ANALYSIS. Figure 1. EDS map of Slag at 3kV and 90nA: (a,c,e) No Gentle Beam; (b,d,f) -5kV Gentle Beam. a b e f c d a Microsc. Microanal. 21 (Suppl3), 2015 682
DAROCUR 1173
Ciba Specialty Chemicals Coating Effects Segment Ciba® DAROCUR® 1173 Edition: 4.9.2001, Basle Ciba Specialty Chemicals Inc. Page 1 of 3 General DAROCUR 1173 is a versatile highly efficient liquid photoinitiator which is used to initiate the photopolymerisation of chemically unsaturated PLANT ELECTROPHYSIOLOGY Preface Plant electrophysiology is the study of the electrochemical phenomena associated with biological cells and tissues in plants. It involves measurements of electrical FIBROBLAST CELL SYSTEMS INSTRUCTIONS For proliferating cells - Swab down the flask of proliferating cells with 70% ethanol or isopropanol, then place the flask in 37·C, 5% CO2, humidified incubator and allow to equilibrate for three to four hours. After cells have equilibrated, remove shipping medium from the flask following instructions on page 20. PORTLAB - BIOHACKING WIKI FOR PORTLAND, OREGON. Biohacking wiki for Portland, Oregon. root: summary refs log treecommit diff
LINUXCNC - LINUXCNC - ENHANCED MACHINE CONTROLLER (EMC) linuxcnc - enhanced machine controller (EMC) root: summary refs logtree commit diff
PARAMETERIZED-MENDEL parameterized openscad models of 3d printable parts of reprap mendel: root: summary refs log tree commit diffPINY-COMMANDS
CGI forms for Piny: root: summary refs log tree commit diffBITCOIN-WIZARDS
bitcoin-wizards wiki: root: summary refs log tree commit diff UCAM - INTERACTIVE CONTROL OF DIY MICROSCOPES WITH interactive control of diy microscopes with multitouch support: root: summary refs log tree commit diffOPEN-SPECTROMETER
design files for a low-cost open source photospectrometer: root: summary refs log tree commit diff MOREWINKS - MAN I JUST CAN'T GET ENOUGH WINKS man i just can't get enough winks: root: summary refs log tree commitdiff
diyhpluswiki
* Edit
* RecentChanges
* History
* Preferences
ABOUT THIS WIKI
This is a wiki for open source hardware, do-it-yourself biohacking and practical transhumanism. The engineering teams behind this wiki can be found in the hplusroadmap IRC channel (logs ). Projects are self-funded by the group.EDITING THIS WIKI
Submit a pull request on github: https://github.com/kanzure/diyhpluswiki Register to edit here and see other commands . You can also clone this wiki with git and push changes back: git clone git://diyhpl.us/diyhpluswiki.git Note to push changes you must push with your registered account: git push username@diyhpl.us:/srv/git/diyhpluswiki.git master You can make this easier by updating the remotes: git remote set-url origin username@diyhpl.us:/srv/git/diyhpluswiki.git Now "git push" should work without debilitating complaint. browse wiki history on cgitINTERESTING PAGES
* genetic-modifications - proposed humanupgrades
* gene-editing - various gene therapy tools andmechanisms
* faq - various data and information on proteins * protein-engineering - protein folding and protein engineering wishlist * transcripts - bryan goes to conferences and types really really fast * hplusroadmap - wtf is hplusroadmap * projects - what can you do with DNA synthesis? * faq - this is a basic FAQ on do-it-yourselfbiohacking
* groups - stalk everyone in diybio for fun andprofit!
INDEX OF PAGES
* articles
* bitcoin
* cad
* chatsummaries
* diybio
* dna
* fda
* gene-editing
* gene-therapy
* genetic-modifications* goals.yaml
* homecmos
* hplusroadmap
* human-modifications.csv* index
* longevity
* myostatin
* nanotech
* nootropics
* polymerase
* projects
* protein-engineering* proteins
* scaffolding
* self-replicating machines* senses
* talk:diybio
* trans-tech.yaml
* transcripts
* transhumanism
* users
* wikiicons
* wikis
OTHER
Also there's a laser_etcher for microfluidicsproject (see cgit
).
Last edited Tue Feb 25 07:55:47 2020Details
Copyright © 2024 ArchiveBay.com. All rights reserved. Terms of Use | Privacy Policy | DMCA | 2021 | Feedback | Advertising | RSS 2.0