Are you over 18 and want to see adult content?
More Annotations
A complete backup of https://oneamerica.com
Are you over 18 and want to see adult content?
A complete backup of https://jackierupp.com
Are you over 18 and want to see adult content?
A complete backup of https://balcovalisesi.com
Are you over 18 and want to see adult content?
A complete backup of https://giftsaustralia.com.au
Are you over 18 and want to see adult content?
A complete backup of https://flytrippers.com
Are you over 18 and want to see adult content?
A complete backup of https://fortuneweek.com
Are you over 18 and want to see adult content?
A complete backup of https://cityoflakecharles.com
Are you over 18 and want to see adult content?
A complete backup of https://crypticmit.com
Are you over 18 and want to see adult content?
A complete backup of https://rcsmm.eu
Are you over 18 and want to see adult content?
A complete backup of https://lightfootforchicago.com
Are you over 18 and want to see adult content?
A complete backup of https://endhomelessness.org
Are you over 18 and want to see adult content?
Favourite Annotations
A complete backup of ftw.usatoday.com/2020/02/how-to-watch-tottenham-hotspur-vs-rb-leipzig-uefa-champions-league-live-stream-sch
Are you over 18 and want to see adult content?
A complete backup of stars.udn.com/star/story/10088/4356453
Are you over 18 and want to see adult content?
A complete backup of 24tv.ua/health/ru/jevakuacija_ukraincev_iz_uhanja_v_novyh_sanzharah_blokirujut_dorogi_tuda_prignali_voennuj
Are you over 18 and want to see adult content?
Text
screening.
EGFR C.2236 2250DEL15 P.E746 A750DELELREA PROTOCOL 3 1.5. Incubate the culture at 37°C and 5% CO 2. 2. Passaging procedure 2.1. Remove and discard culture medium. 2.2. Briefly rinse the cell layer with sterile, 1x PBS, or 0.25% (w/v) Trypsin-0.53% (w/v) EDTA solution in order to HUMAN INDUCED PLURIPOTENT STEM CELLS (FROM NORMAL, MALE Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US Toll Free); 408-773-8007 Fax:408-773-8238
WWW.APPLIEDSTEMCELL.COM www.appliedstemcell.comDATASHEET
Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US Toll Free); 408-773-8007 Fax:408-773-8238
EGFR C.2240 2257DEL18; P.L747 P753>S Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US DATASHEET - APPLIEDSTEMCELL.COM Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US EGFR C.2319 2320INSCAC; P.H773 V774INSH Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US CHO-S MASTER CELL LINE & KNOCKIN KIT Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US Toll Free); 408-773-8007 Fax:408-773-8238
MAMMALIAN CELL DISPLAY SYSTEM: CONSTRUCTION OF A NAÏVE Mammalian Cell Display System: Construction of a Naïve Library by Insertion of Human Immunoglobulin Germline Sequences Using TARGATT™Technology Xiuling Chi*, Alfonso Farruggio, Iris PRECLINICAL CRO, STEM CELL, ANIMAL MODELS WITH CRISPR HEK293/CHO cells for bioproduction; humanized mouse models for functional validation. Technology platform to drive discovery and screening of antibodies/biotherapeutics. Customized solutions for your R&D projects: custom co-cultures for physiologically relevant modeling, large-scale cell line expansion for biobanking, and 3Dscreening.
EGFR C.2236 2250DEL15 P.E746 A750DELELREA PROTOCOL 3 1.5. Incubate the culture at 37°C and 5% CO 2. 2. Passaging procedure 2.1. Remove and discard culture medium. 2.2. Briefly rinse the cell layer with sterile, 1x PBS, or 0.25% (w/v) Trypsin-0.53% (w/v) EDTA solution in order to HUMAN INDUCED PLURIPOTENT STEM CELLS (FROM NORMAL, MALE Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US Toll Free); 408-773-8007 Fax:408-773-8238
WWW.APPLIEDSTEMCELL.COM www.appliedstemcell.comDATASHEET
Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US Toll Free); 408-773-8007 Fax:408-773-8238
EGFR C.2240 2257DEL18; P.L747 P753>S Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US DATASHEET - APPLIEDSTEMCELL.COM Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US EGFR C.2319 2320INSCAC; P.H773 V774INSH Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US CHO-S MASTER CELL LINE & KNOCKIN KIT Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US Toll Free); 408-773-8007 Fax:408-773-8238
MAMMALIAN CELL DISPLAY SYSTEM: CONSTRUCTION OF A NAÏVE Mammalian Cell Display System: Construction of a Naïve Library by Insertion of Human Immunoglobulin Germline Sequences Using TARGATT™Technology Xiuling Chi*, Alfonso Farruggio, Iris WWW.APPLIEDSTEMCELL.COM www.appliedstemcell.com HUMAN INDUCED PLURIPOTENT STEM CELL (HIPSC) MASTER CELL PROTOCOL 3 Schematic Representation of TARGATT™ Knockin Strategy Figure 1. Schematic representation of TARGATT™ site-specific transgene integration mediated by integrase. The TARGATT™ hiPSC Master Cell Line was engineered with an CHO-S MASTER CELL LINE & KNOCKIN KIT Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US Toll Free); 408-773-8007 Fax:408-773-8238
EGFR C.2319 2320INSCAC; P.H773 V774INSH Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US DATASHEET - APPLIED STEMCELL INC Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US Toll Free); 408-773-8007 Fax:408-773-8238
SAFE HARBOR LOCUS HROSA26 KNOCK-IN KIT FOR HUMAN CELL LINE Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US ONCO-REFª SINGLE MUTATION REFERENCE STANDARD SERIES AccuREFAccuref Diagnostics Diagnostics Advancing Personalized Medicine Through Precision Genome Engineering A Division of Applied StemCell, Inc. 521 Cottonwood Drive, Suite 111 Milpitas, CA 95035 (SF Bay Area) info@accuref.com Phone 1.408.773.8007 (PST)info@accuref.com DR4 MEF CELLS, P3, MITOMYCIN-C-TREATED (DR4 MOUSE Title: Microsoft Word - ASF-1023-24-25-26-DR4-MEF-P3-20170109.docx Created Date: 1/10/2017 10:14:47 PM EZ-IPSC GENERATION KIT PROTOCOL 5 1.5.16 Resuspend the cells in 2 mL of Fibroblast Medium. Count the cells. Seed 1 x 10^4, 2 x 10^4, and 4 x 10^4 transfected fibroblasts, respectively, in the wells of a 6-well plate pre-coatedMatrigel.
EGFR C.2310 2311INSGGT; P.D770 N771INSG PROTOCOL 3 2.2. Briefly rinse the cell layer with sterile, 1x PBS, or 0.25% (w/v) Trypsin-0.53% (w/v) EDTA solution in order to remove all traces of serum, which can inhibit trypsin activity. PRECLINICAL CRO, STEM CELL, ANIMAL MODELS WITH CRISPR HEK293/CHO cells for bioproduction; humanized mouse models for functional validation. Technology platform to drive discovery and screening of antibodies/biotherapeutics. Customized solutions for your R&D projects: custom co-cultures for physiologically relevant modeling, large-scale cell line expansion for biobanking, and 3Dscreening.
EGFR C.2236 2250DEL15 P.E746 A750DELELREA PROTOCOL 3 1.5. Incubate the culture at 37°C and 5% CO 2. 2. Passaging procedure 2.1. Remove and discard culture medium. 2.2. Briefly rinse the cell layer with sterile, 1x PBS, or 0.25% (w/v) Trypsin-0.53% (w/v) EDTA solution in order to HUMAN INDUCED PLURIPOTENT STEM CELLS (FROM NORMAL, MALE Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US Toll Free); 408-773-8007 Fax:408-773-8238
WWW.APPLIEDSTEMCELL.COM www.appliedstemcell.comDATASHEET
Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US Toll Free); 408-773-8007 Fax:408-773-8238
EGFR C.2240 2257DEL18; P.L747 P753>S Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US DATASHEET - APPLIEDSTEMCELL.COM Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US EGFR C.2319 2320INSCAC; P.H773 V774INSH Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US CHO-S MASTER CELL LINE & KNOCKIN KIT Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US Toll Free); 408-773-8007 Fax:408-773-8238
MAMMALIAN CELL DISPLAY SYSTEM: CONSTRUCTION OF A NAÏVE Mammalian Cell Display System: Construction of a Naïve Library by Insertion of Human Immunoglobulin Germline Sequences Using TARGATT™Technology Xiuling Chi*, Alfonso Farruggio, Iris PRECLINICAL CRO, STEM CELL, ANIMAL MODELS WITH CRISPR HEK293/CHO cells for bioproduction; humanized mouse models for functional validation. Technology platform to drive discovery and screening of antibodies/biotherapeutics. Customized solutions for your R&D projects: custom co-cultures for physiologically relevant modeling, large-scale cell line expansion for biobanking, and 3Dscreening.
EGFR C.2236 2250DEL15 P.E746 A750DELELREA PROTOCOL 3 1.5. Incubate the culture at 37°C and 5% CO 2. 2. Passaging procedure 2.1. Remove and discard culture medium. 2.2. Briefly rinse the cell layer with sterile, 1x PBS, or 0.25% (w/v) Trypsin-0.53% (w/v) EDTA solution in order to HUMAN INDUCED PLURIPOTENT STEM CELLS (FROM NORMAL, MALE Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US Toll Free); 408-773-8007 Fax:408-773-8238
WWW.APPLIEDSTEMCELL.COM www.appliedstemcell.comDATASHEET
Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US Toll Free); 408-773-8007 Fax:408-773-8238
EGFR C.2240 2257DEL18; P.L747 P753>S Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US DATASHEET - APPLIEDSTEMCELL.COM Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US EGFR C.2319 2320INSCAC; P.H773 V774INSH Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US CHO-S MASTER CELL LINE & KNOCKIN KIT Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US Toll Free); 408-773-8007 Fax:408-773-8238
MAMMALIAN CELL DISPLAY SYSTEM: CONSTRUCTION OF A NAÏVE Mammalian Cell Display System: Construction of a Naïve Library by Insertion of Human Immunoglobulin Germline Sequences Using TARGATT™Technology Xiuling Chi*, Alfonso Farruggio, Iris WWW.APPLIEDSTEMCELL.COM www.appliedstemcell.com CHO-S MASTER CELL LINE & KNOCKIN KIT Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US Toll Free); 408-773-8007 Fax:408-773-8238
EGFR C.2319 2320INSCAC; P.H773 V774INSH Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US DATASHEET - APPLIED STEMCELL INC Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US Toll Free); 408-773-8007 Fax:408-773-8238
SAFE HARBOR LOCUS HROSA26 KNOCK-IN KIT FOR HUMAN CELL LINE Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US EZ-IPSC GENERATION KIT PROTOCOL 5 1.5.16 Resuspend the cells in 2 mL of Fibroblast Medium. Count the cells. Seed 1 x 10^4, 2 x 10^4, and 4 x 10^4 transfected fibroblasts, respectively, in the wells of a 6-well plate pre-coatedMatrigel.
DR4 MEF CELLS, P3, MITOMYCIN-C-TREATED (DR4 MOUSE Title: Microsoft Word - ASF-1023-24-25-26-DR4-MEF-P3-20170109.docx Created Date: 1/10/2017 10:14:47 PM ONCO-REFª SINGLE MUTATION REFERENCE STANDARD SERIES AccuREFAccuref Diagnostics Diagnostics Advancing Personalized Medicine Through Precision Genome Engineering A Division of Applied StemCell, Inc. 521 Cottonwood Drive, Suite 111 Milpitas, CA 95035 (SF Bay Area) info@accuref.com Phone 1.408.773.8007 (PST)info@accuref.com EGFR C.2310 2311INSGGT; P.D770 N771INSG PROTOCOL 3 2.2. Briefly rinse the cell layer with sterile, 1x PBS, or 0.25% (w/v) Trypsin-0.53% (w/v) EDTA solution in order to remove all traces of serum, which can inhibit trypsin activity. SAFE HARBOR KNOCK-IN IPSCS: AAVS1-CAG-GFP CHR19 IPSC PROTOCOL 3 Karyotype Analysis Figure 2. Karyotype analysis of AAVS1-CAG-GFP chr19 iPSC line indicates normal karyotype and no clonal abnormalities in the iPSC line at the stated band level of resolution, after genome editing. PRECLINICAL CRO, STEM CELL, ANIMAL MODELS WITH CRISPR HEK293/CHO cells for bioproduction; humanized mouse models for functional validation. Technology platform to drive discovery and screening of antibodies/biotherapeutics. Customized solutions for your R&D projects: custom co-cultures for physiologically relevant modeling, large-scale cell line expansion for biobanking, and 3Dscreening.
EGFR C.2236 2250DEL15 P.E746 A750DELELREA PROTOCOL 3 1.5. Incubate the culture at 37°C and 5% CO 2. 2. Passaging procedure 2.1. Remove and discard culture medium. 2.2. Briefly rinse the cell layer with sterile, 1x PBS, or 0.25% (w/v) Trypsin-0.53% (w/v) EDTA solution in order to HUMAN INDUCED PLURIPOTENT STEM CELLS (FROM NORMAL, MALEAPPLIED STEMCELL INCSTEM CELL INCAPPLIED STEM CELLS COMPANYSTEM CELL RESEARCH JOBSSTEM CELL SERVICESSTEM CELL STOCK Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US Toll Free); 408-773-8007 Fax:408-773-8238
DATASHEET
Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US Toll Free); 408-773-8007 Fax:408-773-8238
DATASHEET - APPLIEDSTEMCELL.COM Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US EGFR C.2319 2320INSCAC; P.H773 V774INSH Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US CHO-S MASTER CELL LINE & KNOCKIN KITCHO STABLE CELL LINECHO STABLE CELL LINE DEVELOPMENTTARGET CELL PHONESTARGET CELL PHONES CONSUMER CELLULARTARGET CELL PHONES NO CONTRACTTYPES OF CELL LINES Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US Toll Free); 408-773-8007 Fax:408-773-8238
MOUSE EMBRYONIC STEM CELL (C57BL/6-EZ) PROTOCOL 2 Cryopreservation Frozen in 1 mL freezing medium (90% FBS and 10% DMSO) Shipping Dry ice Storage and Stability Store in liquid nitrogen freezer immediately upon receipt. This product is stable for at least 6 months from the date of receiving when stored as directed. EZ-IPSC GENERATION KIT PROTOCOL 5 1.5.16 Resuspend the cells in 2 mL of Fibroblast Medium. Count the cells. Seed 1 x 10^4, 2 x 10^4, and 4 x 10^4 transfected fibroblasts, respectively, in the wells of a 6-well plate pre-coatedMatrigel.
DR4 MEF CELLS, P3, MITOMYCIN-C-TREATED (DR4 MOUSE Title: Microsoft Word - ASF-1023-24-25-26-DR4-MEF-P3-20170109.docx Created Date: 1/10/2017 10:14:47 PM PRECLINICAL CRO, STEM CELL, ANIMAL MODELS WITH CRISPR HEK293/CHO cells for bioproduction; humanized mouse models for functional validation. Technology platform to drive discovery and screening of antibodies/biotherapeutics. Customized solutions for your R&D projects: custom co-cultures for physiologically relevant modeling, large-scale cell line expansion for biobanking, and 3Dscreening.
EGFR C.2236 2250DEL15 P.E746 A750DELELREA PROTOCOL 3 1.5. Incubate the culture at 37°C and 5% CO 2. 2. Passaging procedure 2.1. Remove and discard culture medium. 2.2. Briefly rinse the cell layer with sterile, 1x PBS, or 0.25% (w/v) Trypsin-0.53% (w/v) EDTA solution in order to HUMAN INDUCED PLURIPOTENT STEM CELLS (FROM NORMAL, MALEAPPLIED STEMCELL INCSTEM CELL INCAPPLIED STEM CELLS COMPANYSTEM CELL RESEARCH JOBSSTEM CELL SERVICESSTEM CELL STOCK Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US Toll Free); 408-773-8007 Fax:408-773-8238
DATASHEET
Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US Toll Free); 408-773-8007 Fax:408-773-8238
DATASHEET - APPLIEDSTEMCELL.COM Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US EGFR C.2319 2320INSCAC; P.H773 V774INSH Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US CHO-S MASTER CELL LINE & KNOCKIN KITCHO STABLE CELL LINECHO STABLE CELL LINE DEVELOPMENTTARGET CELL PHONESTARGET CELL PHONES CONSUMER CELLULARTARGET CELL PHONES NO CONTRACTTYPES OF CELL LINES Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US Toll Free); 408-773-8007 Fax:408-773-8238
MOUSE EMBRYONIC STEM CELL (C57BL/6-EZ) PROTOCOL 2 Cryopreservation Frozen in 1 mL freezing medium (90% FBS and 10% DMSO) Shipping Dry ice Storage and Stability Store in liquid nitrogen freezer immediately upon receipt. This product is stable for at least 6 months from the date of receiving when stored as directed. EZ-IPSC GENERATION KIT PROTOCOL 5 1.5.16 Resuspend the cells in 2 mL of Fibroblast Medium. Count the cells. Seed 1 x 10^4, 2 x 10^4, and 4 x 10^4 transfected fibroblasts, respectively, in the wells of a 6-well plate pre-coatedMatrigel.
DR4 MEF CELLS, P3, MITOMYCIN-C-TREATED (DR4 MOUSE Title: Microsoft Word - ASF-1023-24-25-26-DR4-MEF-P3-20170109.docx Created Date: 1/10/2017 10:14:47 PM CHO-S MASTER CELL LINE & KNOCKIN KIT Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US Toll Free); 408-773-8007 Fax:408-773-8238
DATASHEET
PROTOCOL 3 Media and Material Catalog# Component Amount AST-1305-1 TARGATT™ HEK293 Master Cell Line 1 x 10^6 cells AST-3064 TARGATT™ 24 CMV-MCS-attB; Cloning Plasmid*# 5 µg AST-3065 15TARGATT™ 25 mCherry-attB (No Promoter); Positive Control Plasmid# µg AST-3201 TARGATT™ CMV-integrase Plasmid# 15 µg * Amplify the cloning plasmid to clone your gene of DATASHEET - APPLIEDSTEMCELL.COM Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US Toll Free); 408-773-8007 Fax:408-773-8238
IPSC DERIVED T CELLS HPCS Primary Cells (From Normal, Male, Human Fibroblast) iPS Cells (ASE-9211) Reprogramming (Episomal) 2-3 weeks Di˜erentiation: iPSC-Derived Hematopoietic Progenitor CellsNRAS C.182A>G P
Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US Toll Free); 408-773-8007 Fax:408-773-8238
EGFR C.2240 2257DEL18; P.L747 P753>S Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US TM SERUM-AND FEEDER-FREE MEDIUM(SFFM %%PROTOCOL’ % 2% Size 100 mL Application Feeder/Serum free hESC/iPSC culture Source Chemical plus growth factors Storage -80˚C Shipping Shipped on dry ice. Restricted Use This product is for research use only and not intended for human or animal diagnostic, daysinculture’ SFFMvs.’mTeSRComparison’Using’iPSCs:’Passage’#5’ BRAF C.1799 1800TG>AT; P. V600D Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US PRECLINICAL CRO, STEM CELL, ANIMAL MODELS WITH CRISPR Author: makiko Created Date: 12/28/2016 10:18:52 AM ONCO-REFª SINGLE MUTATION REFERENCE STANDARD SERIES An 18 bp deletion in exon 19 of EGFR, corresponding to COSMIC mutation 6255 (c.2239_2256del18), was engineered into the RKO cell line. PRECLINICAL CRO, STEM CELL, ANIMAL MODELS WITH CRISPR HEK293/CHO cells for bioproduction; humanized mouse models for functional validation. Technology platform to drive discovery and screening of antibodies/biotherapeutics. Customized solutions for your R&D projects: custom co-cultures for physiologically relevant modeling, large-scale cell line expansion for biobanking, and 3Dscreening.
EGFR C.2236 2250DEL15 P.E746 A750DELELREA PROTOCOL 3 1.5. Incubate the culture at 37°C and 5% CO 2. 2. Passaging procedure 2.1. Remove and discard culture medium. 2.2. Briefly rinse the cell layer with sterile, 1x PBS, or 0.25% (w/v) Trypsin-0.53% (w/v) EDTA solution in order to HUMAN INDUCED PLURIPOTENT STEM CELLS (FROM NORMAL, MALEAPPLIED STEMCELL INCSTEM CELL INCAPPLIED STEM CELLS COMPANYSTEM CELL RESEARCH JOBSSTEM CELL SERVICESSTEM CELL STOCK Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US Toll Free); 408-773-8007 Fax:408-773-8238
DATASHEET
Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US Toll Free); 408-773-8007 Fax:408-773-8238
DATASHEET - APPLIEDSTEMCELL.COM Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US EGFR C.2319 2320INSCAC; P.H773 V774INSH Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US CHO-S MASTER CELL LINE & KNOCKIN KITCHO STABLE CELL LINECHO STABLE CELL LINE DEVELOPMENTTARGET CELL PHONESTARGET CELL PHONES CONSUMER CELLULARTARGET CELL PHONES NO CONTRACTTYPES OF CELL LINES Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US Toll Free); 408-773-8007 Fax:408-773-8238
MOUSE EMBRYONIC STEM CELL (C57BL/6-EZ) PROTOCOL 2 Cryopreservation Frozen in 1 mL freezing medium (90% FBS and 10% DMSO) Shipping Dry ice Storage and Stability Store in liquid nitrogen freezer immediately upon receipt. This product is stable for at least 6 months from the date of receiving when stored as directed. EZ-IPSC GENERATION KIT PROTOCOL 5 1.5.16 Resuspend the cells in 2 mL of Fibroblast Medium. Count the cells. Seed 1 x 10^4, 2 x 10^4, and 4 x 10^4 transfected fibroblasts, respectively, in the wells of a 6-well plate pre-coatedMatrigel.
DR4 MEF CELLS, P3, MITOMYCIN-C-TREATED (DR4 MOUSE Title: Microsoft Word - ASF-1023-24-25-26-DR4-MEF-P3-20170109.docx Created Date: 1/10/2017 10:14:47 PM PRECLINICAL CRO, STEM CELL, ANIMAL MODELS WITH CRISPR HEK293/CHO cells for bioproduction; humanized mouse models for functional validation. Technology platform to drive discovery and screening of antibodies/biotherapeutics. Customized solutions for your R&D projects: custom co-cultures for physiologically relevant modeling, large-scale cell line expansion for biobanking, and 3Dscreening.
EGFR C.2236 2250DEL15 P.E746 A750DELELREA PROTOCOL 3 1.5. Incubate the culture at 37°C and 5% CO 2. 2. Passaging procedure 2.1. Remove and discard culture medium. 2.2. Briefly rinse the cell layer with sterile, 1x PBS, or 0.25% (w/v) Trypsin-0.53% (w/v) EDTA solution in order to HUMAN INDUCED PLURIPOTENT STEM CELLS (FROM NORMAL, MALEAPPLIED STEMCELL INCSTEM CELL INCAPPLIED STEM CELLS COMPANYSTEM CELL RESEARCH JOBSSTEM CELL SERVICESSTEM CELL STOCK Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US Toll Free); 408-773-8007 Fax:408-773-8238
DATASHEET
Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US Toll Free); 408-773-8007 Fax:408-773-8238
DATASHEET - APPLIEDSTEMCELL.COM Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US EGFR C.2319 2320INSCAC; P.H773 V774INSH Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US CHO-S MASTER CELL LINE & KNOCKIN KITCHO STABLE CELL LINECHO STABLE CELL LINE DEVELOPMENTTARGET CELL PHONESTARGET CELL PHONES CONSUMER CELLULARTARGET CELL PHONES NO CONTRACTTYPES OF CELL LINES Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US Toll Free); 408-773-8007 Fax:408-773-8238
MOUSE EMBRYONIC STEM CELL (C57BL/6-EZ) PROTOCOL 2 Cryopreservation Frozen in 1 mL freezing medium (90% FBS and 10% DMSO) Shipping Dry ice Storage and Stability Store in liquid nitrogen freezer immediately upon receipt. This product is stable for at least 6 months from the date of receiving when stored as directed. EZ-IPSC GENERATION KIT PROTOCOL 5 1.5.16 Resuspend the cells in 2 mL of Fibroblast Medium. Count the cells. Seed 1 x 10^4, 2 x 10^4, and 4 x 10^4 transfected fibroblasts, respectively, in the wells of a 6-well plate pre-coatedMatrigel.
DR4 MEF CELLS, P3, MITOMYCIN-C-TREATED (DR4 MOUSE Title: Microsoft Word - ASF-1023-24-25-26-DR4-MEF-P3-20170109.docx Created Date: 1/10/2017 10:14:47 PM CHO-S MASTER CELL LINE & KNOCKIN KIT Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US Toll Free); 408-773-8007 Fax:408-773-8238
DATASHEET
PROTOCOL 3 Media and Material Catalog# Component Amount AST-1305-1 TARGATT™ HEK293 Master Cell Line 1 x 10^6 cells AST-3064 TARGATT™ 24 CMV-MCS-attB; Cloning Plasmid*# 5 µg AST-3065 15TARGATT™ 25 mCherry-attB (No Promoter); Positive Control Plasmid# µg AST-3201 TARGATT™ CMV-integrase Plasmid# 15 µg * Amplify the cloning plasmid to clone your gene of DATASHEET - APPLIEDSTEMCELL.COM Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US Toll Free); 408-773-8007 Fax:408-773-8238
IPSC DERIVED T CELLS HPCS Primary Cells (From Normal, Male, Human Fibroblast) iPS Cells (ASE-9211) Reprogramming (Episomal) 2-3 weeks Di˜erentiation: iPSC-Derived Hematopoietic Progenitor CellsNRAS C.182A>G P
Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US Toll Free); 408-773-8007 Fax:408-773-8238
EGFR C.2240 2257DEL18; P.L747 P753>S Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US TM SERUM-AND FEEDER-FREE MEDIUM(SFFM %%PROTOCOL’ % 2% Size 100 mL Application Feeder/Serum free hESC/iPSC culture Source Chemical plus growth factors Storage -80˚C Shipping Shipped on dry ice. Restricted Use This product is for research use only and not intended for human or animal diagnostic, daysinculture’ SFFMvs.’mTeSRComparison’Using’iPSCs:’Passage’#5’ BRAF C.1799 1800TG>AT; P. V600D Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Applied StemCell, Inc. 521 Cottonwood Dr. #111, Milpitas, CA 95035 Phone: 866-497-4180 (US PRECLINICAL CRO, STEM CELL, ANIMAL MODELS WITH CRISPR Author: makiko Created Date: 12/28/2016 10:18:52 AM ONCO-REFª SINGLE MUTATION REFERENCE STANDARD SERIES An 18 bp deletion in exon 19 of EGFR, corresponding to COSMIC mutation 6255 (c.2239_2256del18), was engineered into the RKO cell line. JAVASCRIPT SEEMS TO BE DISABLED IN YOUR BROWSER. For the best experience on our site, be sure to turn on Javascript in your browser. Contact Us: By Phone 8am- 6pm PST 1-866-497-4180 (Toll Free) or 1-408-773-8007 By Email Contact Us By Fax 1-650-800-7179CURRENCY
USD - US DOLLAR
* EUR - Euro
Skip to Content
Toggle Nav
0 Items ($0.00)
MY CART 0
Close YOU HAVE NO ITEMS IN YOUR SHOPPING CART.* Welcome
* Sign In
* |
* Register
SEARCH
Search
Advanced Search
Search
Menu
* Services
* Stem Cell Services * Neurotoxicity Screening * CRISPR/Cas9 Genome Editing * TARGATT™ Genome Editing* Bioproduction
* Lentivirus Stable Cell Line Generation * Virus Packaging & DNA Cloning* CRO Services
* Products
* Stem Cell
* TARGATT™ Genome Editing * Genetically Modified Cell Lines / Isogenic Cell Lines * Cancer Research Tools* Sample Prep
* Animal Models
* Mouse Models
* Rat Models
* Custom In Vivo Assay Services* Cell Line Models
* Stem Cells - iPSC and ESCs* Cancer Research
* Antibody Validation * UNISELECT™ Antibody Discovery and Screening* Cell-Based Assays
* Cell-Based Drug Toxicity and Efficacy Testing * Bioproduction Services- High Yield CHO Cell Lines* Custom Services
* Ordering
* How to Order
* Distributors
* Support
* Supporting Materials * Poster / White Paper* Webinar
* Brochure / Flyer
* Promotion
* References
* Product Data Sheets* Useful Links
* Contact Form
* About Us
* Our Board
* Distributors
* News & Events
* Careers
* Partners
* Facility
* Contact Us
* Services
* Stem Cell Services * Neurotoxicity Screening * CRISPR/Cas9 Genome Editing * TARGATT™ Genome Editing* Bioproduction
* Lentivirus Stable Cell Line Generation * Virus Packaging & DNA Cloning* CRO Services
* Products
* Stem Cell
* TARGATT™ Genome Editing * Genetically Modified Cell Lines / Isogenic Cell Lines * Cancer Research Tools* Sample Prep
* Animal Models
* Mouse Models
* Rat Models
* Custom In Vivo Assay Services* Cell Line Models
* Stem Cells - iPSC and ESCs* Cancer Research
* Antibody Validation * UNISELECT™ Antibody Discovery and Screening* Cell-Based Assays
* Cell-Based Drug Toxicity and Efficacy Testing * Bioproduction Services- High Yield CHO Cell Lines* Custom Services
* Ordering
* How to Order
* Distributors
* Support
* Supporting Materials * Poster / White Paper* Webinar
* Brochure / Flyer
* Promotion
* References
* Product Data Sheets* Useful Links
* Contact Form
* About Us
* Our Board
* Distributors
* News & Events
* Careers
* Partners
* Facility
* Contact Us
Contact Us
Settings
CURRENCY
USD - US DOLLAR
* EUR - Euro
Close
*
Genome Editing &
Cell Therapy CRO
Cell line models, mouse models, preclinical studies proposals inand bioproduction.
* Previous
* Next
* 3000+ academic & industry clients * 11+ years' experience * NIST genome editing consortium memberHOME PAGE
*
Stem Cell Services
------------------------- Custom solutions for your entire stem cell research workflow: Biorelevant iPSC/ESC-based disease model generation to cell-based assays for characterization and drug screening. * Stem Cell Services * iPSC Generation from Patient Samples * Stem Cell Genome Editing * Neural Stem Cell Differentiation * Teratoma Analysis, iPSC Characterization*
Genome Editing
------------------------- Precision genome editing using CRISPR/Cas9 and TARGATT™ technologies and optimized multi-approach protocols for “next-generation” mouse, rat and cell line model generation. * CRISPR/Cas9 Genome Editing * TARGATT™ Genome Editing* Cell Line Models
* Mouse Models
* Rat Models
* Lentivirus Stable Cell Line Generation Service*
Mouse Models
------------------------- AAALAC-accredited provider of quality service and products for engineering mouse models with a wide range of genetic modifications using CRISPR/Cas9 and TARGATT™ technologies.* Mouse Models
* Knockout, Knock-in, Point Mutation Mouse Models * Conditional Knockout Mouse Models * Large Transgene Knock-in Mouse Models * Purchase TARGATT™ Mouse Model Products*
Neurotoxicity Testing ------------------------- Comprehensive cell-based test battery for screening preclinical drug candidates for neurotoxicity and CNS drug efficacy using fully characterized iPSC-differentiated neural lineage cells. * Neurotoxicity Testing * Cytotoxicity & Cell Viability Assays MTT/ MTS cell proliferationassay
* iPSC disease modeling - Toxicology Testing*
Cancer Research
------------------------- Custom services and high-quality products to support and accelerate cancer research: animal/cell line models, and comprehensive _in vitro_ and _in vivo_ assay solutions for basic research and drug discovery/screening.
* Cell Line Models
* Mouse Models
* Antibody Validation * Antibody Screening* Cell Based Assays
* Genetically Modified / Isogenic Cell Lines*
Bioproduction &
CRO Services
------------------------- Custom assay solutions to meet the needs of client’s _in vivo_ and _in vitro_ research: assay development, drug screening, and an efficient bioproduction platform to test high-yield competency ofbiomolecules.
* CHO Cells
* Cell Based Assays
* Animal (_In Vivo_) Services* Custom Services
Popular Products
Stem Cell
Research Products
ISO-quality products for all stages of stem cell applications. * Stem Cell Products * iPSC Differentiated Cells* iPSC & ESC Lines
* Gene Edited iPSC Lines* MEF Feeder Cells
TARGATT™
Genome Editing
Make your own “fast & site-specific” TARGATT™ transgenic mousemodels.
* TARGATT™ Mouse Model Generation * TARGATT™ Transgenic Kits * TARGATT™ Plasmids * TARGATT™ Knock-in Master Cell Line Genetically Modified Cell Lines / Isogenic Cell Lines Genetically engineered cell lines for cancer and neuroscienceresearch.
* Genetically Modified Cell Lines / Isogenic Cell Lines * Gene Edited iPSC Lines * Cell Line Model Generation Cancer Research Tools Advanced toolkits for cancer research: isogenic cell lines, quantitative tumorigenesis and more. * Cancer Research Tools * CRISPR/Cas9 Genome Editing * Substrate-free Autobiolumi nescent Cell Lines and Vectors _In Vivo_ services (Animal CRO services): Disease modeling and downstream assay solutions using animal models: ASC offers a fully customizable assay platform for research using animal models: animal model engineering, in vivo assessments, and postmortem analyses.Learn More
Have Questions?
An Applied StemCell technical expert is happy to help, contact ustoday!
Contact Us
NEWSLETTER
Sign up to receive technical advice and exclusive deals directly toyour inbox.
Sign Up
SERVICES
* Stem Cell Services * Neurotoxicity Screening * CRISPR/Cas9 Genome Editing * TARGATT™ Genome Editing* Bioproduction
* Lentivirus Stable Cell Line Generation * DNA Cloning & Virus Packaging* CRO Services
PRODUCTS
* Stem Cells (Induced Pluripotent Stem Cells, iPSCs) * TARGATT™ Genome Editing * Genetically Modified Cell Lines / Isogenic Cell Lines * Cancer Research Tools ANIMAL AND CELL LINE MODELS* Mouse Models
* Rat Models
* Stem Cells - iPSCs and ESCs* Cancer Cell Lines
* Cell Line IrradiationABOUT US
* Our Board
* Distributors
* News & Events
* Careers
* Partners
* Facility
ORDERING
* How to Order
* Distributors
SUPPORT
* Supporting Materials* Promotions
* References
* Product Data Sheets* Useful Links
* Contact Form
CONTACT US
Applied StemCell, Inc. 521 Cottonwood Dr. Suite #111 Milpitas, California 95035, USA__ 1-866-497-4180
__ 1-650-800-7179
------------------------- Copyright © 2018 Applied StemCell. All rights reserved.* Privacy Policy
* |
* Terms of Use
* |
* Sitemap
Close
CHECKOUT OUT AS A NEW CUSTOMER Creating an account has many benefits: * See order and shipping status * Track order history* Check out faster
Create Account
CHECKOUT OUT USING YOUR ACCOUNTEmail Address
Password
Sign In
Forgot Your Password?Leave us a message
Name *
Email *
Country *Select CountryUnited StatesAustraliaAustriaBelgiumCanadaChileChinaCzech RepublicDenmarkFinlandFranceGermanyGreeceHong KongHungaryIndiaIndonesiaIranIrelandIsraelItalyJapanSouth KoreaMexicoNetherlandsNew ZealandNorwayRussiaSingaporeSpainSwedenSwitzerlandTaiwanTurkeyUkraineUnited KingdomOther EUOther AsiaOthers State(Ony USA)Please select stateAlaskaAlabamaArkansasArizonaCaliforniaColoradoConnecticutDistrictOf
ColumbiaDelawareFloridaGeorgiaHawaiiIowaIdahoIllinoisIndianaKansasKentuckyLouisianaMassachusettsMarylandMaineMichiganMinnesotaMissouriMississippiMontanaNorth CarolinaNorth DakotaNebraskaNew HampshireNew JerseyNew MexicoNevadaNew YorkOhioOklahomaOregonPennsylvaniaRhode IslandSouth CarolinaSouth DakotaTennesseeTexasUtahVirginiaVermontWashingtonWisconsinWestVirginiaWyoming
Message *
Send
Details
Copyright © 2024 ArchiveBay.com. All rights reserved. Terms of Use | Privacy Policy | DMCA | 2021 | Feedback | Advertising | RSS 2.0