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PRODUCT DATA AND DOCUMENTS A Safety Data sheets (SDS) or Material Safety Data Sheet (MSDS) details the safety considerations for a particular substance. This includes the physical, health and environmental hazards, protective measures and safety precautions for handling, storing, and transporting that substance. Many SDS documents are available on theproduct's online
ANTI-COLLAGEN III ANTIBODY (AB7778) Anti-Collagen III antibody (ab184993) Research with confidence – consistent and reproducible results with every batch. Long-term and scalable supply – powered by recombinant technology for fast production. Success from the first experiment – confirmed specificity through extensive validation. Ethical standards compliant RECOMBINANT ANTI-BDNF ANTIBODY (AB108319) Immunohistochemistry (Frozen sections) - Anti-BDNF antibody (ab108319) Immunohistochemistry (Frozen sections) analysis of mouse cerebrum tissue sections labeling BDNF with Purified ab108319 at 1/100 (2.8 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). ANTI-ADENOVIRUS TYPE 5 ANTIBODY (AB6982) ab6982 staining Adenovirus type 5 in human HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton and then blocked using 0.1% BSA for 16 hours at 4°C. Samples were then incubated with primary antibody at 1/800 for 1 hour at 37°C. The secondary antibodyused was a donkey
ANTI-INTERFERON GAMMA ANTIBODY (AB9657) This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIFN-gamma. Can be paired for ELISA with ab9658. WB. Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 17 kDa. RECOMBINANT ANTI-OCCLUDIN ANTIBODY KO TESTED Immunoprecipitation - Anti-Occludin antibody (ab216327) Occludin was immunoprecipitated from 0.35 mg of Caco-2 (human colorectal adenocarcinoma cell line) whole cell lysate with ab216327 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab216327 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP RECOMBINANT ANTI-GSDMD ANTIBODY KO TESTED All lanes : Anti-GSDMD antibody (ab210070) at 1/1000 dilution. Lane 1 : THP-1 (Human monocytic leukemia monocyte) cells were differentiated into macrophage-like cells by incubation in the presence of 50ng/ul PMA for 48h. Then incubated for 90 min without treatment whole cell lysate. ANTIBODIES, PROTEINS, KITS AND REAGENTS FOR LIFE SCIENCEPRIMARY ANTIBODIESSECONDARY ANTIBODIESELISA AND MATCHED ANTIBODY PAIR KITS Abcam, the leading supplier of protein research tools to life scientists. Discover more from a range of 118,000 antibodies, kits, proteins and other reagents ANTI-COLLAGEN I ANTIBODY (AB34710) Anti-Collagen I antibody (ab34710) is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. This collagen antibody was developed using non-denatured 3D epitopes, you must be careful not to denature the collagen WESTER BLOT PROTOCOL FOR HIGH MOLECULAR WEIGHT PROTEINS Immerse the gel in 1× transfer buffer for 40 min. Activate the PVDF membrane with 99.5% methanol for 15 seconds. Immerse PVDF membrane, filter paper and sponge in 1× transfer buffer for 30 min before transfer. Complete a wet transfer at 500 mA, for 1h, at 4°C using pre-chilled transfer buffer. Once complete, wash twice for 10 minutesin
PRODUCT DATA AND DOCUMENTS A Safety Data sheets (SDS) or Material Safety Data Sheet (MSDS) details the safety considerations for a particular substance. This includes the physical, health and environmental hazards, protective measures and safety precautions for handling, storing, and transporting that substance. Many SDS documents are available on theproduct's online
ANTI-COLLAGEN III ANTIBODY (AB7778) Anti-Collagen III antibody (ab184993) Research with confidence – consistent and reproducible results with every batch. Long-term and scalable supply – powered by recombinant technology for fast production. Success from the first experiment – confirmed specificity through extensive validation. Ethical standards compliant RECOMBINANT ANTI-BDNF ANTIBODY (AB108319) Immunohistochemistry (Frozen sections) - Anti-BDNF antibody (ab108319) Immunohistochemistry (Frozen sections) analysis of mouse cerebrum tissue sections labeling BDNF with Purified ab108319 at 1/100 (2.8 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). ANTI-ADENOVIRUS TYPE 5 ANTIBODY (AB6982) ab6982 staining Adenovirus type 5 in human HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton and then blocked using 0.1% BSA for 16 hours at 4°C. Samples were then incubated with primary antibody at 1/800 for 1 hour at 37°C. The secondary antibodyused was a donkey
ANTI-INTERFERON GAMMA ANTIBODY (AB9657) This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIFN-gamma. Can be paired for ELISA with ab9658. WB. Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 17 kDa. RECOMBINANT ANTI-OCCLUDIN ANTIBODY KO TESTED Immunoprecipitation - Anti-Occludin antibody (ab216327) Occludin was immunoprecipitated from 0.35 mg of Caco-2 (human colorectal adenocarcinoma cell line) whole cell lysate with ab216327 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab216327 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP RECOMBINANT ANTI-GSDMD ANTIBODY KO TESTED All lanes : Anti-GSDMD antibody (ab210070) at 1/1000 dilution. Lane 1 : THP-1 (Human monocytic leukemia monocyte) cells were differentiated into macrophage-like cells by incubation in the presence of 50ng/ul PMA for 48h. Then incubated for 90 min without treatment whole cell lysate. ANTI-COLLAGEN III ANTIBODY (AB7778) Anti-Collagen III antibody (ab184993) Research with confidence – consistent and reproducible results with every batch. Long-term and scalable supply – powered by recombinant technology for fast production. Success from the first experiment – confirmed specificity through extensive validation. Ethical standards compliant ANTI-ADENOVIRUS TYPE 5 ANTIBODY (AB6982) ab6982 staining Adenovirus type 5 in human HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton and then blocked using 0.1% BSA for 16 hours at 4°C. Samples were then incubated with primary antibody at 1/800 for 1 hour at 37°C. The secondary antibodyused was a donkey
ANTI-LAMININ ANTIBODY (AB11575) Immunoblotting. Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane was separated on SDS-PAGE and probed with Rabbit Anti-Laminin ab11575. The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase secondary antibody and a chemiluminescent substrate. Lanes. 1. antibody dilution 1/1,000. ANTI-MONOAMINE OXIDASE B/MAOB ANTIBODY (AB88510) General notes. This product was previously labelled as Monoamine Oxidase B. The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. ANTI-TLR4 ANTIBODY (AB13556) Immunohistochemistry (Frozen sections) - Anti-TLR4 antibody (ab13556) ab13556 at 1/100000 dilution (12 hrs at 4degC) staining TLR4 in mouse colitis colon tissue section by Immunohistochemistry (Formalin-fixed tissue sections). A biotin Goat Anti-Rabbit secondary was used at 1/2000 for 1 hour at RT.DCFDA / H2DCFDA
The DCFDA assay protocol is based on the diffusion of DCFDA / H2DCFDA / DCFH-DA / DCFH into the cell. It is then deacetylated by cellular esterases to a non-fluorescent compound, which is later oxidized by ROS into 2’, 7’ –dichlorofluorescein (DCF). DCF is highly fluorescent and is detected by fluorescence spectroscopy withexcitation
ANTIBODY DILUTIONS AND TITER The optimal antibody concentration, which gives the best staining with minimum background, must be determined experimentally for each assay and is usually determined by using a series of dilutions in a titration experiment. For example, if a product datasheet suggests using a 1:200 dilution, it is recommended to make dilutions of 1:50,1:100, 1
RECOMBINANT ANTI-SOX2 ANTIBODY (AB92494) IHC - Wholemount - Anti-SOX2 antibody (ab92494) This image is courtesy of an anonymous Abreview. IHC - Wholemount analysis of mouse blastocyst labelling SOX2 (pink) with unpurified ab92494 at 1/200. The sample was incubated with the primary antibody for RECOMBINANT ANTI-NEUN ANTIBODY Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum tissue labelling NeuN with ab177487 at 1/100 dilution (B), SOX1 with ab242125 at 1/100 dilution (C) and Olig2 with ab109186 at 1/100 dilution (D). Anti-Rabbit and Mouse Polymer HRP was used as a secondary antibody, and DAPI was used for a nuclearcounter stain.
PHOSPHO ALPHA-SYNUCLEIN (S129) ANTIBODY [EP1536Y Western blot - Anti-Alpha-synuclein (phospho S129) antibody (ab51253) Different batches of ab51253 were tested on mouse brain lysate at 3.0 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 18 kDa. Western blot - Anti-Alpha-synuclein (phospho S129) antibody (ab51253) This image is courtesy of Professor ANTIBODIES, PROTEINS, KITS AND REAGENTS FOR LIFE SCIENCEPRIMARY ANTIBODIESSECONDARY ANTIBODIESELISA AND MATCHED ANTIBODY PAIR KITS Abcam, the leading supplier of protein research tools to life scientists. Discover more from a range of 118,000 antibodies, kits, proteins and other reagents PRODUCT DATA AND DOCUMENTS A Safety Data sheets (SDS) or Material Safety Data Sheet (MSDS) details the safety considerations for a particular substance. This includes the physical, health and environmental hazards, protective measures and safety precautions for handling, storing, and transporting that substance. Many SDS documents are available on theproduct's online
WESTER BLOT PROTOCOL FOR HIGH MOLECULAR WEIGHT PROTEINS Immerse the gel in 1× transfer buffer for 40 min. Activate the PVDF membrane with 99.5% methanol for 15 seconds. Immerse PVDF membrane, filter paper and sponge in 1× transfer buffer for 30 min before transfer. Complete a wet transfer at 500 mA, for 1h, at 4°C using pre-chilled transfer buffer. Once complete, wash twice for 10 minutesin
ANTI-SARS-COV-2 SPIKE GLYCOPROTEIN ANTIBODY ELISA - Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus (ab272504) A direct ELISA was performed using antigen or control peptide as coating antigen and ab272504 as the capture antibody at 1 μg/ml, followed by the secondary antibody goat anti-rabbit IgG HRP conjugate at 1/20000 dilution. Detection range was from 0.5 ng/ml to1000 ng/ml.
ANTIBODIES, PROTEINS, KITS AND REAGENTS FOR LIFE SCIENCEPRIMARY ANTIBODIESSECONDARY ANTIBODIESELISA AND MATCHED ANTIBODY PAIR KITS Abcam, the leading supplier of protein research tools to life scientists. Discover more from a range of 118,000 antibodies, kits, proteins and other reagents PRODUCT DATA AND DOCUMENTS A Safety Data sheets (SDS) or Material Safety Data Sheet (MSDS) details the safety considerations for a particular substance. This includes the physical, health and environmental hazards, protective measures and safety precautions for handling, storing, and transporting that substance. Many SDS documents are available on theproduct's online
WESTER BLOT PROTOCOL FOR HIGH MOLECULAR WEIGHT PROTEINS Immerse the gel in 1× transfer buffer for 40 min. Activate the PVDF membrane with 99.5% methanol for 15 seconds. Immerse PVDF membrane, filter paper and sponge in 1× transfer buffer for 30 min before transfer. Complete a wet transfer at 500 mA, for 1h, at 4°C using pre-chilled transfer buffer. Once complete, wash twice for 10 minutesin
ANTI-SARS-COV-2 SPIKE GLYCOPROTEIN ANTIBODY ELISA - Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus (ab272504) A direct ELISA was performed using antigen or control peptide as coating antigen and ab272504 as the capture antibody at 1 μg/ml, followed by the secondary antibody goat anti-rabbit IgG HRP conjugate at 1/20000 dilution. Detection range was from 0.5 ng/ml to1000 ng/ml.
PRODUCT DATA AND DOCUMENTS A Safety Data sheets (SDS) or Material Safety Data Sheet (MSDS) details the safety considerations for a particular substance. This includes the physical, health and environmental hazards, protective measures and safety precautions for handling, storing, and transporting that substance. Many SDS documents are available on theproduct's online
PROTOCOLS BOOK
Immunohistochemistry. Flow Cytometry. ELISA. Immunoprecipitation. ChIP. Buffers. Stock solutions. This book is designed for anyone working in a lab who wants tried and tested protocols for these techniques. It also includes information on choosing an antibody andstorage guides.
ANTI-TGF BETA 1 ANTIBODY (AB92486) ab92486 was shown to react with TGF beta 1 in western blot. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab92486 and ab8245 (Mouse anti-GAPDH antibody ) overnight at 4°C at 5 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L(IRDye
ANTI-COLLAGEN I ANTIBODY (AB34710) Anti-Collagen I antibody (ab34710) is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. This collagen antibody was developed using non-denatured 3D epitopes, you must be careful not to denature the collagen ANTI-ADENOVIRUS TYPE 5 ANTIBODY (AB6982) ab6982 staining Adenovirus type 5 in human HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton and then blocked using 0.1% BSA for 16 hours at 4°C. Samples were then incubated with primary antibody at 1/800 for 1 hour at 37°C. The secondary antibodyused was a donkey
ANTI-TET2 ANTIBODY (AB94580) Immunocytochemistry - Anti-Tet2 antibody (ab94580) ab94580 staining TET2 in MCF7 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. VERIBLOT FOR IP SECONDARY ANTIBODY (HRP) (AB131366) Lane 3 (-): Rabbit monoclonal IgG ( ab172730) instead of ab124962 in NIH/3T3 whole cell lysate. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST. Dilutingbuffer
RECOMBINANT ANTI-PRAME ANTIBODY (AB219650) PRAME (PReferentially expressed Antigen in MElanoma) is a tumor-associated antigen and is a member of the family of cancer testis antigens (CTA). PRAME is expressed in malignant cells, including leukaemias, Hodgkin's lymphoma, breast cancer, and primary and metastatic melanomas. For more information, please refer to PMID:27441500 .
RESULTS FOR "CD206"
Anti-Mannose Receptor antibody (ab64693) Reviews (34) Specific References (302) Description: Rabbit polyclonal to Mannose Receptor. Application: ICC/IF, IHC-P, WB. Reactivity: Mouse, Rat, Human (predicted: Chimpanzee, Rhesus monkey) Conjugate: PHOSPHO MYOSIN LIGHT CHAIN (S20) ANTIBODY (AB2480) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain (phospho S20) antibody (ab2480) IHC-P of ab2480 at 2.5 µg/ml staining both vascular and myometrial smooth muscle cells of the human uterus. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purplenuclear
ANTIBODIES, PROTEINS, KITS AND REAGENTS FOR LIFE SCIENCEPRIMARY ANTIBODIESSECONDARY ANTIBODIESELISA AND MATCHED ANTIBODY PAIR KITS Abcam, the leading supplier of protein research tools to life scientists. Discover more from a range of 118,000 antibodies, kits, proteins and other reagents PROTOCOLS AND TROUBLESHOOTING TIPS Immunoprecipitation protocol. IP troubleshooting tips. RNA Immunoprecipitation (RIP) Using IgM antibodies for IP. UV Cross-Linking and Immunoprecipitation (CLIP) Immunostaining (19) +. BrdU immunostaining procedure for cell cultures and tissue sections. GMA (Glycol methacrylate) embedding for immmunohistochemistryprotocol.
ANTIBODY STORAGE GUIDE ANTI-HA TAG ANTIBODY The ChIP was performed with 25µg of chromatin, 20µl of Protein A/G sepharose beads, and 3µg of ab9110 (anti-HA, light blue) or, 3µg of ab18337 (anti-Boris, dark blue). A non-specific antibody was used as a control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). ChIP - Anti-HA tag antibody - ChIP Grade CHOOSING AN ANTIBODY Choosing a secondary antibody. Secondary antibodies should be against the host species of the primary antibody you are using. For example, if your primary is a mouse monoclonal, you will require an anti-mouse secondary. Check the datasheet of the secondary antibody to ensure it is tested in the application you will be using. IHC ANTIGEN RETRIEVAL PROTOCOL Method. Deparaffinize and rehydrate the sections. Use a sufficient volume of antigen retrieval solution to cover the slides by at least a few centimeters. Add the appropriate antigen retrieval buffer to the microwaveable vessel. Use a non-sealed vessel to allow for evaporationduring the boil.
WESTERN BLOT PROTOCOL Western blotting is a technique that uses specific antibodies to identify proteins that have been separated based on size by gel electrophoresis. The immunoassay uses a membrane made of nitrocellulose or PVDF (polyvinylidene fluoride). The gel is placed next to the membrane and the application of an electrical current induces the proteins to ANTI-COLLAGEN I ANTIBODY (AB34710) Anti-Collagen I antibody (ab34710) is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. This collagen antibody was developed using non-denatured 3D epitopes, you must be careful not to denature the collagen ANTI-FIBRILLARIN ANTIBODY Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody - Nucleolar Marker (ab5821) Lab. ab5821 staining Fibrillarin in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. VERIBLOT FOR IP SECONDARY ANTIBODY (HRP) (AB131366) Lane 3 (-): Rabbit monoclonal IgG ( ab172730) instead of ab124962 in NIH/3T3 whole cell lysate. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST. Dilutingbuffer
ANTIBODIES, PROTEINS, KITS AND REAGENTS FOR LIFE SCIENCEPRIMARY ANTIBODIESSECONDARY ANTIBODIESELISA AND MATCHED ANTIBODY PAIR KITS Abcam, the leading supplier of protein research tools to life scientists. Discover more from a range of 118,000 antibodies, kits, proteins and other reagents PROTOCOLS AND TROUBLESHOOTING TIPS Immunoprecipitation protocol. IP troubleshooting tips. RNA Immunoprecipitation (RIP) Using IgM antibodies for IP. UV Cross-Linking and Immunoprecipitation (CLIP) Immunostaining (19) +. BrdU immunostaining procedure for cell cultures and tissue sections. GMA (Glycol methacrylate) embedding for immmunohistochemistryprotocol.
ANTIBODY STORAGE GUIDE ANTI-HA TAG ANTIBODY The ChIP was performed with 25µg of chromatin, 20µl of Protein A/G sepharose beads, and 3µg of ab9110 (anti-HA, light blue) or, 3µg of ab18337 (anti-Boris, dark blue). A non-specific antibody was used as a control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). ChIP - Anti-HA tag antibody - ChIP Grade CHOOSING AN ANTIBODY Choosing a secondary antibody. Secondary antibodies should be against the host species of the primary antibody you are using. For example, if your primary is a mouse monoclonal, you will require an anti-mouse secondary. Check the datasheet of the secondary antibody to ensure it is tested in the application you will be using. IHC ANTIGEN RETRIEVAL PROTOCOL Method. Deparaffinize and rehydrate the sections. Use a sufficient volume of antigen retrieval solution to cover the slides by at least a few centimeters. Add the appropriate antigen retrieval buffer to the microwaveable vessel. Use a non-sealed vessel to allow for evaporationduring the boil.
WESTERN BLOT PROTOCOL Western blotting is a technique that uses specific antibodies to identify proteins that have been separated based on size by gel electrophoresis. The immunoassay uses a membrane made of nitrocellulose or PVDF (polyvinylidene fluoride). The gel is placed next to the membrane and the application of an electrical current induces the proteins to ANTI-COLLAGEN I ANTIBODY (AB34710) Anti-Collagen I antibody (ab34710) is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. This collagen antibody was developed using non-denatured 3D epitopes, you must be careful not to denature the collagen ANTI-FIBRILLARIN ANTIBODY Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody - Nucleolar Marker (ab5821) Lab. ab5821 staining Fibrillarin in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. VERIBLOT FOR IP SECONDARY ANTIBODY (HRP) (AB131366) Lane 3 (-): Rabbit monoclonal IgG ( ab172730) instead of ab124962 in NIH/3T3 whole cell lysate. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST. Dilutingbuffer
ABCAM - ANTIBODIES AND REAGENTS SUPPLIER, FIND ANY ANTIBODY You can manage your marketing preferences by clicking the link at the bottom of every email we send. If you need further help with your account, please contact customer services on (888) 77-ABCAM (22226) contact us. or orders@abcam.com. PROTOCOLS AND TROUBLESHOOTING TIPS Immunoprecipitation protocol. IP troubleshooting tips. RNA Immunoprecipitation (RIP) Using IgM antibodies for IP. UV Cross-Linking and Immunoprecipitation (CLIP) Immunostaining (19) +. BrdU immunostaining procedure for cell cultures and tissue sections. GMA (Glycol methacrylate) embedding for immmunohistochemistryprotocol.
IHC ANTIGEN RETRIEVAL PROTOCOL Method. Deparaffinize and rehydrate the sections. Use a sufficient volume of antigen retrieval solution to cover the slides by at least a few centimeters. Add the appropriate antigen retrieval buffer to the microwaveable vessel. Use a non-sealed vessel to allow for evaporationduring the boil.
IN SITU HYBRIDIZATION (ISH) PROTOCOL 4) Immerse slides in ice-cold 20% (v/v) acetic acid for 20 sec. This permeabilizes the cells to allow access to the probe and the antibody. 5) Dehydrate the slides by washing for approximately 1 min per wash in 70% ethanol, 95% ethanol and 100% ethanol, then air dry. 6) CHOOSING AN ANTIBODY Choosing a secondary antibody. Secondary antibodies should be against the host species of the primary antibody you are using. For example, if your primary is a mouse monoclonal, you will require an anti-mouse secondary. Check the datasheet of the secondary antibody to ensure it is tested in the application you will be using. ANTI-COLLAGEN I ANTIBODY (AB34710) Anti-Collagen I antibody (ab34710) is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. This collagen antibody was developed using non-denatured 3D epitopes, you must be careful not to denature the collagen FLUORESCENCE COMPENSATION IN FLOW CYTOMETRY Within a flow cytometer, the appropriate ranges of excitation and emission wavelengths are selected by bandpass filters. However, when emission spectra overlap, fluorescence from more than one fluorochrome may be detected. To correct for this spectral overlap, a process of fluorescence compensation is used. This ensures that the fluorescence ANTI-TGF BETA 1 ANTIBODY (AB92486) ab92486 was shown to react with TGF beta 1 in western blot. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab92486 and ab8245 (Mouse anti-GAPDH antibody ) overnight at 4°C at 5 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L(IRDye
RNA ISOLATION AND PROTOCOL: CELLS IN CULTURE RNA isolation procedure for tissue. The only difference from the procedure in cells is the first step. Add 1 ml TRIzol to a sterile culture tube (preferably 12x75 mm). To this tube, add the frozen tissue (try not to add more than approximately 20 mg). On ice, pulverize the tissue with a homogenizer at a setting of 25 out of 30for a total of 2
ANTI-NEUN ANTIBODY
Mouse monoclonal NeuN antibody - Neuronal Marker. Validated in WB, IHC, ICC and tested in Mouse, Rat, Human. Cited in 257 publication(s). Independently ANTIBODIES, PROTEINS, KITS AND REAGENTS FOR LIFE SCIENCEPRIMARY ANTIBODIESSECONDARY ANTIBODIESELISA AND MATCHED ANTIBODY PAIR KITS Abcam, the leading supplier of protein research tools to life scientists. Discover more from a range of 118,000 antibodies, kits, proteins and other reagents PROTOCOLS AND TROUBLESHOOTING TIPS Immunoprecipitation protocol. IP troubleshooting tips. RNA Immunoprecipitation (RIP) Using IgM antibodies for IP. UV Cross-Linking and Immunoprecipitation (CLIP) Immunostaining (19) +. BrdU immunostaining procedure for cell cultures and tissue sections. GMA (Glycol methacrylate) embedding for immmunohistochemistryprotocol.
ANTIBODY STORAGE GUIDE ANTI-HA TAG ANTIBODY The ChIP was performed with 25µg of chromatin, 20µl of Protein A/G sepharose beads, and 3µg of ab9110 (anti-HA, light blue) or, 3µg of ab18337 (anti-Boris, dark blue). A non-specific antibody was used as a control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). ChIP - Anti-HA tag antibody - ChIP Grade CHOOSING AN ANTIBODY Choosing a secondary antibody. Secondary antibodies should be against the host species of the primary antibody you are using. For example, if your primary is a mouse monoclonal, you will require an anti-mouse secondary. Check the datasheet of the secondary antibody to ensure it is tested in the application you will be using. IHC ANTIGEN RETRIEVAL PROTOCOL Method. Deparaffinize and rehydrate the sections. Use a sufficient volume of antigen retrieval solution to cover the slides by at least a few centimeters. Add the appropriate antigen retrieval buffer to the microwaveable vessel. Use a non-sealed vessel to allow for evaporationduring the boil.
WESTERN BLOT PROTOCOL Western blotting is a technique that uses specific antibodies to identify proteins that have been separated based on size by gel electrophoresis. The immunoassay uses a membrane made of nitrocellulose or PVDF (polyvinylidene fluoride). The gel is placed next to the membrane and the application of an electrical current induces the proteins to ANTI-COLLAGEN I ANTIBODY (AB34710) Anti-Collagen I antibody (ab34710) is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. This collagen antibody was developed using non-denatured 3D epitopes, you must be careful not to denature the collagen ANTI-FIBRILLARIN ANTIBODY Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody - Nucleolar Marker (ab5821) Lab. ab5821 staining Fibrillarin in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. VERIBLOT FOR IP SECONDARY ANTIBODY (HRP) (AB131366) Lane 3 (-): Rabbit monoclonal IgG ( ab172730) instead of ab124962 in NIH/3T3 whole cell lysate. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST. Dilutingbuffer
ANTIBODIES, PROTEINS, KITS AND REAGENTS FOR LIFE SCIENCEPRIMARY ANTIBODIESSECONDARY ANTIBODIESELISA AND MATCHED ANTIBODY PAIR KITS Abcam, the leading supplier of protein research tools to life scientists. Discover more from a range of 118,000 antibodies, kits, proteins and other reagents PROTOCOLS AND TROUBLESHOOTING TIPS Immunoprecipitation protocol. IP troubleshooting tips. RNA Immunoprecipitation (RIP) Using IgM antibodies for IP. UV Cross-Linking and Immunoprecipitation (CLIP) Immunostaining (19) +. BrdU immunostaining procedure for cell cultures and tissue sections. GMA (Glycol methacrylate) embedding for immmunohistochemistryprotocol.
ANTIBODY STORAGE GUIDE ANTI-HA TAG ANTIBODY The ChIP was performed with 25µg of chromatin, 20µl of Protein A/G sepharose beads, and 3µg of ab9110 (anti-HA, light blue) or, 3µg of ab18337 (anti-Boris, dark blue). A non-specific antibody was used as a control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). ChIP - Anti-HA tag antibody - ChIP Grade CHOOSING AN ANTIBODY Choosing a secondary antibody. Secondary antibodies should be against the host species of the primary antibody you are using. For example, if your primary is a mouse monoclonal, you will require an anti-mouse secondary. Check the datasheet of the secondary antibody to ensure it is tested in the application you will be using. IHC ANTIGEN RETRIEVAL PROTOCOL Method. Deparaffinize and rehydrate the sections. Use a sufficient volume of antigen retrieval solution to cover the slides by at least a few centimeters. Add the appropriate antigen retrieval buffer to the microwaveable vessel. Use a non-sealed vessel to allow for evaporationduring the boil.
WESTERN BLOT PROTOCOL Western blotting is a technique that uses specific antibodies to identify proteins that have been separated based on size by gel electrophoresis. The immunoassay uses a membrane made of nitrocellulose or PVDF (polyvinylidene fluoride). The gel is placed next to the membrane and the application of an electrical current induces the proteins to ANTI-COLLAGEN I ANTIBODY (AB34710) Anti-Collagen I antibody (ab34710) is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. This collagen antibody was developed using non-denatured 3D epitopes, you must be careful not to denature the collagen ANTI-FIBRILLARIN ANTIBODY Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody - Nucleolar Marker (ab5821) Lab. ab5821 staining Fibrillarin in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. VERIBLOT FOR IP SECONDARY ANTIBODY (HRP) (AB131366) Lane 3 (-): Rabbit monoclonal IgG ( ab172730) instead of ab124962 in NIH/3T3 whole cell lysate. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST. Dilutingbuffer
ABCAM - ANTIBODIES AND REAGENTS SUPPLIER, FIND ANY ANTIBODY You can manage your marketing preferences by clicking the link at the bottom of every email we send. If you need further help with your account, please contact customer services on (888) 77-ABCAM (22226) contact us. or orders@abcam.com. PROTOCOLS AND TROUBLESHOOTING TIPS Immunoprecipitation protocol. IP troubleshooting tips. RNA Immunoprecipitation (RIP) Using IgM antibodies for IP. UV Cross-Linking and Immunoprecipitation (CLIP) Immunostaining (19) +. BrdU immunostaining procedure for cell cultures and tissue sections. GMA (Glycol methacrylate) embedding for immmunohistochemistryprotocol.
IHC ANTIGEN RETRIEVAL PROTOCOL Method. Deparaffinize and rehydrate the sections. Use a sufficient volume of antigen retrieval solution to cover the slides by at least a few centimeters. Add the appropriate antigen retrieval buffer to the microwaveable vessel. Use a non-sealed vessel to allow for evaporationduring the boil.
IN SITU HYBRIDIZATION (ISH) PROTOCOL 4) Immerse slides in ice-cold 20% (v/v) acetic acid for 20 sec. This permeabilizes the cells to allow access to the probe and the antibody. 5) Dehydrate the slides by washing for approximately 1 min per wash in 70% ethanol, 95% ethanol and 100% ethanol, then air dry. 6) CHOOSING AN ANTIBODY Choosing a secondary antibody. Secondary antibodies should be against the host species of the primary antibody you are using. For example, if your primary is a mouse monoclonal, you will require an anti-mouse secondary. Check the datasheet of the secondary antibody to ensure it is tested in the application you will be using. ANTI-COLLAGEN I ANTIBODY (AB34710) Anti-Collagen I antibody (ab34710) is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. This collagen antibody was developed using non-denatured 3D epitopes, you must be careful not to denature the collagen FLUORESCENCE COMPENSATION IN FLOW CYTOMETRY Within a flow cytometer, the appropriate ranges of excitation and emission wavelengths are selected by bandpass filters. However, when emission spectra overlap, fluorescence from more than one fluorochrome may be detected. To correct for this spectral overlap, a process of fluorescence compensation is used. This ensures that the fluorescence ANTI-TGF BETA 1 ANTIBODY (AB92486) ab92486 was shown to react with TGF beta 1 in western blot. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab92486 and ab8245 (Mouse anti-GAPDH antibody ) overnight at 4°C at 5 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L(IRDye
RNA ISOLATION AND PROTOCOL: CELLS IN CULTURE RNA isolation procedure for tissue. The only difference from the procedure in cells is the first step. Add 1 ml TRIzol to a sterile culture tube (preferably 12x75 mm). To this tube, add the frozen tissue (try not to add more than approximately 20 mg). On ice, pulverize the tissue with a homogenizer at a setting of 25 out of 30for a total of 2
ANTI-NEUN ANTIBODY
Mouse monoclonal NeuN antibody - Neuronal Marker. Validated in WB, IHC, ICC and tested in Mouse, Rat, Human. Cited in 257 publication(s). Independently Your browser does not have JavaScript enabled and some parts of this website will not work without it. For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome We use cookies to make our site as useful as possible. Our Cookie Policy explains how you can opt-out of the cookies we use. If you continue without changing your cookie settings, we'll assume you’re happy with this.Continue Continue
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